Echeneis naucrates (Live sharksucker)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Neoteleostei; Eurypterygia; Ctenosquamata; Acanthomorphata;

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54685 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A665TI60|A0A665TI60_ECHNA Isoform of A0A665TI39 Hsp90 co-chaperone Cdc37 OS=Echeneis naucrates OX=173247 PE=3 SV=1
VV1 pKa = 6.86YY2 pKa = 9.75DD3 pKa = 3.81YY4 pKa = 11.4QRR6 pKa = 11.84LCLQLGVAEE15 pKa = 4.76LVVDD19 pKa = 3.95NCRR22 pKa = 11.84SSDD25 pKa = 3.84GEE27 pKa = 4.42VEE29 pKa = 4.16GLTDD33 pKa = 4.08EE34 pKa = 4.39YY35 pKa = 11.02TGLEE39 pKa = 4.32MLSMVNVGLSSLSKK53 pKa = 10.87LPLTLEE59 pKa = 4.11VSDD62 pKa = 3.77NTISGGLDD70 pKa = 3.07SLAEE74 pKa = 3.99KK75 pKa = 10.63CPNLTYY81 pKa = 11.17LNLSGNKK88 pKa = 9.14IKK90 pKa = 10.29EE91 pKa = 3.9LSSIKK96 pKa = 10.36VLQNLKK102 pKa = 10.02NLKK105 pKa = 10.38SLDD108 pKa = 3.95LYY110 pKa = 10.58SCEE113 pKa = 4.17VTSLEE118 pKa = 4.67DD119 pKa = 3.25YY120 pKa = 10.55RR121 pKa = 11.84EE122 pKa = 4.33SVFEE126 pKa = 4.89LLPQLTYY133 pKa = 11.03LDD135 pKa = 5.36GYY137 pKa = 9.91DD138 pKa = 3.64QEE140 pKa = 5.69DD141 pKa = 3.9NEE143 pKa = 4.98AVDD146 pKa = 3.9RR147 pKa = 11.84LNDD150 pKa = 4.75FILKK154 pKa = 9.98GVADD158 pKa = 4.37PNFVPDD164 pKa = 4.29EE165 pKa = 4.64DD166 pKa = 5.64DD167 pKa = 3.5EE168 pKa = 5.29AGPPGDD174 pKa = 5.73DD175 pKa = 4.93DD176 pKa = 6.64DD177 pKa = 7.04DD178 pKa = 6.23DD179 pKa = 4.69EE180 pKa = 5.12EE181 pKa = 4.99EE182 pKa = 4.42EE183 pKa = 5.01EE184 pKa = 4.63EE185 pKa = 5.37DD186 pKa = 4.27DD187 pKa = 5.59DD188 pKa = 5.19EE189 pKa = 4.96EE190 pKa = 5.99GSEE193 pKa = 4.11GDD195 pKa = 3.43EE196 pKa = 4.51DD197 pKa = 4.5EE198 pKa = 5.23VGLSYY203 pKa = 11.24LMKK206 pKa = 10.64EE207 pKa = 5.02GIQVSLPVSWSLISGLQHH225 pKa = 7.34APQCPGRR232 pKa = 11.84RR233 pKa = 11.84TLTAYY238 pKa = 10.28YY239 pKa = 9.37VLHH242 pKa = 6.83SGPSCCSRR250 pKa = 11.84SFIVFPGG257 pKa = 3.19

Molecular weight: 28.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A665UYY0|A0A665UYY0_ECHNA Arylformamidase OS=Echeneis naucrates OX=173247 GN=afmid PE=4 SV=1
PP1 pKa = 7.38RR2 pKa = 11.84RR3 pKa = 11.84SACWRR8 pKa = 11.84RR9 pKa = 11.84PKK11 pKa = 10.15RR12 pKa = 11.84SKK14 pKa = 10.72KK15 pKa = 7.87SWRR18 pKa = 11.84TIVGPQTRR26 pKa = 11.84EE27 pKa = 3.7GKK29 pKa = 10.6GRR31 pKa = 11.84GAQTRR36 pKa = 11.84GGKK39 pKa = 9.79GRR41 pKa = 11.84GAQTRR46 pKa = 11.84GGKK49 pKa = 9.79GRR51 pKa = 11.84GAQTRR56 pKa = 11.84GGKK59 pKa = 9.79GRR61 pKa = 11.84GAQTRR66 pKa = 11.84GGKK69 pKa = 9.79GRR71 pKa = 11.84GAQTRR76 pKa = 11.84GGKK79 pKa = 9.79GRR81 pKa = 11.84GAQTRR86 pKa = 11.84GGKK89 pKa = 9.75GRR91 pKa = 11.84GPQTRR96 pKa = 11.84GGKK99 pKa = 9.76GRR101 pKa = 11.84GPQTRR106 pKa = 11.84GGKK109 pKa = 9.75GRR111 pKa = 11.84GPQRR115 pKa = 11.84RR116 pKa = 11.84GGKK119 pKa = 9.8GRR121 pKa = 11.84GAQTRR126 pKa = 11.84GTKK129 pKa = 9.71GG130 pKa = 2.91

Molecular weight: 13.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21548

33137

54685

36544125

17

8621

668.3

74.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.423 ± 0.009

2.311 ± 0.009

5.258 ± 0.006

6.779 ± 0.011

3.838 ± 0.007

6.219 ± 0.012

2.667 ± 0.005

4.711 ± 0.007

5.719 ± 0.01

9.794 ± 0.013

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.432 ± 0.004

3.958 ± 0.005

5.384 ± 0.011

4.7 ± 0.01

5.512 ± 0.007

8.243 ± 0.012

5.569 ± 0.008

6.406 ± 0.008

1.182 ± 0.003

2.892 ± 0.005
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski