Rose spring dwarf-associated virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Luteovirus

Average proteome isoelectric point is 8.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B3FHC0|B3FHC0_9LUTE Readthrough protein OS=Rose spring dwarf-associated virus OX=474454 PE=3 SV=1
MM1 pKa = 7.54LLFEE5 pKa = 5.04GLLTASARR13 pKa = 11.84VVKK16 pKa = 10.59DD17 pKa = 4.59FISYY21 pKa = 9.79IYY23 pKa = 10.61SRR25 pKa = 11.84LKK27 pKa = 10.03SVYY30 pKa = 9.55YY31 pKa = 10.01SLKK34 pKa = 8.92RR35 pKa = 11.84WLWTLQGKK43 pKa = 7.56FQPHH47 pKa = 6.75DD48 pKa = 3.45AFVAMVYY55 pKa = 10.79GYY57 pKa = 9.62MDD59 pKa = 3.98DD60 pKa = 4.75VEE62 pKa = 5.1DD63 pKa = 5.01FEE65 pKa = 5.67VPLALEE71 pKa = 4.25YY72 pKa = 10.71EE73 pKa = 4.37VTEE76 pKa = 4.47RR77 pKa = 11.84EE78 pKa = 4.17LKK80 pKa = 10.01EE81 pKa = 4.2AQDD84 pKa = 3.5EE85 pKa = 4.4LNRR88 pKa = 11.84LEE90 pKa = 4.26SKK92 pKa = 10.76LRR94 pKa = 11.84FTSFNPWVNLVKK106 pKa = 9.99KK107 pKa = 8.85TFTRR111 pKa = 11.84EE112 pKa = 3.67VAPEE116 pKa = 4.09PFWPVPSRR124 pKa = 11.84PDD126 pKa = 3.68CDD128 pKa = 4.24DD129 pKa = 3.91PCISSPLIEE138 pKa = 5.12DD139 pKa = 3.8EE140 pKa = 4.77EE141 pKa = 4.62EE142 pKa = 4.34VSGPEE147 pKa = 3.87PEE149 pKa = 5.71PIFSQQKK156 pKa = 9.65LDD158 pKa = 3.55EE159 pKa = 4.55RR160 pKa = 11.84LCMEE164 pKa = 4.56ARR166 pKa = 11.84MDD168 pKa = 3.75VLNSEE173 pKa = 4.41VKK175 pKa = 9.66FDD177 pKa = 4.01KK178 pKa = 10.74VRR180 pKa = 11.84EE181 pKa = 4.22RR182 pKa = 11.84INIVYY187 pKa = 10.2QEE189 pKa = 4.05VRR191 pKa = 11.84GARR194 pKa = 11.84PFGKK198 pKa = 9.76IFNTMHH204 pKa = 6.11QRR206 pKa = 11.84MKK208 pKa = 10.83HH209 pKa = 4.65VGKK212 pKa = 10.05CLKK215 pKa = 10.16RR216 pKa = 11.84RR217 pKa = 11.84SEE219 pKa = 3.75ASARR223 pKa = 11.84SVEE226 pKa = 4.02FEE228 pKa = 3.88KK229 pKa = 10.81RR230 pKa = 11.84VNAKK234 pKa = 9.05TDD236 pKa = 2.96IADD239 pKa = 3.67FHH241 pKa = 6.74SLCEE245 pKa = 4.11VEE247 pKa = 4.82EE248 pKa = 4.55VEE250 pKa = 4.29TGEE253 pKa = 3.99FHH255 pKa = 7.53PVKK258 pKa = 10.34QDD260 pKa = 3.09ADD262 pKa = 4.07GEE264 pKa = 4.33DD265 pKa = 4.08LPRR268 pKa = 11.84LPKK271 pKa = 9.97IEE273 pKa = 3.98VVRR276 pKa = 11.84RR277 pKa = 11.84IKK279 pKa = 10.71DD280 pKa = 3.32NCHH283 pKa = 6.05RR284 pKa = 11.84PAATWIRR291 pKa = 11.84EE292 pKa = 3.86YY293 pKa = 11.12VRR295 pKa = 11.84CKK297 pKa = 10.57NSQLSADD304 pKa = 4.17EE305 pKa = 4.43VSHH308 pKa = 5.81ATITRR313 pKa = 11.84YY314 pKa = 9.75VEE316 pKa = 3.98QFCEE320 pKa = 4.1KK321 pKa = 10.81NKK323 pKa = 9.81MDD325 pKa = 3.45MDD327 pKa = 3.85SRR329 pKa = 11.84VFLMQRR335 pKa = 11.84ALLMVPIPKK344 pKa = 9.98PIDD347 pKa = 3.01IDD349 pKa = 3.15IAMTVHH355 pKa = 6.45SPAARR360 pKa = 11.84EE361 pKa = 3.78LRR363 pKa = 11.84SIVDD367 pKa = 3.74TAASSVFF374 pKa = 3.36

Molecular weight: 43.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B3FHC2|B3FHC2_9LUTE Movement protein OS=Rose spring dwarf-associated virus OX=474454 PE=3 SV=1
MM1 pKa = 6.6STVVVRR7 pKa = 11.84QQARR11 pKa = 11.84NNSRR15 pKa = 11.84RR16 pKa = 11.84NGQAQQAQGRR26 pKa = 11.84SRR28 pKa = 11.84QPNKK32 pKa = 10.02ARR34 pKa = 11.84PVVVQVQPSRR44 pKa = 11.84NGRR47 pKa = 11.84RR48 pKa = 11.84PRR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84GGRR55 pKa = 11.84RR56 pKa = 11.84SSRR59 pKa = 11.84RR60 pKa = 11.84RR61 pKa = 11.84GSRR64 pKa = 11.84MASSRR69 pKa = 11.84SHH71 pKa = 5.36WEE73 pKa = 3.84DD74 pKa = 3.21YY75 pKa = 11.21KK76 pKa = 10.93FTINNLKK83 pKa = 10.64ASDD86 pKa = 3.55AGVVKK91 pKa = 9.97FGPSISQCSALKK103 pKa = 10.69SGIFKK108 pKa = 10.61SFHH111 pKa = 5.24EE112 pKa = 4.58FKK114 pKa = 9.91ITNLNVKK121 pKa = 9.61YY122 pKa = 8.8ITHH125 pKa = 6.99AASTTSGAFAVEE137 pKa = 4.15VDD139 pKa = 4.14TSCTQTTLKK148 pKa = 10.63SYY150 pKa = 10.44LQTVPVAKK158 pKa = 9.99CGQFSWPAGKK168 pKa = 9.7IRR170 pKa = 11.84GTGWLPTPDD179 pKa = 4.0PDD181 pKa = 4.09KK182 pKa = 11.37TPVDD186 pKa = 3.63KK187 pKa = 11.29DD188 pKa = 3.39NQFFLLYY195 pKa = 10.25AGNGPSSVAGQFVITARR212 pKa = 11.84CWFQSPRR219 pKa = 11.84EE220 pKa = 4.11

Molecular weight: 24.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

2474

32

901

309.3

34.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.346 ± 0.501

1.778 ± 0.239

5.457 ± 0.419

6.144 ± 1.071

4.568 ± 0.388

5.295 ± 0.757

2.142 ± 0.206

4.406 ± 0.529

5.659 ± 0.511

7.599 ± 0.803

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.344 ± 0.56

3.88 ± 0.412

6.669 ± 0.751

4.244 ± 0.609

7.922 ± 0.326

8.488 ± 0.876

5.053 ± 0.828

8.084 ± 0.319

1.334 ± 0.172

2.546 ± 0.387
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski