Amino acid dipepetide frequency for bacterium C-53

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.104AlaAla: 7.104 ± 0.112	1.058AlaCys: 1.058 ± 0.034	4.529AlaAsp: 4.529 ± 0.06	5.604AlaGlu: 5.604 ± 0.084	3.058AlaPhe: 3.058 ± 0.055	6.039AlaGly: 6.039 ± 0.069	0.979AlaHis: 0.979 ± 0.029	4.519AlaIle: 4.519 ± 0.069	4.642AlaLys: 4.642 ± 0.066	6.689AlaLeu: 6.689 ± 0.079	2.231AlaMet: 2.231 ± 0.046	2.446AlaAsn: 2.446 ± 0.052	1.904AlaPro: 1.904 ± 0.047	2.318AlaGln: 2.318 ± 0.046	2.886AlaArg: 2.886 ± 0.05	4.046AlaSer: 4.046 ± 0.07	2.716AlaThr: 2.716 ± 0.058	6.599AlaVal: 6.599 ± 0.078	0.567AlaTrp: 0.567 ± 0.022	2.765AlaTyr: 2.765 ± 0.041	0.0AlaXaa: 0.0 ± 0.0
Cys	1.06CysAla: 1.06 ± 0.03	0.272CysCys: 0.272 ± 0.016	0.861CysAsp: 0.861 ± 0.03	0.937CysGlu: 0.937 ± 0.029	0.716CysPhe: 0.716 ± 0.027	1.468CysGly: 1.468 ± 0.044	0.314CysHis: 0.314 ± 0.014	1.211CysIle: 1.211 ± 0.034	0.779CysLys: 0.779 ± 0.027	1.297CysLeu: 1.297 ± 0.033	0.521CysMet: 0.521 ± 0.021	0.634CysAsn: 0.634 ± 0.024	0.593CysPro: 0.593 ± 0.026	0.398CysGln: 0.398 ± 0.019	0.9CysArg: 0.9 ± 0.029	0.868CysSer: 0.868 ± 0.027	0.737CysThr: 0.737 ± 0.026	1.047CysVal: 1.047 ± 0.032	0.105CysTrp: 0.105 ± 0.01	0.596CysTyr: 0.596 ± 0.024	0.0CysXaa: 0.0 ± 0.0
Asp	4.17AspAla: 4.17 ± 0.072	0.836AspCys: 0.836 ± 0.025	2.921AspAsp: 2.921 ± 0.059	4.591AspGlu: 4.591 ± 0.059	2.773AspPhe: 2.773 ± 0.048	4.294AspGly: 4.294 ± 0.074	0.849AspHis: 0.849 ± 0.03	4.656AspIle: 4.656 ± 0.074	3.319AspLys: 3.319 ± 0.062	4.46AspLeu: 4.46 ± 0.072	1.842AspMet: 1.842 ± 0.04	2.176AspAsn: 2.176 ± 0.048	1.383AspPro: 1.383 ± 0.037	1.269AspGln: 1.269 ± 0.033	2.886AspArg: 2.886 ± 0.05	3.145AspSer: 3.145 ± 0.059	3.209AspThr: 3.209 ± 0.059	3.721AspVal: 3.721 ± 0.057	0.585AspTrp: 0.585 ± 0.025	2.835AspTyr: 2.835 ± 0.054	0.0AspXaa: 0.0 ± 0.0
Glu	5.597GluAla: 5.597 ± 0.087	0.936GluCys: 0.936 ± 0.029	4.273GluAsp: 4.273 ± 0.061	7.55GluGlu: 7.55 ± 0.107	2.876GluPhe: 2.876 ± 0.057	4.368GluGly: 4.368 ± 0.067	1.274GluHis: 1.274 ± 0.034	6.216GluIle: 6.216 ± 0.087	6.876GluLys: 6.876 ± 0.102	6.766GluLeu: 6.766 ± 0.087	2.604GluMet: 2.604 ± 0.047	4.346GluAsn: 4.346 ± 0.065	2.059GluPro: 2.059 ± 0.06	2.983GluGln: 2.983 ± 0.06	4.039GluArg: 4.039 ± 0.07	3.784GluSer: 3.784 ± 0.052	3.774GluThr: 3.774 ± 0.06	4.359GluVal: 4.359 ± 0.069	0.753GluTrp: 0.753 ± 0.026	3.516GluTyr: 3.516 ± 0.062	0.0GluXaa: 0.0 ± 0.0
Phe	2.949PheAla: 2.949 ± 0.051	0.889PheCys: 0.889 ± 0.026	2.755PheAsp: 2.755 ± 0.043	2.886PheGlu: 2.886 ± 0.048	2.093PhePhe: 2.093 ± 0.045	2.961PheGly: 2.961 ± 0.054	0.955PheHis: 0.955 ± 0.031	2.981PheIle: 2.981 ± 0.055	1.977PheLys: 1.977 ± 0.043	4.324PheLeu: 4.324 ± 0.071	1.286PheMet: 1.286 ± 0.034	1.519PheAsn: 1.519 ± 0.036	1.434PhePro: 1.434 ± 0.033	1.426PheGln: 1.426 ± 0.033	2.002PheArg: 2.002 ± 0.041	3.091PheSer: 3.091 ± 0.049	2.278PheThr: 2.278 ± 0.053	2.907PheVal: 2.907 ± 0.057	0.409PheTrp: 0.409 ± 0.023	1.983PheTyr: 1.983 ± 0.043	0.0PheXaa: 0.0 ± 0.0
Gly	4.831GlyAla: 4.831 ± 0.088	1.258GlyCys: 1.258 ± 0.038	3.313GlyAsp: 3.313 ± 0.053	4.71GlyGlu: 4.71 ± 0.076	3.284GlyPhe: 3.284 ± 0.046	4.59GlyGly: 4.59 ± 0.086	1.133GlyHis: 1.133 ± 0.035	6.389GlyIle: 6.389 ± 0.078	5.324GlyLys: 5.324 ± 0.064	5.504GlyLeu: 5.504 ± 0.071	2.515GlyMet: 2.515 ± 0.044	3.288GlyAsn: 3.288 ± 0.055	1.152GlyPro: 1.152 ± 0.056	2.005GlyGln: 2.005 ± 0.043	3.411GlyArg: 3.411 ± 0.062	3.857GlySer: 3.857 ± 0.057	3.837GlyThr: 3.837 ± 0.066	4.622GlyVal: 4.622 ± 0.078	0.659GlyTrp: 0.659 ± 0.024	3.321GlyTyr: 3.321 ± 0.057	0.0GlyXaa: 0.0 ± 0.0
His	1.119HisAla: 1.119 ± 0.028	0.289HisCys: 0.289 ± 0.016	0.874HisAsp: 0.874 ± 0.03	1.178HisGlu: 1.178 ± 0.034	0.896HisPhe: 0.896 ± 0.03	1.156HisGly: 1.156 ± 0.031	0.405HisHis: 0.405 ± 0.019	1.461HisIle: 1.461 ± 0.037	0.983HisLys: 0.983 ± 0.034	1.49HisLeu: 1.49 ± 0.037	0.585HisMet: 0.585 ± 0.02	0.759HisAsn: 0.759 ± 0.026	0.831HisPro: 0.831 ± 0.031	0.527HisGln: 0.527 ± 0.023	0.82HisArg: 0.82 ± 0.027	1.0HisSer: 1.0 ± 0.035	0.965HisThr: 0.965 ± 0.03	1.091HisVal: 1.091 ± 0.031	0.163HisTrp: 0.163 ± 0.01	0.83HisTyr: 0.83 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	5.557IleAla: 5.557 ± 0.069	1.417IleCys: 1.417 ± 0.034	4.191IleAsp: 4.191 ± 0.062	5.204IleGlu: 5.204 ± 0.071	3.121IlePhe: 3.121 ± 0.063	5.076IleGly: 5.076 ± 0.083	1.57IleHis: 1.57 ± 0.034	5.134IleIle: 5.134 ± 0.096	4.162IleLys: 4.162 ± 0.077	7.458IleLeu: 7.458 ± 0.099	2.141IleMet: 2.141 ± 0.047	2.916IleAsn: 2.916 ± 0.052	3.091IlePro: 3.091 ± 0.051	2.33IleGln: 2.33 ± 0.038	4.081IleArg: 4.081 ± 0.062	4.949IleSer: 4.949 ± 0.078	4.114IleThr: 4.114 ± 0.069	5.019IleVal: 5.019 ± 0.074	0.587IleTrp: 0.587 ± 0.023	2.997IleTyr: 2.997 ± 0.047	0.0IleXaa: 0.0 ± 0.0
Lys	4.812LysAla: 4.812 ± 0.071	0.811LysCys: 0.811 ± 0.025	3.821LysAsp: 3.821 ± 0.055	6.86LysGlu: 6.86 ± 0.089	1.953LysPhe: 1.953 ± 0.04	4.268LysGly: 4.268 ± 0.06	1.022LysHis: 1.022 ± 0.03	5.08LysIle: 5.08 ± 0.074	5.988LysLys: 5.988 ± 0.085	5.291LysLeu: 5.291 ± 0.079	2.096LysMet: 2.096 ± 0.038	3.54LysAsn: 3.54 ± 0.05	1.968LysPro: 1.968 ± 0.044	2.323LysGln: 2.323 ± 0.044	3.38LysArg: 3.38 ± 0.063	3.554LysSer: 3.554 ± 0.06	3.571LysThr: 3.571 ± 0.064	3.98LysVal: 3.98 ± 0.065	0.598LysTrp: 0.598 ± 0.025	2.957LysTyr: 2.957 ± 0.052	0.0LysXaa: 0.0 ± 0.0
Leu	6.277LeuAla: 6.277 ± 0.087	1.506LeuCys: 1.506 ± 0.042	4.658LeuAsp: 4.658 ± 0.071	5.887LeuGlu: 5.887 ± 0.079	4.261LeuPhe: 4.261 ± 0.081	5.394LeuGly: 5.394 ± 0.072	1.614LeuHis: 1.614 ± 0.038	6.104LeuIle: 6.104 ± 0.085	6.321LeuLys: 6.321 ± 0.08	8.653LeuLeu: 8.653 ± 0.099	2.683LeuMet: 2.683 ± 0.049	4.009LeuAsn: 4.009 ± 0.055	3.303LeuPro: 3.303 ± 0.054	2.668LeuGln: 2.668 ± 0.05	3.993LeuArg: 3.993 ± 0.056	6.646LeuSer: 6.646 ± 0.077	5.029LeuThr: 5.029 ± 0.071	5.184LeuVal: 5.184 ± 0.079	0.775LeuTrp: 0.775 ± 0.026	3.585LeuTyr: 3.585 ± 0.055	0.0LeuXaa: 0.0 ± 0.0
Met	2.372MetAla: 2.372 ± 0.05	0.358MetCys: 0.358 ± 0.018	1.869MetAsp: 1.869 ± 0.043	2.841MetGlu: 2.841 ± 0.056	1.031MetPhe: 1.031 ± 0.031	2.037MetGly: 2.037 ± 0.04	0.459MetHis: 0.459 ± 0.019	2.357MetIle: 2.357 ± 0.048	2.681MetLys: 2.681 ± 0.046	2.668MetLeu: 2.668 ± 0.049	0.941MetMet: 0.941 ± 0.03	1.685MetAsn: 1.685 ± 0.038	1.098MetPro: 1.098 ± 0.027	1.16MetGln: 1.16 ± 0.03	1.434MetArg: 1.434 ± 0.037	1.776MetSer: 1.776 ± 0.04	1.723MetThr: 1.723 ± 0.038	1.91MetVal: 1.91 ± 0.04	0.221MetTrp: 0.221 ± 0.014	0.898MetTyr: 0.898 ± 0.029	0.0MetXaa: 0.0 ± 0.0
Asn	3.242AsnAla: 3.242 ± 0.062	0.623AsnCys: 0.623 ± 0.024	2.218AsnAsp: 2.218 ± 0.048	3.116AsnGlu: 3.116 ± 0.054	1.627AsnPhe: 1.627 ± 0.04	3.485AsnGly: 3.485 ± 0.069	0.988AsnHis: 0.988 ± 0.033	3.568AsnIle: 3.568 ± 0.06	2.403AsnLys: 2.403 ± 0.05	3.856AsnLeu: 3.856 ± 0.054	1.447AsnMet: 1.447 ± 0.034	1.795AsnAsn: 1.795 ± 0.042	1.924AsnPro: 1.924 ± 0.04	1.529AsnGln: 1.529 ± 0.042	2.375AsnArg: 2.375 ± 0.05	2.422AsnSer: 2.422 ± 0.047	2.354AsnThr: 2.354 ± 0.048	2.778AsnVal: 2.778 ± 0.045	0.386AsnTrp: 0.386 ± 0.018	1.923AsnTyr: 1.923 ± 0.043	0.0AsnXaa: 0.0 ± 0.0
Pro	2.244ProAla: 2.244 ± 0.046	0.439ProCys: 0.439 ± 0.024	2.303ProAsp: 2.303 ± 0.051	3.168ProGlu: 3.168 ± 0.066	1.583ProPhe: 1.583 ± 0.032	2.063ProGly: 2.063 ± 0.047	0.541ProHis: 0.541 ± 0.023	1.92ProIle: 1.92 ± 0.04	1.854ProLys: 1.854 ± 0.037	2.63ProLeu: 2.63 ± 0.051	0.853ProMet: 0.853 ± 0.026	1.174ProAsn: 1.174 ± 0.031	0.743ProPro: 0.743 ± 0.027	0.931ProGln: 0.931 ± 0.031	1.029ProArg: 1.029 ± 0.029	1.688ProSer: 1.688 ± 0.039	1.353ProThr: 1.353 ± 0.039	3.071ProVal: 3.071 ± 0.048	0.271ProTrp: 0.271 ± 0.018	1.476ProTyr: 1.476 ± 0.04	0.0ProXaa: 0.0 ± 0.0
Gln	2.357GlnAla: 2.357 ± 0.046	0.386GlnCys: 0.386 ± 0.019	1.547GlnAsp: 1.547 ± 0.036	2.795GlnGlu: 2.795 ± 0.057	1.218GlnPhe: 1.218 ± 0.032	1.892GlnGly: 1.892 ± 0.041	0.409GlnHis: 0.409 ± 0.018	2.699GlnIle: 2.699 ± 0.05	2.77GlnLys: 2.77 ± 0.048	2.576GlnLeu: 2.576 ± 0.052	1.198GlnMet: 1.198 ± 0.03	1.705GlnAsn: 1.705 ± 0.04	0.859GlnPro: 0.859 ± 0.027	1.083GlnGln: 1.083 ± 0.038	1.44GlnArg: 1.44 ± 0.038	1.686GlnSer: 1.686 ± 0.034	1.771GlnThr: 1.771 ± 0.043	1.747GlnVal: 1.747 ± 0.039	0.313GlnTrp: 0.313 ± 0.019	1.419GlnTyr: 1.419 ± 0.04	0.0GlnXaa: 0.0 ± 0.0
Arg	2.92ArgAla: 2.92 ± 0.047	0.673ArgCys: 0.673 ± 0.024	2.445ArgAsp: 2.445 ± 0.049	4.538ArgGlu: 4.538 ± 0.084	2.097ArgPhe: 2.097 ± 0.036	2.666ArgGly: 2.666 ± 0.045	0.82ArgHis: 0.82 ± 0.029	3.865ArgIle: 3.865 ± 0.063	4.042ArgLys: 4.042 ± 0.058	4.191ArgLeu: 4.191 ± 0.063	1.731ArgMet: 1.731 ± 0.038	2.484ArgAsn: 2.484 ± 0.052	1.329ArgPro: 1.329 ± 0.035	1.811ArgGln: 1.811 ± 0.043	2.644ArgArg: 2.644 ± 0.053	2.323ArgSer: 2.323 ± 0.046	2.339ArgThr: 2.339 ± 0.048	2.769ArgVal: 2.769 ± 0.047	0.399ArgTrp: 0.399 ± 0.016	2.054ArgTyr: 2.054 ± 0.043	0.0ArgXaa: 0.0 ± 0.0
Ser	4.307SerAla: 4.307 ± 0.075	0.835SerCys: 0.835 ± 0.027	3.402SerAsp: 3.402 ± 0.056	4.423SerGlu: 4.423 ± 0.064	2.763SerPhe: 2.763 ± 0.058	5.076SerGly: 5.076 ± 0.072	1.048SerHis: 1.048 ± 0.032	4.278SerIle: 4.278 ± 0.07	3.265SerLys: 3.265 ± 0.054	5.25SerLeu: 5.25 ± 0.074	1.853SerMet: 1.853 ± 0.039	2.191SerAsn: 2.191 ± 0.044	1.681SerPro: 1.681 ± 0.045	1.817SerGln: 1.817 ± 0.039	2.85SerArg: 2.85 ± 0.053	3.472SerSer: 3.472 ± 0.066	2.546SerThr: 2.546 ± 0.045	4.715SerVal: 4.715 ± 0.069	0.497SerTrp: 0.497 ± 0.021	2.511SerTyr: 2.511 ± 0.048	0.0SerXaa: 0.0 ± 0.0
Thr	4.26ThrAla: 4.26 ± 0.07	0.602ThrCys: 0.602 ± 0.023	3.201ThrAsp: 3.201 ± 0.059	4.086ThrGlu: 4.086 ± 0.071	2.069ThrPhe: 2.069 ± 0.042	4.545ThrGly: 4.545 ± 0.077	0.821ThrHis: 0.821 ± 0.028	3.753ThrIle: 3.753 ± 0.069	2.886ThrLys: 2.886 ± 0.048	4.519ThrLeu: 4.519 ± 0.065	1.389ThrMet: 1.389 ± 0.034	1.949ThrAsn: 1.949 ± 0.043	1.905ThrPro: 1.905 ± 0.041	1.444ThrGln: 1.444 ± 0.035	1.866ThrArg: 1.866 ± 0.039	2.796ThrSer: 2.796 ± 0.054	2.43ThrThr: 2.43 ± 0.049	4.287ThrVal: 4.287 ± 0.068	0.444ThrTrp: 0.444 ± 0.019	1.984ThrTyr: 1.984 ± 0.04	0.0ThrXaa: 0.0 ± 0.0
Val	4.227ValAla: 4.227 ± 0.074	1.263ValCys: 1.263 ± 0.033	3.624ValAsp: 3.624 ± 0.062	4.656ValGlu: 4.656 ± 0.072	3.208ValPhe: 3.208 ± 0.057	3.82ValGly: 3.82 ± 0.061	1.143ValHis: 1.143 ± 0.031	5.256ValIle: 5.256 ± 0.077	4.474ValLys: 4.474 ± 0.066	6.291ValLeu: 6.291 ± 0.087	2.105ValMet: 2.105 ± 0.043	2.937ValAsn: 2.937 ± 0.048	2.375ValPro: 2.375 ± 0.049	1.98ValGln: 1.98 ± 0.041	3.328ValArg: 3.328 ± 0.053	4.845ValSer: 4.845 ± 0.072	3.877ValThr: 3.877 ± 0.065	4.57ValVal: 4.57 ± 0.074	0.611ValTrp: 0.611 ± 0.024	2.799ValTyr: 2.799 ± 0.048	0.0ValXaa: 0.0 ± 0.0
Trp	0.499TrpAla: 0.499 ± 0.022	0.161TrpCys: 0.161 ± 0.013	0.525TrpAsp: 0.525 ± 0.023	0.642TrpGlu: 0.642 ± 0.023	0.458TrpPhe: 0.458 ± 0.022	0.658TrpGly: 0.658 ± 0.027	0.187TrpHis: 0.187 ± 0.011	0.607TrpIle: 0.607 ± 0.022	0.754TrpLys: 0.754 ± 0.031	0.806TrpLeu: 0.806 ± 0.026	0.277TrpMet: 0.277 ± 0.016	0.553TrpAsn: 0.553 ± 0.02	0.169TrpPro: 0.169 ± 0.013	0.346TrpGln: 0.346 ± 0.016	0.381TrpArg: 0.381 ± 0.019	0.476TrpSer: 0.476 ± 0.017	0.341TrpThr: 0.341 ± 0.017	0.513TrpVal: 0.513 ± 0.019	0.105TrpTrp: 0.105 ± 0.009	0.38TrpTyr: 0.38 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.796TyrAla: 2.796 ± 0.051	0.699TyrCys: 0.699 ± 0.029	2.59TyrAsp: 2.59 ± 0.044	3.276TyrGlu: 3.276 ± 0.057	2.021TyrPhe: 2.021 ± 0.036	3.148TyrGly: 3.148 ± 0.061	0.892TyrHis: 0.892 ± 0.026	3.065TyrIle: 3.065 ± 0.056	2.373TyrLys: 2.373 ± 0.045	3.833TyrLeu: 3.833 ± 0.058	1.22TyrMet: 1.22 ± 0.033	1.951TyrAsn: 1.951 ± 0.044	1.533TyrPro: 1.533 ± 0.037	1.519TyrGln: 1.519 ± 0.036	2.396TyrArg: 2.396 ± 0.054	2.358TyrSer: 2.358 ± 0.043	2.272TyrThr: 2.272 ± 0.052	2.501TyrVal: 2.501 ± 0.042	0.389TyrTrp: 0.389 ± 0.02	2.044TyrTyr: 2.044 ± 0.05	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3732 proteins (1175357 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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