Musca hytrovirus(isolate Musca domestica/United States/Boucias/-) (MHV)

Taxonomy: Viruses; Naldaviricetes; Lefavirales; Hytrosaviridae; Muscavirus; Musca hytrosavirus

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 108 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B2YG26|B2YG26_MHVB Ac150-like protein OS=Musca hytrovirus(isolate Musca domestica/United States/Boucias/-) OX=523909 GN=MdSGHV049 PE=4 SV=1
MM1 pKa = 7.3SRR3 pKa = 11.84NFAFFLFLGICLVASRR19 pKa = 11.84VVDD22 pKa = 3.95CQEE25 pKa = 3.74GTEE28 pKa = 4.03DD29 pKa = 3.27QTVNEE34 pKa = 4.42TQGDD38 pKa = 3.66QSVNEE43 pKa = 4.14NPGDD47 pKa = 3.71QSVDD51 pKa = 3.27EE52 pKa = 4.52AQGDD56 pKa = 3.64QSVNEE61 pKa = 4.14NPGDD65 pKa = 3.59QSVYY69 pKa = 9.96EE70 pKa = 4.13AQGDD74 pKa = 3.72QSVNEE79 pKa = 4.19VPEE82 pKa = 4.4DD83 pKa = 3.71QPADD87 pKa = 3.79DD88 pKa = 4.5VADD91 pKa = 4.48PVPDD95 pKa = 4.43DD96 pKa = 4.13QPADD100 pKa = 3.82DD101 pKa = 4.83VGSEE105 pKa = 4.39STNSPHH111 pKa = 7.66EE112 pKa = 3.86ITEE115 pKa = 4.22PDD117 pKa = 3.23ASGDD121 pKa = 3.76GQASEE126 pKa = 4.68DD127 pKa = 3.5QSAPTEE133 pKa = 4.2APGDD137 pKa = 3.52QSAPTEE143 pKa = 4.28APGDD147 pKa = 3.88QPVDD151 pKa = 3.64EE152 pKa = 5.28APGASEE158 pKa = 3.97NEE160 pKa = 4.1KK161 pKa = 10.72KK162 pKa = 10.71EE163 pKa = 4.12EE164 pKa = 4.05EE165 pKa = 4.19EE166 pKa = 4.28EE167 pKa = 4.17PAVVPDD173 pKa = 4.03GDD175 pKa = 4.23GSGDD179 pKa = 3.52VNNDD183 pKa = 3.38SNHH186 pKa = 6.07SNSTEE191 pKa = 3.95VAVEE195 pKa = 4.2DD196 pKa = 4.62NSDD199 pKa = 3.63HH200 pKa = 8.12VDD202 pKa = 3.59DD203 pKa = 4.86QSEE206 pKa = 4.31NNPGGRR212 pKa = 11.84DD213 pKa = 3.62EE214 pKa = 5.31VPTEE218 pKa = 4.28SPPEE222 pKa = 4.11DD223 pKa = 3.06TSAPEE228 pKa = 4.15NIEE231 pKa = 4.12DD232 pKa = 4.27EE233 pKa = 4.27KK234 pKa = 11.47SKK236 pKa = 11.25YY237 pKa = 10.65LGDD240 pKa = 3.6EE241 pKa = 4.23YY242 pKa = 11.36NFFMEE247 pKa = 4.55HH248 pKa = 6.83CSPSEE253 pKa = 4.19SVNHH257 pKa = 6.66FGYY260 pKa = 10.8EE261 pKa = 3.72LLYY264 pKa = 11.1DD265 pKa = 3.64FFINVLHH272 pKa = 6.92IFYY275 pKa = 10.24SDD277 pKa = 3.51HH278 pKa = 7.38ALDD281 pKa = 5.04GYY283 pKa = 11.12NSNRR287 pKa = 11.84TIQKK291 pKa = 8.74YY292 pKa = 9.81FGRR295 pKa = 11.84DD296 pKa = 2.9VYY298 pKa = 11.5DD299 pKa = 3.42QVFNDD304 pKa = 3.57YY305 pKa = 11.53ANAHH309 pKa = 6.03SLSEE313 pKa = 4.11PLMSCPEE320 pKa = 3.87NVEE323 pKa = 4.26EE324 pKa = 5.13KK325 pKa = 10.34FKK327 pKa = 11.33KK328 pKa = 10.33SINEE332 pKa = 4.45FIVHH336 pKa = 6.95ALSTLLTIYY345 pKa = 10.93YY346 pKa = 9.2YY347 pKa = 10.45GTVSADD353 pKa = 3.45DD354 pKa = 3.41IQRR357 pKa = 11.84IEE359 pKa = 4.14EE360 pKa = 4.23TLSAGQQIDD369 pKa = 3.89SLIDD373 pKa = 3.63GYY375 pKa = 11.62GQMSGVLMDD384 pKa = 4.49LCRR387 pKa = 11.84KK388 pKa = 9.17CHH390 pKa = 5.73

Molecular weight: 42.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B2YG04|B2YG04_MHVB PDDEXK_1 domain-containing protein OS=Musca hytrovirus(isolate Musca domestica/United States/Boucias/-) OX=523909 GN=MdSGHV027 PE=4 SV=1
MM1 pKa = 7.7AKK3 pKa = 10.44ASLDD7 pKa = 3.81SISRR11 pKa = 11.84NMRR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84KK17 pKa = 9.61RR18 pKa = 11.84KK19 pKa = 8.97SSSSSSSSSSSSSKK33 pKa = 10.11RR34 pKa = 11.84RR35 pKa = 11.84TPRR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.43SSSKK44 pKa = 10.2RR45 pKa = 11.84RR46 pKa = 11.84TTTRR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84APAYY58 pKa = 10.13RR59 pKa = 11.84RR60 pKa = 3.57

Molecular weight: 7.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

108

0

108

37570

51

1780

347.9

39.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.961 ± 0.147

2.036 ± 0.142

6.42 ± 0.207

5.193 ± 0.162

4.847 ± 0.138

4.357 ± 0.201

2.31 ± 0.125

6.939 ± 0.154

4.884 ± 0.184

9.084 ± 0.233

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.05 ± 0.107

6.537 ± 0.207

4.064 ± 0.167

3.7 ± 0.111

6.114 ± 0.208

7.49 ± 0.217

6.268 ± 0.188

6.22 ± 0.142

0.735 ± 0.053

4.788 ± 0.162
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski