Amino acid dipepetide frequency for Escherichia phage vB_EcoS_IME542

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.588AlaAla: 7.588 ± 1.205
0.537AlaCys: 0.537 ± 0.213
4.139AlaAsp: 4.139 ± 0.547
5.289AlaGlu: 5.289 ± 0.63
2.989AlaPhe: 2.989 ± 0.471
5.519AlaGly: 5.519 ± 0.609
0.996AlaHis: 0.996 ± 0.305
6.362AlaIle: 6.362 ± 0.772
6.515AlaLys: 6.515 ± 1.15
7.358AlaLeu: 7.358 ± 1.098
2.606AlaMet: 2.606 ± 0.512
4.675AlaAsn: 4.675 ± 0.688
2.223AlaPro: 2.223 ± 0.433
3.219AlaGln: 3.219 ± 0.796
5.365AlaArg: 5.365 ± 0.684
4.905AlaSer: 4.905 ± 0.514
4.982AlaThr: 4.982 ± 0.663
5.825AlaVal: 5.825 ± 0.743
0.843AlaTrp: 0.843 ± 0.293
2.913AlaTyr: 2.913 ± 0.486
0.0AlaXaa: 0.0 ± 0.0
Cys
0.69CysAla: 0.69 ± 0.229
0.23CysCys: 0.23 ± 0.13
0.613CysAsp: 0.613 ± 0.211
0.69CysGlu: 0.69 ± 0.22
0.46CysPhe: 0.46 ± 0.212
1.226CysGly: 1.226 ± 0.318
0.23CysHis: 0.23 ± 0.125
0.537CysIle: 0.537 ± 0.221
1.15CysLys: 1.15 ± 0.289
0.92CysLeu: 0.92 ± 0.254
0.537CysMet: 0.537 ± 0.24
0.69CysAsn: 0.69 ± 0.202
0.23CysPro: 0.23 ± 0.119
0.153CysGln: 0.153 ± 0.111
0.766CysArg: 0.766 ± 0.262
1.15CysSer: 1.15 ± 0.306
0.69CysThr: 0.69 ± 0.23
0.537CysVal: 0.537 ± 0.228
0.307CysTrp: 0.307 ± 0.127
0.307CysTyr: 0.307 ± 0.144
0.0CysXaa: 0.0 ± 0.0
Asp
5.059AspAla: 5.059 ± 0.905
0.383AspCys: 0.383 ± 0.168
3.296AspAsp: 3.296 ± 0.592
3.986AspGlu: 3.986 ± 0.566
2.299AspPhe: 2.299 ± 0.402
7.051AspGly: 7.051 ± 0.714
1.303AspHis: 1.303 ± 0.314
3.756AspIle: 3.756 ± 0.484
3.909AspLys: 3.909 ± 0.534
4.062AspLeu: 4.062 ± 0.515
1.226AspMet: 1.226 ± 0.324
3.142AspAsn: 3.142 ± 0.454
1.303AspPro: 1.303 ± 0.325
1.073AspGln: 1.073 ± 0.287
1.84AspArg: 1.84 ± 0.424
3.526AspSer: 3.526 ± 0.636
3.449AspThr: 3.449 ± 0.629
3.986AspVal: 3.986 ± 0.515
0.92AspTrp: 0.92 ± 0.234
2.836AspTyr: 2.836 ± 0.479
0.0AspXaa: 0.0 ± 0.0
Glu
5.825GluAla: 5.825 ± 0.635
0.69GluCys: 0.69 ± 0.228
3.142GluAsp: 3.142 ± 0.466
4.522GluGlu: 4.522 ± 0.679
2.989GluPhe: 2.989 ± 0.493
3.372GluGly: 3.372 ± 0.48
0.766GluHis: 0.766 ± 0.316
5.978GluIle: 5.978 ± 0.565
3.679GluLys: 3.679 ± 0.577
5.442GluLeu: 5.442 ± 0.609
3.219GluMet: 3.219 ± 0.681
2.913GluAsn: 2.913 ± 0.484
1.533GluPro: 1.533 ± 0.28
2.683GluGln: 2.683 ± 0.69
2.913GluArg: 2.913 ± 0.572
4.216GluSer: 4.216 ± 0.542
3.526GluThr: 3.526 ± 0.463
5.289GluVal: 5.289 ± 0.622
0.537GluTrp: 0.537 ± 0.183
2.913GluTyr: 2.913 ± 0.446
0.0GluXaa: 0.0 ± 0.0
Phe
3.142PheAla: 3.142 ± 0.523
0.843PheCys: 0.843 ± 0.247
3.142PheAsp: 3.142 ± 0.421
3.142PheGlu: 3.142 ± 0.522
0.766PhePhe: 0.766 ± 0.217
3.526PheGly: 3.526 ± 0.514
0.69PheHis: 0.69 ± 0.253
2.223PheIle: 2.223 ± 0.329
2.529PheLys: 2.529 ± 0.488
1.686PheLeu: 1.686 ± 0.417
1.38PheMet: 1.38 ± 0.363
2.146PheAsn: 2.146 ± 0.406
1.38PhePro: 1.38 ± 0.314
1.686PheGln: 1.686 ± 0.335
2.069PheArg: 2.069 ± 0.441
2.759PheSer: 2.759 ± 0.457
2.376PheThr: 2.376 ± 0.328
2.376PheVal: 2.376 ± 0.411
0.383PheTrp: 0.383 ± 0.15
1.073PheTyr: 1.073 ± 0.226
0.0PheXaa: 0.0 ± 0.0
Gly
4.982GlyAla: 4.982 ± 0.631
1.533GlyCys: 1.533 ± 0.392
4.522GlyAsp: 4.522 ± 0.635
4.522GlyGlu: 4.522 ± 0.668
3.296GlyPhe: 3.296 ± 0.495
5.289GlyGly: 5.289 ± 1.067
0.843GlyHis: 0.843 ± 0.291
5.289GlyIle: 5.289 ± 0.534
6.208GlyLys: 6.208 ± 0.747
5.978GlyLeu: 5.978 ± 0.532
2.146GlyMet: 2.146 ± 0.492
3.296GlyAsn: 3.296 ± 0.621
0.69GlyPro: 0.69 ± 0.217
1.916GlyGln: 1.916 ± 0.305
3.296GlyArg: 3.296 ± 0.503
5.212GlySer: 5.212 ± 0.647
3.832GlyThr: 3.832 ± 0.566
6.285GlyVal: 6.285 ± 0.687
1.226GlyTrp: 1.226 ± 0.319
3.756GlyTyr: 3.756 ± 0.593
0.0GlyXaa: 0.0 ± 0.0
His
0.766HisAla: 0.766 ± 0.233
0.23HisCys: 0.23 ± 0.12
0.69HisAsp: 0.69 ± 0.237
0.996HisGlu: 0.996 ± 0.327
0.537HisPhe: 0.537 ± 0.225
1.456HisGly: 1.456 ± 0.392
0.613HisHis: 0.613 ± 0.231
1.763HisIle: 1.763 ± 0.447
1.533HisLys: 1.533 ± 0.301
1.303HisLeu: 1.303 ± 0.41
0.383HisMet: 0.383 ± 0.168
0.537HisAsn: 0.537 ± 0.228
0.383HisPro: 0.383 ± 0.234
0.46HisGln: 0.46 ± 0.155
1.073HisArg: 1.073 ± 0.308
0.69HisSer: 0.69 ± 0.217
0.537HisThr: 0.537 ± 0.178
1.073HisVal: 1.073 ± 0.299
0.077HisTrp: 0.077 ± 0.075
0.46HisTyr: 0.46 ± 0.189
0.0HisXaa: 0.0 ± 0.0
Ile
7.435IleAla: 7.435 ± 0.909
0.23IleCys: 0.23 ± 0.124
5.595IleAsp: 5.595 ± 0.726
4.062IleGlu: 4.062 ± 0.521
1.916IlePhe: 1.916 ± 0.308
4.829IleGly: 4.829 ± 0.66
0.843IleHis: 0.843 ± 0.271
3.526IleIle: 3.526 ± 0.482
5.059IleLys: 5.059 ± 0.644
3.066IleLeu: 3.066 ± 0.491
1.533IleMet: 1.533 ± 0.363
3.832IleAsn: 3.832 ± 0.606
3.066IlePro: 3.066 ± 0.504
2.299IleGln: 2.299 ± 0.564
2.759IleArg: 2.759 ± 0.375
4.752IleSer: 4.752 ± 0.555
4.599IleThr: 4.599 ± 0.529
4.445IleVal: 4.445 ± 0.583
0.69IleTrp: 0.69 ± 0.184
2.683IleTyr: 2.683 ± 0.508
0.0IleXaa: 0.0 ± 0.0
Lys
7.128LysAla: 7.128 ± 1.022
0.46LysCys: 0.46 ± 0.212
3.602LysAsp: 3.602 ± 0.422
5.365LysGlu: 5.365 ± 0.918
2.989LysPhe: 2.989 ± 0.415
3.449LysGly: 3.449 ± 0.433
0.92LysHis: 0.92 ± 0.278
4.062LysIle: 4.062 ± 0.451
4.292LysLys: 4.292 ± 0.568
5.059LysLeu: 5.059 ± 0.646
3.066LysMet: 3.066 ± 0.523
3.142LysAsn: 3.142 ± 0.515
1.993LysPro: 1.993 ± 0.404
2.299LysGln: 2.299 ± 0.42
3.372LysArg: 3.372 ± 0.596
4.216LysSer: 4.216 ± 0.598
3.449LysThr: 3.449 ± 0.514
5.672LysVal: 5.672 ± 0.751
0.69LysTrp: 0.69 ± 0.239
3.602LysTyr: 3.602 ± 0.548
0.0LysXaa: 0.0 ± 0.0
Leu
5.978LeuAla: 5.978 ± 0.892
1.15LeuCys: 1.15 ± 0.25
4.369LeuAsp: 4.369 ± 0.595
4.062LeuGlu: 4.062 ± 0.669
2.376LeuPhe: 2.376 ± 0.335
4.905LeuGly: 4.905 ± 0.692
0.843LeuHis: 0.843 ± 0.271
4.752LeuIle: 4.752 ± 0.492
3.296LeuLys: 3.296 ± 0.542
3.756LeuLeu: 3.756 ± 0.563
0.843LeuMet: 0.843 ± 0.258
2.989LeuAsn: 2.989 ± 0.426
2.683LeuPro: 2.683 ± 0.428
2.683LeuGln: 2.683 ± 0.908
4.369LeuArg: 4.369 ± 0.694
6.515LeuSer: 6.515 ± 0.741
4.445LeuThr: 4.445 ± 0.603
5.519LeuVal: 5.519 ± 0.508
0.537LeuTrp: 0.537 ± 0.189
2.223LeuTyr: 2.223 ± 0.466
0.0LeuXaa: 0.0 ± 0.0
Met
2.836MetAla: 2.836 ± 0.474
0.383MetCys: 0.383 ± 0.158
0.92MetAsp: 0.92 ± 0.311
1.226MetGlu: 1.226 ± 0.318
1.533MetPhe: 1.533 ± 0.39
1.226MetGly: 1.226 ± 0.298
0.23MetHis: 0.23 ± 0.126
2.529MetIle: 2.529 ± 0.455
1.686MetLys: 1.686 ± 0.381
1.993MetLeu: 1.993 ± 0.518
0.843MetMet: 0.843 ± 0.31
1.84MetAsn: 1.84 ± 0.417
0.613MetPro: 0.613 ± 0.209
1.226MetGln: 1.226 ± 0.332
1.686MetArg: 1.686 ± 0.354
1.763MetSer: 1.763 ± 0.332
1.993MetThr: 1.993 ± 0.4
1.456MetVal: 1.456 ± 0.417
0.307MetTrp: 0.307 ± 0.186
0.766MetTyr: 0.766 ± 0.247
0.0MetXaa: 0.0 ± 0.0
Asn
3.296AsnAla: 3.296 ± 0.532
0.92AsnCys: 0.92 ± 0.284
2.913AsnAsp: 2.913 ± 0.334
3.219AsnGlu: 3.219 ± 0.463
2.146AsnPhe: 2.146 ± 0.346
5.902AsnGly: 5.902 ± 1.142
1.303AsnHis: 1.303 ± 0.402
2.606AsnIle: 2.606 ± 0.425
2.836AsnLys: 2.836 ± 0.375
3.526AsnLeu: 3.526 ± 0.604
1.303AsnMet: 1.303 ± 0.304
3.679AsnAsn: 3.679 ± 0.555
1.84AsnPro: 1.84 ± 0.328
2.529AsnGln: 2.529 ± 0.517
2.453AsnArg: 2.453 ± 0.469
4.369AsnSer: 4.369 ± 0.56
1.916AsnThr: 1.916 ± 0.338
4.062AsnVal: 4.062 ± 0.377
1.303AsnTrp: 1.303 ± 0.284
1.686AsnTyr: 1.686 ± 0.375
0.0AsnXaa: 0.0 ± 0.0
Pro
2.606ProAla: 2.606 ± 0.348
0.383ProCys: 0.383 ± 0.198
1.84ProAsp: 1.84 ± 0.314
2.759ProGlu: 2.759 ± 0.402
1.533ProPhe: 1.533 ± 0.309
1.763ProGly: 1.763 ± 0.35
0.46ProHis: 0.46 ± 0.184
1.993ProIle: 1.993 ± 0.371
1.15ProLys: 1.15 ± 0.335
1.763ProLeu: 1.763 ± 0.338
0.69ProMet: 0.69 ± 0.204
1.38ProAsn: 1.38 ± 0.352
0.46ProPro: 0.46 ± 0.194
1.38ProGln: 1.38 ± 0.401
1.533ProArg: 1.533 ± 0.335
1.533ProSer: 1.533 ± 0.27
1.84ProThr: 1.84 ± 0.359
2.376ProVal: 2.376 ± 0.474
0.537ProTrp: 0.537 ± 0.219
1.226ProTyr: 1.226 ± 0.301
0.0ProXaa: 0.0 ± 0.0
Gln
4.062GlnAla: 4.062 ± 1.018
0.23GlnCys: 0.23 ± 0.128
1.763GlnAsp: 1.763 ± 0.358
2.606GlnGlu: 2.606 ± 0.437
1.303GlnPhe: 1.303 ± 0.384
2.683GlnGly: 2.683 ± 0.442
0.613GlnHis: 0.613 ± 0.211
2.836GlnIle: 2.836 ± 0.667
1.916GlnLys: 1.916 ± 0.353
2.606GlnLeu: 2.606 ± 0.647
0.613GlnMet: 0.613 ± 0.2
2.146GlnAsn: 2.146 ± 0.48
0.92GlnPro: 0.92 ± 0.302
2.759GlnGln: 2.759 ± 0.793
1.38GlnArg: 1.38 ± 0.441
3.066GlnSer: 3.066 ± 0.525
1.533GlnThr: 1.533 ± 0.31
2.223GlnVal: 2.223 ± 0.373
0.537GlnTrp: 0.537 ± 0.188
1.303GlnTyr: 1.303 ± 0.388
0.0GlnXaa: 0.0 ± 0.0
Arg
4.062ArgAla: 4.062 ± 0.598
0.766ArgCys: 0.766 ± 0.357
2.069ArgAsp: 2.069 ± 0.402
3.909ArgGlu: 3.909 ± 0.591
2.606ArgPhe: 2.606 ± 0.351
2.759ArgGly: 2.759 ± 0.494
0.537ArgHis: 0.537 ± 0.17
3.756ArgIle: 3.756 ± 0.537
5.135ArgLys: 5.135 ± 0.489
3.679ArgLeu: 3.679 ± 0.576
1.073ArgMet: 1.073 ± 0.256
2.683ArgAsn: 2.683 ± 0.464
1.533ArgPro: 1.533 ± 0.378
1.686ArgGln: 1.686 ± 0.423
2.453ArgArg: 2.453 ± 0.419
2.683ArgSer: 2.683 ± 0.426
1.916ArgThr: 1.916 ± 0.416
4.062ArgVal: 4.062 ± 0.464
0.537ArgTrp: 0.537 ± 0.187
1.993ArgTyr: 1.993 ± 0.332
0.0ArgXaa: 0.0 ± 0.0
Ser
5.519SerAla: 5.519 ± 0.614
0.766SerCys: 0.766 ± 0.246
4.522SerAsp: 4.522 ± 0.576
4.905SerGlu: 4.905 ± 0.601
2.453SerPhe: 2.453 ± 0.416
6.821SerGly: 6.821 ± 0.649
0.92SerHis: 0.92 ± 0.266
4.829SerIle: 4.829 ± 0.396
4.216SerLys: 4.216 ± 0.622
5.135SerLeu: 5.135 ± 0.653
1.686SerMet: 1.686 ± 0.334
3.372SerAsn: 3.372 ± 0.575
2.146SerPro: 2.146 ± 0.407
2.299SerGln: 2.299 ± 0.385
2.989SerArg: 2.989 ± 0.526
4.982SerSer: 4.982 ± 0.829
3.602SerThr: 3.602 ± 0.519
5.059SerVal: 5.059 ± 0.598
0.69SerTrp: 0.69 ± 0.218
2.376SerTyr: 2.376 ± 0.465
0.0SerXaa: 0.0 ± 0.0
Thr
4.982ThrAla: 4.982 ± 0.646
0.537ThrCys: 0.537 ± 0.196
3.296ThrAsp: 3.296 ± 0.45
2.836ThrGlu: 2.836 ± 0.393
2.069ThrPhe: 2.069 ± 0.346
5.289ThrGly: 5.289 ± 0.62
0.537ThrHis: 0.537 ± 0.251
3.602ThrIle: 3.602 ± 0.43
3.526ThrLys: 3.526 ± 0.613
3.449ThrLeu: 3.449 ± 0.505
0.996ThrMet: 0.996 ± 0.25
3.449ThrAsn: 3.449 ± 0.526
2.146ThrPro: 2.146 ± 0.366
2.683ThrGln: 2.683 ± 0.423
2.453ThrArg: 2.453 ± 0.323
3.296ThrSer: 3.296 ± 0.352
2.529ThrThr: 2.529 ± 0.469
3.756ThrVal: 3.756 ± 0.435
0.537ThrTrp: 0.537 ± 0.173
2.223ThrTyr: 2.223 ± 0.433
0.0ThrXaa: 0.0 ± 0.0
Val
5.825ValAla: 5.825 ± 0.806
0.996ValCys: 0.996 ± 0.292
4.522ValAsp: 4.522 ± 0.491
4.445ValGlu: 4.445 ± 0.8
2.836ValPhe: 2.836 ± 0.36
3.526ValGly: 3.526 ± 0.607
1.61ValHis: 1.61 ± 0.37
3.679ValIle: 3.679 ± 0.481
6.515ValLys: 6.515 ± 0.742
4.062ValLeu: 4.062 ± 0.508
1.763ValMet: 1.763 ± 0.368
4.905ValAsn: 4.905 ± 0.521
2.146ValPro: 2.146 ± 0.485
2.223ValGln: 2.223 ± 0.61
4.292ValArg: 4.292 ± 0.602
5.672ValSer: 5.672 ± 0.597
4.292ValThr: 4.292 ± 0.737
5.365ValVal: 5.365 ± 0.792
1.073ValTrp: 1.073 ± 0.252
3.296ValTyr: 3.296 ± 0.584
0.0ValXaa: 0.0 ± 0.0
Trp
0.766TrpAla: 0.766 ± 0.201
0.077TrpCys: 0.077 ± 0.07
0.92TrpAsp: 0.92 ± 0.216
0.69TrpGlu: 0.69 ± 0.237
0.766TrpPhe: 0.766 ± 0.304
0.843TrpGly: 0.843 ± 0.224
0.307TrpHis: 0.307 ± 0.141
0.69TrpIle: 0.69 ± 0.225
1.533TrpLys: 1.533 ± 0.313
0.996TrpLeu: 0.996 ± 0.188
0.307TrpMet: 0.307 ± 0.161
0.766TrpAsn: 0.766 ± 0.181
0.23TrpPro: 0.23 ± 0.112
0.383TrpGln: 0.383 ± 0.183
0.69TrpArg: 0.69 ± 0.234
1.073TrpSer: 1.073 ± 0.42
0.23TrpThr: 0.23 ± 0.149
0.92TrpVal: 0.92 ± 0.232
0.077TrpTrp: 0.077 ± 0.065
0.23TrpTyr: 0.23 ± 0.111
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.299TyrAla: 2.299 ± 0.428
0.843TyrCys: 0.843 ± 0.206
2.529TyrAsp: 2.529 ± 0.447
2.913TyrGlu: 2.913 ± 0.583
1.456TyrPhe: 1.456 ± 0.284
2.683TyrGly: 2.683 ± 0.415
0.996TyrHis: 0.996 ± 0.249
2.453TyrIle: 2.453 ± 0.459
2.146TyrLys: 2.146 ± 0.424
2.453TyrLeu: 2.453 ± 0.409
0.766TyrMet: 0.766 ± 0.218
2.223TyrAsn: 2.223 ± 0.424
1.686TyrPro: 1.686 ± 0.329
1.456TyrGln: 1.456 ± 0.358
2.146TyrArg: 2.146 ± 0.405
2.913TyrSer: 2.913 ± 0.498
2.453TyrThr: 2.453 ± 0.379
2.759TyrVal: 2.759 ± 0.452
0.69TyrTrp: 0.69 ± 0.209
1.226TyrTyr: 1.226 ± 0.324
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 71 proteins (13048 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski