Amino acid dipepetide frequency for Streptomyces phage Celia

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.179AlaAla: 15.179 ± 1.258
0.462AlaCys: 0.462 ± 0.144
6.863AlaAsp: 6.863 ± 0.841
7.787AlaGlu: 7.787 ± 0.865
2.574AlaPhe: 2.574 ± 0.412
7.457AlaGly: 7.457 ± 0.885
1.98AlaHis: 1.98 ± 0.436
3.96AlaIle: 3.96 ± 0.517
4.62AlaLys: 4.62 ± 0.656
10.295AlaLeu: 10.295 ± 1.096
3.432AlaMet: 3.432 ± 0.399
2.904AlaAsn: 2.904 ± 0.477
5.147AlaPro: 5.147 ± 0.651
3.432AlaGln: 3.432 ± 0.486
7.391AlaArg: 7.391 ± 0.819
6.071AlaSer: 6.071 ± 0.716
6.533AlaThr: 6.533 ± 0.605
9.305AlaVal: 9.305 ± 0.903
2.442AlaTrp: 2.442 ± 0.377
3.366AlaTyr: 3.366 ± 0.568
0.0AlaXaa: 0.0 ± 0.0
Cys
0.594CysAla: 0.594 ± 0.196
0.066CysCys: 0.066 ± 0.062
0.528CysAsp: 0.528 ± 0.217
0.396CysGlu: 0.396 ± 0.153
0.264CysPhe: 0.264 ± 0.153
0.924CysGly: 0.924 ± 0.262
0.198CysHis: 0.198 ± 0.117
0.33CysIle: 0.33 ± 0.118
0.132CysLys: 0.132 ± 0.104
0.528CysLeu: 0.528 ± 0.222
0.066CysMet: 0.066 ± 0.061
0.066CysAsn: 0.066 ± 0.062
0.264CysPro: 0.264 ± 0.119
0.33CysGln: 0.33 ± 0.167
1.188CysArg: 1.188 ± 0.342
0.462CysSer: 0.462 ± 0.202
0.198CysThr: 0.198 ± 0.115
0.198CysVal: 0.198 ± 0.111
0.132CysTrp: 0.132 ± 0.09
0.462CysTyr: 0.462 ± 0.158
0.0CysXaa: 0.0 ± 0.0
Asp
7.523AspAla: 7.523 ± 0.696
0.396AspCys: 0.396 ± 0.155
6.269AspAsp: 6.269 ± 0.666
6.005AspGlu: 6.005 ± 0.788
1.914AspPhe: 1.914 ± 0.336
6.995AspGly: 6.995 ± 0.697
1.65AspHis: 1.65 ± 0.351
2.904AspIle: 2.904 ± 0.389
2.574AspLys: 2.574 ± 0.371
6.335AspLeu: 6.335 ± 0.535
1.914AspMet: 1.914 ± 0.378
1.518AspAsn: 1.518 ± 0.251
2.772AspPro: 2.772 ± 0.515
2.31AspGln: 2.31 ± 0.311
3.696AspArg: 3.696 ± 0.497
3.762AspSer: 3.762 ± 0.513
3.894AspThr: 3.894 ± 0.545
3.828AspVal: 3.828 ± 0.373
1.65AspTrp: 1.65 ± 0.384
1.584AspTyr: 1.584 ± 0.289
0.0AspXaa: 0.0 ± 0.0
Glu
8.645GluAla: 8.645 ± 0.702
0.99GluCys: 0.99 ± 0.343
5.016GluAsp: 5.016 ± 0.753
4.29GluGlu: 4.29 ± 0.81
1.98GluPhe: 1.98 ± 0.355
6.269GluGly: 6.269 ± 0.832
1.716GluHis: 1.716 ± 0.389
2.574GluIle: 2.574 ± 0.44
2.244GluLys: 2.244 ± 0.396
6.731GluLeu: 6.731 ± 0.676
1.65GluMet: 1.65 ± 0.319
1.452GluAsn: 1.452 ± 0.323
2.706GluPro: 2.706 ± 0.53
2.772GluGln: 2.772 ± 0.406
5.016GluArg: 5.016 ± 0.748
3.894GluSer: 3.894 ± 0.407
3.762GluThr: 3.762 ± 0.56
4.62GluVal: 4.62 ± 0.619
1.056GluTrp: 1.056 ± 0.242
2.376GluTyr: 2.376 ± 0.355
0.0GluXaa: 0.0 ± 0.0
Phe
2.904PheAla: 2.904 ± 0.392
0.132PheCys: 0.132 ± 0.102
3.234PheAsp: 3.234 ± 0.417
1.98PheGlu: 1.98 ± 0.392
0.726PhePhe: 0.726 ± 0.191
2.508PheGly: 2.508 ± 0.396
0.66PheHis: 0.66 ± 0.208
1.32PheIle: 1.32 ± 0.371
0.99PheLys: 0.99 ± 0.271
1.848PheLeu: 1.848 ± 0.345
0.396PheMet: 0.396 ± 0.176
0.726PheAsn: 0.726 ± 0.238
1.518PhePro: 1.518 ± 0.322
1.254PheGln: 1.254 ± 0.282
1.65PheArg: 1.65 ± 0.364
1.452PheSer: 1.452 ± 0.285
2.112PheThr: 2.112 ± 0.338
2.046PheVal: 2.046 ± 0.429
0.462PheTrp: 0.462 ± 0.183
0.792PheTyr: 0.792 ± 0.184
0.0PheXaa: 0.0 ± 0.0
Gly
7.853GlyAla: 7.853 ± 0.799
0.396GlyCys: 0.396 ± 0.237
5.477GlyAsp: 5.477 ± 0.658
5.609GlyGlu: 5.609 ± 0.578
2.904GlyPhe: 2.904 ± 0.468
7.259GlyGly: 7.259 ± 0.83
1.98GlyHis: 1.98 ± 0.424
3.366GlyIle: 3.366 ± 0.463
3.828GlyLys: 3.828 ± 0.529
7.457GlyLeu: 7.457 ± 0.91
1.254GlyMet: 1.254 ± 0.299
2.442GlyAsn: 2.442 ± 0.412
4.092GlyPro: 4.092 ± 0.678
2.904GlyGln: 2.904 ± 0.483
6.137GlyArg: 6.137 ± 0.761
5.411GlySer: 5.411 ± 0.734
5.609GlyThr: 5.609 ± 0.62
6.467GlyVal: 6.467 ± 0.544
2.64GlyTrp: 2.64 ± 0.566
2.772GlyTyr: 2.772 ± 0.548
0.0GlyXaa: 0.0 ± 0.0
His
1.848HisAla: 1.848 ± 0.383
0.33HisCys: 0.33 ± 0.132
1.452HisAsp: 1.452 ± 0.293
1.32HisGlu: 1.32 ± 0.257
0.66HisPhe: 0.66 ± 0.174
2.112HisGly: 2.112 ± 0.412
0.594HisHis: 0.594 ± 0.165
0.99HisIle: 0.99 ± 0.239
0.33HisLys: 0.33 ± 0.173
1.914HisLeu: 1.914 ± 0.354
0.264HisMet: 0.264 ± 0.144
0.33HisAsn: 0.33 ± 0.127
0.99HisPro: 0.99 ± 0.259
0.462HisGln: 0.462 ± 0.205
1.254HisArg: 1.254 ± 0.321
1.386HisSer: 1.386 ± 0.367
1.518HisThr: 1.518 ± 0.329
1.386HisVal: 1.386 ± 0.44
0.726HisTrp: 0.726 ± 0.264
0.726HisTyr: 0.726 ± 0.249
0.0HisXaa: 0.0 ± 0.0
Ile
4.158IleAla: 4.158 ± 0.452
0.33IleCys: 0.33 ± 0.15
3.564IleAsp: 3.564 ± 0.418
4.158IleGlu: 4.158 ± 0.539
1.386IlePhe: 1.386 ± 0.328
3.102IleGly: 3.102 ± 0.558
0.528IleHis: 0.528 ± 0.179
1.386IleIle: 1.386 ± 0.487
1.584IleLys: 1.584 ± 0.282
2.772IleLeu: 2.772 ± 0.59
0.594IleMet: 0.594 ± 0.176
1.32IleAsn: 1.32 ± 0.369
2.244IlePro: 2.244 ± 0.338
1.188IleGln: 1.188 ± 0.288
3.102IleArg: 3.102 ± 0.363
2.904IleSer: 2.904 ± 0.55
2.508IleThr: 2.508 ± 0.349
3.102IleVal: 3.102 ± 0.462
0.66IleTrp: 0.66 ± 0.161
1.056IleTyr: 1.056 ± 0.26
0.0IleXaa: 0.0 ± 0.0
Lys
3.96LysAla: 3.96 ± 0.647
0.198LysCys: 0.198 ± 0.134
2.706LysAsp: 2.706 ± 0.394
1.122LysGlu: 1.122 ± 0.25
0.858LysPhe: 0.858 ± 0.239
3.828LysGly: 3.828 ± 0.658
0.858LysHis: 0.858 ± 0.216
1.716LysIle: 1.716 ± 0.389
2.31LysLys: 2.31 ± 0.408
3.168LysLeu: 3.168 ± 0.378
0.792LysMet: 0.792 ± 0.263
1.122LysAsn: 1.122 ± 0.308
2.64LysPro: 2.64 ± 0.558
1.056LysGln: 1.056 ± 0.188
2.904LysArg: 2.904 ± 0.466
2.046LysSer: 2.046 ± 0.481
2.442LysThr: 2.442 ± 0.445
3.036LysVal: 3.036 ± 0.601
0.528LysTrp: 0.528 ± 0.183
1.254LysTyr: 1.254 ± 0.288
0.0LysXaa: 0.0 ± 0.0
Leu
9.107LeuAla: 9.107 ± 0.772
0.396LeuCys: 0.396 ± 0.164
6.533LeuAsp: 6.533 ± 0.555
5.147LeuGlu: 5.147 ± 0.678
1.32LeuPhe: 1.32 ± 0.299
6.731LeuGly: 6.731 ± 0.997
1.65LeuHis: 1.65 ± 0.305
3.762LeuIle: 3.762 ± 0.331
3.432LeuLys: 3.432 ± 0.667
6.665LeuLeu: 6.665 ± 0.672
1.98LeuMet: 1.98 ± 0.34
3.168LeuAsn: 3.168 ± 0.386
5.213LeuPro: 5.213 ± 0.544
2.244LeuGln: 2.244 ± 0.364
5.807LeuArg: 5.807 ± 0.715
5.807LeuSer: 5.807 ± 0.733
5.675LeuThr: 5.675 ± 0.717
6.269LeuVal: 6.269 ± 0.899
1.056LeuTrp: 1.056 ± 0.299
2.046LeuTyr: 2.046 ± 0.421
0.0LeuXaa: 0.0 ± 0.0
Met
3.3MetAla: 3.3 ± 0.477
0.066MetCys: 0.066 ± 0.06
0.924MetAsp: 0.924 ± 0.243
0.594MetGlu: 0.594 ± 0.185
0.462MetPhe: 0.462 ± 0.24
1.716MetGly: 1.716 ± 0.506
0.33MetHis: 0.33 ± 0.133
0.792MetIle: 0.792 ± 0.243
0.858MetLys: 0.858 ± 0.241
1.32MetLeu: 1.32 ± 0.278
0.33MetMet: 0.33 ± 0.148
0.924MetAsn: 0.924 ± 0.208
0.99MetPro: 0.99 ± 0.242
0.792MetGln: 0.792 ± 0.201
1.122MetArg: 1.122 ± 0.29
2.112MetSer: 2.112 ± 0.395
2.244MetThr: 2.244 ± 0.331
1.32MetVal: 1.32 ± 0.275
0.132MetTrp: 0.132 ± 0.089
0.462MetTyr: 0.462 ± 0.196
0.0MetXaa: 0.0 ± 0.0
Asn
2.772AsnAla: 2.772 ± 0.499
0.198AsnCys: 0.198 ± 0.109
1.122AsnAsp: 1.122 ± 0.276
2.772AsnGlu: 2.772 ± 0.349
0.858AsnPhe: 0.858 ± 0.27
3.168AsnGly: 3.168 ± 0.382
0.528AsnHis: 0.528 ± 0.203
0.99AsnIle: 0.99 ± 0.234
1.188AsnLys: 1.188 ± 0.213
2.31AsnLeu: 2.31 ± 0.377
0.396AsnMet: 0.396 ± 0.141
0.726AsnAsn: 0.726 ± 0.223
1.782AsnPro: 1.782 ± 0.314
0.594AsnGln: 0.594 ± 0.204
2.046AsnArg: 2.046 ± 0.335
1.122AsnSer: 1.122 ± 0.227
1.782AsnThr: 1.782 ± 0.445
2.112AsnVal: 2.112 ± 0.425
0.528AsnTrp: 0.528 ± 0.192
0.924AsnTyr: 0.924 ± 0.224
0.0AsnXaa: 0.0 ± 0.0
Pro
6.533ProAla: 6.533 ± 0.886
0.528ProCys: 0.528 ± 0.19
3.366ProAsp: 3.366 ± 0.391
4.422ProGlu: 4.422 ± 0.577
1.122ProPhe: 1.122 ± 0.268
4.62ProGly: 4.62 ± 0.61
0.726ProHis: 0.726 ± 0.198
2.112ProIle: 2.112 ± 0.481
1.98ProLys: 1.98 ± 0.465
3.564ProLeu: 3.564 ± 0.525
0.726ProMet: 0.726 ± 0.187
1.122ProAsn: 1.122 ± 0.297
2.244ProPro: 2.244 ± 0.501
1.914ProGln: 1.914 ± 0.386
2.442ProArg: 2.442 ± 0.402
3.168ProSer: 3.168 ± 0.599
3.762ProThr: 3.762 ± 0.708
3.564ProVal: 3.564 ± 0.623
0.924ProTrp: 0.924 ± 0.235
0.924ProTyr: 0.924 ± 0.26
0.0ProXaa: 0.0 ± 0.0
Gln
3.762GlnAla: 3.762 ± 0.499
0.198GlnCys: 0.198 ± 0.105
1.254GlnAsp: 1.254 ± 0.284
2.046GlnGlu: 2.046 ± 0.359
1.386GlnPhe: 1.386 ± 0.215
2.376GlnGly: 2.376 ± 0.389
0.462GlnHis: 0.462 ± 0.241
1.386GlnIle: 1.386 ± 0.231
0.858GlnLys: 0.858 ± 0.311
2.64GlnLeu: 2.64 ± 0.562
1.056GlnMet: 1.056 ± 0.235
0.99GlnAsn: 0.99 ± 0.235
1.254GlnPro: 1.254 ± 0.354
0.792GlnGln: 0.792 ± 0.193
3.168GlnArg: 3.168 ± 0.536
1.32GlnSer: 1.32 ± 0.299
2.112GlnThr: 2.112 ± 0.334
3.3GlnVal: 3.3 ± 0.411
0.396GlnTrp: 0.396 ± 0.122
0.99GlnTyr: 0.99 ± 0.257
0.0GlnXaa: 0.0 ± 0.0
Arg
7.325ArgAla: 7.325 ± 0.764
0.924ArgCys: 0.924 ± 0.292
4.224ArgAsp: 4.224 ± 0.523
4.686ArgGlu: 4.686 ± 0.727
2.838ArgPhe: 2.838 ± 0.418
5.477ArgGly: 5.477 ± 0.681
1.584ArgHis: 1.584 ± 0.355
3.96ArgIle: 3.96 ± 0.592
3.036ArgLys: 3.036 ± 0.498
6.005ArgLeu: 6.005 ± 0.622
1.056ArgMet: 1.056 ± 0.246
1.65ArgAsn: 1.65 ± 0.397
2.838ArgPro: 2.838 ± 0.472
2.574ArgGln: 2.574 ± 0.507
6.665ArgArg: 6.665 ± 0.877
4.488ArgSer: 4.488 ± 0.65
4.026ArgThr: 4.026 ± 0.567
4.224ArgVal: 4.224 ± 0.536
0.726ArgTrp: 0.726 ± 0.248
2.244ArgTyr: 2.244 ± 0.384
0.0ArgXaa: 0.0 ± 0.0
Ser
7.061SerAla: 7.061 ± 0.67
0.198SerCys: 0.198 ± 0.126
3.828SerAsp: 3.828 ± 0.452
3.696SerGlu: 3.696 ± 0.585
1.782SerPhe: 1.782 ± 0.327
5.741SerGly: 5.741 ± 0.633
1.65SerHis: 1.65 ± 0.442
2.442SerIle: 2.442 ± 0.455
1.584SerLys: 1.584 ± 0.321
5.279SerLeu: 5.279 ± 0.742
1.386SerMet: 1.386 ± 0.267
1.914SerAsn: 1.914 ± 0.428
2.64SerPro: 2.64 ± 0.423
1.716SerGln: 1.716 ± 0.362
3.96SerArg: 3.96 ± 0.617
3.564SerSer: 3.564 ± 0.565
4.092SerThr: 4.092 ± 0.675
3.696SerVal: 3.696 ± 0.565
1.716SerTrp: 1.716 ± 0.378
1.452SerTyr: 1.452 ± 0.27
0.0SerXaa: 0.0 ± 0.0
Thr
6.137ThrAla: 6.137 ± 0.556
0.33ThrCys: 0.33 ± 0.151
4.488ThrAsp: 4.488 ± 0.578
5.213ThrGlu: 5.213 ± 0.814
2.376ThrPhe: 2.376 ± 0.485
5.873ThrGly: 5.873 ± 0.9
1.584ThrHis: 1.584 ± 0.329
3.036ThrIle: 3.036 ± 0.428
2.178ThrLys: 2.178 ± 0.489
5.477ThrLeu: 5.477 ± 0.738
0.924ThrMet: 0.924 ± 0.285
1.782ThrAsn: 1.782 ± 0.369
4.29ThrPro: 4.29 ± 0.539
1.518ThrGln: 1.518 ± 0.274
3.564ThrArg: 3.564 ± 0.427
3.63ThrSer: 3.63 ± 0.513
3.96ThrThr: 3.96 ± 0.519
4.62ThrVal: 4.62 ± 0.621
1.188ThrTrp: 1.188 ± 0.313
1.914ThrTyr: 1.914 ± 0.36
0.0ThrXaa: 0.0 ± 0.0
Val
7.853ValAla: 7.853 ± 0.7
0.66ValCys: 0.66 ± 0.223
4.752ValAsp: 4.752 ± 0.489
5.147ValGlu: 5.147 ± 0.569
1.98ValPhe: 1.98 ± 0.312
4.818ValGly: 4.818 ± 0.602
1.056ValHis: 1.056 ± 0.287
3.498ValIle: 3.498 ± 0.38
3.102ValLys: 3.102 ± 0.509
6.137ValLeu: 6.137 ± 0.754
1.32ValMet: 1.32 ± 0.288
2.31ValAsn: 2.31 ± 0.386
3.96ValPro: 3.96 ± 0.537
2.31ValGln: 2.31 ± 0.458
5.345ValArg: 5.345 ± 0.548
4.092ValSer: 4.092 ± 0.472
5.082ValThr: 5.082 ± 0.539
5.411ValVal: 5.411 ± 0.58
1.386ValTrp: 1.386 ± 0.289
1.386ValTyr: 1.386 ± 0.294
0.0ValXaa: 0.0 ± 0.0
Trp
2.178TrpAla: 2.178 ± 0.345
0.396TrpCys: 0.396 ± 0.147
1.518TrpAsp: 1.518 ± 0.318
1.188TrpGlu: 1.188 ± 0.297
0.528TrpPhe: 0.528 ± 0.173
1.782TrpGly: 1.782 ± 0.308
0.462TrpHis: 0.462 ± 0.209
0.462TrpIle: 0.462 ± 0.17
0.924TrpLys: 0.924 ± 0.238
1.452TrpLeu: 1.452 ± 0.316
0.33TrpMet: 0.33 ± 0.123
0.66TrpAsn: 0.66 ± 0.265
0.528TrpPro: 0.528 ± 0.182
0.66TrpGln: 0.66 ± 0.204
1.782TrpArg: 1.782 ± 0.426
1.254TrpSer: 1.254 ± 0.295
1.32TrpThr: 1.32 ± 0.26
1.188TrpVal: 1.188 ± 0.292
0.33TrpTrp: 0.33 ± 0.17
0.33TrpTyr: 0.33 ± 0.153
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.574TyrAla: 2.574 ± 0.393
0.132TyrCys: 0.132 ± 0.097
2.376TyrAsp: 2.376 ± 0.374
2.178TyrGlu: 2.178 ± 0.475
0.792TyrPhe: 0.792 ± 0.201
3.102TyrGly: 3.102 ± 0.542
0.396TyrHis: 0.396 ± 0.189
0.66TyrIle: 0.66 ± 0.201
0.792TyrLys: 0.792 ± 0.223
2.112TyrLeu: 2.112 ± 0.382
0.858TyrMet: 0.858 ± 0.218
0.792TyrAsn: 0.792 ± 0.208
1.65TyrPro: 1.65 ± 0.335
0.924TyrGln: 0.924 ± 0.254
2.31TyrArg: 2.31 ± 0.365
1.452TyrSer: 1.452 ± 0.314
1.452TyrThr: 1.452 ± 0.228
1.914TyrVal: 1.914 ± 0.342
0.594TyrTrp: 0.594 ± 0.193
0.726TyrTyr: 0.726 ± 0.174
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 77 proteins (15154 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski