Tobacco vein clearing virus (isolate Nicotiana edwardsonii/United States/Lockhart/2000) (TVCV)

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Caulimoviridae; Solendovirus; Tobacco vein clearing virus

Average proteome isoelectric point is 7.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q9QD06|Q9QD06_TVCVL Coat protein OS=Tobacco vein clearing virus (isolate Nicotiana edwardsonii/United States/Lockhart/2000) OX=1289468 PE=3 SV=1
MM1 pKa = 7.29NKK3 pKa = 9.92EE4 pKa = 3.58EE5 pKa = 3.95FAIDD9 pKa = 3.23EE10 pKa = 4.39KK11 pKa = 10.6TYY13 pKa = 10.94EE14 pKa = 4.17NQDD17 pKa = 3.09GLKK20 pKa = 10.16IKK22 pKa = 10.43IIFSNLGRR30 pKa = 11.84RR31 pKa = 11.84YY32 pKa = 10.28KK33 pKa = 10.75KK34 pKa = 10.44IGDD37 pKa = 3.75QIYY40 pKa = 11.08LMIEE44 pKa = 3.89KK45 pKa = 8.47EE46 pKa = 4.23TARR49 pKa = 11.84LEE51 pKa = 4.41DD52 pKa = 3.99SLTAMTRR59 pKa = 11.84IIKK62 pKa = 10.09EE63 pKa = 3.74NEE65 pKa = 3.9EE66 pKa = 3.59IDD68 pKa = 3.41KK69 pKa = 10.88KK70 pKa = 11.12RR71 pKa = 11.84EE72 pKa = 3.47IEE74 pKa = 4.3IIRR77 pKa = 11.84NQANKK82 pKa = 10.06EE83 pKa = 3.92IQQLEE88 pKa = 4.2EE89 pKa = 4.08TKK91 pKa = 9.21NTKK94 pKa = 10.06IIALEE99 pKa = 4.22KK100 pKa = 9.27EE101 pKa = 4.24LNMLKK106 pKa = 10.56LLYY109 pKa = 10.1EE110 pKa = 4.21NKK112 pKa = 9.44QRR114 pKa = 11.84EE115 pKa = 4.03KK116 pKa = 11.04DD117 pKa = 3.63KK118 pKa = 11.07EE119 pKa = 3.95IEE121 pKa = 4.12LTDD124 pKa = 4.33EE125 pKa = 3.92INKK128 pKa = 9.34FKK130 pKa = 11.04QKK132 pKa = 10.41LQPEE136 pKa = 4.19EE137 pKa = 3.68NLRR140 pKa = 11.84IEE142 pKa = 5.14EE143 pKa = 4.08IDD145 pKa = 4.68NNIDD149 pKa = 3.58DD150 pKa = 4.38QKK152 pKa = 11.6SEE154 pKa = 3.98LSEE157 pKa = 3.73ISEE160 pKa = 4.7TYY162 pKa = 10.27TEE164 pKa = 3.8ILEE167 pKa = 4.7NIEE170 pKa = 3.84KK171 pKa = 9.9TKK173 pKa = 10.74NLEE176 pKa = 3.93INTGDD181 pKa = 3.23VNMDD185 pKa = 4.01EE186 pKa = 4.93KK187 pKa = 10.97PSTSGIKK194 pKa = 9.88NPKK197 pKa = 8.41EE198 pKa = 4.03LNPSYY203 pKa = 9.95YY204 pKa = 9.19TVSYY208 pKa = 9.33EE209 pKa = 4.24QSDD212 pKa = 3.94RR213 pKa = 11.84NNTLWNSRR221 pKa = 11.84LNKK224 pKa = 9.73KK225 pKa = 6.57WTPKK229 pKa = 9.99PIHH232 pKa = 5.59EE233 pKa = 4.55QYY235 pKa = 11.56NFLDD239 pKa = 4.21LDD241 pKa = 3.99CVEE244 pKa = 5.83DD245 pKa = 3.67INKK248 pKa = 8.83TIQLWIGYY256 pKa = 9.01ISKK259 pKa = 10.16QLIDD263 pKa = 3.65NKK265 pKa = 10.46IGITEE270 pKa = 4.03TPGYY274 pKa = 9.94IEE276 pKa = 4.09RR277 pKa = 11.84TLIGTVKK284 pKa = 10.38LWLHH288 pKa = 6.09NLTKK292 pKa = 10.71EE293 pKa = 4.47SIDD296 pKa = 3.44TLRR299 pKa = 11.84SNKK302 pKa = 9.39KK303 pKa = 10.28FNGDD307 pKa = 3.05MATTSMEE314 pKa = 3.66ILYY317 pKa = 9.84KK318 pKa = 10.41YY319 pKa = 9.98EE320 pKa = 3.44IAIRR324 pKa = 11.84NEE326 pKa = 3.89FSSMTTEE333 pKa = 3.81TEE335 pKa = 3.98EE336 pKa = 3.8QHH338 pKa = 7.01KK339 pKa = 10.21EE340 pKa = 3.64KK341 pKa = 10.73QINRR345 pKa = 11.84NLMTKK350 pKa = 10.48LAICNMCYY358 pKa = 9.36IDD360 pKa = 5.37EE361 pKa = 4.4YY362 pKa = 9.24TCAFRR367 pKa = 11.84EE368 pKa = 4.21YY369 pKa = 10.12YY370 pKa = 10.55YY371 pKa = 10.95KK372 pKa = 10.25GTYY375 pKa = 7.49NTEE378 pKa = 3.68EE379 pKa = 3.82GRR381 pKa = 11.84EE382 pKa = 3.86IRR384 pKa = 11.84KK385 pKa = 9.8LYY387 pKa = 8.11FTKK390 pKa = 10.57LPEE393 pKa = 4.44PFSSKK398 pKa = 10.1IIKK401 pKa = 9.51DD402 pKa = 3.25WNEE405 pKa = 3.56AGLTDD410 pKa = 3.92TLGARR415 pKa = 11.84IKK417 pKa = 10.46FLQQWFVQLCEE428 pKa = 4.25KK429 pKa = 10.53YY430 pKa = 9.85KK431 pKa = 11.07EE432 pKa = 4.1EE433 pKa = 4.01IKK435 pKa = 10.1MEE437 pKa = 4.81KK438 pKa = 9.95ILIKK442 pKa = 10.5NLKK445 pKa = 8.3CCKK448 pKa = 10.33NKK450 pKa = 8.96TAPQFGCTDD459 pKa = 3.66KK460 pKa = 11.55YY461 pKa = 9.57NKK463 pKa = 9.24KK464 pKa = 9.59WKK466 pKa = 7.12TKK468 pKa = 9.42KK469 pKa = 9.74YY470 pKa = 8.88KK471 pKa = 9.96KK472 pKa = 9.68HH473 pKa = 5.88RR474 pKa = 11.84SKK476 pKa = 11.29YY477 pKa = 9.46KK478 pKa = 8.9YY479 pKa = 8.76KK480 pKa = 10.52KK481 pKa = 8.41PRR483 pKa = 11.84KK484 pKa = 8.48RR485 pKa = 11.84YY486 pKa = 8.5YY487 pKa = 9.67VKK489 pKa = 10.52NYY491 pKa = 7.26QAKK494 pKa = 10.24KK495 pKa = 9.49PFRR498 pKa = 11.84PKK500 pKa = 10.55KK501 pKa = 10.26KK502 pKa = 9.27LTEE505 pKa = 4.2CTCYY509 pKa = 10.48NCGKK513 pKa = 9.63LGHH516 pKa = 6.75IAKK519 pKa = 9.97DD520 pKa = 3.64CKK522 pKa = 10.8APKK525 pKa = 9.88NPKK528 pKa = 9.38KK529 pKa = 10.6KK530 pKa = 10.33QITEE534 pKa = 4.1IIIDD538 pKa = 3.97DD539 pKa = 4.18EE540 pKa = 5.3EE541 pKa = 4.68YY542 pKa = 10.36MQMEE546 pKa = 4.74YY547 pKa = 9.93IDD549 pKa = 4.54YY550 pKa = 10.44EE551 pKa = 4.42LDD553 pKa = 3.76SEE555 pKa = 4.4DD556 pKa = 4.11SIYY559 pKa = 10.68EE560 pKa = 3.79ISDD563 pKa = 3.3SDD565 pKa = 4.76DD566 pKa = 4.7EE567 pKa = 5.01IDD569 pKa = 4.38SEE571 pKa = 4.22ITEE574 pKa = 4.4EE575 pKa = 3.96EE576 pKa = 4.37DD577 pKa = 4.17EE578 pKa = 4.27II579 pKa = 6.24

Molecular weight:
69.08 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q9QD05|Q9QD05_TVCVL Putative cell-to-cell movement protein OS=Tobacco vein clearing virus (isolate Nicotiana edwardsonii/United States/Lockhart/2000) OX=1289468 PE=4 SV=1
MM1 pKa = 7.76KK2 pKa = 9.95IYY4 pKa = 10.16ILAKK8 pKa = 9.84IIVEE12 pKa = 4.68GYY14 pKa = 7.64YY15 pKa = 10.52NRR17 pKa = 11.84YY18 pKa = 5.62YY19 pKa = 10.91TPMIDD24 pKa = 3.21TGAEE28 pKa = 3.94ANICKK33 pKa = 10.01YY34 pKa = 10.96NCLPTDD40 pKa = 2.7KK41 pKa = 10.12WEE43 pKa = 4.11KK44 pKa = 10.57LKK46 pKa = 10.39TPMVVTGFNNEE57 pKa = 3.55GSMINYY63 pKa = 8.05KK64 pKa = 10.3ARR66 pKa = 11.84NVKK69 pKa = 9.16IQIWDD74 pKa = 3.82KK75 pKa = 10.56ILTIEE80 pKa = 4.47EE81 pKa = 4.27IYY83 pKa = 11.11NFEE86 pKa = 4.19FTTKK90 pKa = 10.94DD91 pKa = 3.28MLLGMPFLEE100 pKa = 4.44KK101 pKa = 10.31LYY103 pKa = 10.18PHH105 pKa = 7.77IITKK109 pKa = 5.99THH111 pKa = 4.35WWFTTPCKK119 pKa = 10.68NKK121 pKa = 10.06VGAKK125 pKa = 9.31RR126 pKa = 11.84VNNKK130 pKa = 7.37QRR132 pKa = 11.84KK133 pKa = 4.02TTEE136 pKa = 3.99WIRR139 pKa = 11.84GSEE142 pKa = 4.29KK143 pKa = 9.4ITQKK147 pKa = 11.04LEE149 pKa = 4.36NINKK153 pKa = 7.77NTTTQLEE160 pKa = 4.48IIIFTIDD167 pKa = 3.05KK168 pKa = 10.8VKK170 pKa = 10.49IIQKK174 pKa = 10.36KK175 pKa = 9.55LEE177 pKa = 3.98KK178 pKa = 10.37LYY180 pKa = 11.06NDD182 pKa = 4.09NPLQGWEE189 pKa = 3.85KK190 pKa = 10.67HH191 pKa = 4.0KK192 pKa = 9.91TKK194 pKa = 10.89VKK196 pKa = 10.4IEE198 pKa = 4.45LIEE201 pKa = 4.25EE202 pKa = 3.95NSIITQKK209 pKa = 9.58PLKK212 pKa = 10.73YY213 pKa = 10.34NFNDD217 pKa = 3.07LTEE220 pKa = 4.39FKK222 pKa = 10.57IHH224 pKa = 7.21IKK226 pKa = 10.67DD227 pKa = 3.75LLDD230 pKa = 3.42NKK232 pKa = 10.22YY233 pKa = 10.03IQEE236 pKa = 4.38SNSKK240 pKa = 8.07HH241 pKa = 5.22TSPAFIVNKK250 pKa = 10.11HH251 pKa = 5.18SEE253 pKa = 4.14QKK255 pKa = 9.79RR256 pKa = 11.84GKK258 pKa = 9.08SRR260 pKa = 11.84MVIDD264 pKa = 4.04YY265 pKa = 10.56RR266 pKa = 11.84NLNAKK271 pKa = 7.98TKK273 pKa = 8.87TYY275 pKa = 10.75NYY277 pKa = 7.77PIPNKK282 pKa = 9.55ILKK285 pKa = 9.19IRR287 pKa = 11.84QIQGYY292 pKa = 9.56NYY294 pKa = 9.96FSKK297 pKa = 10.61FDD299 pKa = 4.02CKK301 pKa = 10.9SGFYY305 pKa = 10.02HH306 pKa = 7.35LKK308 pKa = 10.93LEE310 pKa = 4.62DD311 pKa = 3.65EE312 pKa = 4.66SKK314 pKa = 11.06KK315 pKa = 9.77LTAFTVPQGFYY326 pKa = 9.41EE327 pKa = 4.28WNVLPFGYY335 pKa = 10.23KK336 pKa = 9.53NAPGRR341 pKa = 11.84YY342 pKa = 6.04QHH344 pKa = 6.91FMDD347 pKa = 4.7NYY349 pKa = 9.34FNQLEE354 pKa = 4.15NCIVYY359 pKa = 10.02IDD361 pKa = 5.64DD362 pKa = 3.36ILLYY366 pKa = 10.93SRR368 pKa = 11.84TQDD371 pKa = 3.14EE372 pKa = 4.81HH373 pKa = 7.93IKK375 pKa = 10.58LLEE378 pKa = 4.27KK379 pKa = 10.0FAHH382 pKa = 6.54IIEE385 pKa = 4.4NSGISLSKK393 pKa = 9.83TKK395 pKa = 11.0AEE397 pKa = 3.73IMKK400 pKa = 10.06NQIEE404 pKa = 4.17FLGIQIDD411 pKa = 3.82KK412 pKa = 11.11NGIKK416 pKa = 8.84MQTHH420 pKa = 6.44IVQKK424 pKa = 10.46IINLDD429 pKa = 3.6EE430 pKa = 5.32NIDD433 pKa = 3.95TKK435 pKa = 11.26KK436 pKa = 10.64KK437 pKa = 9.4LQSFLGIVNQVRR449 pKa = 11.84EE450 pKa = 4.78YY451 pKa = 10.52IPKK454 pKa = 9.98LAEE457 pKa = 3.88NLKK460 pKa = 10.03PLQKK464 pKa = 10.38KK465 pKa = 9.34LKK467 pKa = 10.02KK468 pKa = 10.1DD469 pKa = 3.28VEE471 pKa = 4.3YY472 pKa = 11.37SFDD475 pKa = 3.92EE476 pKa = 4.36KK477 pKa = 11.22DD478 pKa = 3.05KK479 pKa = 11.27EE480 pKa = 4.13QIKK483 pKa = 10.16KK484 pKa = 10.37IKK486 pKa = 9.72ILCKK490 pKa = 10.23KK491 pKa = 10.06LPKK494 pKa = 10.31LYY496 pKa = 10.72FPDD499 pKa = 3.63EE500 pKa = 4.39NKK502 pKa = 10.17KK503 pKa = 8.68FTYY506 pKa = 9.92IVEE509 pKa = 4.44TDD511 pKa = 3.28SSNYY515 pKa = 10.37SYY517 pKa = 11.63GGVLKK522 pKa = 10.7YY523 pKa = 10.35RR524 pKa = 11.84YY525 pKa = 8.69NKK527 pKa = 9.98EE528 pKa = 4.13KK529 pKa = 10.28IEE531 pKa = 3.99HH532 pKa = 5.78HH533 pKa = 6.1CRR535 pKa = 11.84YY536 pKa = 10.05YY537 pKa = 10.68SGSYY541 pKa = 9.4TEE543 pKa = 4.76PQEE546 pKa = 3.65KK547 pKa = 9.45WEE549 pKa = 4.18INRR552 pKa = 11.84KK553 pKa = 8.0EE554 pKa = 4.06LFALYY559 pKa = 10.07KK560 pKa = 10.4CLLAFEE566 pKa = 4.93PYY568 pKa = 9.5IVYY571 pKa = 9.05TRR573 pKa = 11.84FIVRR577 pKa = 11.84TDD579 pKa = 3.16NTQVKK584 pKa = 7.71WWITRR589 pKa = 11.84KK590 pKa = 9.67VQDD593 pKa = 3.37SVTTKK598 pKa = 10.13EE599 pKa = 3.62IRR601 pKa = 11.84RR602 pKa = 11.84LVLNILNFTFTIEE615 pKa = 4.96IINTNKK621 pKa = 10.13NVVADD626 pKa = 3.77YY627 pKa = 10.86LSRR630 pKa = 11.84QSYY633 pKa = 9.84PNN635 pKa = 3.3

Molecular weight:
75.69 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2050

417

635

512.5

60.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.024 ± 0.387

1.463 ± 0.209

4.439 ± 0.499

9.902 ± 1.288

2.927 ± 0.535

3.073 ± 0.21

1.561 ± 0.298

11.61 ± 1.333

12.146 ± 1.178

7.659 ± 0.413

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.805 ± 0.272

7.317 ± 0.347

3.366 ± 0.313

4.39 ± 0.437

3.805 ± 0.231

4.683 ± 0.439

7.024 ± 0.486

3.073 ± 0.686

1.073 ± 0.223

5.659 ± 0.26

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski