Psychrobacillus phage Spoks

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M5CBW0|A0A6M5CBW0_9CAUD Uncharacterized protein OS=Psychrobacillus phage Spoks OX=2736239 PE=4 SV=1
MM1 pKa = 7.34NKK3 pKa = 9.85IIFKK7 pKa = 10.58VDD9 pKa = 2.96AEE11 pKa = 4.33NNLLYY16 pKa = 9.36DD17 pKa = 3.59TQLEE21 pKa = 4.26VGIDD25 pKa = 3.81EE26 pKa = 4.39EE27 pKa = 4.87VEE29 pKa = 4.63GYY31 pKa = 10.49INTPLPTDD39 pKa = 3.76SKK41 pKa = 11.03GHH43 pKa = 5.71QLPFWKK49 pKa = 9.37PQWNGTEE56 pKa = 4.16WIEE59 pKa = 4.58ARR61 pKa = 11.84PQEE64 pKa = 4.46EE65 pKa = 4.58IEE67 pKa = 4.62AEE69 pKa = 4.0LNAPKK74 pKa = 10.38EE75 pKa = 4.15PTPLEE80 pKa = 4.7IISQDD85 pKa = 3.4VEE87 pKa = 4.33AVAEE91 pKa = 4.13MVAIVTEE98 pKa = 4.39DD99 pKa = 3.03SAMVAEE105 pKa = 4.77TVAIVVEE112 pKa = 4.66DD113 pKa = 3.82SLSVAEE119 pKa = 4.35TLALALMEE127 pKa = 4.19IEE129 pKa = 4.14NLKK132 pKa = 10.79SEE134 pKa = 3.95IAILKK139 pKa = 9.52GVV141 pKa = 3.37

Molecular weight: 15.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M5CEL4|A0A6M5CEL4_9CAUD Putative tail component OS=Psychrobacillus phage Spoks OX=2736239 PE=4 SV=1
MM1 pKa = 7.76KK2 pKa = 10.24IPWHH6 pKa = 5.77KK7 pKa = 9.97RR8 pKa = 11.84VYY10 pKa = 10.8ALYY13 pKa = 10.57KK14 pKa = 10.14GDD16 pKa = 3.56QFLSEE21 pKa = 3.93GTIRR25 pKa = 11.84EE26 pKa = 4.01IHH28 pKa = 6.44RR29 pKa = 11.84EE30 pKa = 3.82TGKK33 pKa = 10.74SVDD36 pKa = 4.24FLKK39 pKa = 11.09HH40 pKa = 4.6MTTPTYY46 pKa = 10.74SRR48 pKa = 11.84RR49 pKa = 11.84CGDD52 pKa = 3.18SLKK55 pKa = 10.48RR56 pKa = 11.84LRR58 pKa = 11.84LISLDD63 pKa = 3.92DD64 pKa = 3.66EE65 pKa = 4.7

Molecular weight: 7.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

11263

41

986

216.6

24.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.544 ± 0.427

0.533 ± 0.083

5.86 ± 0.244

8.017 ± 0.454

4.599 ± 0.222

5.789 ± 0.36

1.66 ± 0.206

7.733 ± 0.245

8.701 ± 0.435

8.044 ± 0.324

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.743 ± 0.206

6.037 ± 0.418

2.93 ± 0.183

3.454 ± 0.264

3.871 ± 0.344

6.011 ± 0.282

6.286 ± 0.384

6.641 ± 0.399

1.092 ± 0.112

3.454 ± 0.236
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski