Kemerovo virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Orbivirus; Great Island virus

Average proteome isoelectric point is 7.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4MJR4|M4MJR4_9REOV Non-structural protein NS2 OS=Kemerovo virus OX=40064 GN=NS2 PE=4 SV=1
MM1 pKa = 7.61SSDD4 pKa = 3.35KK5 pKa = 11.22QNVPMIKK12 pKa = 10.12KK13 pKa = 8.89PFTRR17 pKa = 11.84VIVLKK22 pKa = 10.83SDD24 pKa = 3.09GRR26 pKa = 11.84DD27 pKa = 3.28DD28 pKa = 5.59FVAKK32 pKa = 9.62MCAVLGCEE40 pKa = 4.12YY41 pKa = 10.94VVVRR45 pKa = 11.84HH46 pKa = 6.02GLGTQIAGSDD56 pKa = 3.92TPAHH60 pKa = 6.21GCLLLTIPGPGSFRR74 pKa = 11.84MIDD77 pKa = 3.36RR78 pKa = 11.84DD79 pKa = 3.26QSTFLVVSGDD89 pKa = 3.62GLEE92 pKa = 4.21VQQDD96 pKa = 3.09RR97 pKa = 11.84WTGMRR102 pKa = 11.84FEE104 pKa = 4.77AVDD107 pKa = 3.67MYY109 pKa = 11.12PRR111 pKa = 11.84ACRR114 pKa = 11.84IRR116 pKa = 11.84YY117 pKa = 8.79GEE119 pKa = 4.44KK120 pKa = 10.66EE121 pKa = 3.91NDD123 pKa = 3.08SEE125 pKa = 4.39IRR127 pKa = 11.84FGRR130 pKa = 11.84ASGTVPPYY138 pKa = 10.31TPDD141 pKa = 3.3GVSEE145 pKa = 4.11EE146 pKa = 4.19QEE148 pKa = 4.18EE149 pKa = 4.53VALPGISFHH158 pKa = 6.26TPEE161 pKa = 4.57EE162 pKa = 4.42DD163 pKa = 3.3YY164 pKa = 11.35KK165 pKa = 11.01EE166 pKa = 3.86YY167 pKa = 10.64RR168 pKa = 11.84EE169 pKa = 4.02RR170 pKa = 11.84LRR172 pKa = 11.84EE173 pKa = 3.94EE174 pKa = 3.92RR175 pKa = 11.84DD176 pKa = 3.35LRR178 pKa = 11.84SANILEE184 pKa = 4.44ALRR187 pKa = 11.84TTHH190 pKa = 6.65QSRR193 pKa = 11.84TLGRR197 pKa = 11.84IHH199 pKa = 7.93GIRR202 pKa = 11.84NSEE205 pKa = 4.21LTTLRR210 pKa = 11.84PPAPPTLRR218 pKa = 11.84RR219 pKa = 11.84GVSSSNEE226 pKa = 3.48RR227 pKa = 11.84SADD230 pKa = 3.9RR231 pKa = 11.84TPTPPKK237 pKa = 9.37LTAPTYY243 pKa = 7.38PTAAAPRR250 pKa = 11.84AAAAPPPIPSVKK262 pKa = 9.75RR263 pKa = 11.84VSKK266 pKa = 8.77TAPQEE271 pKa = 4.54AEE273 pKa = 4.11SQCPKK278 pKa = 9.5FTHH281 pKa = 6.85EE282 pKa = 3.99YY283 pKa = 10.19DD284 pKa = 3.51QLAEE288 pKa = 4.06RR289 pKa = 11.84AFEE292 pKa = 4.77SLLEE296 pKa = 4.62DD297 pKa = 4.2DD298 pKa = 4.14PNARR302 pKa = 11.84FSYY305 pKa = 10.14GGAPEE310 pKa = 4.2TVGLFSQCLGTYY322 pKa = 9.44EE323 pKa = 4.66VDD325 pKa = 3.52PLMLPAYY332 pKa = 9.87EE333 pKa = 4.48IDD335 pKa = 3.55QTKK338 pKa = 10.21NRR340 pKa = 11.84YY341 pKa = 9.37KK342 pKa = 10.14YY343 pKa = 11.06VGMKK347 pKa = 9.74TSSQLNALVCDD358 pKa = 3.73DD359 pKa = 3.65RR360 pKa = 11.84VYY362 pKa = 10.72FVPSANN368 pKa = 3.3

Molecular weight: 40.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4MBL5|M4MBL5_9REOV RNA-directed RNA polymerase OS=Kemerovo virus OX=40064 GN=VP1 PE=3 SV=1
MM1 pKa = 7.47LAALEE6 pKa = 4.48MKK8 pKa = 10.27QSPTAPPAYY17 pKa = 9.92AATPMANVALGVLQNAITSTTGANEE42 pKa = 3.67TARR45 pKa = 11.84NEE47 pKa = 3.88KK48 pKa = 9.91AAYY51 pKa = 8.47GAAVEE56 pKa = 4.23ALKK59 pKa = 10.8DD60 pKa = 3.81DD61 pKa = 3.75EE62 pKa = 4.51TTRR65 pKa = 11.84MLKK68 pKa = 10.32AHH70 pKa = 5.8VNEE73 pKa = 4.24TSLRR77 pKa = 11.84EE78 pKa = 4.19AEE80 pKa = 4.02RR81 pKa = 11.84TLAKK85 pKa = 10.37LKK87 pKa = 10.52RR88 pKa = 11.84KK89 pKa = 9.6CLLLRR94 pKa = 11.84CAEE97 pKa = 4.17LMCLALTIGISVVLMITSAADD118 pKa = 3.19ALEE121 pKa = 4.38AQLKK125 pKa = 8.58RR126 pKa = 11.84INMSSHH132 pKa = 6.85VITGLLTAAAIGLQRR147 pKa = 11.84ARR149 pKa = 11.84QSHH152 pKa = 4.05QRR154 pKa = 11.84RR155 pKa = 11.84KK156 pKa = 10.34KK157 pKa = 9.26SLKK160 pKa = 9.23RR161 pKa = 11.84DD162 pKa = 3.55LVKK165 pKa = 10.82KK166 pKa = 10.0QMYY169 pKa = 8.86VSFVRR174 pKa = 11.84RR175 pKa = 11.84MGSQIPEE182 pKa = 3.96SSAAGPDD189 pKa = 3.23FHH191 pKa = 8.25ARR193 pKa = 11.84LLALAEE199 pKa = 3.73EE200 pKa = 4.62AGRR203 pKa = 11.84HH204 pKa = 5.48KK205 pKa = 10.04EE206 pKa = 4.16TSDD209 pKa = 2.83WRR211 pKa = 11.84HH212 pKa = 4.71WPP214 pKa = 3.35

Molecular weight: 23.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

5701

214

1285

570.1

64.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.963 ± 0.627

1.421 ± 0.312

5.911 ± 0.421

6.209 ± 0.363

3.491 ± 0.269

5.736 ± 0.328

3.298 ± 0.284

5.017 ± 0.379

2.842 ± 0.501

9.174 ± 0.392

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.561 ± 0.216

2.789 ± 0.145

5.297 ± 0.499

3.508 ± 0.431

9.577 ± 0.234

6.122 ± 0.27

6.157 ± 0.383

6.771 ± 0.214

1.052 ± 0.188

3.105 ± 0.269
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski