Citrus psorosis virus (isolate Spain/P-121) (CPsV) (Citrus ringspot virus)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Milneviricetes; Serpentovirales; Aspiviridae; Ophiovirus; Citrus psorosis ophiovirus

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q6DN67|L_CPSVP RNA-directed RNA polymerase L OS=Citrus psorosis virus (isolate Spain/P-121) OX=652963 GN=L PE=3 SV=1
MM1 pKa = 7.69AEE3 pKa = 4.06YY4 pKa = 10.47IEE6 pKa = 4.14VRR8 pKa = 11.84VEE10 pKa = 4.27NLHH13 pKa = 6.35KK14 pKa = 10.21WGLEE18 pKa = 3.48INMEE22 pKa = 4.47RR23 pKa = 11.84IEE25 pKa = 4.28KK26 pKa = 10.09LSKK29 pKa = 9.85RR30 pKa = 11.84LKK32 pKa = 10.66SIVDD36 pKa = 3.54EE37 pKa = 4.3DD38 pKa = 5.28CIMKK42 pKa = 9.32TSRR45 pKa = 11.84IIGIWMFMPEE55 pKa = 4.06IVQEE59 pKa = 4.21SLKK62 pKa = 10.76DD63 pKa = 3.79SPLMTQKK70 pKa = 11.06AWIIPHH76 pKa = 6.12EE77 pKa = 4.36KK78 pKa = 9.32TYY80 pKa = 9.21KK81 pKa = 9.68TIYY84 pKa = 10.14GKK86 pKa = 10.31DD87 pKa = 3.85GIQMAVTQNEE97 pKa = 3.65EE98 pKa = 4.37DD99 pKa = 3.62LFKK102 pKa = 11.07DD103 pKa = 3.46SEE105 pKa = 4.26FFMISRR111 pKa = 11.84CDD113 pKa = 3.46SVMLTKK119 pKa = 10.7NNKK122 pKa = 8.8TIILNKK128 pKa = 10.14EE129 pKa = 3.99LLNCNMSEE137 pKa = 4.27DD138 pKa = 3.8MLFNMLSCQEE148 pKa = 3.76QDD150 pKa = 2.85ITEE153 pKa = 4.04EE154 pKa = 3.99LMKK157 pKa = 10.7KK158 pKa = 8.73MKK160 pKa = 9.96TIISSNPKK168 pKa = 9.57EE169 pKa = 3.97RR170 pKa = 11.84LEE172 pKa = 4.6DD173 pKa = 3.34KK174 pKa = 10.6TEE176 pKa = 3.84EE177 pKa = 4.23VFWNSTRR184 pKa = 11.84ILNWIQHH191 pKa = 5.63NDD193 pKa = 3.09NSRR196 pKa = 11.84SNSSDD201 pKa = 2.86NSFRR205 pKa = 11.84EE206 pKa = 4.1

Molecular weight: 24.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q6DN65|VP54_CPSVP Uncharacterized 54 kDa protein OS=Citrus psorosis virus (isolate Spain/P-121) OX=652963 GN=54K PE=4 SV=1
MM1 pKa = 7.81SIPIKK6 pKa = 10.54VSQLIDD12 pKa = 3.72AKK14 pKa = 9.77TKK16 pKa = 9.6WEE18 pKa = 4.07SEE20 pKa = 4.29KK21 pKa = 10.71EE22 pKa = 3.93KK23 pKa = 11.15SPAEE27 pKa = 3.77ILTLLKK33 pKa = 10.05TYY35 pKa = 10.58GVLRR39 pKa = 11.84DD40 pKa = 3.71TGALSSKK47 pKa = 9.24STAFLSGLSKK57 pKa = 10.81DD58 pKa = 3.48KK59 pKa = 10.97RR60 pKa = 11.84VILTDD65 pKa = 3.1EE66 pKa = 4.37GLAEE70 pKa = 4.47FKK72 pKa = 10.84SVEE75 pKa = 4.09NPHH78 pKa = 6.81DD79 pKa = 3.78QGEE82 pKa = 4.54KK83 pKa = 9.89PAFTLASSSSIKK95 pKa = 8.8ITNIEE100 pKa = 4.12VIKK103 pKa = 10.76QKK105 pKa = 10.04MEE107 pKa = 3.8QEE109 pKa = 4.4TFQIDD114 pKa = 3.56QLKK117 pKa = 10.62LKK119 pKa = 9.79EE120 pKa = 4.02QIEE123 pKa = 4.34NFIKK127 pKa = 10.02TVTLDD132 pKa = 3.54DD133 pKa = 3.75EE134 pKa = 5.54SYY136 pKa = 10.45TEE138 pKa = 4.08GEE140 pKa = 4.09IIIKK144 pKa = 10.52HH145 pKa = 6.1FGNPDD150 pKa = 3.1AEE152 pKa = 4.45LNMLITAGTKK162 pKa = 9.7ILDD165 pKa = 3.61GLVYY169 pKa = 10.93VSMKK173 pKa = 10.87GDD175 pKa = 3.55TKK177 pKa = 11.19SLNLFKK183 pKa = 10.21MEE185 pKa = 4.27QVDD188 pKa = 4.77GVCDD192 pKa = 3.55SDD194 pKa = 3.61IIKK197 pKa = 10.44NIRR200 pKa = 11.84VAKK203 pKa = 10.07RR204 pKa = 11.84AIQAAFVLIFTQGSLPGKK222 pKa = 10.24ADD224 pKa = 4.07DD225 pKa = 3.96KK226 pKa = 11.21RR227 pKa = 11.84KK228 pKa = 10.14VPEE231 pKa = 3.91FVKK234 pKa = 10.82SKK236 pKa = 10.76LYY238 pKa = 11.05DD239 pKa = 3.77GDD241 pKa = 3.79VSLSQISEE249 pKa = 4.34EE250 pKa = 4.38LSHH253 pKa = 6.94APTKK257 pKa = 10.44KK258 pKa = 9.68FPARR262 pKa = 11.84VFLKK266 pKa = 10.2IDD268 pKa = 3.38IDD270 pKa = 3.93NLPSAVCSRR279 pKa = 11.84CKK281 pKa = 10.78LNIAGNRR288 pKa = 11.84SVRR291 pKa = 11.84YY292 pKa = 9.72AGFASSFQTKK302 pKa = 9.28QKK304 pKa = 10.61LSPAVGATPEE314 pKa = 4.09SLMPLLEE321 pKa = 4.29TNQKK325 pKa = 9.31IEE327 pKa = 3.85KK328 pKa = 9.86SIAIRR333 pKa = 11.84DD334 pKa = 3.87FLKK337 pKa = 10.4TMEE340 pKa = 4.63GQWKK344 pKa = 7.65NQKK347 pKa = 9.56RR348 pKa = 11.84LHH350 pKa = 6.31PLSDD354 pKa = 3.68EE355 pKa = 4.28KK356 pKa = 10.79PTIKK360 pKa = 10.7NFTLKK365 pKa = 9.16LTCAIIYY372 pKa = 10.26SLTPDD377 pKa = 3.3GRR379 pKa = 11.84IDD381 pKa = 3.25MAEE384 pKa = 4.19RR385 pKa = 11.84IITDD389 pKa = 3.52KK390 pKa = 11.58NKK392 pKa = 10.24GFQNDD397 pKa = 3.15RR398 pKa = 11.84NFFGDD403 pKa = 3.64GEE405 pKa = 4.97GPTRR409 pKa = 11.84TWSVLTKK416 pKa = 10.45PEE418 pKa = 4.31ADD420 pKa = 3.62FSNITVDD427 pKa = 3.37GLKK430 pKa = 10.84GIFGVASTMM439 pKa = 3.81

Molecular weight: 48.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3537

206

2416

884.3

101.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.76 ± 0.582

1.159 ± 0.15

5.966 ± 0.38

9.019 ± 0.56

4.015 ± 0.206

4.891 ± 0.438

1.414 ± 0.173

9.358 ± 0.695

9.867 ± 0.18

9.075 ± 0.512

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.902 ± 0.473

5.909 ± 0.581

2.29 ± 0.458

2.771 ± 0.366

4.467 ± 0.243

7.888 ± 0.599

5.146 ± 0.462

4.891 ± 0.399

0.876 ± 0.197

3.308 ± 0.86
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski