Tortoise microvirus 11

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V1FVW5|A0A4V1FVW5_9VIRU Uncharacterized protein OS=Tortoise microvirus 11 OX=2583110 PE=4 SV=1
MM1 pKa = 7.43SNKK4 pKa = 8.61KK5 pKa = 8.08TFGGDD10 pKa = 3.09RR11 pKa = 11.84LGTGAGFTVSQRR23 pKa = 11.84AYY25 pKa = 9.53EE26 pKa = 4.19RR27 pKa = 11.84SSHH30 pKa = 7.03DD31 pKa = 3.29LGNVFRR37 pKa = 11.84SNLGVGMIMPCYY49 pKa = 10.03VDD51 pKa = 4.03FVKK54 pKa = 10.85RR55 pKa = 11.84GDD57 pKa = 3.36VWEE60 pKa = 4.4KK61 pKa = 11.08DD62 pKa = 3.09ITQHH66 pKa = 6.91ILTHH70 pKa = 5.85PTNGPIFGSYY80 pKa = 7.41EE81 pKa = 3.65CRR83 pKa = 11.84VAVFTGDD90 pKa = 2.87VRR92 pKa = 11.84LYY94 pKa = 11.06NKK96 pKa = 9.54QLHH99 pKa = 6.4NNTKK103 pKa = 10.85GIGLDD108 pKa = 3.3MKK110 pKa = 10.94SVIFPKK116 pKa = 9.82MRR118 pKa = 11.84LNGRR122 pKa = 11.84NIQWAEE128 pKa = 3.97NQDD131 pKa = 3.97LNAQQVNQTSLLATTGIRR149 pKa = 11.84GLGTLEE155 pKa = 3.97KK156 pKa = 10.33ADD158 pKa = 4.14GIGRR162 pKa = 11.84VEE164 pKa = 3.99IQRR167 pKa = 11.84NAMWVMMYY175 pKa = 10.21YY176 pKa = 10.4EE177 pKa = 4.84IYY179 pKa = 10.5SEE181 pKa = 4.25YY182 pKa = 10.24FANKK186 pKa = 8.74QEE188 pKa = 4.38EE189 pKa = 4.28IGVVIGGSEE198 pKa = 4.22VKK200 pKa = 9.62GTASVDD206 pKa = 3.94RR207 pKa = 11.84IQGRR211 pKa = 11.84SIFSGSNEE219 pKa = 3.51WTITQNNIIEE229 pKa = 4.38PNGNAGLQPQHH240 pKa = 6.27TYY242 pKa = 10.78RR243 pKa = 11.84ITGSGIGEE251 pKa = 3.76QTVAVKK257 pKa = 10.19IANNTYY263 pKa = 10.43VLEE266 pKa = 4.54KK267 pKa = 9.87FDD269 pKa = 4.26KK270 pKa = 11.1FIDD273 pKa = 4.15FKK275 pKa = 11.54YY276 pKa = 10.05SASGTWLEE284 pKa = 4.03FTVAQGGPQTITADD298 pKa = 3.22ANGRR302 pKa = 11.84LFQASGRR309 pKa = 11.84EE310 pKa = 3.94ITMGTDD316 pKa = 2.85IEE318 pKa = 4.54LLEE321 pKa = 4.77FPLSNIDD328 pKa = 3.96DD329 pKa = 3.7MRR331 pKa = 11.84EE332 pKa = 3.78KK333 pKa = 11.04VFAQPKK339 pKa = 8.09TSPLIIGYY347 pKa = 10.03YY348 pKa = 10.16GDD350 pKa = 4.03EE351 pKa = 4.27KK352 pKa = 11.09HH353 pKa = 6.31GLPYY357 pKa = 10.78DD358 pKa = 3.54QVTGQTSLDD367 pKa = 3.63STGKK371 pKa = 9.9PNTTSNMNSITDD383 pKa = 3.6WAGLGIATYY392 pKa = 10.52KK393 pKa = 9.95ADD395 pKa = 4.87RR396 pKa = 11.84WQTWLNSEE404 pKa = 4.17WIEE407 pKa = 4.03NVNTISSVDD416 pKa = 3.43TSSGSFSMDD425 pKa = 2.64ALAVAKK431 pKa = 10.24RR432 pKa = 11.84LWKK435 pKa = 10.52FNNDD439 pKa = 2.76VVAGGGSYY447 pKa = 10.51QDD449 pKa = 2.62WLMAVDD455 pKa = 4.51GMEE458 pKa = 4.21TYY460 pKa = 10.25GAPEE464 pKa = 3.8MPVYY468 pKa = 10.59RR469 pKa = 11.84GGCSSMITFDD479 pKa = 3.72EE480 pKa = 4.93VISSSEE486 pKa = 4.09YY487 pKa = 10.38GDD489 pKa = 3.67QPLGTLGGQGVMKK502 pKa = 10.07NVKK505 pKa = 9.25GGHH508 pKa = 3.88VRR510 pKa = 11.84FRR512 pKa = 11.84AEE514 pKa = 4.03EE515 pKa = 3.67NGMIMVVVWITPIIDD530 pKa = 3.54YY531 pKa = 10.3YY532 pKa = 11.14QGNKK536 pKa = 7.65WFTKK540 pKa = 10.52LEE542 pKa = 4.14TMDD545 pKa = 6.06DD546 pKa = 2.9IHH548 pKa = 8.74KK549 pKa = 10.15PIFDD553 pKa = 5.02AIGFQEE559 pKa = 4.12KK560 pKa = 7.91TTDD563 pKa = 4.97QMAAWDD569 pKa = 4.4TIVNEE574 pKa = 4.96DD575 pKa = 3.2GTEE578 pKa = 4.07TFFSAGKK585 pKa = 8.91EE586 pKa = 4.18PAWTEE591 pKa = 3.36YY592 pKa = 6.32WTRR595 pKa = 11.84NNEE598 pKa = 4.0NYY600 pKa = 10.84GSFTRR605 pKa = 11.84HH606 pKa = 4.54NDD608 pKa = 2.72EE609 pKa = 4.86RR610 pKa = 11.84YY611 pKa = 8.3MVLNRR616 pKa = 11.84DD617 pKa = 3.24YY618 pKa = 11.64TMNEE622 pKa = 3.27EE623 pKa = 4.32DD624 pKa = 5.59GRR626 pKa = 11.84IEE628 pKa = 5.71DD629 pKa = 3.87LTTYY633 pKa = 10.52IDD635 pKa = 3.69PTKK638 pKa = 10.43FLYY641 pKa = 10.04PFAYY645 pKa = 9.24TGLQYY650 pKa = 11.31GPFWVQIGFDD660 pKa = 3.79TITRR664 pKa = 11.84RR665 pKa = 11.84IMSGAKK671 pKa = 9.08MPTLL675 pKa = 3.75

Molecular weight: 75.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W6J3|A0A4P8W6J3_9VIRU Uncharacterized protein OS=Tortoise microvirus 11 OX=2583110 PE=4 SV=1
MM1 pKa = 7.99RR2 pKa = 11.84NPGFGSRR9 pKa = 11.84KK10 pKa = 9.46RR11 pKa = 11.84LLKK14 pKa = 10.49INNSEE19 pKa = 4.14VGDD22 pKa = 4.02DD23 pKa = 4.63LMTKK27 pKa = 10.16LRR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84TEE33 pKa = 4.05DD34 pKa = 3.19NKK36 pKa = 11.59DD37 pKa = 3.18MNTPGRR43 pKa = 11.84PLLYY47 pKa = 9.42TEE49 pKa = 5.13RR50 pKa = 11.84KK51 pKa = 9.32HH52 pKa = 7.06GVIPEE57 pKa = 4.19GNPRR61 pKa = 11.84TDD63 pKa = 2.62RR64 pKa = 11.84WDD66 pKa = 3.29IAQEE70 pKa = 3.8ATDD73 pKa = 3.78YY74 pKa = 10.77IARR77 pKa = 11.84SKK79 pKa = 10.37AARR82 pKa = 11.84RR83 pKa = 11.84DD84 pKa = 3.71AFDD87 pKa = 3.57KK88 pKa = 11.42AGGTEE93 pKa = 4.16GEE95 pKa = 4.12RR96 pKa = 11.84KK97 pKa = 9.38ILAEE101 pKa = 3.65RR102 pKa = 11.84ASKK105 pKa = 11.08NEE107 pKa = 3.78NTT109 pKa = 4.25

Molecular weight: 12.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1752

70

675

250.3

28.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.336 ± 1.707

0.628 ± 0.19

4.338 ± 0.846

9.247 ± 1.356

3.025 ± 0.639

8.904 ± 0.905

1.427 ± 0.327

7.705 ± 0.439

8.847 ± 2.18

5.594 ± 0.475

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.253 ± 0.443

6.221 ± 0.282

2.74 ± 0.446

3.881 ± 0.621

5.422 ± 0.784

5.879 ± 0.68

6.906 ± 0.806

4.167 ± 0.835

1.769 ± 0.392

3.71 ± 0.599
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski