Nitrososphaeraceae archaeon

Taxonomy: cellular organisms; Archaea; TACK group; Thaumarchaeota; Nitrososphaeria; Nitrososphaerales; Nitrososphaeraceae; unclassified Nitrososphaeraceae

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3773 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6A7LEI3|A0A6A7LEI3_9ARCH Winged helix-turn-helix transcriptional regulator OS=Nitrososphaeraceae archaeon OX=2169593 GN=GEU26_06255 PE=4 SV=1
MM1 pKa = 7.98DD2 pKa = 4.84NKK4 pKa = 10.85SFLISVLGLLLVMVYY19 pKa = 8.81FNPFGLHH26 pKa = 5.96DD27 pKa = 3.88QNVFGSVSDD36 pKa = 5.0DD37 pKa = 3.37EE38 pKa = 5.51DD39 pKa = 4.3NSVTEE44 pKa = 4.5QPLQQPLVNNSTSGIATSIYY64 pKa = 8.39DD65 pKa = 3.36THH67 pKa = 6.49EE68 pKa = 4.32FNVGDD73 pKa = 3.78GAQNLFILIPNEE85 pKa = 3.84GHH87 pKa = 6.65HH88 pKa = 6.76GPGEE92 pKa = 4.01EE93 pKa = 5.04DD94 pKa = 3.09EE95 pKa = 5.28ARR97 pKa = 11.84YY98 pKa = 10.0LNQPFVPEE106 pKa = 4.46SVTISPGTNVVWFNGDD122 pKa = 2.83VGHH125 pKa = 6.15EE126 pKa = 4.18HH127 pKa = 6.69NLAISGGGGSSNPLYY142 pKa = 9.59QTGEE146 pKa = 3.76FSEE149 pKa = 4.51FDD151 pKa = 3.12ARR153 pKa = 11.84NYY155 pKa = 8.45TFNEE159 pKa = 3.84IGDD162 pKa = 3.84FSYY165 pKa = 11.77ADD167 pKa = 3.33TVEE170 pKa = 4.17YY171 pKa = 10.85EE172 pKa = 3.8NGFIMRR178 pKa = 11.84GNISVTDD185 pKa = 4.76DD186 pKa = 4.64DD187 pKa = 5.12PSSQQGTTGSNPPTIGLLMVPTEE210 pKa = 5.12DD211 pKa = 4.87LAQHH215 pKa = 5.43TQDD218 pKa = 3.2LQGRR222 pKa = 11.84GFVIDD227 pKa = 3.73STHH230 pKa = 6.18NFPDD234 pKa = 3.41LRR236 pKa = 11.84EE237 pKa = 4.19GDD239 pKa = 3.93EE240 pKa = 3.95QTLVVWEE247 pKa = 4.39APEE250 pKa = 4.34NSDD253 pKa = 4.17ASTIIADD260 pKa = 3.99LAEE263 pKa = 4.37VSQQFPYY270 pKa = 10.86SS271 pKa = 3.42

Molecular weight: 29.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6A7LM11|A0A6A7LM11_9ARCH Lactamase_B domain-containing protein OS=Nitrososphaeraceae archaeon OX=2169593 GN=GEU26_17090 PE=4 SV=1
MM1 pKa = 7.26LHH3 pKa = 6.72SPLLLLPLPLLRR15 pKa = 11.84PLLLQLLPLLLRR27 pKa = 11.84LLLQLLLLMLRR38 pKa = 11.84PTRR41 pKa = 11.84LLLLLKK47 pKa = 10.48LLQLLLLLLRR57 pKa = 11.84LRR59 pKa = 11.84LLLRR63 pKa = 11.84LLLHH67 pKa = 6.55LLLRR71 pKa = 11.84RR72 pKa = 11.84PLRR75 pKa = 11.84LHH77 pKa = 5.89QLQLLLRR84 pKa = 11.84LLRR87 pKa = 11.84LHH89 pKa = 7.07LKK91 pKa = 10.15LLPLLRR97 pKa = 11.84LNYY100 pKa = 10.11LRR102 pKa = 11.84LRR104 pKa = 11.84LLLPLLLHH112 pKa = 6.74LLPLLLLQIRR122 pKa = 11.84LLLPLLPLLHH132 pKa = 7.31RR133 pKa = 11.84LRR135 pKa = 11.84PLLPLQIRR143 pKa = 11.84QLLPLLPLHH152 pKa = 6.72CLLLLLRR159 pKa = 11.84LLTLHH164 pKa = 6.39QLLPLLPPQSNLMPPPRR181 pKa = 11.84QLLLRR186 pKa = 4.45

Molecular weight: 22.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3773

0

3773

824061

20

1500

218.4

24.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.164 ± 0.043

1.082 ± 0.017

5.765 ± 0.031

6.414 ± 0.038

3.956 ± 0.026

6.545 ± 0.041

1.951 ± 0.02

8.332 ± 0.036

6.397 ± 0.041

9.111 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.553 ± 0.019

5.238 ± 0.035

3.826 ± 0.028

3.278 ± 0.023

5.109 ± 0.037

7.873 ± 0.04

5.509 ± 0.035

6.505 ± 0.034

0.93 ± 0.015

3.46 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski