endosymbiont of Galathealinum brachiosum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Gammaproteobacteria incertae sedis; sulfur-oxidizing symbionts

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3414 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A370DC79|A0A370DC79_9GAMM Uncharacterized protein OS=endosymbiont of Galathealinum brachiosum OX=2200906 GN=DIZ80_09485 PE=4 SV=1
MM1 pKa = 7.48NIKK4 pKa = 10.61NIFSQVLFIFTVFVLVSCGGGSGDD28 pKa = 4.9AVTTTTGQFIDD39 pKa = 5.29DD40 pKa = 4.16PVQGLDD46 pKa = 3.59YY47 pKa = 10.26SCSSGTTGITNINGEE62 pKa = 4.45YY63 pKa = 9.19TCNTGDD69 pKa = 4.78DD70 pKa = 3.93VTFSIGPVTLGTLSAQSSFITPYY93 pKa = 11.22SLFPNDD99 pKa = 2.84TQAAVNLARR108 pKa = 11.84LLQSIDD114 pKa = 3.42SDD116 pKa = 4.38SNPNNNVITLSSSQLSLLPTNTDD139 pKa = 3.48FTDD142 pKa = 3.11INFVANIEE150 pKa = 4.23TALGITLIDD159 pKa = 3.46AMTAMSHH166 pKa = 6.78LYY168 pKa = 10.29DD169 pKa = 5.64AIILNGGNVPDD180 pKa = 5.06GGHH183 pKa = 6.88LPIASAGMDD192 pKa = 3.46LSVVVQNAIALDD204 pKa = 4.02GSGSYY209 pKa = 10.7DD210 pKa = 3.6ADD212 pKa = 3.84GDD214 pKa = 4.29SLTYY218 pKa = 8.83TWSIASKK225 pKa = 10.18PPGSVSFLFTGMNTSAPILTPDD247 pKa = 3.22VVGDD251 pKa = 3.72YY252 pKa = 10.2VAQLIVNDD260 pKa = 4.23GLVNSAADD268 pKa = 3.66TVTITSTVSGNVSDD282 pKa = 5.03SPPSLAWIKK291 pKa = 8.66EE292 pKa = 3.87VRR294 pKa = 11.84VEE296 pKa = 4.08TSGGQDD302 pKa = 2.75GGIAFDD308 pKa = 4.47SNNNLYY314 pKa = 9.17MAAEE318 pKa = 4.08SLIDD322 pKa = 4.64FNGDD326 pKa = 3.11LNAGYY331 pKa = 10.57QDD333 pKa = 3.44ILVMKK338 pKa = 9.56FDD340 pKa = 4.38AAGDD344 pKa = 4.04MLWSDD349 pKa = 3.59QTGTANTDD357 pKa = 3.48LVDD360 pKa = 6.27DD361 pKa = 4.02IAFDD365 pKa = 3.41KK366 pKa = 10.78SANVLYY372 pKa = 9.61VTGSVSLSLHH382 pKa = 5.34GQDD385 pKa = 4.71VIGDD389 pKa = 3.53RR390 pKa = 11.84DD391 pKa = 3.57IYY393 pKa = 10.43IISYY397 pKa = 9.7DD398 pKa = 3.56AEE400 pKa = 4.55GNRR403 pKa = 11.84LWTIMDD409 pKa = 3.78GTAQEE414 pKa = 4.07EE415 pKa = 4.38RR416 pKa = 11.84PYY418 pKa = 11.01DD419 pKa = 3.43IALDD423 pKa = 3.55SSGNIFIAGVTHH435 pKa = 7.18GNFDD439 pKa = 4.08GNTTPTGRR447 pKa = 11.84DD448 pKa = 2.73IFVAKK453 pKa = 10.4YY454 pKa = 9.78NNLGAKK460 pKa = 8.98IWTRR464 pKa = 11.84QLQGDD469 pKa = 3.23NWEE472 pKa = 4.15RR473 pKa = 11.84AEE475 pKa = 4.73GIAVDD480 pKa = 4.11SSDD483 pKa = 3.09NVYY486 pKa = 8.62ITGYY490 pKa = 8.8TGSDD494 pKa = 3.27EE495 pKa = 5.18LGGEE499 pKa = 4.33VTNGSINSFIIKK511 pKa = 9.83YY512 pKa = 10.32SNDD515 pKa = 2.93GSRR518 pKa = 11.84LWTRR522 pKa = 11.84LISSANSEE530 pKa = 4.15YY531 pKa = 10.87GYY533 pKa = 11.52GVATDD538 pKa = 3.67SNNNVFVTGMTSGDD552 pKa = 3.33LDD554 pKa = 4.02GNTNRR559 pKa = 11.84GGYY562 pKa = 9.41DD563 pKa = 3.15QFTAKK568 pKa = 10.71YY569 pKa = 7.19NTNGALIWTTLTGGTLTDD587 pKa = 3.54TANDD591 pKa = 3.27IVVDD595 pKa = 3.77SFGRR599 pKa = 11.84IYY601 pKa = 9.9ITGQIGKK608 pKa = 9.2SEE610 pKa = 4.19TGDD613 pKa = 3.01IMDD616 pKa = 4.15YY617 pKa = 10.51VLTMFDD623 pKa = 3.49TDD625 pKa = 4.36GEE627 pKa = 4.66EE628 pKa = 3.85VWTVQNTPEE637 pKa = 3.99YY638 pKa = 11.1GRR640 pKa = 11.84IRR642 pKa = 11.84DD643 pKa = 3.78DD644 pKa = 3.61RR645 pKa = 11.84GKK647 pKa = 10.09HH648 pKa = 5.33LALDD652 pKa = 3.77SAEE655 pKa = 4.09NIYY658 pKa = 11.28VNGTTYY664 pKa = 11.17GSFFSTGDD672 pKa = 3.48DD673 pKa = 3.24ASNKK677 pKa = 9.24VFVARR682 pKa = 11.84YY683 pKa = 7.69NKK685 pKa = 9.98SVAGGPYY692 pKa = 10.02SVGGYY697 pKa = 10.3VNDD700 pKa = 5.15AIGSFTLTNNGGDD713 pKa = 3.73DD714 pKa = 4.53LFILEE719 pKa = 5.11DD720 pKa = 3.91GVFTFDD726 pKa = 3.72TLLNDD731 pKa = 3.87GASYY735 pKa = 10.77NVEE738 pKa = 4.04ILDD741 pKa = 5.38GSDD744 pKa = 2.69TSQGHH749 pKa = 5.87DD750 pKa = 3.74CEE752 pKa = 4.46VLSGGSNGDD761 pKa = 2.97GSGTINGADD770 pKa = 3.32VTDD773 pKa = 4.27IEE775 pKa = 4.85VSCVGII781 pKa = 3.99

Molecular weight: 82.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A370DCS3|A0A370DCS3_9GAMM Uncharacterized protein OS=endosymbiont of Galathealinum brachiosum OX=2200906 GN=DIZ80_08780 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84IKK11 pKa = 10.5RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.96GFRR19 pKa = 11.84ARR21 pKa = 11.84SRR23 pKa = 11.84TVGGRR28 pKa = 11.84KK29 pKa = 8.24VLKK32 pKa = 10.25ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.31GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3414

0

3414

1105146

20

5022

323.7

36.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.482 ± 0.042

0.981 ± 0.015

6.094 ± 0.037

6.4 ± 0.049

4.087 ± 0.034

6.552 ± 0.049

2.167 ± 0.022

7.398 ± 0.035

6.161 ± 0.06

9.996 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.655 ± 0.025

5.13 ± 0.043

3.624 ± 0.032

3.938 ± 0.027

4.228 ± 0.033

7.03 ± 0.041

5.331 ± 0.046

6.569 ± 0.039

1.077 ± 0.015

3.101 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski