Trametes pubescens (White-rot fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Polyporales; Polyporaceae; Trametes

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14147 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M2V285|A0A1M2V285_TRAPU Zinc finger protein 2 OS=Trametes pubescens OX=154538 GN=TRAPUB_7742 PE=4 SV=1
MM1 pKa = 8.4SMIMAEE7 pKa = 4.25SFDD10 pKa = 4.95APMTDD15 pKa = 4.23SGDD18 pKa = 3.82TDD20 pKa = 3.26VSMYY24 pKa = 9.66PGGMTADD31 pKa = 3.31SWLSAEE37 pKa = 4.38ASMGDD42 pKa = 3.45VAYY45 pKa = 10.74SADD48 pKa = 3.74TATFDD53 pKa = 3.56YY54 pKa = 9.29MQEE57 pKa = 4.0GVEE60 pKa = 4.8VEE62 pKa = 4.31MLDD65 pKa = 5.78DD66 pKa = 5.42LDD68 pKa = 6.08DD69 pKa = 4.72DD70 pKa = 4.51AAITEE75 pKa = 4.43YY76 pKa = 11.32EE77 pKa = 4.19MADD80 pKa = 4.28DD81 pKa = 4.28GDD83 pKa = 3.88GYY85 pKa = 11.19YY86 pKa = 10.71DD87 pKa = 4.36HH88 pKa = 7.3EE89 pKa = 5.77LEE91 pKa = 4.42DD92 pKa = 4.24VEE94 pKa = 4.57VLDD97 pKa = 4.02VSRR100 pKa = 11.84GASIPPVGPADD111 pKa = 3.49VSVDD115 pKa = 3.39ANAGNSEE122 pKa = 4.14MMHH125 pKa = 5.64VPPYY129 pKa = 10.82DD130 pKa = 3.52GGAPVASGSHH140 pKa = 5.33YY141 pKa = 10.74SVEE144 pKa = 4.01PSRR147 pKa = 11.84DD148 pKa = 3.27DD149 pKa = 3.27SALYY153 pKa = 10.8SEE155 pKa = 4.79THH157 pKa = 5.12STAHH161 pKa = 6.95PEE163 pKa = 4.23HH164 pKa = 6.85PVEE167 pKa = 5.31ADD169 pKa = 3.13ALAEE173 pKa = 4.03TSAPLEE179 pKa = 4.36GDD181 pKa = 3.35VHH183 pKa = 6.61HH184 pKa = 7.01AVSASSPSAPDD195 pKa = 3.01VSAVVEE201 pKa = 4.54DD202 pKa = 3.91NGAPAEE208 pKa = 4.24LPAFPTLNHH217 pKa = 6.88AEE219 pKa = 4.22DD220 pKa = 4.71ASTSTDD226 pKa = 3.29PAASEE231 pKa = 4.18SHH233 pKa = 6.27SVSEE237 pKa = 5.5DD238 pKa = 3.24RR239 pKa = 11.84LLDD242 pKa = 3.75PVLAGPSDD250 pKa = 3.87VDD252 pKa = 3.36HH253 pKa = 7.1TSPVVPVDD261 pKa = 3.95SADD264 pKa = 3.52LHH266 pKa = 6.36PGEE269 pKa = 4.78EE270 pKa = 4.56SAHH273 pKa = 5.96EE274 pKa = 3.94LAADD278 pKa = 4.19DD279 pKa = 3.99PHH281 pKa = 6.85EE282 pKa = 4.69ISDD285 pKa = 4.13GVYY288 pKa = 9.65IDD290 pKa = 4.54PPPPVLLSLPPSAEE304 pKa = 3.75HH305 pKa = 7.53AEE307 pKa = 4.15YY308 pKa = 11.07CLFNQPQSSTSPSTPGGSKK327 pKa = 10.06SEE329 pKa = 4.2PAAEE333 pKa = 4.04PLRR336 pKa = 11.84LLLHH340 pKa = 6.8DD341 pKa = 5.16RR342 pKa = 11.84PTLYY346 pKa = 10.87YY347 pKa = 9.97EE348 pKa = 4.35PLSSVFDD355 pKa = 3.96ALRR358 pKa = 11.84HH359 pKa = 5.18EE360 pKa = 4.41EE361 pKa = 4.81CILNLPGSSDD371 pKa = 3.61AEE373 pKa = 4.27LVLDD377 pKa = 4.72AYY379 pKa = 8.98EE380 pKa = 4.49LRR382 pKa = 11.84LAICEE387 pKa = 4.26DD388 pKa = 3.15NVYY391 pKa = 10.55AHH393 pKa = 6.92EE394 pKa = 4.45VTLHH398 pKa = 5.75EE399 pKa = 5.24LDD401 pKa = 4.53VIHH404 pKa = 6.95GGSDD408 pKa = 2.92LHH410 pKa = 7.38GPLRR414 pKa = 11.84LKK416 pKa = 10.86LKK418 pKa = 10.03FVAPRR423 pKa = 11.84FVTRR427 pKa = 11.84YY428 pKa = 9.24HH429 pKa = 7.38LLRR432 pKa = 11.84EE433 pKa = 4.23QISRR437 pKa = 11.84LNLTADD443 pKa = 3.51GDD445 pKa = 4.14EE446 pKa = 4.54LLSGVAASDD455 pKa = 3.34PVYY458 pKa = 9.9EE459 pKa = 3.95QEE461 pKa = 3.99YY462 pKa = 10.26DD463 pKa = 3.47VSHH466 pKa = 6.19EE467 pKa = 4.07AEE469 pKa = 4.64VPHH472 pKa = 6.26HH473 pKa = 6.11HH474 pKa = 7.18AEE476 pKa = 4.35DD477 pKa = 4.34GEE479 pKa = 4.2ARR481 pKa = 11.84ASVDD485 pKa = 3.09EE486 pKa = 5.47HH487 pKa = 7.7YY488 pKa = 10.9DD489 pKa = 3.56GPIEE493 pKa = 4.1GTEE496 pKa = 4.2DD497 pKa = 3.39GTHH500 pKa = 5.89EE501 pKa = 4.18EE502 pKa = 4.09LRR504 pKa = 11.84EE505 pKa = 4.08GVDD508 pKa = 3.21ASEE511 pKa = 4.99AEE513 pKa = 4.14ALAPNAGLTDD523 pKa = 3.21NHH525 pKa = 6.2VASEE529 pKa = 4.01RR530 pKa = 11.84HH531 pKa = 4.92EE532 pKa = 4.32AVPEE536 pKa = 3.93QLDD539 pKa = 4.24GEE541 pKa = 4.94DD542 pKa = 3.17GTAALEE548 pKa = 4.76GPSRR552 pKa = 11.84EE553 pKa = 4.4ATTAPPDD560 pKa = 3.37VFEE563 pKa = 5.3AEE565 pKa = 4.3RR566 pKa = 11.84ADD568 pKa = 3.92LEE570 pKa = 4.6HH571 pKa = 6.69EE572 pKa = 4.43HH573 pKa = 5.94EE574 pKa = 4.28QEE576 pKa = 4.53DD577 pKa = 3.92VGEE580 pKa = 4.68GEE582 pKa = 4.47GTLDD586 pKa = 3.49ANDD589 pKa = 3.57VHH591 pKa = 6.21EE592 pKa = 4.99HH593 pKa = 6.42ADD595 pKa = 3.3AGEE598 pKa = 4.31GGDD601 pKa = 3.75YY602 pKa = 10.92VEE604 pKa = 5.7HH605 pKa = 7.93DD606 pKa = 4.59EE607 pKa = 5.88FPDD610 pKa = 5.07DD611 pKa = 4.64EE612 pKa = 6.92DD613 pKa = 6.23DD614 pKa = 5.63FGDD617 pKa = 4.96DD618 pKa = 3.72LPEE621 pKa = 4.69EE622 pKa = 4.32IGGQTEE628 pKa = 4.13NEE630 pKa = 4.57AYY632 pKa = 8.05THH634 pKa = 6.35TGAVEE639 pKa = 4.17DD640 pKa = 4.69APQDD644 pKa = 3.27SAYY647 pKa = 10.6AEE649 pKa = 4.23EE650 pKa = 4.69EE651 pKa = 4.27GVDD654 pKa = 3.43AHH656 pKa = 7.09EE657 pKa = 5.31DD658 pKa = 3.41ALEE661 pKa = 4.25ASSGDD666 pKa = 3.72AGQGDD671 pKa = 4.54PDD673 pKa = 4.15AASLAQDD680 pKa = 4.32DD681 pKa = 4.9EE682 pKa = 5.72GPLADD687 pKa = 4.95GLDD690 pKa = 4.34TNGHH694 pKa = 5.96TDD696 pKa = 3.27TFAHH700 pKa = 7.03LDD702 pKa = 3.51SADD705 pKa = 4.57DD706 pKa = 3.75YY707 pKa = 12.03DD708 pKa = 4.27EE709 pKa = 5.94LHH711 pKa = 6.5LQDD714 pKa = 4.64VGTGQHH720 pKa = 6.87DD721 pKa = 4.38DD722 pKa = 3.7EE723 pKa = 4.88STAAHH728 pKa = 6.73PVTNEE733 pKa = 3.15HH734 pKa = 5.58SQNYY738 pKa = 9.43EE739 pKa = 3.84DD740 pKa = 5.92DD741 pKa = 4.16PLTNEE746 pKa = 4.0EE747 pKa = 4.47STTLSSEE754 pKa = 4.04EE755 pKa = 5.11DD756 pKa = 3.56GLADD760 pKa = 5.69DD761 pKa = 5.21WDD763 pKa = 5.26DD764 pKa = 5.28DD765 pKa = 5.58DD766 pKa = 6.82DD767 pKa = 4.65VLDD770 pKa = 3.98VDD772 pKa = 5.13AVPAPEE778 pKa = 4.44VNQADD783 pKa = 3.77ALSRR787 pKa = 11.84KK788 pKa = 9.57SSSATLASKK797 pKa = 10.4SSKK800 pKa = 8.78RR801 pKa = 11.84TYY803 pKa = 11.19DD804 pKa = 3.38EE805 pKa = 4.94VEE807 pKa = 4.54LDD809 pKa = 5.51DD810 pKa = 6.38FDD812 pKa = 6.89DD813 pKa = 6.13DD814 pKa = 4.55PSQLEE819 pKa = 4.19AASTPAEE826 pKa = 4.55DD827 pKa = 4.37VPSGPPQQ834 pKa = 3.39

Molecular weight: 88.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M2W7U4|A0A1M2W7U4_TRAPU Uncharacterized protein OS=Trametes pubescens OX=154538 GN=TRAPUB_14140 PE=4 SV=1
AA1 pKa = 7.18TPAHH5 pKa = 6.54ASLRR9 pKa = 11.84PPNPRR14 pKa = 11.84LRR16 pKa = 11.84NRR18 pKa = 11.84ATQPRR23 pKa = 11.84PPAPSVPRR31 pKa = 11.84PAPRR35 pKa = 11.84APHH38 pKa = 5.24LHH40 pKa = 5.78RR41 pKa = 11.84RR42 pKa = 11.84ARR44 pKa = 11.84PHH46 pKa = 6.09VSHH49 pKa = 6.94SNRR52 pKa = 11.84AIRR55 pKa = 11.84LRR57 pKa = 11.84RR58 pKa = 11.84AAPP61 pKa = 3.36

Molecular weight: 6.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14147

0

14147

6007546

49

5047

424.7

46.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.985 ± 0.023

1.19 ± 0.009

5.779 ± 0.015

5.995 ± 0.02

3.474 ± 0.012

6.648 ± 0.021

2.6 ± 0.01

4.182 ± 0.013

4.106 ± 0.019

9.071 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.037 ± 0.008

2.94 ± 0.01

7.06 ± 0.029

3.645 ± 0.011

6.673 ± 0.018

8.144 ± 0.03

5.95 ± 0.014

6.522 ± 0.017

1.451 ± 0.008

2.546 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski