Amino acid dipepetide frequency for Mycobacterium phage Rhynn

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.651AlaAla: 12.651 ± 1.232
0.818AlaCys: 0.818 ± 0.215
6.672AlaAsp: 6.672 ± 0.644
6.042AlaGlu: 6.042 ± 0.758
3.399AlaPhe: 3.399 ± 0.485
8.056AlaGly: 8.056 ± 1.018
1.888AlaHis: 1.888 ± 0.366
4.217AlaIle: 4.217 ± 0.556
4.595AlaLys: 4.595 ± 0.647
8.56AlaLeu: 8.56 ± 0.797
2.769AlaMet: 2.769 ± 0.536
2.581AlaAsn: 2.581 ± 0.455
4.721AlaPro: 4.721 ± 0.623
2.581AlaGln: 2.581 ± 0.4
5.791AlaArg: 5.791 ± 0.606
4.658AlaSer: 4.658 ± 0.463
6.042AlaThr: 6.042 ± 0.746
8.56AlaVal: 8.56 ± 0.799
1.888AlaTrp: 1.888 ± 0.358
2.518AlaTyr: 2.518 ± 0.36
0.0AlaXaa: 0.0 ± 0.0
Cys
0.755CysAla: 0.755 ± 0.233
0.0CysCys: 0.0 ± 0.0
0.441CysAsp: 0.441 ± 0.165
0.629CysGlu: 0.629 ± 0.173
0.126CysPhe: 0.126 ± 0.089
0.818CysGly: 0.818 ± 0.303
0.189CysHis: 0.189 ± 0.105
0.315CysIle: 0.315 ± 0.153
0.189CysLys: 0.189 ± 0.1
0.441CysLeu: 0.441 ± 0.21
0.189CysMet: 0.189 ± 0.11
0.189CysAsn: 0.189 ± 0.112
0.189CysPro: 0.189 ± 0.114
0.252CysGln: 0.252 ± 0.155
0.629CysArg: 0.629 ± 0.219
0.566CysSer: 0.566 ± 0.191
0.189CysThr: 0.189 ± 0.102
0.252CysVal: 0.252 ± 0.128
0.252CysTrp: 0.252 ± 0.119
0.252CysTyr: 0.252 ± 0.133
0.0CysXaa: 0.0 ± 0.0
Asp
6.483AspAla: 6.483 ± 0.62
0.692AspCys: 0.692 ± 0.249
4.783AspAsp: 4.783 ± 0.453
3.902AspGlu: 3.902 ± 0.468
2.455AspPhe: 2.455 ± 0.374
5.979AspGly: 5.979 ± 0.614
1.133AspHis: 1.133 ± 0.261
2.581AspIle: 2.581 ± 0.418
2.832AspLys: 2.832 ± 0.412
6.483AspLeu: 6.483 ± 0.704
1.322AspMet: 1.322 ± 0.256
2.14AspAsn: 2.14 ± 0.418
5.35AspPro: 5.35 ± 0.585
1.511AspGln: 1.511 ± 0.294
3.588AspArg: 3.588 ± 0.459
3.273AspSer: 3.273 ± 0.509
3.651AspThr: 3.651 ± 0.464
5.224AspVal: 5.224 ± 0.601
1.762AspTrp: 1.762 ± 0.337
2.203AspTyr: 2.203 ± 0.33
0.0AspXaa: 0.0 ± 0.0
Glu
6.105GluAla: 6.105 ± 0.666
0.252GluCys: 0.252 ± 0.155
5.287GluAsp: 5.287 ± 0.546
4.909GluGlu: 4.909 ± 0.585
1.951GluPhe: 1.951 ± 0.372
3.902GluGly: 3.902 ± 0.466
1.385GluHis: 1.385 ± 0.366
3.588GluIle: 3.588 ± 0.528
2.455GluLys: 2.455 ± 0.423
6.798GluLeu: 6.798 ± 0.608
1.636GluMet: 1.636 ± 0.285
1.574GluAsn: 1.574 ± 0.306
2.644GluPro: 2.644 ± 0.368
2.581GluGln: 2.581 ± 0.406
4.154GluArg: 4.154 ± 0.532
3.588GluSer: 3.588 ± 0.412
4.091GluThr: 4.091 ± 0.494
5.098GluVal: 5.098 ± 0.556
1.762GluTrp: 1.762 ± 0.391
2.203GluTyr: 2.203 ± 0.383
0.0GluXaa: 0.0 ± 0.0
Phe
2.769PheAla: 2.769 ± 0.435
0.315PheCys: 0.315 ± 0.154
2.769PheAsp: 2.769 ± 0.322
2.329PheGlu: 2.329 ± 0.353
0.629PhePhe: 0.629 ± 0.179
3.651PheGly: 3.651 ± 0.547
0.629PheHis: 0.629 ± 0.244
1.322PheIle: 1.322 ± 0.244
1.322PheLys: 1.322 ± 0.272
2.203PheLeu: 2.203 ± 0.47
0.692PheMet: 0.692 ± 0.244
1.196PheAsn: 1.196 ± 0.307
1.762PhePro: 1.762 ± 0.323
0.881PheGln: 0.881 ± 0.259
1.825PheArg: 1.825 ± 0.342
1.699PheSer: 1.699 ± 0.32
2.014PheThr: 2.014 ± 0.352
1.951PheVal: 1.951 ± 0.43
0.504PheTrp: 0.504 ± 0.187
1.007PheTyr: 1.007 ± 0.225
0.0PheXaa: 0.0 ± 0.0
Gly
7.616GlyAla: 7.616 ± 1.07
0.504GlyCys: 0.504 ± 0.166
5.791GlyAsp: 5.791 ± 0.592
4.783GlyGlu: 4.783 ± 0.504
3.21GlyPhe: 3.21 ± 0.617
10.196GlyGly: 10.196 ± 2.801
1.699GlyHis: 1.699 ± 0.325
4.469GlyIle: 4.469 ± 0.755
3.588GlyLys: 3.588 ± 0.513
7.868GlyLeu: 7.868 ± 0.768
1.951GlyMet: 1.951 ± 0.353
3.588GlyAsn: 3.588 ± 0.515
3.776GlyPro: 3.776 ± 0.577
2.455GlyGln: 2.455 ± 0.311
4.721GlyArg: 4.721 ± 0.604
6.231GlySer: 6.231 ± 0.852
4.909GlyThr: 4.909 ± 0.711
5.224GlyVal: 5.224 ± 0.503
2.706GlyTrp: 2.706 ± 0.396
2.581GlyTyr: 2.581 ± 0.414
0.0GlyXaa: 0.0 ± 0.0
His
1.636HisAla: 1.636 ± 0.383
0.189HisCys: 0.189 ± 0.163
1.259HisAsp: 1.259 ± 0.206
1.699HisGlu: 1.699 ± 0.342
0.629HisPhe: 0.629 ± 0.182
1.762HisGly: 1.762 ± 0.394
0.566HisHis: 0.566 ± 0.164
0.818HisIle: 0.818 ± 0.221
0.881HisLys: 0.881 ± 0.29
1.574HisLeu: 1.574 ± 0.333
0.189HisMet: 0.189 ± 0.104
0.252HisAsn: 0.252 ± 0.12
1.448HisPro: 1.448 ± 0.293
0.818HisGln: 0.818 ± 0.206
1.636HisArg: 1.636 ± 0.339
0.504HisSer: 0.504 ± 0.167
0.944HisThr: 0.944 ± 0.229
1.636HisVal: 1.636 ± 0.303
0.566HisTrp: 0.566 ± 0.153
0.818HisTyr: 0.818 ± 0.265
0.0HisXaa: 0.0 ± 0.0
Ile
5.979IleAla: 5.979 ± 0.627
0.378IleCys: 0.378 ± 0.153
3.651IleAsp: 3.651 ± 0.457
3.713IleGlu: 3.713 ± 0.515
1.007IlePhe: 1.007 ± 0.237
3.965IleGly: 3.965 ± 0.547
0.629IleHis: 0.629 ± 0.202
1.636IleIle: 1.636 ± 0.291
1.825IleLys: 1.825 ± 0.321
3.336IleLeu: 3.336 ± 0.428
0.629IleMet: 0.629 ± 0.219
1.825IleAsn: 1.825 ± 0.34
3.021IlePro: 3.021 ± 0.389
1.511IleGln: 1.511 ± 0.33
3.399IleArg: 3.399 ± 0.513
3.084IleSer: 3.084 ± 0.422
3.462IleThr: 3.462 ± 0.43
2.832IleVal: 2.832 ± 0.563
0.692IleTrp: 0.692 ± 0.203
1.636IleTyr: 1.636 ± 0.287
0.0IleXaa: 0.0 ± 0.0
Lys
3.588LysAla: 3.588 ± 0.529
0.441LysCys: 0.441 ± 0.184
2.644LysAsp: 2.644 ± 0.43
2.329LysGlu: 2.329 ± 0.345
1.448LysPhe: 1.448 ± 0.28
2.581LysGly: 2.581 ± 0.383
1.448LysHis: 1.448 ± 0.325
2.392LysIle: 2.392 ± 0.411
1.762LysLys: 1.762 ± 0.361
3.525LysLeu: 3.525 ± 0.526
1.007LysMet: 1.007 ± 0.206
1.511LysAsn: 1.511 ± 0.278
2.832LysPro: 2.832 ± 0.455
1.699LysGln: 1.699 ± 0.385
2.958LysArg: 2.958 ± 0.493
2.706LysSer: 2.706 ± 0.344
2.581LysThr: 2.581 ± 0.326
3.336LysVal: 3.336 ± 0.566
0.755LysTrp: 0.755 ± 0.222
1.07LysTyr: 1.07 ± 0.3
0.0LysXaa: 0.0 ± 0.0
Leu
9.441LeuAla: 9.441 ± 0.865
0.378LeuCys: 0.378 ± 0.141
6.672LeuAsp: 6.672 ± 0.527
5.539LeuGlu: 5.539 ± 0.632
2.203LeuPhe: 2.203 ± 0.357
7.679LeuGly: 7.679 ± 0.799
1.574LeuHis: 1.574 ± 0.324
4.217LeuIle: 4.217 ± 0.584
3.965LeuLys: 3.965 ± 0.477
6.042LeuLeu: 6.042 ± 0.64
1.511LeuMet: 1.511 ± 0.259
2.958LeuAsn: 2.958 ± 0.432
5.539LeuPro: 5.539 ± 0.582
2.832LeuGln: 2.832 ± 0.537
6.357LeuArg: 6.357 ± 0.573
5.476LeuSer: 5.476 ± 0.542
5.602LeuThr: 5.602 ± 0.495
4.091LeuVal: 4.091 ± 0.665
1.007LeuTrp: 1.007 ± 0.303
2.455LeuTyr: 2.455 ± 0.355
0.0LeuXaa: 0.0 ± 0.0
Met
2.266MetAla: 2.266 ± 0.305
0.063MetCys: 0.063 ± 0.065
1.07MetAsp: 1.07 ± 0.221
1.322MetGlu: 1.322 ± 0.311
0.629MetPhe: 0.629 ± 0.171
1.448MetGly: 1.448 ± 0.287
0.441MetHis: 0.441 ± 0.148
0.755MetIle: 0.755 ± 0.216
1.133MetLys: 1.133 ± 0.268
0.944MetLeu: 0.944 ± 0.235
0.126MetMet: 0.126 ± 0.073
1.007MetAsn: 1.007 ± 0.229
1.196MetPro: 1.196 ± 0.251
0.566MetGln: 0.566 ± 0.171
1.196MetArg: 1.196 ± 0.27
2.455MetSer: 2.455 ± 0.458
2.266MetThr: 2.266 ± 0.291
0.944MetVal: 0.944 ± 0.281
0.315MetTrp: 0.315 ± 0.128
0.378MetTyr: 0.378 ± 0.148
0.0MetXaa: 0.0 ± 0.0
Asn
3.399AsnAla: 3.399 ± 0.453
0.063AsnCys: 0.063 ± 0.065
2.14AsnAsp: 2.14 ± 0.41
1.825AsnGlu: 1.825 ± 0.328
1.007AsnPhe: 1.007 ± 0.285
3.839AsnGly: 3.839 ± 0.544
0.755AsnHis: 0.755 ± 0.188
1.825AsnIle: 1.825 ± 0.367
0.566AsnLys: 0.566 ± 0.205
2.455AsnLeu: 2.455 ± 0.336
0.441AsnMet: 0.441 ± 0.153
0.944AsnAsn: 0.944 ± 0.232
2.581AsnPro: 2.581 ± 0.344
1.07AsnGln: 1.07 ± 0.237
1.322AsnArg: 1.322 ± 0.363
1.762AsnSer: 1.762 ± 0.4
2.077AsnThr: 2.077 ± 0.336
2.518AsnVal: 2.518 ± 0.406
0.881AsnTrp: 0.881 ± 0.181
1.196AsnTyr: 1.196 ± 0.257
0.0AsnXaa: 0.0 ± 0.0
Pro
5.413ProAla: 5.413 ± 0.587
0.504ProCys: 0.504 ± 0.199
4.154ProAsp: 4.154 ± 0.484
4.154ProGlu: 4.154 ± 0.484
2.392ProPhe: 2.392 ± 0.426
4.846ProGly: 4.846 ± 0.582
1.007ProHis: 1.007 ± 0.264
2.518ProIle: 2.518 ± 0.414
2.266ProLys: 2.266 ± 0.298
4.469ProLeu: 4.469 ± 0.559
1.007ProMet: 1.007 ± 0.238
1.448ProAsn: 1.448 ± 0.293
3.021ProPro: 3.021 ± 0.527
1.574ProGln: 1.574 ± 0.306
2.706ProArg: 2.706 ± 0.47
3.902ProSer: 3.902 ± 0.499
3.902ProThr: 3.902 ± 0.575
3.588ProVal: 3.588 ± 0.418
0.881ProTrp: 0.881 ± 0.279
1.699ProTyr: 1.699 ± 0.38
0.0ProXaa: 0.0 ± 0.0
Gln
2.958GlnAla: 2.958 ± 0.481
0.126GlnCys: 0.126 ± 0.089
1.259GlnAsp: 1.259 ± 0.316
1.762GlnGlu: 1.762 ± 0.312
1.07GlnPhe: 1.07 ± 0.257
2.329GlnGly: 2.329 ± 0.343
0.629GlnHis: 0.629 ± 0.162
2.644GlnIle: 2.644 ± 0.523
1.07GlnLys: 1.07 ± 0.285
3.776GlnLeu: 3.776 ± 0.503
0.755GlnMet: 0.755 ± 0.226
0.441GlnAsn: 0.441 ± 0.155
1.951GlnPro: 1.951 ± 0.379
1.636GlnGln: 1.636 ± 0.391
1.825GlnArg: 1.825 ± 0.352
1.762GlnSer: 1.762 ± 0.324
1.699GlnThr: 1.699 ± 0.317
2.644GlnVal: 2.644 ± 0.396
0.692GlnTrp: 0.692 ± 0.163
0.378GlnTyr: 0.378 ± 0.112
0.0GlnXaa: 0.0 ± 0.0
Arg
5.161ArgAla: 5.161 ± 0.635
0.692ArgCys: 0.692 ± 0.256
3.525ArgAsp: 3.525 ± 0.405
4.406ArgGlu: 4.406 ± 0.651
1.825ArgPhe: 1.825 ± 0.322
4.658ArgGly: 4.658 ± 0.608
0.944ArgHis: 0.944 ± 0.248
3.462ArgIle: 3.462 ± 0.544
3.776ArgLys: 3.776 ± 0.601
6.042ArgLeu: 6.042 ± 0.784
2.014ArgMet: 2.014 ± 0.358
2.266ArgAsn: 2.266 ± 0.424
2.266ArgPro: 2.266 ± 0.366
2.014ArgGln: 2.014 ± 0.334
5.413ArgArg: 5.413 ± 0.667
3.462ArgSer: 3.462 ± 0.523
2.895ArgThr: 2.895 ± 0.489
4.846ArgVal: 4.846 ± 0.565
1.259ArgTrp: 1.259 ± 0.262
1.574ArgTyr: 1.574 ± 0.294
0.0ArgXaa: 0.0 ± 0.0
Ser
6.168SerAla: 6.168 ± 0.74
0.566SerCys: 0.566 ± 0.208
3.21SerAsp: 3.21 ± 0.392
4.217SerGlu: 4.217 ± 0.499
1.825SerPhe: 1.825 ± 0.41
6.546SerGly: 6.546 ± 0.999
1.196SerHis: 1.196 ± 0.248
2.706SerIle: 2.706 ± 0.359
2.769SerLys: 2.769 ± 0.386
4.972SerLeu: 4.972 ± 0.477
1.636SerMet: 1.636 ± 0.312
2.329SerAsn: 2.329 ± 0.454
3.147SerPro: 3.147 ± 0.446
1.762SerGln: 1.762 ± 0.299
2.832SerArg: 2.832 ± 0.334
3.462SerSer: 3.462 ± 0.648
3.084SerThr: 3.084 ± 0.456
4.154SerVal: 4.154 ± 0.555
1.196SerTrp: 1.196 ± 0.301
1.448SerTyr: 1.448 ± 0.303
0.0SerXaa: 0.0 ± 0.0
Thr
5.728ThrAla: 5.728 ± 0.701
0.126ThrCys: 0.126 ± 0.09
4.091ThrAsp: 4.091 ± 0.573
3.902ThrGlu: 3.902 ± 0.477
2.329ThrPhe: 2.329 ± 0.445
6.294ThrGly: 6.294 ± 0.637
1.07ThrHis: 1.07 ± 0.32
2.392ThrIle: 2.392 ± 0.615
2.706ThrLys: 2.706 ± 0.341
5.853ThrLeu: 5.853 ± 0.603
0.692ThrMet: 0.692 ± 0.172
1.762ThrAsn: 1.762 ± 0.298
3.839ThrPro: 3.839 ± 0.495
1.574ThrGln: 1.574 ± 0.278
3.462ThrArg: 3.462 ± 0.594
4.028ThrSer: 4.028 ± 0.573
4.28ThrThr: 4.28 ± 0.58
5.916ThrVal: 5.916 ± 0.659
1.007ThrTrp: 1.007 ± 0.262
1.825ThrTyr: 1.825 ± 0.351
0.0ThrXaa: 0.0 ± 0.0
Val
6.735ValAla: 6.735 ± 0.652
0.315ValCys: 0.315 ± 0.14
5.161ValAsp: 5.161 ± 0.487
5.098ValGlu: 5.098 ± 0.577
2.014ValPhe: 2.014 ± 0.462
5.098ValGly: 5.098 ± 0.572
1.448ValHis: 1.448 ± 0.231
3.651ValIle: 3.651 ± 0.455
3.273ValLys: 3.273 ± 0.484
5.665ValLeu: 5.665 ± 0.494
0.944ValMet: 0.944 ± 0.296
2.644ValAsn: 2.644 ± 0.41
3.965ValPro: 3.965 ± 0.542
2.077ValGln: 2.077 ± 0.355
4.972ValArg: 4.972 ± 0.681
4.217ValSer: 4.217 ± 0.489
5.728ValThr: 5.728 ± 0.7
5.224ValVal: 5.224 ± 0.771
1.322ValTrp: 1.322 ± 0.284
2.266ValTyr: 2.266 ± 0.38
0.0ValXaa: 0.0 ± 0.0
Trp
1.636TrpAla: 1.636 ± 0.358
0.189TrpCys: 0.189 ± 0.117
1.511TrpAsp: 1.511 ± 0.295
1.07TrpGlu: 1.07 ± 0.204
0.881TrpPhe: 0.881 ± 0.229
1.762TrpGly: 1.762 ± 0.297
0.441TrpHis: 0.441 ± 0.169
1.133TrpIle: 1.133 ± 0.288
0.315TrpLys: 0.315 ± 0.21
1.888TrpLeu: 1.888 ± 0.346
0.441TrpMet: 0.441 ± 0.207
0.755TrpAsn: 0.755 ± 0.209
1.007TrpPro: 1.007 ± 0.248
0.881TrpGln: 0.881 ± 0.199
1.133TrpArg: 1.133 ± 0.294
1.007TrpSer: 1.007 ± 0.256
1.636TrpThr: 1.636 ± 0.394
1.888TrpVal: 1.888 ± 0.333
0.566TrpTrp: 0.566 ± 0.172
0.378TrpTyr: 0.378 ± 0.138
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.518TyrAla: 2.518 ± 0.388
0.252TyrCys: 0.252 ± 0.163
1.259TyrAsp: 1.259 ± 0.292
2.329TyrGlu: 2.329 ± 0.369
0.441TyrPhe: 0.441 ± 0.142
2.455TyrGly: 2.455 ± 0.41
0.755TyrHis: 0.755 ± 0.192
1.636TyrIle: 1.636 ± 0.385
1.322TyrLys: 1.322 ± 0.25
2.644TyrLeu: 2.644 ± 0.39
0.504TyrMet: 0.504 ± 0.156
1.259TyrAsn: 1.259 ± 0.307
1.196TyrPro: 1.196 ± 0.289
1.133TyrGln: 1.133 ± 0.249
2.581TyrArg: 2.581 ± 0.396
1.385TyrSer: 1.385 ± 0.266
1.825TyrThr: 1.825 ± 0.376
1.888TyrVal: 1.888 ± 0.336
0.504TyrTrp: 0.504 ± 0.195
0.441TyrTyr: 0.441 ± 0.151
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 86 proteins (15889 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski