Tomato leaf curl Bangladesh virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9QDE5|Q9QDE5_9GEMI Transcriptional activator protein OS=Tomato leaf curl Bangladesh virus OX=223344 GN=C2 PE=3 SV=1
MM1 pKa = 7.36PQPKK5 pKa = 9.81RR6 pKa = 11.84FQIYY10 pKa = 9.63AKK12 pKa = 10.27NYY14 pKa = 8.01FLTYY18 pKa = 8.4PQCSLTKK25 pKa = 10.54EE26 pKa = 4.05EE27 pKa = 5.03ALSQIKK33 pKa = 10.27NLATPTNKK41 pKa = 10.09LFIRR45 pKa = 11.84VSRR48 pKa = 11.84EE49 pKa = 3.33LHH51 pKa = 6.28EE52 pKa = 4.8NGEE55 pKa = 4.28PHH57 pKa = 6.66LHH59 pKa = 6.03VLIQFEE65 pKa = 5.24GKK67 pKa = 10.07FKK69 pKa = 10.55CQNNRR74 pKa = 11.84FFDD77 pKa = 4.02LVSTTRR83 pKa = 11.84SANFHH88 pKa = 6.73PNIQGAKK95 pKa = 8.99SASDD99 pKa = 3.1VKK101 pKa = 11.14AYY103 pKa = 10.12VEE105 pKa = 4.21KK106 pKa = 11.12DD107 pKa = 2.71GDD109 pKa = 4.11FIDD112 pKa = 5.11FGVFQIDD119 pKa = 3.2GRR121 pKa = 11.84SARR124 pKa = 11.84GGCQSANDD132 pKa = 4.26AYY134 pKa = 10.9AEE136 pKa = 4.72AINSGSKK143 pKa = 10.09AAALNILRR151 pKa = 11.84EE152 pKa = 4.1KK153 pKa = 10.62APKK156 pKa = 10.5DD157 pKa = 3.42FVLQFHH163 pKa = 6.7NLNANLDD170 pKa = 4.29RR171 pKa = 11.84IFTPPVEE178 pKa = 4.62VYY180 pKa = 10.29VSPFNSSSFDD190 pKa = 3.45QVPEE194 pKa = 3.98EE195 pKa = 4.13LEE197 pKa = 3.58EE198 pKa = 3.9WAAEE202 pKa = 4.15NVVSAAARR210 pKa = 11.84PLRR213 pKa = 11.84PISIVIEE220 pKa = 4.23GDD222 pKa = 3.2SRR224 pKa = 11.84TGKK227 pKa = 8.52TMWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 4.39LSPKK249 pKa = 10.02VYY251 pKa = 10.94NNEE254 pKa = 3.24AWFNIIDD261 pKa = 4.47DD262 pKa = 4.12VDD264 pKa = 3.5PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.7EE273 pKa = 4.46FMGAQRR279 pKa = 11.84DD280 pKa = 3.81WQSNTKK286 pKa = 9.6YY287 pKa = 10.57GKK289 pKa = 9.12PVQIKK294 pKa = 10.41GGIPTIFLCNPGPNSSYY311 pKa = 11.34KK312 pKa = 10.62EE313 pKa = 3.85FLDD316 pKa = 3.79EE317 pKa = 4.63EE318 pKa = 4.37KK319 pKa = 11.05NAALKK324 pKa = 10.45NWALKK329 pKa = 10.24NAIFVTLEE337 pKa = 3.64GALYY341 pKa = 10.65SGSNQSAAQDD351 pKa = 3.81SQEE354 pKa = 3.84GDD356 pKa = 3.36QASSCC361 pKa = 3.88

Molecular weight: 40.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9QDE4|Q9QDE4_9GEMI Replication-associated protein OS=Tomato leaf curl Bangladesh virus OX=223344 GN=C1 PE=3 SV=1
MM1 pKa = 7.59GNLIFTCSSSSKK13 pKa = 9.59EE14 pKa = 3.91NSSVRR19 pKa = 11.84TTDD22 pKa = 2.59SSTWFPQPGQQISIRR37 pKa = 11.84TFRR40 pKa = 11.84EE41 pKa = 3.22LRR43 pKa = 11.84ARR45 pKa = 11.84QMSKK49 pKa = 10.48PMWRR53 pKa = 11.84KK54 pKa = 7.78TEE56 pKa = 3.72TSLILEE62 pKa = 5.13FSRR65 pKa = 11.84SMADD69 pKa = 3.18QLEE72 pKa = 4.53EE73 pKa = 4.17VANLPTTHH81 pKa = 6.76MPRR84 pKa = 11.84QSIQGPRR91 pKa = 11.84LRR93 pKa = 11.84PSIFF97 pKa = 3.13

Molecular weight: 11.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1100

97

361

183.3

20.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.545 ± 1.043

2.182 ± 0.514

4.909 ± 0.426

4.818 ± 0.838

5.091 ± 0.486

4.545 ± 0.473

3.727 ± 0.924

5.273 ± 0.883

5.818 ± 0.552

6.364 ± 0.725

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.273 ± 0.667

5.545 ± 1.022

5.727 ± 0.529

5.455 ± 0.645

6.818 ± 1.21

8.273 ± 1.382

5.091 ± 0.773

6.636 ± 1.196

1.455 ± 0.149

3.455 ± 0.591
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski