Eufriesea mexicana

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; Apinae; Euglossini

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7068 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A310SKQ8|A0A310SKQ8_9HYME Chloride channel protein OS=Eufriesea mexicana OX=516756 GN=WN48_05835 PE=3 SV=1
MM1 pKa = 8.22DD2 pKa = 3.84MRR4 pKa = 11.84TVTMTRR10 pKa = 11.84GTSTMIIATLTMAMGILTMIIATLTMAMGILTMIIATLTMAMGILTMIIATLTMAMGILTMATGKK75 pKa = 7.42TTMITRR81 pKa = 11.84TSTMVIGTSTIATRR95 pKa = 11.84MSTMATGMSTITIATLTITMRR116 pKa = 11.84TLTMATEE123 pKa = 4.33MSTITIATLTITMTDD138 pKa = 3.49DD139 pKa = 4.16DD140 pKa = 5.1YY141 pKa = 12.21GNIDD145 pKa = 4.82DD146 pKa = 5.93GYY148 pKa = 11.24LDD150 pKa = 3.96VDD152 pKa = 3.59DD153 pKa = 5.18SYY155 pKa = 11.96RR156 pKa = 11.84GFDD159 pKa = 4.61DD160 pKa = 5.9GYY162 pKa = 11.41LDD164 pKa = 4.65VDD166 pKa = 4.23DD167 pKa = 6.2SYY169 pKa = 11.8EE170 pKa = 3.89AWTMATRR177 pKa = 11.84MSTIAVPTSTITMRR191 pKa = 11.84ADD193 pKa = 3.28DD194 pKa = 4.79GYY196 pKa = 11.56RR197 pKa = 11.84DD198 pKa = 3.43VDD200 pKa = 3.49NSYY203 pKa = 11.89GDD205 pKa = 4.24INDD208 pKa = 4.2CYY210 pKa = 11.23EE211 pKa = 4.51DD212 pKa = 3.98VDD214 pKa = 5.54DD215 pKa = 7.04GYY217 pKa = 11.89LDD219 pKa = 3.62VNDD222 pKa = 4.32YY223 pKa = 11.64YY224 pKa = 11.38EE225 pKa = 5.67DD226 pKa = 4.33FDD228 pKa = 5.67DD229 pKa = 5.94GYY231 pKa = 11.69RR232 pKa = 11.84DD233 pKa = 3.42VDD235 pKa = 4.41DD236 pKa = 5.92GYY238 pKa = 11.74LDD240 pKa = 4.89VDD242 pKa = 3.78DD243 pKa = 6.42CYY245 pKa = 11.58GGWTMVTGMATKK257 pKa = 10.15IIATSTITTRR267 pKa = 11.84TLAMATGMSTIATRR281 pKa = 11.84TLTMSTGAGQWLQEE295 pKa = 4.56CRR297 pKa = 11.84RR298 pKa = 11.84CLQGLDD304 pKa = 3.2GSYY307 pKa = 10.97RR308 pKa = 11.84DD309 pKa = 3.68INNSYY314 pKa = 11.1RR315 pKa = 11.84NVNDD319 pKa = 4.27FYY321 pKa = 11.66EE322 pKa = 4.7DD323 pKa = 3.32VDD325 pKa = 4.42DD326 pKa = 4.15GHH328 pKa = 7.82GNVNSVYY335 pKa = 10.4RR336 pKa = 11.84DD337 pKa = 3.41DD338 pKa = 4.26YY339 pKa = 12.01NNYY342 pKa = 10.23EE343 pKa = 4.4YY344 pKa = 11.76VNDD347 pKa = 4.58AYY349 pKa = 11.64SNDD352 pKa = 3.25NVEE355 pKa = 4.83VIGKK359 pKa = 7.51YY360 pKa = 7.76TKK362 pKa = 9.77QWNTDD367 pKa = 2.84

Molecular weight: 40.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A310SSF3|A0A310SSF3_9HYME Tubulin alpha chain (Fragment) OS=Eufriesea mexicana OX=516756 GN=WN48_08017 PE=3 SV=1
MM1 pKa = 7.34TGIFKK6 pKa = 9.74TRR8 pKa = 11.84GKK10 pKa = 9.62HH11 pKa = 4.4GKK13 pKa = 8.97RR14 pKa = 11.84ATRR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84NVKK23 pKa = 10.06EE24 pKa = 3.48IAGLNNATRR33 pKa = 11.84WKK35 pKa = 10.02RR36 pKa = 11.84KK37 pKa = 5.47QTGGQVPKK45 pKa = 10.31RR46 pKa = 11.84HH47 pKa = 5.9FFHH50 pKa = 7.31GGLAA54 pKa = 3.46

Molecular weight: 6.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7068

0

7068

3581618

50

16601

506.7

57.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.903 ± 0.031

1.993 ± 0.026

5.294 ± 0.024

6.983 ± 0.051

3.52 ± 0.019

5.528 ± 0.035

2.468 ± 0.019

5.948 ± 0.029

6.538 ± 0.044

8.946 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.207 ± 0.012

5.169 ± 0.032

5.036 ± 0.042

4.224 ± 0.028

5.708 ± 0.031

8.202 ± 0.038

6.09 ± 0.028

6.043 ± 0.028

1.102 ± 0.01

3.09 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski