Brachyspira sp. CAG:484

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Brachyspirales; Brachyspiraceae; Brachyspira; environmental samples

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2216 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5HAK2|R5HAK2_9SPIR Diaminopimelate epimerase OS=Brachyspira sp. CAG:484 OX=1262759 GN=dapF PE=3 SV=1
MM1 pKa = 7.69IDD3 pKa = 3.43YY4 pKa = 8.76TKK6 pKa = 11.12EE7 pKa = 3.78EE8 pKa = 4.28LVEE11 pKa = 4.09LLRR14 pKa = 11.84KK15 pKa = 9.9HH16 pKa = 5.91PEE18 pKa = 3.91KK19 pKa = 10.7FNEE22 pKa = 3.68WKK24 pKa = 10.8YY25 pKa = 9.26EE26 pKa = 3.88QEE28 pKa = 4.11EE29 pKa = 4.38VDD31 pKa = 3.79LSEE34 pKa = 4.89VDD36 pKa = 3.85FSGINLSEE44 pKa = 3.99IDD46 pKa = 4.52FSDD49 pKa = 3.23VDD51 pKa = 4.5LNSSSFADD59 pKa = 3.58CVLSFVKK66 pKa = 10.51FNGADD71 pKa = 3.42LTSVDD76 pKa = 3.89FTRR79 pKa = 11.84AKK81 pKa = 10.2IIEE84 pKa = 4.31CDD86 pKa = 3.54FTEE89 pKa = 4.36SLLTGTDD96 pKa = 3.07CSYY99 pKa = 12.08AEE101 pKa = 3.73MTYY104 pKa = 11.15CNFTDD109 pKa = 4.72ADD111 pKa = 4.03LAGCIFSEE119 pKa = 4.4SDD121 pKa = 3.29LSNSDD126 pKa = 3.64FSSSYY131 pKa = 10.58NLHH134 pKa = 6.4ACRR137 pKa = 11.84FDD139 pKa = 5.07EE140 pKa = 4.31DD141 pKa = 4.87TIWPDD146 pKa = 3.9SEE148 pKa = 4.41YY149 pKa = 11.27LPDD152 pKa = 5.94DD153 pKa = 4.24FDD155 pKa = 5.73ASYY158 pKa = 11.58KK159 pKa = 10.72DD160 pKa = 4.24DD161 pKa = 4.41LSSLKK166 pKa = 10.86DD167 pKa = 3.39EE168 pKa = 4.41EE169 pKa = 5.63DD170 pKa = 3.89YY171 pKa = 11.86GNSDD175 pKa = 3.58YY176 pKa = 11.92

Molecular weight: 20.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5H6V2|R5H6V2_9SPIR General secretion pathway protein G OS=Brachyspira sp. CAG:484 OX=1262759 GN=BN676_01790 PE=4 SV=1
MM1 pKa = 7.68RR2 pKa = 11.84RR3 pKa = 11.84TLEE6 pKa = 3.63GTKK9 pKa = 9.62IKK11 pKa = 9.84RR12 pKa = 11.84QHH14 pKa = 5.59VSGFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84EE29 pKa = 3.48VLNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84HH40 pKa = 5.21KK41 pKa = 10.78LAITAKK47 pKa = 10.06KK48 pKa = 9.87RR49 pKa = 11.84AA50 pKa = 3.6

Molecular weight: 5.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2216

0

2216

652095

29

5280

294.3

33.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.03 ± 0.055

1.311 ± 0.026

5.676 ± 0.04

6.946 ± 0.058

4.482 ± 0.049

6.091 ± 0.058

1.481 ± 0.019

8.088 ± 0.051

8.883 ± 0.057

8.903 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.525 ± 0.028

6.143 ± 0.08

3.4 ± 0.044

3.223 ± 0.037

3.504 ± 0.038

6.047 ± 0.059

5.376 ± 0.043

6.049 ± 0.051

0.733 ± 0.015

4.107 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski