Amino acid dipepetide frequency for Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.748AlaAla: 11.748 ± 0.14	1.201AlaCys: 1.201 ± 0.028	5.396AlaAsp: 5.396 ± 0.062	5.978AlaGlu: 5.978 ± 0.077	3.801AlaPhe: 3.801 ± 0.053	8.318AlaGly: 8.318 ± 0.084	1.982AlaHis: 1.982 ± 0.038	6.128AlaIle: 6.128 ± 0.065	3.615AlaLys: 3.615 ± 0.064	12.957AlaLeu: 12.957 ± 0.103	3.19AlaMet: 3.19 ± 0.048	2.901AlaAsn: 2.901 ± 0.042	4.069AlaPro: 4.069 ± 0.056	4.724AlaGln: 4.724 ± 0.057	6.317AlaArg: 6.317 ± 0.074	5.744AlaSer: 5.744 ± 0.068	4.884AlaThr: 4.884 ± 0.063	7.33AlaVal: 7.33 ± 0.082	1.901AlaTrp: 1.901 ± 0.035	1.924AlaTyr: 1.924 ± 0.036	0.0AlaXaa: 0.0 ± 0.0
Cys	1.042CysAla: 1.042 ± 0.022	0.171CysCys: 0.171 ± 0.01	0.583CysAsp: 0.583 ± 0.021	0.566CysGlu: 0.566 ± 0.021	0.445CysPhe: 0.445 ± 0.017	1.103CysGly: 1.103 ± 0.029	0.303CysHis: 0.303 ± 0.016	0.536CysIle: 0.536 ± 0.019	0.302CysLys: 0.302 ± 0.014	1.04CysLeu: 1.04 ± 0.028	0.232CysMet: 0.232 ± 0.011	0.288CysAsn: 0.288 ± 0.014	0.537CysPro: 0.537 ± 0.018	0.443CysGln: 0.443 ± 0.018	0.696CysArg: 0.696 ± 0.022	0.656CysSer: 0.656 ± 0.021	0.468CysThr: 0.468 ± 0.017	0.732CysVal: 0.732 ± 0.021	0.194CysTrp: 0.194 ± 0.012	0.32CysTyr: 0.32 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	5.315AspAla: 5.315 ± 0.06	0.486AspCys: 0.486 ± 0.017	2.958AspAsp: 2.958 ± 0.046	3.448AspGlu: 3.448 ± 0.046	2.038AspPhe: 2.038 ± 0.038	3.779AspGly: 3.779 ± 0.055	1.004AspHis: 1.004 ± 0.024	3.284AspIle: 3.284 ± 0.043	2.379AspLys: 2.379 ± 0.04	4.763AspLeu: 4.763 ± 0.053	1.289AspMet: 1.289 ± 0.033	2.082AspAsn: 2.082 ± 0.038	2.426AspPro: 2.426 ± 0.038	1.553AspGln: 1.553 ± 0.034	2.906AspArg: 2.906 ± 0.048	3.529AspSer: 3.529 ± 0.805	2.391AspThr: 2.391 ± 0.046	3.606AspVal: 3.606 ± 0.049	0.832AspTrp: 0.832 ± 0.027	1.818AspTyr: 1.818 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	5.784GluAla: 5.784 ± 0.067	0.453GluCys: 0.453 ± 0.02	2.248GluAsp: 2.248 ± 0.045	3.285GluGlu: 3.285 ± 0.058	1.727GluPhe: 1.727 ± 0.036	3.649GluGly: 3.649 ± 0.051	1.332GluHis: 1.332 ± 0.028	3.079GluIle: 3.079 ± 0.049	2.991GluLys: 2.991 ± 0.054	5.697GluLeu: 5.697 ± 0.071	1.781GluMet: 1.781 ± 0.032	2.076GluAsn: 2.076 ± 0.035	2.092GluPro: 2.092 ± 0.04	3.28GluGln: 3.28 ± 0.047	3.708GluArg: 3.708 ± 0.05	2.861GluSer: 2.861 ± 0.042	2.862GluThr: 2.862 ± 0.048	3.752GluVal: 3.752 ± 0.054	0.782GluTrp: 0.782 ± 0.021	1.395GluTyr: 1.395 ± 0.029	0.0GluXaa: 0.0 ± 0.0
Phe	3.952PheAla: 3.952 ± 0.057	0.534PheCys: 0.534 ± 0.019	2.271PheAsp: 2.271 ± 0.041	1.646PheGlu: 1.646 ± 0.032	1.729PhePhe: 1.729 ± 0.045	3.218PheGly: 3.218 ± 0.057	0.837PheHis: 0.837 ± 0.025	2.56PheIle: 2.56 ± 0.048	1.141PheLys: 1.141 ± 0.027	3.31PheLeu: 3.31 ± 0.057	0.967PheMet: 0.967 ± 0.027	1.633PheAsn: 1.633 ± 0.033	1.58PhePro: 1.58 ± 0.036	1.142PheGln: 1.142 ± 0.026	1.921PheArg: 1.921 ± 0.035	3.066PheSer: 3.066 ± 0.047	2.445PheThr: 2.445 ± 0.039	2.549PheVal: 2.549 ± 0.038	0.661PheTrp: 0.661 ± 0.019	1.198PheTyr: 1.198 ± 0.031	0.0PheXaa: 0.0 ± 0.0
Gly	6.698GlyAla: 6.698 ± 0.078	1.005GlyCys: 1.005 ± 0.025	3.705GlyAsp: 3.705 ± 0.055	4.766GlyGlu: 4.766 ± 0.059	3.337GlyPhe: 3.337 ± 0.054	5.699GlyGly: 5.699 ± 0.06	1.656GlyHis: 1.656 ± 0.033	5.043GlyIle: 5.043 ± 0.066	3.797GlyLys: 3.797 ± 0.049	7.931GlyLeu: 7.931 ± 0.081	2.483GlyMet: 2.483 ± 0.046	2.562GlyAsn: 2.562 ± 0.045	2.204GlyPro: 2.204 ± 0.033	3.059GlyGln: 3.059 ± 0.051	4.011GlyArg: 4.011 ± 0.055	4.238GlySer: 4.238 ± 0.057	3.63GlyThr: 3.63 ± 0.049	5.935GlyVal: 5.935 ± 0.068	1.364GlyTrp: 1.364 ± 0.029	2.655GlyTyr: 2.655 ± 0.043	0.0GlyXaa: 0.0 ± 0.0
His	1.984HisAla: 1.984 ± 0.039	0.301HisCys: 0.301 ± 0.011	1.251HisAsp: 1.251 ± 0.031	1.041HisGlu: 1.041 ± 0.027	1.042HisPhe: 1.042 ± 0.025	1.849HisGly: 1.849 ± 0.034	0.809HisHis: 0.809 ± 0.024	1.278HisIle: 1.278 ± 0.031	0.72HisLys: 0.72 ± 0.021	2.369HisLeu: 2.369 ± 0.042	0.516HisMet: 0.516 ± 0.018	0.768HisAsn: 0.768 ± 0.021	1.37HisPro: 1.37 ± 0.033	1.213HisGln: 1.213 ± 0.027	1.329HisArg: 1.329 ± 0.033	1.249HisSer: 1.249 ± 0.029	1.063HisThr: 1.063 ± 0.024	1.281HisVal: 1.281 ± 0.027	0.414HisTrp: 0.414 ± 0.018	0.917HisTyr: 0.917 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	6.763IleAla: 6.763 ± 0.078	0.667IleCys: 0.667 ± 0.021	3.491IleAsp: 3.491 ± 0.05	2.982IleGlu: 2.982 ± 0.05	2.042IlePhe: 2.042 ± 0.037	4.626IleGly: 4.626 ± 0.065	1.184IleHis: 1.184 ± 0.028	3.219IleIle: 3.219 ± 0.052	2.083IleLys: 2.083 ± 0.042	4.861IleLeu: 4.861 ± 0.067	1.196IleMet: 1.196 ± 0.028	2.393IleAsn: 2.393 ± 0.041	2.765IlePro: 2.765 ± 0.039	1.689IleGln: 1.689 ± 0.029	2.993IleArg: 2.993 ± 0.046	3.522IleSer: 3.522 ± 0.052	3.236IleThr: 3.236 ± 0.048	3.985IleVal: 3.985 ± 0.048	0.673IleTrp: 0.673 ± 0.02	1.48IleTyr: 1.48 ± 0.033	0.0IleXaa: 0.0 ± 0.0
Lys	4.181LysAla: 4.181 ± 0.061	0.24LysCys: 0.24 ± 0.014	1.806LysAsp: 1.806 ± 0.039	2.18LysGlu: 2.18 ± 0.046	1.015LysPhe: 1.015 ± 0.026	2.753LysGly: 2.753 ± 0.047	0.774LysHis: 0.774 ± 0.026	2.091LysIle: 2.091 ± 0.04	1.984LysLys: 1.984 ± 0.046	3.877LysLeu: 3.877 ± 0.056	1.173LysMet: 1.173 ± 0.028	1.476LysAsn: 1.476 ± 0.034	1.954LysPro: 1.954 ± 0.034	1.801LysGln: 1.801 ± 0.037	2.373LysArg: 2.373 ± 0.043	2.073LysSer: 2.073 ± 0.034	2.346LysThr: 2.346 ± 0.046	2.806LysVal: 2.806 ± 0.042	0.415LysTrp: 0.415 ± 0.018	1.051LysTyr: 1.051 ± 0.026	0.0LysXaa: 0.0 ± 0.0
Leu	12.803LeuAla: 12.803 ± 0.124	1.282LeuCys: 1.282 ± 0.033	5.319LeuAsp: 5.319 ± 0.062	5.388LeuGlu: 5.388 ± 0.064	4.323LeuPhe: 4.323 ± 0.059	7.613LeuGly: 7.613 ± 0.076	2.333LeuHis: 2.333 ± 0.042	5.766LeuIle: 5.766 ± 0.077	4.173LeuLys: 4.173 ± 0.059	12.617LeuLeu: 12.617 ± 0.15	2.929LeuMet: 2.929 ± 0.049	4.02LeuAsn: 4.02 ± 0.054	5.955LeuPro: 5.955 ± 0.064	4.534LeuGln: 4.534 ± 0.058	6.631LeuArg: 6.631 ± 0.071	7.334LeuSer: 7.334 ± 0.078	6.464LeuThr: 6.464 ± 0.071	7.205LeuVal: 7.205 ± 0.073	1.542LeuTrp: 1.542 ± 0.035	2.53LeuTyr: 2.53 ± 0.039	0.0LeuXaa: 0.0 ± 0.0
Met	3.127MetAla: 3.127 ± 0.051	0.195MetCys: 0.195 ± 0.012	1.118MetAsp: 1.118 ± 0.026	1.204MetGlu: 1.204 ± 0.029	0.856MetPhe: 0.856 ± 0.024	1.909MetGly: 1.909 ± 0.04	0.548MetHis: 0.548 ± 0.018	1.438MetIle: 1.438 ± 0.033	1.311MetLys: 1.311 ± 0.03	3.189MetLeu: 3.189 ± 0.05	0.932MetMet: 0.932 ± 0.025	1.07MetAsn: 1.07 ± 0.029	1.351MetPro: 1.351 ± 0.026	1.162MetGln: 1.162 ± 0.029	1.52MetArg: 1.52 ± 0.031	1.809MetSer: 1.809 ± 0.035	1.828MetThr: 1.828 ± 0.034	1.967MetVal: 1.967 ± 0.036	0.269MetTrp: 0.269 ± 0.015	0.511MetTyr: 0.511 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	3.289AsnAla: 3.289 ± 0.051	0.303AsnCys: 0.303 ± 0.014	1.931AsnAsp: 1.931 ± 0.037	1.713AsnGlu: 1.713 ± 0.035	1.216AsnPhe: 1.216 ± 0.031	2.87AsnGly: 2.87 ± 0.047	0.819AsnHis: 0.819 ± 0.027	2.107AsnIle: 2.107 ± 0.042	1.367AsnLys: 1.367 ± 0.037	3.28AsnLeu: 3.28 ± 0.047	0.862AsnMet: 0.862 ± 0.025	1.389AsnAsn: 1.389 ± 0.035	2.027AsnPro: 2.027 ± 0.038	1.438AsnGln: 1.438 ± 0.03	1.908AsnArg: 1.908 ± 0.037	1.769AsnSer: 1.769 ± 0.038	1.771AsnThr: 1.771 ± 0.035	2.443AsnVal: 2.443 ± 0.041	0.513AsnTrp: 0.513 ± 0.02	1.086AsnTyr: 1.086 ± 0.03	0.0AsnXaa: 0.0 ± 0.0
Pro	5.128ProAla: 5.128 ± 0.062	0.389ProCys: 0.389 ± 0.015	2.766ProAsp: 2.766 ± 0.042	3.29ProGlu: 3.29 ± 0.047	1.921ProPhe: 1.921 ± 0.035	3.713ProGly: 3.713 ± 0.045	1.121ProHis: 1.121 ± 0.031	1.771ProIle: 1.771 ± 0.038	1.321ProLys: 1.321 ± 0.03	5.566ProLeu: 5.566 ± 0.069	1.082ProMet: 1.082 ± 0.026	1.184ProAsn: 1.184 ± 0.03	1.89ProPro: 1.89 ± 0.039	2.587ProGln: 2.587 ± 0.041	2.253ProArg: 2.253 ± 0.039	2.169ProSer: 2.169 ± 0.04	2.157ProThr: 2.157 ± 0.041	3.95ProVal: 3.95 ± 0.045	0.857ProTrp: 0.857 ± 0.023	1.176ProTyr: 1.176 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	5.153GlnAla: 5.153 ± 0.066	0.366GlnCys: 0.366 ± 0.016	1.733GlnAsp: 1.733 ± 0.034	2.155GlnGlu: 2.155 ± 0.038	1.461GlnPhe: 1.461 ± 0.026	3.197GlnGly: 3.197 ± 0.049	1.258GlnHis: 1.258 ± 0.028	2.163GlnIle: 2.163 ± 0.033	1.641GlnLys: 1.641 ± 0.036	5.113GlnLeu: 5.113 ± 0.064	1.194GlnMet: 1.194 ± 0.029	1.274GlnAsn: 1.274 ± 0.026	2.351GlnPro: 2.351 ± 0.05	3.417GlnGln: 3.417 ± 0.069	3.366GlnArg: 3.366 ± 0.051	2.236GlnSer: 2.236 ± 0.041	2.172GlnThr: 2.172 ± 0.035	3.059GlnVal: 3.059 ± 0.049	0.699GlnTrp: 0.699 ± 0.025	1.171GlnTyr: 1.171 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	5.067ArgAla: 5.067 ± 0.061	0.643ArgCys: 0.643 ± 0.022	3.174ArgAsp: 3.174 ± 0.04	3.985ArgGlu: 3.985 ± 0.057	2.679ArgPhe: 2.679 ± 0.043	3.658ArgGly: 3.658 ± 0.059	1.763ArgHis: 1.763 ± 0.032	3.279ArgIle: 3.279 ± 0.046	2.215ArgLys: 2.215 ± 0.04	7.035ArgLeu: 7.035 ± 0.075	1.614ArgMet: 1.614 ± 0.032	1.893ArgAsn: 1.893 ± 0.03	2.511ArgPro: 2.511 ± 0.042	3.517ArgGln: 3.517 ± 0.057	3.959ArgArg: 3.959 ± 0.067	2.839ArgSer: 2.839 ± 0.042	2.446ArgThr: 2.446 ± 0.035	3.945ArgVal: 3.945 ± 0.048	1.113ArgTrp: 1.113 ± 0.03	2.256ArgTyr: 2.256 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	5.811SerAla: 5.811 ± 0.066	0.6SerCys: 0.6 ± 0.02	3.832SerAsp: 3.832 ± 0.812	3.054SerGlu: 3.054 ± 0.043	2.263SerPhe: 2.263 ± 0.037	5.218SerGly: 5.218 ± 0.068	1.414SerHis: 1.414 ± 0.031	2.827SerIle: 2.827 ± 0.044	1.842SerLys: 1.842 ± 0.038	6.771SerLeu: 6.771 ± 0.074	1.462SerMet: 1.462 ± 0.028	1.674SerAsn: 1.674 ± 0.038	2.69SerPro: 2.69 ± 0.042	2.506SerGln: 2.506 ± 0.041	3.562SerArg: 3.562 ± 0.054	3.308SerSer: 3.308 ± 0.06	2.781SerThr: 2.781 ± 0.042	4.078SerVal: 4.078 ± 0.053	1.046SerTrp: 1.046 ± 0.028	1.578SerTyr: 1.578 ± 0.034	0.0SerXaa: 0.0 ± 0.0
Thr	5.109ThrAla: 5.109 ± 0.059	0.509ThrCys: 0.509 ± 0.017	2.42ThrAsp: 2.42 ± 0.041	2.413ThrGlu: 2.413 ± 0.036	2.03ThrPhe: 2.03 ± 0.038	4.387ThrGly: 4.387 ± 0.052	1.158ThrHis: 1.158 ± 0.027	2.827ThrIle: 2.827 ± 0.045	1.259ThrLys: 1.259 ± 0.032	7.555ThrLeu: 7.555 ± 0.057	1.148ThrMet: 1.148 ± 0.028	1.358ThrAsn: 1.358 ± 0.026	3.243ThrPro: 3.243 ± 0.041	1.892ThrGln: 1.892 ± 0.036	3.159ThrArg: 3.159 ± 0.048	2.765ThrSer: 2.765 ± 0.04	2.827ThrThr: 2.827 ± 0.044	3.874ThrVal: 3.874 ± 0.05	0.859ThrTrp: 0.859 ± 0.024	1.115ThrTyr: 1.115 ± 0.032	0.0ThrXaa: 0.0 ± 0.0
Val	7.548ValAla: 7.548 ± 0.078	0.773ValCys: 0.773 ± 0.02	3.721ValAsp: 3.721 ± 0.055	3.965ValGlu: 3.965 ± 0.049	2.581ValPhe: 2.581 ± 0.042	4.989ValGly: 4.989 ± 0.069	1.257ValHis: 1.257 ± 0.028	4.409ValIle: 4.409 ± 0.054	2.903ValLys: 2.903 ± 0.052	7.254ValLeu: 7.254 ± 0.077	2.225ValMet: 2.225 ± 0.033	2.662ValAsn: 2.662 ± 0.045	3.126ValPro: 3.126 ± 0.045	2.394ValGln: 2.394 ± 0.038	3.819ValArg: 3.819 ± 0.058	4.609ValSer: 4.609 ± 0.055	4.138ValThr: 4.138 ± 0.045	5.696ValVal: 5.696 ± 0.067	1.031ValTrp: 1.031 ± 0.028	1.721ValTyr: 1.721 ± 0.031	0.0ValXaa: 0.0 ± 0.0
Trp	1.059TrpAla: 1.059 ± 0.026	0.183TrpCys: 0.183 ± 0.011	0.642TrpAsp: 0.642 ± 0.024	0.604TrpGlu: 0.604 ± 0.021	0.73TrpPhe: 0.73 ± 0.021	0.996TrpGly: 0.996 ± 0.027	0.471TrpHis: 0.471 ± 0.016	0.727TrpIle: 0.727 ± 0.021	0.491TrpLys: 0.491 ± 0.021	2.558TrpLeu: 2.558 ± 0.043	0.46TrpMet: 0.46 ± 0.018	0.499TrpAsn: 0.499 ± 0.021	0.746TrpPro: 0.746 ± 0.024	1.272TrpGln: 1.272 ± 0.032	1.268TrpArg: 1.268 ± 0.029	0.87TrpSer: 0.87 ± 0.022	0.609TrpThr: 0.609 ± 0.021	0.983TrpVal: 0.983 ± 0.025	0.238TrpTrp: 0.238 ± 0.013	0.432TrpTyr: 0.432 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.546TyrAla: 2.546 ± 0.039	0.354TyrCys: 0.354 ± 0.016	1.521TyrAsp: 1.521 ± 0.032	1.173TyrGlu: 1.173 ± 0.027	1.122TyrPhe: 1.122 ± 0.026	2.235TyrGly: 2.235 ± 0.039	0.741TyrHis: 0.741 ± 0.02	1.319TyrIle: 1.319 ± 0.03	0.834TyrLys: 0.834 ± 0.022	3.019TyrLeu: 3.019 ± 0.04	0.578TyrMet: 0.578 ± 0.021	0.9TyrAsn: 0.9 ± 0.028	1.404TyrPro: 1.404 ± 0.033	1.558TyrGln: 1.558 ± 0.033	1.891TyrArg: 1.891 ± 0.037	1.671TyrSer: 1.671 ± 0.037	1.394TyrThr: 1.394 ± 0.028	1.646TyrVal: 1.646 ± 0.033	0.433TyrTrp: 0.433 ± 0.017	0.925TyrTyr: 0.925 ± 0.028	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5126 proteins (1601607 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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