Amino acid dipepetide frequency for Streptomyces sp. CB03234

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.086AlaAla: 22.086 ± 0.155
1.098AlaCys: 1.098 ± 0.024
8.199AlaAsp: 8.199 ± 0.067
8.871AlaGlu: 8.871 ± 0.103
3.577AlaPhe: 3.577 ± 0.047
13.362AlaGly: 13.362 ± 0.092
3.016AlaHis: 3.016 ± 0.043
3.549AlaIle: 3.549 ± 0.043
2.969AlaLys: 2.969 ± 0.05
14.738AlaLeu: 14.738 ± 0.117
2.632AlaMet: 2.632 ± 0.04
1.894AlaAsn: 1.894 ± 0.033
7.373AlaPro: 7.373 ± 0.092
3.615AlaGln: 3.615 ± 0.041
10.574AlaArg: 10.574 ± 0.088
5.692AlaSer: 5.692 ± 0.064
7.132AlaThr: 7.132 ± 0.067
12.499AlaVal: 12.499 ± 0.084
1.943AlaTrp: 1.943 ± 0.034
3.05AlaTyr: 3.05 ± 0.035
0.0AlaXaa: 0.0 ± 0.0
Cys
1.126CysAla: 1.126 ± 0.024
0.095CysCys: 0.095 ± 0.007
0.493CysAsp: 0.493 ± 0.016
0.423CysGlu: 0.423 ± 0.015
0.23CysPhe: 0.23 ± 0.011
0.992CysGly: 0.992 ± 0.024
0.198CysHis: 0.198 ± 0.01
0.172CysIle: 0.172 ± 0.009
0.107CysLys: 0.107 ± 0.007
0.759CysLeu: 0.759 ± 0.021
0.126CysMet: 0.126 ± 0.008
0.138CysAsn: 0.138 ± 0.008
0.503CysPro: 0.503 ± 0.017
0.149CysGln: 0.149 ± 0.009
0.627CysArg: 0.627 ± 0.017
0.409CysSer: 0.409 ± 0.013
0.508CysThr: 0.508 ± 0.014
0.724CysVal: 0.724 ± 0.02
0.126CysTrp: 0.126 ± 0.008
0.15CysTyr: 0.15 ± 0.007
0.0CysXaa: 0.0 ± 0.0
Asp
7.796AspAla: 7.796 ± 0.079
0.437AspCys: 0.437 ± 0.016
3.499AspAsp: 3.499 ± 0.049
3.943AspGlu: 3.943 ± 0.056
1.627AspPhe: 1.627 ± 0.025
6.317AspGly: 6.317 ± 0.062
1.426AspHis: 1.426 ± 0.028
1.96AspIle: 1.96 ± 0.032
1.245AspLys: 1.245 ± 0.028
6.016AspLeu: 6.016 ± 0.064
0.832AspMet: 0.832 ± 0.02
0.989AspAsn: 0.989 ± 0.023
4.468AspPro: 4.468 ± 0.051
1.521AspGln: 1.521 ± 0.031
4.973AspArg: 4.973 ± 0.056
2.274AspSer: 2.274 ± 0.033
3.227AspThr: 3.227 ± 0.04
4.812AspVal: 4.812 ± 0.054
1.001AspTrp: 1.001 ± 0.022
1.051AspTyr: 1.051 ± 0.024
0.0AspXaa: 0.0 ± 0.0
Glu
7.676GluAla: 7.676 ± 0.095
0.4GluCys: 0.4 ± 0.013
2.889GluAsp: 2.889 ± 0.04
3.77GluGlu: 3.77 ± 0.062
1.358GluPhe: 1.358 ± 0.027
4.427GluGly: 4.427 ± 0.049
1.472GluHis: 1.472 ± 0.028
2.181GluIle: 2.181 ± 0.034
1.541GluLys: 1.541 ± 0.034
6.883GluLeu: 6.883 ± 0.079
0.884GluMet: 0.884 ± 0.024
1.005GluAsn: 1.005 ± 0.023
3.547GluPro: 3.547 ± 0.042
2.218GluGln: 2.218 ± 0.037
5.749GluArg: 5.749 ± 0.065
2.405GluSer: 2.405 ± 0.034
2.868GluThr: 2.868 ± 0.037
4.487GluVal: 4.487 ± 0.051
0.809GluTrp: 0.809 ± 0.02
1.108GluTyr: 1.108 ± 0.025
0.0GluXaa: 0.0 ± 0.0
Phe
3.619PheAla: 3.619 ± 0.045
0.27PheCys: 0.27 ± 0.011
1.861PheAsp: 1.861 ± 0.03
1.374PheGlu: 1.374 ± 0.024
0.893PhePhe: 0.893 ± 0.024
2.951PheGly: 2.951 ± 0.045
0.632PheHis: 0.632 ± 0.017
0.742PheIle: 0.742 ± 0.021
0.506PheLys: 0.506 ± 0.017
2.599PheLeu: 2.599 ± 0.041
0.443PheMet: 0.443 ± 0.016
0.558PheAsn: 0.558 ± 0.016
1.408PhePro: 1.408 ± 0.026
0.681PheGln: 0.681 ± 0.018
1.863PheArg: 1.863 ± 0.03
1.459PheSer: 1.459 ± 0.028
2.081PheThr: 2.081 ± 0.033
2.112PheVal: 2.112 ± 0.034
0.417PheTrp: 0.417 ± 0.015
0.56PheTyr: 0.56 ± 0.018
0.0PheXaa: 0.0 ± 0.0
Gly
11.372GlyAla: 11.372 ± 0.091
0.881GlyCys: 0.881 ± 0.02
5.269GlyAsp: 5.269 ± 0.066
5.161GlyGlu: 5.161 ± 0.05
2.906GlyPhe: 2.906 ± 0.045
9.042GlyGly: 9.042 ± 0.099
2.383GlyHis: 2.383 ± 0.035
3.442GlyIle: 3.442 ± 0.043
2.47GlyLys: 2.47 ± 0.038
9.442GlyLeu: 9.442 ± 0.08
1.989GlyMet: 1.989 ± 0.035
1.704GlyAsn: 1.704 ± 0.03
5.235GlyPro: 5.235 ± 0.067
2.564GlyGln: 2.564 ± 0.038
7.926GlyArg: 7.926 ± 0.064
5.007GlySer: 5.007 ± 0.053
6.487GlyThr: 6.487 ± 0.067
7.602GlyVal: 7.602 ± 0.063
1.717GlyTrp: 1.717 ± 0.034
2.297GlyTyr: 2.297 ± 0.034
0.0GlyXaa: 0.0 ± 0.0
His
2.799HisAla: 2.799 ± 0.039
0.22HisCys: 0.22 ± 0.009
1.319HisAsp: 1.319 ± 0.023
1.238HisGlu: 1.238 ± 0.024
0.646HisPhe: 0.646 ± 0.017
2.428HisGly: 2.428 ± 0.036
0.717HisHis: 0.717 ± 0.021
0.711HisIle: 0.711 ± 0.017
0.369HisLys: 0.369 ± 0.013
2.454HisLeu: 2.454 ± 0.036
0.352HisMet: 0.352 ± 0.014
0.376HisAsn: 0.376 ± 0.013
1.861HisPro: 1.861 ± 0.037
0.666HisGln: 0.666 ± 0.018
2.11HisArg: 2.11 ± 0.036
0.973HisSer: 0.973 ± 0.022
1.401HisThr: 1.401 ± 0.027
1.728HisVal: 1.728 ± 0.029
0.355HisTrp: 0.355 ± 0.014
0.471HisTyr: 0.471 ± 0.015
0.0HisXaa: 0.0 ± 0.0
Ile
4.802IleAla: 4.802 ± 0.054
0.314IleCys: 0.314 ± 0.012
2.243IleAsp: 2.243 ± 0.031
2.042IleGlu: 2.042 ± 0.035
0.677IlePhe: 0.677 ± 0.02
3.642IleGly: 3.642 ± 0.046
0.622IleHis: 0.622 ± 0.015
0.87IleIle: 0.87 ± 0.024
0.749IleLys: 0.749 ± 0.023
2.371IleLeu: 2.371 ± 0.039
0.438IleMet: 0.438 ± 0.016
0.716IleAsn: 0.716 ± 0.019
1.77IlePro: 1.77 ± 0.029
0.734IleGln: 0.734 ± 0.018
2.236IleArg: 2.236 ± 0.034
1.573IleSer: 1.573 ± 0.031
2.215IleThr: 2.215 ± 0.03
2.727IleVal: 2.727 ± 0.038
0.361IleTrp: 0.361 ± 0.015
0.535IleTyr: 0.535 ± 0.014
0.0IleXaa: 0.0 ± 0.0
Lys
3.133LysAla: 3.133 ± 0.049
0.112LysCys: 0.112 ± 0.008
1.439LysAsp: 1.439 ± 0.03
1.264LysGlu: 1.264 ± 0.029
0.445LysPhe: 0.445 ± 0.016
1.895LysGly: 1.895 ± 0.033
0.441LysHis: 0.441 ± 0.016
0.921LysIle: 0.921 ± 0.023
0.89LysLys: 0.89 ± 0.03
2.074LysLeu: 2.074 ± 0.034
0.391LysMet: 0.391 ± 0.014
0.566LysAsn: 0.566 ± 0.018
1.403LysPro: 1.403 ± 0.033
0.719LysGln: 0.719 ± 0.022
1.545LysArg: 1.545 ± 0.03
1.041LysSer: 1.041 ± 0.026
1.273LysThr: 1.273 ± 0.027
1.952LysVal: 1.952 ± 0.035
0.262LysTrp: 0.262 ± 0.013
0.458LysTyr: 0.458 ± 0.015
0.0LysXaa: 0.0 ± 0.0
Leu
15.205LeuAla: 15.205 ± 0.116
0.846LeuCys: 0.846 ± 0.021
6.745LeuAsp: 6.745 ± 0.067
4.783LeuGlu: 4.783 ± 0.052
2.624LeuPhe: 2.624 ± 0.046
9.301LeuGly: 9.301 ± 0.067
2.3LeuHis: 2.3 ± 0.039
3.326LeuIle: 3.326 ± 0.042
2.163LeuLys: 2.163 ± 0.037
11.394LeuLeu: 11.394 ± 0.103
1.705LeuMet: 1.705 ± 0.034
1.69LeuAsn: 1.69 ± 0.029
6.499LeuPro: 6.499 ± 0.063
2.121LeuGln: 2.121 ± 0.032
8.675LeuArg: 8.675 ± 0.08
5.073LeuSer: 5.073 ± 0.054
6.824LeuThr: 6.824 ± 0.069
8.921LeuVal: 8.921 ± 0.081
1.296LeuTrp: 1.296 ± 0.027
1.913LeuTyr: 1.913 ± 0.035
0.0LeuXaa: 0.0 ± 0.0
Met
2.411MetAla: 2.411 ± 0.036
0.145MetCys: 0.145 ± 0.009
0.921MetAsp: 0.921 ± 0.024
0.821MetGlu: 0.821 ± 0.021
0.455MetPhe: 0.455 ± 0.016
1.375MetGly: 1.375 ± 0.028
0.352MetHis: 0.352 ± 0.014
0.614MetIle: 0.614 ± 0.017
0.427MetLys: 0.427 ± 0.015
1.735MetLeu: 1.735 ± 0.032
0.292MetMet: 0.292 ± 0.012
0.444MetAsn: 0.444 ± 0.014
1.146MetPro: 1.146 ± 0.02
0.422MetGln: 0.422 ± 0.015
1.458MetArg: 1.458 ± 0.027
1.263MetSer: 1.263 ± 0.026
1.564MetThr: 1.564 ± 0.028
1.299MetVal: 1.299 ± 0.03
0.225MetTrp: 0.225 ± 0.011
0.33MetTyr: 0.33 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
2.182AsnAla: 2.182 ± 0.035
0.17AsnCys: 0.17 ± 0.008
0.974AsnAsp: 0.974 ± 0.022
0.814AsnGlu: 0.814 ± 0.019
0.466AsnPhe: 0.466 ± 0.015
1.827AsnGly: 1.827 ± 0.033
0.388AsnHis: 0.388 ± 0.013
0.637AsnIle: 0.637 ± 0.02
0.461AsnLys: 0.461 ± 0.015
1.617AsnLeu: 1.617 ± 0.026
0.285AsnMet: 0.285 ± 0.012
0.423AsnAsn: 0.423 ± 0.018
1.34AsnPro: 1.34 ± 0.032
0.494AsnGln: 0.494 ± 0.017
1.235AsnArg: 1.235 ± 0.024
0.824AsnSer: 0.824 ± 0.019
1.068AsnThr: 1.068 ± 0.025
1.368AsnVal: 1.368 ± 0.025
0.279AsnTrp: 0.279 ± 0.012
0.389AsnTyr: 0.389 ± 0.014
0.0AsnXaa: 0.0 ± 0.0
Pro
8.988ProAla: 8.988 ± 0.088
0.35ProCys: 0.35 ± 0.013
4.462ProAsp: 4.462 ± 0.052
4.43ProGlu: 4.43 ± 0.053
1.531ProPhe: 1.531 ± 0.026
6.87ProGly: 6.87 ± 0.076
1.424ProHis: 1.424 ± 0.03
1.233ProIle: 1.233 ± 0.027
1.263ProLys: 1.263 ± 0.029
5.544ProLeu: 5.544 ± 0.062
1.045ProMet: 1.045 ± 0.024
0.85ProAsn: 0.85 ± 0.021
3.712ProPro: 3.712 ± 0.069
1.709ProGln: 1.709 ± 0.034
3.967ProArg: 3.967 ± 0.044
3.042ProSer: 3.042 ± 0.04
3.127ProThr: 3.127 ± 0.047
5.696ProVal: 5.696 ± 0.055
0.912ProTrp: 0.912 ± 0.024
1.516ProTyr: 1.516 ± 0.029
0.0ProXaa: 0.0 ± 0.0
Gln
3.702GlnAla: 3.702 ± 0.045
0.178GlnCys: 0.178 ± 0.01
1.332GlnAsp: 1.332 ± 0.027
1.435GlnGlu: 1.435 ± 0.031
0.667GlnPhe: 0.667 ± 0.018
2.248GlnGly: 2.248 ± 0.035
0.653GlnHis: 0.653 ± 0.018
1.02GlnIle: 1.02 ± 0.022
0.604GlnLys: 0.604 ± 0.018
2.961GlnLeu: 2.961 ± 0.036
0.483GlnMet: 0.483 ± 0.018
0.46GlnAsn: 0.46 ± 0.017
1.7GlnPro: 1.7 ± 0.035
1.208GlnGln: 1.208 ± 0.031
2.324GlnArg: 2.324 ± 0.035
1.127GlnSer: 1.127 ± 0.023
1.228GlnThr: 1.228 ± 0.027
2.316GlnVal: 2.316 ± 0.038
0.455GlnTrp: 0.455 ± 0.015
0.611GlnTyr: 0.611 ± 0.019
0.0GlnXaa: 0.0 ± 0.0
Arg
10.187ArgAla: 10.187 ± 0.092
0.619ArgCys: 0.619 ± 0.019
4.435ArgAsp: 4.435 ± 0.045
4.82ArgGlu: 4.82 ± 0.053
2.341ArgPhe: 2.341 ± 0.035
5.894ArgGly: 5.894 ± 0.056
2.162ArgHis: 2.162 ± 0.037
3.237ArgIle: 3.237 ± 0.046
1.733ArgLys: 1.733 ± 0.037
9.019ArgLeu: 9.019 ± 0.088
1.781ArgMet: 1.781 ± 0.029
1.313ArgAsn: 1.313 ± 0.027
5.078ArgPro: 5.078 ± 0.064
2.253ArgGln: 2.253 ± 0.037
7.775ArgArg: 7.775 ± 0.082
3.808ArgSer: 3.808 ± 0.04
5.55ArgThr: 5.55 ± 0.052
5.892ArgVal: 5.892 ± 0.056
1.377ArgTrp: 1.377 ± 0.027
1.868ArgTyr: 1.868 ± 0.03
0.0ArgXaa: 0.0 ± 0.0
Ser
6.254SerAla: 6.254 ± 0.056
0.391SerCys: 0.391 ± 0.014
2.462SerAsp: 2.462 ± 0.037
2.279SerGlu: 2.279 ± 0.036
1.455SerPhe: 1.455 ± 0.025
5.587SerGly: 5.587 ± 0.057
0.969SerHis: 0.969 ± 0.022
1.351SerIle: 1.351 ± 0.028
0.989SerLys: 0.989 ± 0.023
4.59SerLeu: 4.59 ± 0.049
1.041SerMet: 1.041 ± 0.021
0.787SerAsn: 0.787 ± 0.021
3.065SerPro: 3.065 ± 0.042
1.132SerGln: 1.132 ± 0.025
3.607SerArg: 3.607 ± 0.043
2.497SerSer: 2.497 ± 0.039
2.866SerThr: 2.866 ± 0.04
4.075SerVal: 4.075 ± 0.048
0.841SerTrp: 0.841 ± 0.019
1.17SerTyr: 1.17 ± 0.025
0.0SerXaa: 0.0 ± 0.0
Thr
8.86ThrAla: 8.86 ± 0.072
0.419ThrCys: 0.419 ± 0.015
3.648ThrAsp: 3.648 ± 0.044
3.295ThrGlu: 3.295 ± 0.042
1.607ThrPhe: 1.607 ± 0.033
6.859ThrGly: 6.859 ± 0.063
1.235ThrHis: 1.235 ± 0.026
1.768ThrIle: 1.768 ± 0.033
1.177ThrLys: 1.177 ± 0.026
5.727ThrLeu: 5.727 ± 0.055
0.981ThrMet: 0.981 ± 0.021
0.94ThrAsn: 0.94 ± 0.023
4.194ThrPro: 4.194 ± 0.057
1.242ThrGln: 1.242 ± 0.026
3.912ThrArg: 3.912 ± 0.041
3.014ThrSer: 3.014 ± 0.042
3.87ThrThr: 3.87 ± 0.057
6.216ThrVal: 6.216 ± 0.056
0.905ThrTrp: 0.905 ± 0.019
1.399ThrTyr: 1.399 ± 0.027
0.0ThrXaa: 0.0 ± 0.0
Val
10.946ValAla: 10.946 ± 0.084
0.757ValCys: 0.757 ± 0.019
4.898ValAsp: 4.898 ± 0.056
4.722ValGlu: 4.722 ± 0.053
2.369ValPhe: 2.369 ± 0.038
6.397ValGly: 6.397 ± 0.071
2.037ValHis: 2.037 ± 0.033
2.865ValIle: 2.865 ± 0.043
1.766ValLys: 1.766 ± 0.03
9.646ValLeu: 9.646 ± 0.073
1.459ValMet: 1.459 ± 0.027
1.593ValAsn: 1.593 ± 0.029
5.525ValPro: 5.525 ± 0.058
2.075ValGln: 2.075 ± 0.029
7.37ValArg: 7.37 ± 0.075
4.174ValSer: 4.174 ± 0.044
5.785ValThr: 5.785 ± 0.059
7.922ValVal: 7.922 ± 0.064
1.166ValTrp: 1.166 ± 0.024
1.599ValTyr: 1.599 ± 0.029
0.0ValXaa: 0.0 ± 0.0
Trp
1.725TrpAla: 1.725 ± 0.029
0.165TrpCys: 0.165 ± 0.008
0.815TrpAsp: 0.815 ± 0.02
0.741TrpGlu: 0.741 ± 0.017
0.513TrpPhe: 0.513 ± 0.017
1.06TrpGly: 1.06 ± 0.024
0.362TrpHis: 0.362 ± 0.013
0.536TrpIle: 0.536 ± 0.019
0.369TrpLys: 0.369 ± 0.013
1.765TrpLeu: 1.765 ± 0.033
0.28TrpMet: 0.28 ± 0.012
0.41TrpAsn: 0.41 ± 0.016
0.779TrpPro: 0.779 ± 0.019
0.612TrpGln: 0.612 ± 0.017
1.359TrpArg: 1.359 ± 0.023
0.875TrpSer: 0.875 ± 0.018
1.04TrpThr: 1.04 ± 0.025
0.998TrpVal: 0.998 ± 0.02
0.336TrpTrp: 0.336 ± 0.013
0.365TrpTyr: 0.365 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.999TyrAla: 2.999 ± 0.037
0.186TyrCys: 0.186 ± 0.01
1.511TyrAsp: 1.511 ± 0.028
1.452TyrGlu: 1.452 ± 0.024
0.641TyrPhe: 0.641 ± 0.018
2.421TyrGly: 2.421 ± 0.038
0.404TyrHis: 0.404 ± 0.014
0.502TyrIle: 0.502 ± 0.017
0.427TyrLys: 0.427 ± 0.016
2.07TyrLeu: 2.07 ± 0.035
0.258TyrMet: 0.258 ± 0.011
0.387TyrAsn: 0.387 ± 0.016
1.063TyrPro: 1.063 ± 0.022
0.574TyrGln: 0.574 ± 0.018
1.861TyrArg: 1.861 ± 0.027
0.877TyrSer: 0.877 ± 0.02
1.153TyrThr: 1.153 ± 0.027
1.728TyrVal: 1.728 ± 0.03
0.324TyrTrp: 0.324 ± 0.014
0.442TyrTyr: 0.442 ± 0.016
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6567 proteins (2133522 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski