Tomato yellow leaf curl China virus (TYLCCNV)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q9QDJ8|AC4_TYLCC Protein C4 OS=Tomato yellow leaf curl China virus OX=185793 GN=C4 PE=3 SV=1
MM1 pKa = 8.06WDD3 pKa = 3.35PLLNEE8 pKa = 4.38FPEE11 pKa = 4.68TVHH14 pKa = 7.0GFRR17 pKa = 11.84CMLAIKK23 pKa = 9.86YY24 pKa = 8.69LQLVEE29 pKa = 4.22NTYY32 pKa = 11.22SPDD35 pKa = 3.18TLGYY39 pKa = 10.84DD40 pKa = 4.83LIRR43 pKa = 11.84DD44 pKa = 4.71LISVIRR50 pKa = 11.84ARR52 pKa = 11.84DD53 pKa = 3.43YY54 pKa = 11.29GEE56 pKa = 3.55TSRR59 pKa = 11.84RR60 pKa = 11.84YY61 pKa = 9.12CHH63 pKa = 5.35FHH65 pKa = 6.24SRR67 pKa = 11.84LEE69 pKa = 4.36GASPAEE75 pKa = 4.09LRR77 pKa = 11.84QPLYY81 pKa = 10.91GSCCCPHH88 pKa = 7.02CPRR91 pKa = 11.84HH92 pKa = 5.62QKK94 pKa = 9.31TNVVKK99 pKa = 10.2QAHH102 pKa = 5.68VPEE105 pKa = 4.48AHH107 pKa = 7.21DD108 pKa = 3.75VPDD111 pKa = 3.6VQKK114 pKa = 10.85PP115 pKa = 3.25

Molecular weight: 13.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q9DXE9|AV2_TYLCC Protein V2 OS=Tomato yellow leaf curl China virus OX=185793 GN=V2 PE=3 SV=1
MM1 pKa = 7.93AKK3 pKa = 9.9RR4 pKa = 11.84PADD7 pKa = 3.43IVISTPASKK16 pKa = 10.29VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 2.95SPYY27 pKa = 8.15TGRR30 pKa = 11.84VAAPTVRR37 pKa = 11.84VTRR40 pKa = 11.84RR41 pKa = 11.84QMWSNRR47 pKa = 11.84PMYY50 pKa = 9.32RR51 pKa = 11.84KK52 pKa = 9.5PMMYY56 pKa = 10.13RR57 pKa = 11.84MYY59 pKa = 10.46RR60 pKa = 11.84SPDD63 pKa = 3.21VPRR66 pKa = 11.84GCEE69 pKa = 4.34GPCKK73 pKa = 10.3VQSCEE78 pKa = 3.73ARR80 pKa = 11.84HH81 pKa = 5.9DD82 pKa = 3.84VSHH85 pKa = 6.41TGKK88 pKa = 10.39VICVSDD94 pKa = 3.43VTRR97 pKa = 11.84GNGITHH103 pKa = 6.78RR104 pKa = 11.84VGKK107 pKa = 9.5RR108 pKa = 11.84FCVKK112 pKa = 10.18SIYY115 pKa = 10.49VIGKK119 pKa = 8.28IWMDD123 pKa = 3.36EE124 pKa = 3.94NIKK127 pKa = 10.46FKK129 pKa = 11.18NHH131 pKa = 5.39TNTVMFFLVRR141 pKa = 11.84DD142 pKa = 3.97RR143 pKa = 11.84RR144 pKa = 11.84PSGTPMDD151 pKa = 4.2FQQVFNCYY159 pKa = 10.39DD160 pKa = 3.85NEE162 pKa = 4.32PSTATVKK169 pKa = 10.85NDD171 pKa = 2.82LRR173 pKa = 11.84DD174 pKa = 3.45RR175 pKa = 11.84FQVRR179 pKa = 11.84RR180 pKa = 11.84KK181 pKa = 9.75FYY183 pKa = 9.21STVTGGQYY191 pKa = 10.62ACKK194 pKa = 9.79EE195 pKa = 3.78QALVKK200 pKa = 10.51RR201 pKa = 11.84FIRR204 pKa = 11.84LNTHH208 pKa = 4.74VVYY211 pKa = 10.83NHH213 pKa = 5.75QEE215 pKa = 3.59QAKK218 pKa = 9.66YY219 pKa = 10.54EE220 pKa = 4.12NHH222 pKa = 6.33TEE224 pKa = 3.99NALLLYY230 pKa = 7.29MACTHH235 pKa = 7.07ASNPVYY241 pKa = 9.98ATLKK245 pKa = 9.08VRR247 pKa = 11.84IYY249 pKa = 10.66FYY251 pKa = 10.83DD252 pKa = 3.25SQMNN256 pKa = 3.43

Molecular weight: 29.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1097

97

361

182.8

21.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.831 ± 0.715

2.644 ± 0.453

4.923 ± 0.451

4.74 ± 0.643

4.649 ± 0.446

4.831 ± 0.488

4.376 ± 0.474

5.561 ± 0.871

5.743 ± 0.399

6.837 ± 0.951

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.37 ± 0.768

5.561 ± 0.624

5.834 ± 0.635

4.467 ± 0.223

7.11 ± 1.184

8.113 ± 1.394

6.108 ± 0.778

6.29 ± 1.027

1.094 ± 0.133

3.92 ± 0.617
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski