Amino acid dipepetide frequency for Gordonia phage Pleakley

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.227AlaAla: 11.227 ± 1.054
0.692AlaCys: 0.692 ± 0.208
8.26AlaAsp: 8.26 ± 0.596
6.726AlaGlu: 6.726 ± 0.618
3.215AlaPhe: 3.215 ± 0.371
8.903AlaGly: 8.903 ± 0.761
2.325AlaHis: 2.325 ± 0.35
4.748AlaIle: 4.748 ± 0.621
4.896AlaLys: 4.896 ± 0.623
8.754AlaLeu: 8.754 ± 0.993
1.83AlaMet: 1.83 ± 0.298
2.77AlaAsn: 2.77 ± 0.344
3.264AlaPro: 3.264 ± 0.459
3.709AlaGln: 3.709 ± 0.387
6.133AlaArg: 6.133 ± 0.688
4.649AlaSer: 4.649 ± 0.511
5.589AlaThr: 5.589 ± 0.651
8.21AlaVal: 8.21 ± 0.64
1.533AlaTrp: 1.533 ± 0.279
2.325AlaTyr: 2.325 ± 0.294
0.0AlaXaa: 0.0 ± 0.0
Cys
0.544CysAla: 0.544 ± 0.161
0.099CysCys: 0.099 ± 0.084
0.94CysAsp: 0.94 ± 0.239
0.346CysGlu: 0.346 ± 0.118
0.049CysPhe: 0.049 ± 0.051
0.643CysGly: 0.643 ± 0.191
0.148CysHis: 0.148 ± 0.076
0.297CysIle: 0.297 ± 0.108
0.445CysLys: 0.445 ± 0.139
0.445CysLeu: 0.445 ± 0.189
0.099CysMet: 0.099 ± 0.07
0.198CysAsn: 0.198 ± 0.084
0.594CysPro: 0.594 ± 0.215
0.247CysGln: 0.247 ± 0.102
0.445CysArg: 0.445 ± 0.13
0.544CysSer: 0.544 ± 0.201
0.445CysThr: 0.445 ± 0.144
0.742CysVal: 0.742 ± 0.178
0.247CysTrp: 0.247 ± 0.114
0.297CysTyr: 0.297 ± 0.151
0.0CysXaa: 0.0 ± 0.0
Asp
6.677AspAla: 6.677 ± 0.446
0.594AspCys: 0.594 ± 0.189
6.281AspAsp: 6.281 ± 0.674
6.232AspGlu: 6.232 ± 0.873
2.226AspPhe: 2.226 ± 0.309
6.875AspGly: 6.875 ± 0.755
1.385AspHis: 1.385 ± 0.243
3.413AspIle: 3.413 ± 0.46
2.325AspLys: 2.325 ± 0.348
6.43AspLeu: 6.43 ± 0.591
1.583AspMet: 1.583 ± 0.261
2.275AspAsn: 2.275 ± 0.334
5.292AspPro: 5.292 ± 0.6
2.325AspGln: 2.325 ± 0.365
5.094AspArg: 5.094 ± 0.721
4.105AspSer: 4.105 ± 0.403
2.671AspThr: 2.671 ± 0.32
4.402AspVal: 4.402 ± 0.542
1.385AspTrp: 1.385 ± 0.303
2.325AspTyr: 2.325 ± 0.372
0.0AspXaa: 0.0 ± 0.0
Glu
6.726GluAla: 6.726 ± 0.567
0.247GluCys: 0.247 ± 0.123
4.352GluAsp: 4.352 ± 0.604
4.699GluGlu: 4.699 ± 0.623
2.671GluPhe: 2.671 ± 0.384
4.797GluGly: 4.797 ± 0.45
1.484GluHis: 1.484 ± 0.27
3.957GluIle: 3.957 ± 0.458
2.968GluLys: 2.968 ± 0.442
4.946GluLeu: 4.946 ± 0.624
2.077GluMet: 2.077 ± 0.343
2.72GluAsn: 2.72 ± 0.351
2.374GluPro: 2.374 ± 0.457
2.572GluGln: 2.572 ± 0.359
4.896GluArg: 4.896 ± 0.679
3.413GluSer: 3.413 ± 0.325
3.462GluThr: 3.462 ± 0.375
4.352GluVal: 4.352 ± 0.548
1.138GluTrp: 1.138 ± 0.24
2.226GluTyr: 2.226 ± 0.375
0.0GluXaa: 0.0 ± 0.0
Phe
2.918PheAla: 2.918 ± 0.335
0.346PheCys: 0.346 ± 0.11
3.066PheAsp: 3.066 ± 0.469
2.621PheGlu: 2.621 ± 0.284
0.94PhePhe: 0.94 ± 0.219
2.968PheGly: 2.968 ± 0.403
0.594PheHis: 0.594 ± 0.152
1.434PheIle: 1.434 ± 0.248
0.791PheLys: 0.791 ± 0.177
2.572PheLeu: 2.572 ± 0.347
0.544PheMet: 0.544 ± 0.159
0.791PheAsn: 0.791 ± 0.197
1.335PhePro: 1.335 ± 0.208
0.841PheGln: 0.841 ± 0.215
2.423PheArg: 2.423 ± 0.343
1.879PheSer: 1.879 ± 0.317
1.583PheThr: 1.583 ± 0.252
2.077PheVal: 2.077 ± 0.296
0.841PheTrp: 0.841 ± 0.209
1.088PheTyr: 1.088 ± 0.211
0.0PheXaa: 0.0 ± 0.0
Gly
7.468GlyAla: 7.468 ± 0.87
0.841GlyCys: 0.841 ± 0.212
6.529GlyAsp: 6.529 ± 0.684
5.045GlyGlu: 5.045 ± 0.562
3.017GlyPhe: 3.017 ± 0.347
9.1GlyGly: 9.1 ± 1.585
1.286GlyHis: 1.286 ± 0.249
4.155GlyIle: 4.155 ± 0.436
5.094GlyLys: 5.094 ± 0.544
6.677GlyLeu: 6.677 ± 0.775
2.423GlyMet: 2.423 ± 0.42
2.621GlyAsn: 2.621 ± 0.317
4.155GlyPro: 4.155 ± 0.625
3.066GlyGln: 3.066 ± 0.39
5.144GlyArg: 5.144 ± 0.539
5.342GlySer: 5.342 ± 0.622
6.083GlyThr: 6.083 ± 0.763
6.133GlyVal: 6.133 ± 0.659
1.583GlyTrp: 1.583 ± 0.25
2.918GlyTyr: 2.918 ± 0.374
0.0GlyXaa: 0.0 ± 0.0
His
1.484HisAla: 1.484 ± 0.293
0.049HisCys: 0.049 ± 0.045
0.742HisAsp: 0.742 ± 0.214
0.89HisGlu: 0.89 ± 0.204
0.89HisPhe: 0.89 ± 0.221
1.533HisGly: 1.533 ± 0.274
0.445HisHis: 0.445 ± 0.154
0.89HisIle: 0.89 ± 0.233
0.445HisLys: 0.445 ± 0.149
1.385HisLeu: 1.385 ± 0.279
0.396HisMet: 0.396 ± 0.138
0.594HisAsn: 0.594 ± 0.166
1.484HisPro: 1.484 ± 0.322
0.643HisGln: 0.643 ± 0.177
1.879HisArg: 1.879 ± 0.351
0.841HisSer: 0.841 ± 0.22
1.138HisThr: 1.138 ± 0.248
1.533HisVal: 1.533 ± 0.232
0.445HisTrp: 0.445 ± 0.141
0.643HisTyr: 0.643 ± 0.173
0.0HisXaa: 0.0 ± 0.0
Ile
4.797IleAla: 4.797 ± 0.471
0.396IleCys: 0.396 ± 0.149
3.066IleAsp: 3.066 ± 0.372
4.946IleGlu: 4.946 ± 0.519
1.138IlePhe: 1.138 ± 0.275
4.649IleGly: 4.649 ± 0.614
1.088IleHis: 1.088 ± 0.23
2.473IleIle: 2.473 ± 0.336
1.879IleLys: 1.879 ± 0.342
3.413IleLeu: 3.413 ± 0.408
0.841IleMet: 0.841 ± 0.172
2.127IleAsn: 2.127 ± 0.293
2.671IlePro: 2.671 ± 0.299
1.236IleGln: 1.236 ± 0.256
2.621IleArg: 2.621 ± 0.368
2.176IleSer: 2.176 ± 0.287
3.314IleThr: 3.314 ± 0.43
3.066IleVal: 3.066 ± 0.359
0.89IleTrp: 0.89 ± 0.218
1.088IleTyr: 1.088 ± 0.222
0.0IleXaa: 0.0 ± 0.0
Lys
4.501LysAla: 4.501 ± 0.416
0.297LysCys: 0.297 ± 0.105
3.314LysAsp: 3.314 ± 0.399
1.484LysGlu: 1.484 ± 0.258
1.039LysPhe: 1.039 ± 0.236
3.957LysGly: 3.957 ± 0.355
0.791LysHis: 0.791 ± 0.223
2.473LysIle: 2.473 ± 0.291
2.72LysLys: 2.72 ± 0.42
3.759LysLeu: 3.759 ± 0.467
1.236LysMet: 1.236 ± 0.277
1.731LysAsn: 1.731 ± 0.292
2.176LysPro: 2.176 ± 0.415
1.879LysGln: 1.879 ± 0.27
2.671LysArg: 2.671 ± 0.402
2.671LysSer: 2.671 ± 0.331
3.017LysThr: 3.017 ± 0.353
3.61LysVal: 3.61 ± 0.478
0.692LysTrp: 0.692 ± 0.149
1.187LysTyr: 1.187 ± 0.21
0.0LysXaa: 0.0 ± 0.0
Leu
9.249LeuAla: 9.249 ± 0.779
1.088LeuCys: 1.088 ± 0.274
5.836LeuAsp: 5.836 ± 0.682
3.264LeuGlu: 3.264 ± 0.36
2.176LeuPhe: 2.176 ± 0.366
6.529LeuGly: 6.529 ± 0.852
1.484LeuHis: 1.484 ± 0.321
4.105LeuIle: 4.105 ± 0.554
3.561LeuLys: 3.561 ± 0.403
5.094LeuLeu: 5.094 ± 0.424
1.879LeuMet: 1.879 ± 0.266
2.325LeuAsn: 2.325 ± 0.432
4.303LeuPro: 4.303 ± 0.554
2.077LeuGln: 2.077 ± 0.289
6.133LeuArg: 6.133 ± 0.481
4.797LeuSer: 4.797 ± 0.466
5.44LeuThr: 5.44 ± 0.562
5.045LeuVal: 5.045 ± 0.503
1.335LeuTrp: 1.335 ± 0.268
1.484LeuTyr: 1.484 ± 0.281
0.0LeuXaa: 0.0 ± 0.0
Met
2.671MetAla: 2.671 ± 0.435
0.049MetCys: 0.049 ± 0.044
1.039MetAsp: 1.039 ± 0.19
1.286MetGlu: 1.286 ± 0.214
0.989MetPhe: 0.989 ± 0.176
1.632MetGly: 1.632 ± 0.305
0.198MetHis: 0.198 ± 0.123
1.385MetIle: 1.385 ± 0.231
1.138MetLys: 1.138 ± 0.254
1.484MetLeu: 1.484 ± 0.28
0.346MetMet: 0.346 ± 0.147
1.088MetAsn: 1.088 ± 0.215
1.138MetPro: 1.138 ± 0.214
0.742MetGln: 0.742 ± 0.246
1.187MetArg: 1.187 ± 0.282
2.028MetSer: 2.028 ± 0.37
2.226MetThr: 2.226 ± 0.376
1.138MetVal: 1.138 ± 0.226
0.445MetTrp: 0.445 ± 0.13
0.297MetTyr: 0.297 ± 0.103
0.0MetXaa: 0.0 ± 0.0
Asn
2.918AsnAla: 2.918 ± 0.489
0.247AsnCys: 0.247 ± 0.096
3.066AsnAsp: 3.066 ± 0.351
2.127AsnGlu: 2.127 ± 0.273
1.236AsnPhe: 1.236 ± 0.215
3.858AsnGly: 3.858 ± 0.468
0.544AsnHis: 0.544 ± 0.168
1.138AsnIle: 1.138 ± 0.232
1.533AsnLys: 1.533 ± 0.336
2.127AsnLeu: 2.127 ± 0.314
0.495AsnMet: 0.495 ± 0.148
1.385AsnAsn: 1.385 ± 0.257
2.226AsnPro: 2.226 ± 0.286
0.742AsnGln: 0.742 ± 0.169
2.72AsnArg: 2.72 ± 0.299
1.286AsnSer: 1.286 ± 0.272
1.731AsnThr: 1.731 ± 0.275
2.374AsnVal: 2.374 ± 0.374
0.94AsnTrp: 0.94 ± 0.192
1.187AsnTyr: 1.187 ± 0.237
0.0AsnXaa: 0.0 ± 0.0
Pro
4.797ProAla: 4.797 ± 0.631
0.297ProCys: 0.297 ± 0.129
4.056ProAsp: 4.056 ± 0.51
4.699ProGlu: 4.699 ± 0.677
1.385ProPhe: 1.385 ± 0.26
4.402ProGly: 4.402 ± 0.409
0.742ProHis: 0.742 ± 0.203
2.423ProIle: 2.423 ± 0.388
2.72ProLys: 2.72 ± 0.422
3.66ProLeu: 3.66 ± 0.347
1.632ProMet: 1.632 ± 0.319
1.583ProAsn: 1.583 ± 0.28
2.325ProPro: 2.325 ± 0.377
1.434ProGln: 1.434 ± 0.247
3.759ProArg: 3.759 ± 0.521
3.017ProSer: 3.017 ± 0.428
3.314ProThr: 3.314 ± 0.413
3.561ProVal: 3.561 ± 0.489
0.544ProTrp: 0.544 ± 0.162
0.989ProTyr: 0.989 ± 0.16
0.0ProXaa: 0.0 ± 0.0
Gln
3.561GlnAla: 3.561 ± 0.419
0.346GlnCys: 0.346 ± 0.139
1.385GlnAsp: 1.385 ± 0.237
2.077GlnGlu: 2.077 ± 0.337
1.236GlnPhe: 1.236 ± 0.256
2.325GlnGly: 2.325 ± 0.347
0.148GlnHis: 0.148 ± 0.076
1.929GlnIle: 1.929 ± 0.297
1.286GlnLys: 1.286 ± 0.2
2.127GlnLeu: 2.127 ± 0.374
0.94GlnMet: 0.94 ± 0.24
1.088GlnAsn: 1.088 ± 0.221
1.385GlnPro: 1.385 ± 0.274
1.434GlnGln: 1.434 ± 0.283
2.671GlnArg: 2.671 ± 0.395
1.484GlnSer: 1.484 ± 0.272
2.028GlnThr: 2.028 ± 0.274
2.869GlnVal: 2.869 ± 0.351
1.039GlnTrp: 1.039 ± 0.216
0.742GlnTyr: 0.742 ± 0.206
0.0GlnXaa: 0.0 ± 0.0
Arg
7.023ArgAla: 7.023 ± 0.663
0.495ArgCys: 0.495 ± 0.149
5.045ArgAsp: 5.045 ± 0.636
5.045ArgGlu: 5.045 ± 0.646
2.423ArgPhe: 2.423 ± 0.475
5.49ArgGly: 5.49 ± 0.454
1.286ArgHis: 1.286 ± 0.282
2.325ArgIle: 2.325 ± 0.36
3.165ArgLys: 3.165 ± 0.388
5.688ArgLeu: 5.688 ± 0.501
1.929ArgMet: 1.929 ± 0.326
2.275ArgAsn: 2.275 ± 0.267
2.869ArgPro: 2.869 ± 0.324
2.473ArgGln: 2.473 ± 0.351
6.133ArgArg: 6.133 ± 0.622
3.61ArgSer: 3.61 ± 0.411
4.056ArgThr: 4.056 ± 0.44
4.55ArgVal: 4.55 ± 0.591
1.484ArgTrp: 1.484 ± 0.271
2.127ArgTyr: 2.127 ± 0.328
0.0ArgXaa: 0.0 ± 0.0
Ser
6.43SerAla: 6.43 ± 0.732
0.198SerCys: 0.198 ± 0.081
4.006SerAsp: 4.006 ± 0.529
3.561SerGlu: 3.561 ± 0.416
1.484SerPhe: 1.484 ± 0.297
5.144SerGly: 5.144 ± 0.545
0.89SerHis: 0.89 ± 0.267
2.621SerIle: 2.621 ± 0.328
2.968SerLys: 2.968 ± 0.345
3.413SerLeu: 3.413 ± 0.412
1.335SerMet: 1.335 ± 0.244
2.028SerAsn: 2.028 ± 0.373
2.819SerPro: 2.819 ± 0.411
1.484SerGln: 1.484 ± 0.31
3.413SerArg: 3.413 ± 0.389
2.374SerSer: 2.374 ± 0.375
3.61SerThr: 3.61 ± 0.478
3.858SerVal: 3.858 ± 0.434
0.989SerTrp: 0.989 ± 0.22
1.138SerTyr: 1.138 ± 0.231
0.0SerXaa: 0.0 ± 0.0
Thr
6.43ThrAla: 6.43 ± 0.637
0.495ThrCys: 0.495 ± 0.175
3.957ThrAsp: 3.957 ± 0.418
3.512ThrGlu: 3.512 ± 0.398
2.226ThrPhe: 2.226 ± 0.331
6.133ThrGly: 6.133 ± 0.618
1.039ThrHis: 1.039 ± 0.22
2.819ThrIle: 2.819 ± 0.381
2.72ThrLys: 2.72 ± 0.374
5.144ThrLeu: 5.144 ± 0.486
0.989ThrMet: 0.989 ± 0.205
2.127ThrAsn: 2.127 ± 0.281
3.561ThrPro: 3.561 ± 0.344
1.879ThrGln: 1.879 ± 0.304
3.561ThrArg: 3.561 ± 0.389
2.968ThrSer: 2.968 ± 0.388
4.797ThrThr: 4.797 ± 0.527
5.094ThrVal: 5.094 ± 0.475
1.286ThrTrp: 1.286 ± 0.26
1.781ThrTyr: 1.781 ± 0.313
0.0ThrXaa: 0.0 ± 0.0
Val
6.133ValAla: 6.133 ± 0.715
0.445ValCys: 0.445 ± 0.149
5.49ValAsp: 5.49 ± 0.44
4.847ValGlu: 4.847 ± 0.555
2.127ValPhe: 2.127 ± 0.281
6.43ValGly: 6.43 ± 0.722
1.484ValHis: 1.484 ± 0.294
3.61ValIle: 3.61 ± 0.44
3.215ValLys: 3.215 ± 0.363
5.984ValLeu: 5.984 ± 0.528
1.138ValMet: 1.138 ± 0.209
2.522ValAsn: 2.522 ± 0.298
4.402ValPro: 4.402 ± 0.394
1.978ValGln: 1.978 ± 0.337
4.797ValArg: 4.797 ± 0.504
3.858ValSer: 3.858 ± 0.399
5.045ValThr: 5.045 ± 0.503
5.391ValVal: 5.391 ± 0.571
1.286ValTrp: 1.286 ± 0.252
1.533ValTyr: 1.533 ± 0.343
0.0ValXaa: 0.0 ± 0.0
Trp
1.781TrpAla: 1.781 ± 0.378
0.099TrpCys: 0.099 ± 0.061
1.632TrpAsp: 1.632 ± 0.282
1.039TrpGlu: 1.039 ± 0.249
0.495TrpPhe: 0.495 ± 0.166
1.236TrpGly: 1.236 ± 0.227
0.445TrpHis: 0.445 ± 0.149
1.039TrpIle: 1.039 ± 0.216
0.692TrpLys: 0.692 ± 0.212
1.682TrpLeu: 1.682 ± 0.326
0.346TrpMet: 0.346 ± 0.119
0.742TrpAsn: 0.742 ± 0.202
0.643TrpPro: 0.643 ± 0.179
0.643TrpGln: 0.643 ± 0.2
1.385TrpArg: 1.385 ± 0.313
1.434TrpSer: 1.434 ± 0.261
1.533TrpThr: 1.533 ± 0.32
1.533TrpVal: 1.533 ± 0.279
0.841TrpTrp: 0.841 ± 0.208
0.396TrpTyr: 0.396 ± 0.145
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.473TyrAla: 2.473 ± 0.329
0.495TyrCys: 0.495 ± 0.153
1.83TyrAsp: 1.83 ± 0.27
1.632TyrGlu: 1.632 ± 0.29
0.692TyrPhe: 0.692 ± 0.195
2.077TyrGly: 2.077 ± 0.291
0.544TyrHis: 0.544 ± 0.156
0.643TyrIle: 0.643 ± 0.156
0.643TyrLys: 0.643 ± 0.178
2.423TyrLeu: 2.423 ± 0.339
0.396TyrMet: 0.396 ± 0.135
0.89TyrAsn: 0.89 ± 0.162
2.325TyrPro: 2.325 ± 0.418
0.692TyrGln: 0.692 ± 0.161
2.423TyrArg: 2.423 ± 0.4
1.286TyrSer: 1.286 ± 0.291
1.434TyrThr: 1.434 ± 0.282
2.176TyrVal: 2.176 ± 0.366
0.692TyrTrp: 0.692 ± 0.177
0.791TyrTyr: 0.791 ± 0.19
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 96 proteins (20220 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski