Tribolium castaneum (Red flour beetle)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Coleoptera; Polyphaga; Cucujiformia; Tenebrionoidea; Tenebrionidae;

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18505 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A139WAN1|A0A139WAN1_TRICA Heat shock protein 83 OS=Tribolium castaneum OX=7070 GN=TcasGA2_TC031438 PE=3 SV=1
MM1 pKa = 7.0YY2 pKa = 9.66RR3 pKa = 11.84ALHH6 pKa = 5.78LTDD9 pKa = 5.22LSPEE13 pKa = 3.69FSGNYY18 pKa = 5.78TCKK21 pKa = 10.37VSGPEE26 pKa = 4.4SEE28 pKa = 5.66DD29 pKa = 3.48EE30 pKa = 4.27VTKK33 pKa = 10.76TMIIYY38 pKa = 7.69EE39 pKa = 4.34TPDD42 pKa = 3.72DD43 pKa = 4.42DD44 pKa = 5.22LEE46 pKa = 4.27LRR48 pKa = 11.84ISQEE52 pKa = 3.75DD53 pKa = 3.98SEE55 pKa = 4.91IKK57 pKa = 10.44CSSEE61 pKa = 3.9GLFPQPNMTLYY72 pKa = 10.36IMNYY76 pKa = 9.67NMTEE80 pKa = 3.79ILDD83 pKa = 3.8QEE85 pKa = 4.12NDD87 pKa = 3.35EE88 pKa = 5.21LIDD91 pKa = 4.4RR92 pKa = 11.84GDD94 pKa = 3.93DD95 pKa = 3.46GLFNVSTVLVYY106 pKa = 10.81NPEE109 pKa = 4.02EE110 pKa = 3.96LTEE113 pKa = 4.0TFYY116 pKa = 10.99YY117 pKa = 9.91ICEE120 pKa = 4.04LSIPGTPFIINEE132 pKa = 4.25TMIHH136 pKa = 5.57FQDD139 pKa = 3.49NNEE142 pKa = 3.96IQVQEE147 pKa = 4.5DD148 pKa = 3.9FNLFNSTNSTDD159 pKa = 3.5STFEE163 pKa = 3.63EE164 pKa = 4.5SKK166 pKa = 10.82EE167 pKa = 3.64IEE169 pKa = 4.16TQIEE173 pKa = 3.9EE174 pKa = 4.48EE175 pKa = 4.3YY176 pKa = 11.38DD177 pKa = 3.82FNTTTAEE184 pKa = 4.14SATNATVADD193 pKa = 4.55DD194 pKa = 5.9DD195 pKa = 5.36GDD197 pKa = 4.24DD198 pKa = 4.83DD199 pKa = 4.37EE200 pKa = 5.14STRR203 pKa = 11.84QEE205 pKa = 3.98INEE208 pKa = 4.17SSSFRR213 pKa = 11.84HH214 pKa = 5.84LSSLITSFLTTALLLSQLYY233 pKa = 8.89FF234 pKa = 3.98

Molecular weight: 26.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A139WI97|A0A139WI97_TRICA Uncharacterized protein OS=Tribolium castaneum OX=7070 GN=TcasGA2_TC032998 PE=4 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.12WRR6 pKa = 11.84KK7 pKa = 9.1KK8 pKa = 9.32RR9 pKa = 11.84MRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 10.08RR15 pKa = 11.84KK16 pKa = 7.85RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.6MRR21 pKa = 11.84ARR23 pKa = 11.84SKK25 pKa = 11.11

Molecular weight: 3.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16568

1937

18505

9078672

11

20792

490.6

55.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.805 ± 0.018

2.096 ± 0.052

5.312 ± 0.018

6.874 ± 0.048

4.163 ± 0.02

5.309 ± 0.024

2.39 ± 0.012

5.797 ± 0.017

7.079 ± 0.039

9.243 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.092 ± 0.01

5.121 ± 0.022

5.257 ± 0.036

4.155 ± 0.017

5.149 ± 0.018

7.599 ± 0.022

5.86 ± 0.019

6.396 ± 0.017

1.087 ± 0.008

3.213 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski