Enterococcus faecium (strain ATCC BAA-472 / TX0016 / DO)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Enterococcus; Enterococcus faecium

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3059 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q3Y2R2|Q3Y2R2_ENTFD Ribonuclease BN OS=Enterococcus faecium (strain ATCC BAA-472 / TX0016 / DO) OX=333849 GN=rbn PE=4 SV=1
MM1 pKa = 7.62ALAKK5 pKa = 9.78IVYY8 pKa = 10.47ASMTGNTEE16 pKa = 3.89EE17 pKa = 4.35IADD20 pKa = 3.64IVAEE24 pKa = 4.08AFEE27 pKa = 4.27NLDD30 pKa = 3.21IEE32 pKa = 4.81VEE34 pKa = 4.24INEE37 pKa = 4.46CTQVDD42 pKa = 3.28ADD44 pKa = 4.1EE45 pKa = 5.21FEE47 pKa = 4.27EE48 pKa = 5.84ADD50 pKa = 3.34ICVVATYY57 pKa = 9.21TYY59 pKa = 11.3DD60 pKa = 5.15DD61 pKa = 4.39GDD63 pKa = 4.23LPDD66 pKa = 5.21EE67 pKa = 4.42IVDD70 pKa = 4.47FYY72 pKa = 11.82EE73 pKa = 5.32DD74 pKa = 3.86LQEE77 pKa = 5.5LDD79 pKa = 4.68LLGKK83 pKa = 10.15IYY85 pKa = 9.94GVCGSGDD92 pKa = 3.21TFYY95 pKa = 11.45DD96 pKa = 4.02EE97 pKa = 4.62FCKK100 pKa = 10.96SVDD103 pKa = 3.69DD104 pKa = 4.61FDD106 pKa = 5.18AAFAKK111 pKa = 10.05TGASKK116 pKa = 10.56GAEE119 pKa = 3.85NVKK122 pKa = 10.19VDD124 pKa = 4.48LNAEE128 pKa = 4.09EE129 pKa = 4.29EE130 pKa = 4.49DD131 pKa = 3.88IEE133 pKa = 4.32NLEE136 pKa = 4.19AFAKK140 pKa = 10.42EE141 pKa = 3.93LVAKK145 pKa = 10.4II146 pKa = 4.05

Molecular weight: 16.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I3U5Q0|I3U5Q0_ENTFD Uncharacterized protein OS=Enterococcus faecium (strain ATCC BAA-472 / TX0016 / DO) OX=333849 GN=HMPREF0351_12714 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 9.16RR10 pKa = 11.84KK11 pKa = 9.12RR12 pKa = 11.84QKK14 pKa = 9.38VHH16 pKa = 5.8GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 9.46NGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.73GRR39 pKa = 11.84KK40 pKa = 8.76VLSAA44 pKa = 4.05

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3059

0

3059

857693

37

1452

280.4

31.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.73 ± 0.041

0.686 ± 0.013

5.06 ± 0.036

7.557 ± 0.057

4.777 ± 0.036

6.365 ± 0.046

1.898 ± 0.02

7.712 ± 0.046

7.197 ± 0.048

9.868 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.731 ± 0.023

4.546 ± 0.031

3.431 ± 0.025

4.2 ± 0.038

4.06 ± 0.035

6.02 ± 0.041

5.761 ± 0.032

6.573 ± 0.041

0.983 ± 0.016

3.846 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski