Xanthomonas phage Xoo-sp13

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 401 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D0GNP0|A0A7D0GNP0_9CAUD Uncharacterized protein OS=Xanthomonas phage Xoo-sp13 OX=2591401 GN=Xoosp13_12 PE=4 SV=1
MM1 pKa = 7.39LPWDD5 pKa = 3.87AQYY8 pKa = 9.79DD9 pKa = 4.36TVGLDD14 pKa = 3.32TNSARR19 pKa = 11.84LQIFIDD25 pKa = 3.85NVDD28 pKa = 3.31STKK31 pKa = 10.62PYY33 pKa = 10.84DD34 pKa = 3.78LVYY37 pKa = 9.43SCPVIGPEE45 pKa = 4.06YY46 pKa = 10.42KK47 pKa = 10.8AMPLVMQLAIEE58 pKa = 3.91LLIRR62 pKa = 11.84KK63 pKa = 6.21TCNGSDD69 pKa = 3.65PEE71 pKa = 4.13

Molecular weight: 7.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D0KNL5|A0A7D0KNL5_9CAUD Dihydrofolate reductase OS=Xanthomonas phage Xoo-sp13 OX=2591401 GN=Xoosp13_185 PE=3 SV=1
MM1 pKa = 6.37STRR4 pKa = 11.84YY5 pKa = 9.28FVFSKK10 pKa = 10.77KK11 pKa = 10.36SFNRR15 pKa = 11.84TGTAKK20 pKa = 10.61ALKK23 pKa = 9.9NAATRR28 pKa = 11.84EE29 pKa = 3.96DD30 pKa = 3.37ARR32 pKa = 11.84DD33 pKa = 3.73FKK35 pKa = 11.19RR36 pKa = 11.84NQNSPTAYY44 pKa = 10.37GIMDD48 pKa = 4.51RR49 pKa = 11.84STGEE53 pKa = 3.93VIRR56 pKa = 4.88

Molecular weight: 6.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

401

0

401

84637

37

3245

211.1

23.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.806 ± 0.157

0.916 ± 0.053

6.329 ± 0.151

5.845 ± 0.21

4.175 ± 0.098

6.204 ± 0.14

1.886 ± 0.078

6.36 ± 0.113

6.042 ± 0.243

7.634 ± 0.171

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.434 ± 0.072

5.754 ± 0.159

4.474 ± 0.21

3.334 ± 0.097

4.473 ± 0.104

7.284 ± 0.168

7.532 ± 0.433

7.086 ± 0.13

1.373 ± 0.065

4.059 ± 0.112
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski