Nocardia stercoris

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Nocardia

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6448 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3M2KRF9|A0A3M2KRF9_9NOCA Alpha/beta hydrolase OS=Nocardia stercoris OX=2483361 GN=EBN03_30765 PE=4 SV=1
MM1 pKa = 8.05CDD3 pKa = 2.89LAIAVDD9 pKa = 4.36EE10 pKa = 4.41VAAALISQAVEE21 pKa = 4.24SAVLACDD28 pKa = 4.03FDD30 pKa = 4.69YY31 pKa = 11.39DD32 pKa = 3.91SARR35 pKa = 11.84MHH37 pKa = 5.47VTVTTDD43 pKa = 2.85VTAPDD48 pKa = 3.8AVDD51 pKa = 3.12EE52 pKa = 5.12HH53 pKa = 8.08SFGWYY58 pKa = 9.23AVSAITDD65 pKa = 4.07FLSATVDD72 pKa = 3.54PYY74 pKa = 11.81DD75 pKa = 4.24PLVGGYY81 pKa = 9.02RR82 pKa = 11.84VAVEE86 pKa = 4.8FSRR89 pKa = 11.84SRR91 pKa = 11.84GEE93 pKa = 3.94IDD95 pKa = 4.85DD96 pKa = 4.41DD97 pKa = 4.01

Molecular weight: 10.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3M2LAV3|A0A3M2LAV3_9NOCA Peptidase OS=Nocardia stercoris OX=2483361 GN=EBN03_07025 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSARR37 pKa = 11.84RR38 pKa = 11.84GKK40 pKa = 10.41GRR42 pKa = 11.84AKK44 pKa = 9.65LTAA47 pKa = 4.21

Molecular weight: 5.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6448

0

6448

2145449

27

14709

332.7

35.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.883 ± 0.047

0.764 ± 0.012

6.18 ± 0.028

5.058 ± 0.029

2.893 ± 0.019

9.073 ± 0.032

2.252 ± 0.013

3.882 ± 0.024

1.725 ± 0.027

10.132 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.811 ± 0.016

1.956 ± 0.019

6.096 ± 0.027

2.877 ± 0.019

7.63 ± 0.04

5.124 ± 0.024

6.431 ± 0.029

8.689 ± 0.04

1.462 ± 0.014

2.082 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski