Streptococcus satellite phage Javan352

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZKA2|A0A4D5ZKA2_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan352 OX=2558653 GN=JavanS352_0008 PE=4 SV=1
MM1 pKa = 7.6EE2 pKa = 6.11KK3 pKa = 10.91NMTLDD8 pKa = 3.77NMTQSEE14 pKa = 4.5FDD16 pKa = 3.49NRR18 pKa = 11.84ITEE21 pKa = 4.12IKK23 pKa = 10.3DD24 pKa = 3.2RR25 pKa = 11.84NPNLFQFIIDD35 pKa = 3.88FLDD38 pKa = 4.06DD39 pKa = 3.54KK40 pKa = 10.4VTPEE44 pKa = 3.89EE45 pKa = 4.34VYY47 pKa = 11.05DD48 pKa = 3.84FLKK51 pKa = 10.18MEE53 pKa = 4.85RR54 pKa = 11.84SYY56 pKa = 11.18QVNYY60 pKa = 9.19IKK62 pKa = 10.55NYY64 pKa = 8.5KK65 pKa = 9.6ARR67 pKa = 11.84AA68 pKa = 3.49

Molecular weight: 8.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZQM5|A0A4D5ZQM5_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan352 OX=2558653 GN=JavanS352_0003 PE=4 SV=1
MM1 pKa = 7.99KK2 pKa = 9.31ITQHH6 pKa = 4.84TKK8 pKa = 9.3KK9 pKa = 10.42DD10 pKa = 3.23GSAVYY15 pKa = 10.05RR16 pKa = 11.84SSIYY20 pKa = 10.71LGIDD24 pKa = 2.85SVTGKK29 pKa = 10.14KK30 pKa = 10.39VKK32 pKa = 8.21TTISARR38 pKa = 11.84TKK40 pKa = 10.43KK41 pKa = 9.83EE42 pKa = 3.68LRR44 pKa = 11.84NKK46 pKa = 8.17ATQAKK51 pKa = 10.07VEE53 pKa = 4.17FEE55 pKa = 4.59KK56 pKa = 11.24NGSTRR61 pKa = 11.84KK62 pKa = 9.17QRR64 pKa = 11.84SHH66 pKa = 5.33ITTYY70 pKa = 11.06SEE72 pKa = 4.48LVDD75 pKa = 5.66LFWQTYY81 pKa = 5.79QHH83 pKa = 6.36TIKK86 pKa = 10.07TNTQIKK92 pKa = 10.32IKK94 pKa = 10.47GCLKK98 pKa = 10.72NYY100 pKa = 9.18LLPSFSTYY108 pKa = 11.08KK109 pKa = 10.24LDD111 pKa = 3.42KK112 pKa = 8.8LTPVIIQTQVNKK124 pKa = 9.76WADD127 pKa = 3.8EE128 pKa = 4.09YY129 pKa = 11.47NQDD132 pKa = 2.92GTGYY136 pKa = 10.61KK137 pKa = 9.95EE138 pKa = 4.0YY139 pKa = 11.21NHH141 pKa = 6.5LHH143 pKa = 6.37ALNKK147 pKa = 10.25RR148 pKa = 11.84ILQYY152 pKa = 10.81GVSIQALDD160 pKa = 3.67NNPARR165 pKa = 11.84DD166 pKa = 3.34IVIPRR171 pKa = 11.84KK172 pKa = 8.2ITRR175 pKa = 11.84DD176 pKa = 3.35KK177 pKa = 11.58QEE179 pKa = 3.32IKK181 pKa = 10.77YY182 pKa = 9.97FQDD185 pKa = 3.26QEE187 pKa = 4.42LKK189 pKa = 11.12NFLSYY194 pKa = 11.28LDD196 pKa = 3.81NLEE199 pKa = 3.97NTFVNFYY206 pKa = 10.04DD207 pKa = 3.65TVLYY211 pKa = 11.0KK212 pKa = 10.08MLLATGLRR220 pKa = 11.84IRR222 pKa = 11.84EE223 pKa = 4.14CLALEE228 pKa = 4.16WSDD231 pKa = 4.72IDD233 pKa = 4.27LQNGTIDD240 pKa = 3.28INKK243 pKa = 8.44TLNILNQVNTPKK255 pKa = 10.11TKK257 pKa = 9.97SSYY260 pKa = 9.92RR261 pKa = 11.84VLDD264 pKa = 3.58IDD266 pKa = 3.85HH267 pKa = 6.69KK268 pKa = 8.91TVLMLRR274 pKa = 11.84LYY276 pKa = 10.46RR277 pKa = 11.84ARR279 pKa = 11.84QAEE282 pKa = 4.01NGRR285 pKa = 11.84NIGLTYY291 pKa = 10.56EE292 pKa = 4.48KK293 pKa = 10.64VFSDD297 pKa = 4.51SFDD300 pKa = 3.64NYY302 pKa = 10.22VNTRR306 pKa = 11.84KK307 pKa = 9.81VDD309 pKa = 3.68YY310 pKa = 10.13RR311 pKa = 11.84LHH313 pKa = 6.06KK314 pKa = 9.82HH315 pKa = 6.03LKK317 pKa = 9.47SANCTDD323 pKa = 3.87LGFHH327 pKa = 7.4AFRR330 pKa = 11.84HH331 pKa = 4.54THH333 pKa = 6.71ASILLNAGLPYY344 pKa = 10.43KK345 pKa = 10.2EE346 pKa = 3.68IQTRR350 pKa = 11.84LGHH353 pKa = 6.33AKK355 pKa = 10.05ISVTMDD361 pKa = 3.68TYY363 pKa = 11.66SHH365 pKa = 7.23LSKK368 pKa = 10.56EE369 pKa = 4.22NQKK372 pKa = 10.33RR373 pKa = 11.84AVSFFEE379 pKa = 4.04TALEE383 pKa = 4.69KK384 pKa = 10.71INSSS388 pKa = 3.32

Molecular weight: 45.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

17

0

17

3160

53

388

185.9

21.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.861 ± 0.406

0.601 ± 0.096

6.266 ± 0.418

8.386 ± 0.645

5.19 ± 0.628

4.367 ± 0.537

1.677 ± 0.325

7.12 ± 0.424

10.854 ± 0.522

10.316 ± 0.372

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.057 ± 0.278

5.981 ± 0.417

2.057 ± 0.244

3.987 ± 0.349

4.652 ± 0.336

6.329 ± 0.436

6.234 ± 0.577

4.842 ± 0.424

0.633 ± 0.119

4.589 ± 0.315
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski