Amino acid dipepetide frequency for Clostridium phage JD032

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.566AlaAla: 1.566 ± 0.499
1.077AlaCys: 1.077 ± 0.304
2.056AlaAsp: 2.056 ± 0.527
4.405AlaGlu: 4.405 ± 0.597
1.566AlaPhe: 1.566 ± 0.325
2.937AlaGly: 2.937 ± 0.583
0.587AlaHis: 0.587 ± 0.204
5.188AlaIle: 5.188 ± 0.761
4.209AlaLys: 4.209 ± 0.657
4.993AlaLeu: 4.993 ± 0.65
0.783AlaMet: 0.783 ± 0.272
3.426AlaAsn: 3.426 ± 0.453
1.468AlaPro: 1.468 ± 0.532
1.273AlaGln: 1.273 ± 0.391
1.86AlaArg: 1.86 ± 0.505
3.231AlaSer: 3.231 ± 0.786
3.916AlaThr: 3.916 ± 0.669
2.937AlaVal: 2.937 ± 0.631
0.881AlaTrp: 0.881 ± 0.242
2.447AlaTyr: 2.447 ± 0.544
0.0AlaXaa: 0.0 ± 0.0
Cys
0.685CysAla: 0.685 ± 0.201
0.0CysCys: 0.0 ± 0.0
0.587CysAsp: 0.587 ± 0.236
1.273CysGlu: 1.273 ± 0.316
0.294CysPhe: 0.294 ± 0.158
0.783CysGly: 0.783 ± 0.363
0.098CysHis: 0.098 ± 0.083
0.783CysIle: 0.783 ± 0.271
1.664CysLys: 1.664 ± 0.314
0.685CysLeu: 0.685 ± 0.271
0.294CysMet: 0.294 ± 0.184
0.783CysAsn: 0.783 ± 0.265
0.392CysPro: 0.392 ± 0.179
0.098CysGln: 0.098 ± 0.087
0.294CysArg: 0.294 ± 0.15
0.685CysSer: 0.685 ± 0.254
0.294CysThr: 0.294 ± 0.136
0.392CysVal: 0.392 ± 0.16
0.294CysTrp: 0.294 ± 0.15
0.489CysTyr: 0.489 ± 0.203
0.0CysXaa: 0.0 ± 0.0
Asp
2.839AspAla: 2.839 ± 0.46
0.979AspCys: 0.979 ± 0.269
3.231AspAsp: 3.231 ± 0.566
5.482AspGlu: 5.482 ± 0.813
3.231AspPhe: 3.231 ± 0.558
3.035AspGly: 3.035 ± 0.5
0.196AspHis: 0.196 ± 0.112
7.44AspIle: 7.44 ± 0.816
6.363AspLys: 6.363 ± 0.711
5.188AspLeu: 5.188 ± 0.566
1.958AspMet: 1.958 ± 0.343
2.741AspAsn: 2.741 ± 0.475
1.077AspPro: 1.077 ± 0.327
0.783AspGln: 0.783 ± 0.245
1.664AspArg: 1.664 ± 0.322
4.209AspSer: 4.209 ± 0.619
2.545AspThr: 2.545 ± 0.487
2.937AspVal: 2.937 ± 0.628
0.196AspTrp: 0.196 ± 0.157
1.958AspTyr: 1.958 ± 0.45
0.0AspXaa: 0.0 ± 0.0
Glu
5.384GluAla: 5.384 ± 0.885
1.273GluCys: 1.273 ± 0.333
4.014GluAsp: 4.014 ± 0.638
9.692GluGlu: 9.692 ± 1.389
3.916GluPhe: 3.916 ± 0.536
5.188GluGly: 5.188 ± 0.783
1.566GluHis: 1.566 ± 0.361
9.398GluIle: 9.398 ± 1.262
9.887GluLys: 9.887 ± 1.099
9.985GluLeu: 9.985 ± 1.188
2.839GluMet: 2.839 ± 0.485
6.755GluAsn: 6.755 ± 0.895
1.175GluPro: 1.175 ± 0.294
2.349GluGln: 2.349 ± 0.324
3.133GluArg: 3.133 ± 0.508
2.937GluSer: 2.937 ± 0.481
4.112GluThr: 4.112 ± 0.527
5.776GluVal: 5.776 ± 0.773
0.783GluTrp: 0.783 ± 0.277
3.818GluTyr: 3.818 ± 0.617
0.0GluXaa: 0.0 ± 0.0
Phe
1.371PheAla: 1.371 ± 0.402
0.392PheCys: 0.392 ± 0.175
2.741PheAsp: 2.741 ± 0.457
3.818PheGlu: 3.818 ± 0.651
1.077PhePhe: 1.077 ± 0.302
3.231PheGly: 3.231 ± 0.454
0.294PheHis: 0.294 ± 0.169
4.307PheIle: 4.307 ± 0.565
4.601PheLys: 4.601 ± 0.494
3.133PheLeu: 3.133 ± 0.524
0.979PheMet: 0.979 ± 0.328
3.231PheAsn: 3.231 ± 0.501
0.783PhePro: 0.783 ± 0.352
0.783PheGln: 0.783 ± 0.251
0.979PheArg: 0.979 ± 0.317
2.056PheSer: 2.056 ± 0.457
2.252PheThr: 2.252 ± 0.399
1.86PheVal: 1.86 ± 0.44
0.098PheTrp: 0.098 ± 0.089
1.371PheTyr: 1.371 ± 0.344
0.0PheXaa: 0.0 ± 0.0
Gly
3.426GlyAla: 3.426 ± 0.741
0.783GlyCys: 0.783 ± 0.265
3.133GlyAsp: 3.133 ± 0.681
5.286GlyGlu: 5.286 ± 0.631
2.741GlyPhe: 2.741 ± 0.518
5.286GlyGly: 5.286 ± 2.077
0.392GlyHis: 0.392 ± 0.2
4.405GlyIle: 4.405 ± 0.732
6.951GlyLys: 6.951 ± 0.755
3.818GlyLeu: 3.818 ± 0.634
1.664GlyMet: 1.664 ± 0.575
3.426GlyAsn: 3.426 ± 0.434
0.392GlyPro: 0.392 ± 0.217
0.685GlyGln: 0.685 ± 0.225
1.468GlyArg: 1.468 ± 0.499
3.622GlySer: 3.622 ± 0.886
3.231GlyThr: 3.231 ± 0.637
4.307GlyVal: 4.307 ± 0.538
0.783GlyTrp: 0.783 ± 0.26
2.643GlyTyr: 2.643 ± 0.585
0.0GlyXaa: 0.0 ± 0.0
His
0.294HisAla: 0.294 ± 0.145
0.0HisCys: 0.0 ± 0.0
0.294HisAsp: 0.294 ± 0.155
0.881HisGlu: 0.881 ± 0.345
0.587HisPhe: 0.587 ± 0.234
0.489HisGly: 0.489 ± 0.21
0.098HisHis: 0.098 ± 0.083
0.783HisIle: 0.783 ± 0.213
1.273HisLys: 1.273 ± 0.348
0.783HisLeu: 0.783 ± 0.256
0.294HisMet: 0.294 ± 0.196
0.783HisAsn: 0.783 ± 0.307
0.0HisPro: 0.0 ± 0.0
0.294HisGln: 0.294 ± 0.127
0.392HisArg: 0.392 ± 0.185
0.489HisSer: 0.489 ± 0.229
0.587HisThr: 0.587 ± 0.211
0.685HisVal: 0.685 ± 0.258
0.0HisTrp: 0.0 ± 0.0
0.881HisTyr: 0.881 ± 0.341
0.0HisXaa: 0.0 ± 0.0
Ile
4.503IleAla: 4.503 ± 0.617
0.881IleCys: 0.881 ± 0.365
5.776IleAsp: 5.776 ± 0.79
10.377IleGlu: 10.377 ± 1.256
2.839IlePhe: 2.839 ± 0.535
4.993IleGly: 4.993 ± 0.909
0.979IleHis: 0.979 ± 0.303
6.559IleIle: 6.559 ± 0.784
10.768IleLys: 10.768 ± 1.161
8.027IleLeu: 8.027 ± 1.186
1.958IleMet: 1.958 ± 0.414
5.972IleAsn: 5.972 ± 0.876
3.622IlePro: 3.622 ± 0.541
2.643IleGln: 2.643 ± 0.505
4.112IleArg: 4.112 ± 0.688
5.678IleSer: 5.678 ± 0.808
4.405IleThr: 4.405 ± 0.499
3.916IleVal: 3.916 ± 0.649
0.392IleTrp: 0.392 ± 0.243
4.112IleTyr: 4.112 ± 0.751
0.0IleXaa: 0.0 ± 0.0
Lys
6.265LysAla: 6.265 ± 0.672
0.881LysCys: 0.881 ± 0.299
6.657LysAsp: 6.657 ± 0.699
12.237LysGlu: 12.237 ± 1.287
3.818LysPhe: 3.818 ± 0.609
4.699LysGly: 4.699 ± 0.66
1.86LysHis: 1.86 ± 0.414
10.573LysIle: 10.573 ± 0.945
10.573LysLys: 10.573 ± 1.072
9.692LysLeu: 9.692 ± 1.178
2.839LysMet: 2.839 ± 0.519
6.07LysAsn: 6.07 ± 0.941
2.056LysPro: 2.056 ± 0.392
3.231LysGln: 3.231 ± 0.633
3.231LysArg: 3.231 ± 0.642
5.972LysSer: 5.972 ± 0.978
5.972LysThr: 5.972 ± 0.743
5.972LysVal: 5.972 ± 0.786
1.077LysTrp: 1.077 ± 0.26
4.112LysTyr: 4.112 ± 0.565
0.0LysXaa: 0.0 ± 0.0
Leu
4.601LeuAla: 4.601 ± 0.768
0.783LeuCys: 0.783 ± 0.287
6.755LeuAsp: 6.755 ± 0.784
7.146LeuGlu: 7.146 ± 1.28
3.035LeuPhe: 3.035 ± 0.529
3.426LeuGly: 3.426 ± 0.668
0.685LeuHis: 0.685 ± 0.194
5.482LeuIle: 5.482 ± 0.72
10.083LeuLys: 10.083 ± 1.18
5.874LeuLeu: 5.874 ± 0.711
2.056LeuMet: 2.056 ± 0.449
6.167LeuAsn: 6.167 ± 0.798
1.371LeuPro: 1.371 ± 0.345
2.349LeuGln: 2.349 ± 0.456
3.622LeuArg: 3.622 ± 0.654
6.07LeuSer: 6.07 ± 0.83
4.601LeuThr: 4.601 ± 0.713
3.72LeuVal: 3.72 ± 0.632
0.979LeuTrp: 0.979 ± 0.3
3.426LeuTyr: 3.426 ± 0.596
0.0LeuXaa: 0.0 ± 0.0
Met
1.958MetAla: 1.958 ± 0.606
0.098MetCys: 0.098 ± 0.09
1.175MetAsp: 1.175 ± 0.326
1.958MetGlu: 1.958 ± 0.382
1.273MetPhe: 1.273 ± 0.403
1.273MetGly: 1.273 ± 0.486
0.294MetHis: 0.294 ± 0.143
2.349MetIle: 2.349 ± 0.462
2.741MetLys: 2.741 ± 0.557
2.349MetLeu: 2.349 ± 0.561
0.196MetMet: 0.196 ± 0.119
2.154MetAsn: 2.154 ± 0.521
0.489MetPro: 0.489 ± 0.248
0.881MetGln: 0.881 ± 0.245
0.783MetArg: 0.783 ± 0.43
1.273MetSer: 1.273 ± 0.346
1.175MetThr: 1.175 ± 0.305
1.175MetVal: 1.175 ± 0.358
0.098MetTrp: 0.098 ± 0.097
0.587MetTyr: 0.587 ± 0.226
0.0MetXaa: 0.0 ± 0.0
Asn
2.741AsnAla: 2.741 ± 0.498
0.587AsnCys: 0.587 ± 0.229
3.231AsnAsp: 3.231 ± 0.639
5.286AsnGlu: 5.286 ± 0.648
2.349AsnPhe: 2.349 ± 0.403
4.405AsnGly: 4.405 ± 0.606
0.098AsnHis: 0.098 ± 0.093
7.734AsnIle: 7.734 ± 0.932
8.223AsnLys: 8.223 ± 0.944
4.405AsnLeu: 4.405 ± 0.737
1.86AsnMet: 1.86 ± 0.397
4.699AsnAsn: 4.699 ± 0.676
2.349AsnPro: 2.349 ± 0.531
1.077AsnGln: 1.077 ± 0.356
2.349AsnArg: 2.349 ± 0.407
4.601AsnSer: 4.601 ± 0.57
4.209AsnThr: 4.209 ± 0.71
3.72AsnVal: 3.72 ± 0.55
0.783AsnTrp: 0.783 ± 0.203
2.545AsnTyr: 2.545 ± 0.499
0.0AsnXaa: 0.0 ± 0.0
Pro
1.077ProAla: 1.077 ± 0.304
0.098ProCys: 0.098 ± 0.101
0.979ProAsp: 0.979 ± 0.319
1.175ProGlu: 1.175 ± 0.271
0.881ProPhe: 0.881 ± 0.245
1.175ProGly: 1.175 ± 0.269
0.489ProHis: 0.489 ± 0.185
2.349ProIle: 2.349 ± 0.512
1.566ProLys: 1.566 ± 0.419
1.468ProLeu: 1.468 ± 0.4
0.294ProMet: 0.294 ± 0.149
1.468ProAsn: 1.468 ± 0.378
0.098ProPro: 0.098 ± 0.102
0.392ProGln: 0.392 ± 0.175
1.077ProArg: 1.077 ± 0.242
2.447ProSer: 2.447 ± 0.604
1.762ProThr: 1.762 ± 0.374
1.664ProVal: 1.664 ± 0.62
0.294ProTrp: 0.294 ± 0.173
0.881ProTyr: 0.881 ± 0.263
0.0ProXaa: 0.0 ± 0.0
Gln
2.056GlnAla: 2.056 ± 0.488
0.0GlnCys: 0.0 ± 0.0
1.762GlnAsp: 1.762 ± 0.342
2.252GlnGlu: 2.252 ± 0.446
0.881GlnPhe: 0.881 ± 0.29
1.566GlnGly: 1.566 ± 0.398
0.098GlnHis: 0.098 ± 0.101
2.056GlnIle: 2.056 ± 0.449
2.154GlnLys: 2.154 ± 0.4
2.056GlnLeu: 2.056 ± 0.456
0.881GlnMet: 0.881 ± 0.37
1.175GlnAsn: 1.175 ± 0.272
0.392GlnPro: 0.392 ± 0.209
0.979GlnGln: 0.979 ± 0.437
0.979GlnArg: 0.979 ± 0.294
1.273GlnSer: 1.273 ± 0.324
1.371GlnThr: 1.371 ± 0.318
1.077GlnVal: 1.077 ± 0.279
0.098GlnTrp: 0.098 ± 0.092
1.468GlnTyr: 1.468 ± 0.286
0.0GlnXaa: 0.0 ± 0.0
Arg
1.371ArgAla: 1.371 ± 0.396
0.294ArgCys: 0.294 ± 0.152
1.077ArgAsp: 1.077 ± 0.31
4.405ArgGlu: 4.405 ± 0.673
1.273ArgPhe: 1.273 ± 0.321
2.349ArgGly: 2.349 ± 0.384
0.685ArgHis: 0.685 ± 0.288
3.72ArgIle: 3.72 ± 0.723
3.818ArgLys: 3.818 ± 0.682
1.762ArgLeu: 1.762 ± 0.413
0.685ArgMet: 0.685 ± 0.277
2.447ArgAsn: 2.447 ± 0.522
0.685ArgPro: 0.685 ± 0.257
1.077ArgGln: 1.077 ± 0.264
2.252ArgArg: 2.252 ± 0.548
1.958ArgSer: 1.958 ± 0.464
2.447ArgThr: 2.447 ± 0.455
1.273ArgVal: 1.273 ± 0.326
0.392ArgTrp: 0.392 ± 0.159
1.371ArgTyr: 1.371 ± 0.337
0.0ArgXaa: 0.0 ± 0.0
Ser
2.447SerAla: 2.447 ± 0.576
0.685SerCys: 0.685 ± 0.218
3.035SerAsp: 3.035 ± 0.562
4.601SerGlu: 4.601 ± 0.839
3.72SerPhe: 3.72 ± 0.673
4.112SerGly: 4.112 ± 0.818
0.294SerHis: 0.294 ± 0.157
4.601SerIle: 4.601 ± 0.722
6.461SerLys: 6.461 ± 0.796
5.091SerLeu: 5.091 ± 0.604
1.371SerMet: 1.371 ± 0.464
5.091SerAsn: 5.091 ± 0.879
1.468SerPro: 1.468 ± 0.391
1.468SerGln: 1.468 ± 0.323
1.762SerArg: 1.762 ± 0.337
4.209SerSer: 4.209 ± 0.965
2.741SerThr: 2.741 ± 0.623
2.839SerVal: 2.839 ± 0.512
0.881SerTrp: 0.881 ± 0.25
3.231SerTyr: 3.231 ± 0.484
0.0SerXaa: 0.0 ± 0.0
Thr
3.426ThrAla: 3.426 ± 0.651
0.587ThrCys: 0.587 ± 0.314
3.426ThrAsp: 3.426 ± 0.395
6.07ThrGlu: 6.07 ± 0.862
1.468ThrPhe: 1.468 ± 0.331
3.524ThrGly: 3.524 ± 0.815
0.196ThrHis: 0.196 ± 0.143
4.993ThrIle: 4.993 ± 0.602
6.167ThrLys: 6.167 ± 0.582
5.482ThrLeu: 5.482 ± 0.812
0.783ThrMet: 0.783 ± 0.285
3.426ThrAsn: 3.426 ± 0.471
1.468ThrPro: 1.468 ± 0.415
1.664ThrGln: 1.664 ± 0.37
1.664ThrArg: 1.664 ± 0.318
3.133ThrSer: 3.133 ± 0.629
4.112ThrThr: 4.112 ± 0.837
2.839ThrVal: 2.839 ± 0.336
0.783ThrTrp: 0.783 ± 0.229
1.86ThrTyr: 1.86 ± 0.34
0.0ThrXaa: 0.0 ± 0.0
Val
2.643ValAla: 2.643 ± 0.574
0.587ValCys: 0.587 ± 0.21
3.231ValAsp: 3.231 ± 0.478
3.622ValGlu: 3.622 ± 0.705
2.154ValPhe: 2.154 ± 0.384
3.426ValGly: 3.426 ± 0.768
0.392ValHis: 0.392 ± 0.179
4.209ValIle: 4.209 ± 0.696
5.384ValLys: 5.384 ± 0.742
4.699ValLeu: 4.699 ± 0.717
1.371ValMet: 1.371 ± 0.314
3.328ValAsn: 3.328 ± 0.648
1.175ValPro: 1.175 ± 0.245
1.86ValGln: 1.86 ± 0.438
1.371ValArg: 1.371 ± 0.629
3.328ValSer: 3.328 ± 0.563
3.622ValThr: 3.622 ± 0.674
4.112ValVal: 4.112 ± 0.552
0.392ValTrp: 0.392 ± 0.178
3.133ValTyr: 3.133 ± 0.425
0.0ValXaa: 0.0 ± 0.0
Trp
0.685TrpAla: 0.685 ± 0.293
0.392TrpCys: 0.392 ± 0.175
1.371TrpAsp: 1.371 ± 0.305
0.294TrpGlu: 0.294 ± 0.133
0.489TrpPhe: 0.489 ± 0.177
0.979TrpGly: 0.979 ± 0.272
0.196TrpHis: 0.196 ± 0.137
1.077TrpIle: 1.077 ± 0.297
0.685TrpLys: 0.685 ± 0.227
0.685TrpLeu: 0.685 ± 0.245
0.196TrpMet: 0.196 ± 0.146
0.489TrpAsn: 0.489 ± 0.191
0.0TrpPro: 0.0 ± 0.0
0.0TrpGln: 0.0 ± 0.0
0.294TrpArg: 0.294 ± 0.217
0.294TrpSer: 0.294 ± 0.163
0.294TrpThr: 0.294 ± 0.207
0.783TrpVal: 0.783 ± 0.266
0.098TrpTrp: 0.098 ± 0.092
0.392TrpTyr: 0.392 ± 0.17
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.468TyrAla: 1.468 ± 0.381
0.489TyrCys: 0.489 ± 0.259
3.524TyrAsp: 3.524 ± 0.583
3.72TyrGlu: 3.72 ± 0.54
1.762TyrPhe: 1.762 ± 0.544
1.566TyrGly: 1.566 ± 0.42
0.392TyrHis: 0.392 ± 0.197
4.503TyrIle: 4.503 ± 0.56
4.209TyrLys: 4.209 ± 0.654
2.154TyrLeu: 2.154 ± 0.47
0.881TyrMet: 0.881 ± 0.293
3.622TyrAsn: 3.622 ± 0.599
1.175TyrPro: 1.175 ± 0.327
0.685TyrGln: 0.685 ± 0.221
2.056TyrArg: 2.056 ± 0.5
2.643TyrSer: 2.643 ± 0.564
3.426TyrThr: 3.426 ± 0.557
2.056TyrVal: 2.056 ± 0.315
0.392TyrTrp: 0.392 ± 0.17
1.371TyrTyr: 1.371 ± 0.337
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 54 proteins (10216 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski