Amino acid dipepetide frequency for Streptomyces sp. HG99

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.172AlaAla: 20.172 ± 0.165
1.062AlaCys: 1.062 ± 0.024
8.232AlaAsp: 8.232 ± 0.061
8.856AlaGlu: 8.856 ± 0.098
3.435AlaPhe: 3.435 ± 0.042
11.812AlaGly: 11.812 ± 0.09
2.891AlaHis: 2.891 ± 0.038
3.717AlaIle: 3.717 ± 0.048
3.221AlaLys: 3.221 ± 0.049
13.769AlaLeu: 13.769 ± 0.111
2.575AlaMet: 2.575 ± 0.035
2.043AlaAsn: 2.043 ± 0.031
6.885AlaPro: 6.885 ± 0.081
4.112AlaGln: 4.112 ± 0.048
9.915AlaArg: 9.915 ± 0.083
6.223AlaSer: 6.223 ± 0.058
6.879AlaThr: 6.879 ± 0.067
11.566AlaVal: 11.566 ± 0.096
1.911AlaTrp: 1.911 ± 0.033
2.866AlaTyr: 2.866 ± 0.038
0.0AlaXaa: 0.0 ± 0.0
Cys
1.073CysAla: 1.073 ± 0.023
0.101CysCys: 0.101 ± 0.008
0.473CysAsp: 0.473 ± 0.015
0.435CysGlu: 0.435 ± 0.015
0.22CysPhe: 0.22 ± 0.011
0.927CysGly: 0.927 ± 0.022
0.201CysHis: 0.201 ± 0.009
0.18CysIle: 0.18 ± 0.009
0.13CysLys: 0.13 ± 0.009
0.733CysLeu: 0.733 ± 0.02
0.124CysMet: 0.124 ± 0.008
0.136CysAsn: 0.136 ± 0.007
0.521CysPro: 0.521 ± 0.015
0.179CysGln: 0.179 ± 0.01
0.619CysArg: 0.619 ± 0.017
0.452CysSer: 0.452 ± 0.014
0.548CysThr: 0.548 ± 0.017
0.638CysVal: 0.638 ± 0.016
0.136CysTrp: 0.136 ± 0.007
0.157CysTyr: 0.157 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
7.668AspAla: 7.668 ± 0.066
0.446AspCys: 0.446 ± 0.014
3.794AspAsp: 3.794 ± 0.049
4.122AspGlu: 4.122 ± 0.05
1.736AspPhe: 1.736 ± 0.028
6.234AspGly: 6.234 ± 0.062
1.449AspHis: 1.449 ± 0.029
2.066AspIle: 2.066 ± 0.033
1.401AspLys: 1.401 ± 0.031
6.2AspLeu: 6.2 ± 0.056
0.886AspMet: 0.886 ± 0.02
1.053AspAsn: 1.053 ± 0.022
4.446AspPro: 4.446 ± 0.047
1.721AspGln: 1.721 ± 0.027
4.742AspArg: 4.742 ± 0.054
2.699AspSer: 2.699 ± 0.038
3.233AspThr: 3.233 ± 0.036
4.773AspVal: 4.773 ± 0.049
1.059AspTrp: 1.059 ± 0.022
1.16AspTyr: 1.16 ± 0.022
0.0AspXaa: 0.0 ± 0.0
Glu
7.562GluAla: 7.562 ± 0.067
0.374GluCys: 0.374 ± 0.012
2.956GluAsp: 2.956 ± 0.036
3.63GluGlu: 3.63 ± 0.057
1.528GluPhe: 1.528 ± 0.028
4.487GluGly: 4.487 ± 0.049
1.588GluHis: 1.588 ± 0.029
2.42GluIle: 2.42 ± 0.035
1.582GluLys: 1.582 ± 0.031
6.972GluLeu: 6.972 ± 0.064
0.927GluMet: 0.927 ± 0.02
1.153GluAsn: 1.153 ± 0.025
3.454GluPro: 3.454 ± 0.042
2.429GluGln: 2.429 ± 0.035
5.551GluArg: 5.551 ± 0.058
2.716GluSer: 2.716 ± 0.035
3.03GluThr: 3.03 ± 0.04
4.411GluVal: 4.411 ± 0.049
0.851GluTrp: 0.851 ± 0.02
1.174GluTyr: 1.174 ± 0.023
0.0GluXaa: 0.0 ± 0.0
Phe
3.591PheAla: 3.591 ± 0.039
0.263PheCys: 0.263 ± 0.01
1.938PheAsp: 1.938 ± 0.032
1.488PheGlu: 1.488 ± 0.027
0.832PhePhe: 0.832 ± 0.022
2.977PheGly: 2.977 ± 0.04
0.608PheHis: 0.608 ± 0.016
0.798PheIle: 0.798 ± 0.021
0.581PheLys: 0.581 ± 0.019
2.533PheLeu: 2.533 ± 0.038
0.413PheMet: 0.413 ± 0.014
0.586PheAsn: 0.586 ± 0.019
1.421PhePro: 1.421 ± 0.025
0.688PheGln: 0.688 ± 0.015
1.791PheArg: 1.791 ± 0.028
1.435PheSer: 1.435 ± 0.025
1.99PheThr: 1.99 ± 0.032
2.203PheVal: 2.203 ± 0.032
0.406PheTrp: 0.406 ± 0.015
0.579PheTyr: 0.579 ± 0.016
0.0PheXaa: 0.0 ± 0.0
Gly
10.443GlyAla: 10.443 ± 0.091
0.822GlyCys: 0.822 ± 0.019
5.179GlyAsp: 5.179 ± 0.053
5.02GlyGlu: 5.02 ± 0.045
2.814GlyPhe: 2.814 ± 0.041
8.309GlyGly: 8.309 ± 0.088
2.339GlyHis: 2.339 ± 0.035
3.59GlyIle: 3.59 ± 0.045
2.611GlyLys: 2.611 ± 0.045
9.061GlyLeu: 9.061 ± 0.072
1.944GlyMet: 1.944 ± 0.03
1.854GlyAsn: 1.854 ± 0.036
4.883GlyPro: 4.883 ± 0.055
2.833GlyGln: 2.833 ± 0.042
7.337GlyArg: 7.337 ± 0.07
5.325GlySer: 5.325 ± 0.05
6.014GlyThr: 6.014 ± 0.063
7.152GlyVal: 7.152 ± 0.054
1.708GlyTrp: 1.708 ± 0.028
2.222GlyTyr: 2.222 ± 0.03
0.0GlyXaa: 0.0 ± 0.0
His
2.732HisAla: 2.732 ± 0.043
0.214HisCys: 0.214 ± 0.01
1.399HisAsp: 1.399 ± 0.025
1.299HisGlu: 1.299 ± 0.024
0.67HisPhe: 0.67 ± 0.018
2.403HisGly: 2.403 ± 0.035
0.716HisHis: 0.716 ± 0.023
0.763HisIle: 0.763 ± 0.02
0.407HisLys: 0.407 ± 0.012
2.447HisLeu: 2.447 ± 0.033
0.359HisMet: 0.359 ± 0.013
0.412HisAsn: 0.412 ± 0.013
1.822HisPro: 1.822 ± 0.028
0.716HisGln: 0.716 ± 0.018
2.094HisArg: 2.094 ± 0.034
1.057HisSer: 1.057 ± 0.02
1.369HisThr: 1.369 ± 0.024
1.733HisVal: 1.733 ± 0.028
0.371HisTrp: 0.371 ± 0.013
0.497HisTyr: 0.497 ± 0.015
0.0HisXaa: 0.0 ± 0.0
Ile
5.024IleAla: 5.024 ± 0.053
0.308IleCys: 0.308 ± 0.01
2.439IleAsp: 2.439 ± 0.035
2.185IleGlu: 2.185 ± 0.033
0.734IlePhe: 0.734 ± 0.017
3.618IleGly: 3.618 ± 0.037
0.684IleHis: 0.684 ± 0.016
0.951IleIle: 0.951 ± 0.025
0.885IleLys: 0.885 ± 0.023
2.558IleLeu: 2.558 ± 0.036
0.453IleMet: 0.453 ± 0.014
0.76IleAsn: 0.76 ± 0.019
1.937IlePro: 1.937 ± 0.03
0.829IleGln: 0.829 ± 0.018
2.383IleArg: 2.383 ± 0.034
1.783IleSer: 1.783 ± 0.028
2.374IleThr: 2.374 ± 0.037
2.862IleVal: 2.862 ± 0.038
0.396IleTrp: 0.396 ± 0.013
0.6IleTyr: 0.6 ± 0.017
0.0IleXaa: 0.0 ± 0.0
Lys
3.327LysAla: 3.327 ± 0.05
0.133LysCys: 0.133 ± 0.008
1.528LysAsp: 1.528 ± 0.032
1.362LysGlu: 1.362 ± 0.029
0.516LysPhe: 0.516 ± 0.017
2.057LysGly: 2.057 ± 0.039
0.485LysHis: 0.485 ± 0.016
0.986LysIle: 0.986 ± 0.025
1.011LysLys: 1.011 ± 0.033
2.249LysLeu: 2.249 ± 0.035
0.438LysMet: 0.438 ± 0.015
0.616LysAsn: 0.616 ± 0.017
1.523LysPro: 1.523 ± 0.031
0.779LysGln: 0.779 ± 0.023
1.631LysArg: 1.631 ± 0.031
1.305LysSer: 1.305 ± 0.027
1.516LysThr: 1.516 ± 0.03
2.046LysVal: 2.046 ± 0.044
0.3LysTrp: 0.3 ± 0.011
0.508LysTyr: 0.508 ± 0.015
0.0LysXaa: 0.0 ± 0.0
Leu
14.423LeuAla: 14.423 ± 0.102
0.82LeuCys: 0.82 ± 0.019
6.688LeuAsp: 6.688 ± 0.074
4.844LeuGlu: 4.844 ± 0.051
2.518LeuPhe: 2.518 ± 0.034
8.797LeuGly: 8.797 ± 0.064
2.286LeuHis: 2.286 ± 0.035
3.482LeuIle: 3.482 ± 0.038
2.311LeuLys: 2.311 ± 0.041
10.71LeuLeu: 10.71 ± 0.101
1.674LeuMet: 1.674 ± 0.028
1.837LeuAsn: 1.837 ± 0.028
6.346LeuPro: 6.346 ± 0.065
2.355LeuGln: 2.355 ± 0.032
8.387LeuArg: 8.387 ± 0.077
5.359LeuSer: 5.359 ± 0.054
6.732LeuThr: 6.732 ± 0.058
8.365LeuVal: 8.365 ± 0.069
1.298LeuTrp: 1.298 ± 0.025
1.89LeuTyr: 1.89 ± 0.029
0.0LeuXaa: 0.0 ± 0.0
Met
2.36MetAla: 2.36 ± 0.034
0.136MetCys: 0.136 ± 0.007
0.929MetAsp: 0.929 ± 0.019
0.82MetGlu: 0.82 ± 0.017
0.448MetPhe: 0.448 ± 0.014
1.339MetGly: 1.339 ± 0.025
0.371MetHis: 0.371 ± 0.013
0.665MetIle: 0.665 ± 0.018
0.482MetLys: 0.482 ± 0.015
1.684MetLeu: 1.684 ± 0.03
0.309MetMet: 0.309 ± 0.012
0.47MetAsn: 0.47 ± 0.014
1.172MetPro: 1.172 ± 0.021
0.486MetGln: 0.486 ± 0.014
1.44MetArg: 1.44 ± 0.024
1.336MetSer: 1.336 ± 0.025
1.587MetThr: 1.587 ± 0.025
1.276MetVal: 1.276 ± 0.023
0.206MetTrp: 0.206 ± 0.009
0.354MetTyr: 0.354 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
2.306AsnAla: 2.306 ± 0.03
0.18AsnCys: 0.18 ± 0.008
1.018AsnAsp: 1.018 ± 0.023
0.93AsnGlu: 0.93 ± 0.024
0.508AsnPhe: 0.508 ± 0.017
2.022AsnGly: 2.022 ± 0.035
0.419AsnHis: 0.419 ± 0.015
0.704AsnIle: 0.704 ± 0.019
0.485AsnLys: 0.485 ± 0.015
1.714AsnLeu: 1.714 ± 0.031
0.311AsnMet: 0.311 ± 0.013
0.458AsnAsn: 0.458 ± 0.018
1.471AsnPro: 1.471 ± 0.027
0.587AsnGln: 0.587 ± 0.018
1.333AsnArg: 1.333 ± 0.024
1.016AsnSer: 1.016 ± 0.023
1.164AsnThr: 1.164 ± 0.028
1.422AsnVal: 1.422 ± 0.028
0.319AsnTrp: 0.319 ± 0.012
0.429AsnTyr: 0.429 ± 0.014
0.0AsnXaa: 0.0 ± 0.0
Pro
8.045ProAla: 8.045 ± 0.089
0.345ProCys: 0.345 ± 0.013
4.443ProAsp: 4.443 ± 0.044
4.463ProGlu: 4.463 ± 0.046
1.516ProPhe: 1.516 ± 0.025
6.354ProGly: 6.354 ± 0.066
1.484ProHis: 1.484 ± 0.029
1.421ProIle: 1.421 ± 0.026
1.376ProLys: 1.376 ± 0.031
5.283ProLeu: 5.283 ± 0.053
1.007ProMet: 1.007 ± 0.022
0.969ProAsn: 0.969 ± 0.022
3.548ProPro: 3.548 ± 0.054
1.955ProGln: 1.955 ± 0.037
3.883ProArg: 3.883 ± 0.046
3.417ProSer: 3.417 ± 0.045
3.24ProThr: 3.24 ± 0.04
5.344ProVal: 5.344 ± 0.051
0.912ProTrp: 0.912 ± 0.018
1.509ProTyr: 1.509 ± 0.025
0.0ProXaa: 0.0 ± 0.0
Gln
3.987GlnAla: 3.987 ± 0.045
0.203GlnCys: 0.203 ± 0.009
1.538GlnAsp: 1.538 ± 0.027
1.597GlnGlu: 1.597 ± 0.029
0.742GlnPhe: 0.742 ± 0.02
2.451GlnGly: 2.451 ± 0.04
0.764GlnHis: 0.764 ± 0.019
1.197GlnIle: 1.197 ± 0.025
0.699GlnLys: 0.699 ± 0.021
3.357GlnLeu: 3.357 ± 0.042
0.568GlnMet: 0.568 ± 0.015
0.533GlnAsn: 0.533 ± 0.014
1.885GlnPro: 1.885 ± 0.034
1.456GlnGln: 1.456 ± 0.039
2.625GlnArg: 2.625 ± 0.042
1.387GlnSer: 1.387 ± 0.028
1.466GlnThr: 1.466 ± 0.031
2.462GlnVal: 2.462 ± 0.032
0.495GlnTrp: 0.495 ± 0.017
0.65GlnTyr: 0.65 ± 0.017
0.0GlnXaa: 0.0 ± 0.0
Arg
9.391ArgAla: 9.391 ± 0.084
0.602ArgCys: 0.602 ± 0.016
4.36ArgAsp: 4.36 ± 0.05
4.811ArgGlu: 4.811 ± 0.046
2.282ArgPhe: 2.282 ± 0.037
5.738ArgGly: 5.738 ± 0.054
2.117ArgHis: 2.117 ± 0.033
3.277ArgIle: 3.277 ± 0.039
1.835ArgLys: 1.835 ± 0.034
8.575ArgLeu: 8.575 ± 0.082
1.736ArgMet: 1.736 ± 0.029
1.409ArgAsn: 1.409 ± 0.028
4.719ArgPro: 4.719 ± 0.055
2.473ArgGln: 2.473 ± 0.037
7.412ArgArg: 7.412 ± 0.076
4.003ArgSer: 4.003 ± 0.044
5.248ArgThr: 5.248 ± 0.051
5.689ArgVal: 5.689 ± 0.047
1.382ArgTrp: 1.382 ± 0.025
1.827ArgTyr: 1.827 ± 0.029
0.0ArgXaa: 0.0 ± 0.0
Ser
6.808SerAla: 6.808 ± 0.067
0.427SerCys: 0.427 ± 0.013
2.877SerAsp: 2.877 ± 0.035
2.662SerGlu: 2.662 ± 0.035
1.544SerPhe: 1.544 ± 0.03
5.951SerGly: 5.951 ± 0.064
1.059SerHis: 1.059 ± 0.021
1.516SerIle: 1.516 ± 0.027
1.2SerLys: 1.2 ± 0.028
4.831SerLeu: 4.831 ± 0.044
1.164SerMet: 1.164 ± 0.023
0.938SerAsn: 0.938 ± 0.021
3.289SerPro: 3.289 ± 0.047
1.397SerGln: 1.397 ± 0.026
3.707SerArg: 3.707 ± 0.046
3.047SerSer: 3.047 ± 0.046
3.163SerThr: 3.163 ± 0.042
4.3SerVal: 4.3 ± 0.045
0.894SerTrp: 0.894 ± 0.021
1.319SerTyr: 1.319 ± 0.026
0.0SerXaa: 0.0 ± 0.0
Thr
8.638ThrAla: 8.638 ± 0.062
0.453ThrCys: 0.453 ± 0.015
3.667ThrAsp: 3.667 ± 0.042
3.383ThrGlu: 3.383 ± 0.041
1.626ThrPhe: 1.626 ± 0.029
6.31ThrGly: 6.31 ± 0.057
1.241ThrHis: 1.241 ± 0.022
1.856ThrIle: 1.856 ± 0.031
1.34ThrLys: 1.34 ± 0.03
5.607ThrLeu: 5.607 ± 0.049
0.961ThrMet: 0.961 ± 0.021
1.045ThrAsn: 1.045 ± 0.023
4.24ThrPro: 4.24 ± 0.045
1.495ThrGln: 1.495 ± 0.026
3.852ThrArg: 3.852 ± 0.039
3.302ThrSer: 3.302 ± 0.048
3.931ThrThr: 3.931 ± 0.056
5.916ThrVal: 5.916 ± 0.056
0.94ThrTrp: 0.94 ± 0.023
1.404ThrTyr: 1.404 ± 0.028
0.0ThrXaa: 0.0 ± 0.0
Val
10.069ValAla: 10.069 ± 0.067
0.738ValCys: 0.738 ± 0.019
4.931ValAsp: 4.931 ± 0.05
4.726ValGlu: 4.726 ± 0.046
2.31ValPhe: 2.31 ± 0.037
6.239ValGly: 6.239 ± 0.057
1.983ValHis: 1.983 ± 0.03
3.032ValIle: 3.032 ± 0.042
1.885ValLys: 1.885 ± 0.036
8.936ValLeu: 8.936 ± 0.078
1.449ValMet: 1.449 ± 0.028
1.692ValAsn: 1.692 ± 0.028
5.059ValPro: 5.059 ± 0.048
2.26ValGln: 2.26 ± 0.031
6.972ValArg: 6.972 ± 0.056
4.322ValSer: 4.322 ± 0.044
5.496ValThr: 5.496 ± 0.054
7.61ValVal: 7.61 ± 0.067
1.156ValTrp: 1.156 ± 0.024
1.606ValTyr: 1.606 ± 0.026
0.0ValXaa: 0.0 ± 0.0
Trp
1.654TrpAla: 1.654 ± 0.031
0.167TrpCys: 0.167 ± 0.007
0.864TrpAsp: 0.864 ± 0.023
0.765TrpGlu: 0.765 ± 0.018
0.502TrpPhe: 0.502 ± 0.016
1.097TrpGly: 1.097 ± 0.024
0.401TrpHis: 0.401 ± 0.014
0.574TrpIle: 0.574 ± 0.017
0.402TrpLys: 0.402 ± 0.014
1.809TrpLeu: 1.809 ± 0.032
0.29TrpMet: 0.29 ± 0.014
0.431TrpAsn: 0.431 ± 0.013
0.815TrpPro: 0.815 ± 0.019
0.646TrpGln: 0.646 ± 0.016
1.338TrpArg: 1.338 ± 0.026
0.942TrpSer: 0.942 ± 0.021
1.048TrpThr: 1.048 ± 0.025
0.984TrpVal: 0.984 ± 0.022
0.353TrpTrp: 0.353 ± 0.013
0.366TrpTyr: 0.366 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.87TyrAla: 2.87 ± 0.039
0.19TyrCys: 0.19 ± 0.01
1.635TyrAsp: 1.635 ± 0.029
1.398TyrGlu: 1.398 ± 0.028
0.64TyrPhe: 0.64 ± 0.018
2.336TyrGly: 2.336 ± 0.035
0.393TyrHis: 0.393 ± 0.011
0.569TyrIle: 0.569 ± 0.016
0.47TyrLys: 0.47 ± 0.015
2.095TyrLeu: 2.095 ± 0.031
0.28TyrMet: 0.28 ± 0.009
0.401TyrAsn: 0.401 ± 0.013
1.078TyrPro: 1.078 ± 0.023
0.666TyrGln: 0.666 ± 0.019
1.873TyrArg: 1.873 ± 0.033
0.967TyrSer: 0.967 ± 0.022
1.179TyrThr: 1.179 ± 0.022
1.721TyrVal: 1.721 ± 0.027
0.357TyrTrp: 0.357 ± 0.012
0.464TyrTyr: 0.464 ± 0.016
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 7341 proteins (2323136 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski