Sudan ebolavirus (strain Human/Uganda/Gulu/2000) (SEBOV) (Sudan Ebola virus)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Filoviridae; Ebolavirus; Sudan ebolavirus

Average proteome isoelectric point is 7.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q7T9D9|VGP_EBOSU Envelope glycoprotein OS=Sudan ebolavirus (strain Human/Uganda/Gulu/2000) OX=386033 GN=GP PE=1 SV=1
MM1 pKa = 8.14DD2 pKa = 3.7KK3 pKa = 10.44RR4 pKa = 11.84VRR6 pKa = 11.84GSWALGGQSEE16 pKa = 4.95VDD18 pKa = 3.45LDD20 pKa = 3.52YY21 pKa = 11.61HH22 pKa = 7.16KK23 pKa = 10.8ILTAGLSVQQGIVRR37 pKa = 11.84QRR39 pKa = 11.84VIPVYY44 pKa = 10.46VVSDD48 pKa = 3.66LEE50 pKa = 4.98GICQHH55 pKa = 7.04IIQAFEE61 pKa = 4.26AGVDD65 pKa = 4.22FQDD68 pKa = 3.94NADD71 pKa = 3.87SFLLLLCLHH80 pKa = 7.0HH81 pKa = 7.48AYY83 pKa = 10.43QGDD86 pKa = 3.29HH87 pKa = 6.85RR88 pKa = 11.84LFLKK92 pKa = 10.6SDD94 pKa = 3.15AVQYY98 pKa = 11.23LEE100 pKa = 3.88GHH102 pKa = 5.84GFRR105 pKa = 11.84FEE107 pKa = 4.0VRR109 pKa = 11.84EE110 pKa = 3.95KK111 pKa = 11.13EE112 pKa = 4.07NVHH115 pKa = 6.69RR116 pKa = 11.84LDD118 pKa = 3.57EE119 pKa = 4.45LLPNVTGGKK128 pKa = 8.51NLRR131 pKa = 11.84RR132 pKa = 11.84TLAAMPEE139 pKa = 4.26EE140 pKa = 4.61EE141 pKa = 4.34TTEE144 pKa = 4.02ANAGQFLSFASLFLPKK160 pKa = 10.39LVVGEE165 pKa = 4.31KK166 pKa = 10.46ACLEE170 pKa = 4.0KK171 pKa = 10.81VQRR174 pKa = 11.84QIQVHH179 pKa = 6.09AEE181 pKa = 3.78QGLIQYY187 pKa = 7.1PTSWQSVGHH196 pKa = 5.26MMVIFRR202 pKa = 11.84LMRR205 pKa = 11.84TNFLIKK211 pKa = 10.22FLLIHH216 pKa = 6.24QGMHH220 pKa = 5.09MVAGHH225 pKa = 7.08DD226 pKa = 3.88ANDD229 pKa = 3.93TVISNSVAQARR240 pKa = 11.84FSGLLIVKK248 pKa = 8.23TVLDD252 pKa = 5.01HH253 pKa = 7.46ILQKK257 pKa = 9.94TDD259 pKa = 2.99LGVRR263 pKa = 11.84LHH265 pKa = 6.5PLARR269 pKa = 11.84TAKK272 pKa = 9.17VKK274 pKa = 11.2NEE276 pKa = 3.66VSSFKK281 pKa = 10.85AALGSLAKK289 pKa = 10.12HH290 pKa = 5.95GEE292 pKa = 4.05YY293 pKa = 10.9APFARR298 pKa = 11.84LLNLSGVNNLEE309 pKa = 4.28HH310 pKa = 6.95GLYY313 pKa = 9.39PQLSAIALGVATAHH327 pKa = 6.58GSTLAGVNVGEE338 pKa = 4.64QYY340 pKa = 10.17QQLRR344 pKa = 11.84EE345 pKa = 4.06AATEE349 pKa = 4.1AEE351 pKa = 4.44KK352 pKa = 10.74QLQQYY357 pKa = 11.11AEE359 pKa = 4.08TRR361 pKa = 11.84EE362 pKa = 3.95LDD364 pKa = 3.56NLGLDD369 pKa = 3.69EE370 pKa = 4.23QEE372 pKa = 4.84KK373 pKa = 10.03KK374 pKa = 10.66ILMSFHH380 pKa = 5.79QKK382 pKa = 10.18KK383 pKa = 10.39NEE385 pKa = 3.61ISFQQTNAMVTLRR398 pKa = 11.84KK399 pKa = 9.42EE400 pKa = 3.98RR401 pKa = 11.84LAKK404 pKa = 9.23LTEE407 pKa = 5.16AITTASKK414 pKa = 10.59IKK416 pKa = 10.9VGDD419 pKa = 4.18RR420 pKa = 11.84YY421 pKa = 10.34PDD423 pKa = 4.13DD424 pKa = 4.19NDD426 pKa = 3.44IPFPGPIYY434 pKa = 10.59DD435 pKa = 3.73EE436 pKa = 4.34THH438 pKa = 7.08PNPSDD443 pKa = 4.43DD444 pKa = 4.54NPDD447 pKa = 3.58DD448 pKa = 4.85SRR450 pKa = 11.84DD451 pKa = 3.42TTIPGGVVDD460 pKa = 5.4PYY462 pKa = 11.61DD463 pKa = 4.69DD464 pKa = 4.05EE465 pKa = 5.39SNNYY469 pKa = 8.9PDD471 pKa = 4.65YY472 pKa = 10.89EE473 pKa = 5.2DD474 pKa = 3.74SAEE477 pKa = 4.11GTTGDD482 pKa = 4.46LDD484 pKa = 5.19LFNLDD489 pKa = 5.44DD490 pKa = 6.75DD491 pKa = 6.25DD492 pKa = 7.13DD493 pKa = 5.38DD494 pKa = 4.63SQPGPPDD501 pKa = 3.39RR502 pKa = 11.84GQSKK506 pKa = 9.29EE507 pKa = 3.71RR508 pKa = 11.84AARR511 pKa = 11.84THH513 pKa = 6.56GLQDD517 pKa = 3.45PTLDD521 pKa = 3.67GAKK524 pKa = 9.66KK525 pKa = 10.54VPEE528 pKa = 4.27LTPGSHH534 pKa = 6.03QPGNLHH540 pKa = 5.05ITKK543 pKa = 9.84PGSNTNQPQGNMSSTLQSMTPIQEE567 pKa = 3.87EE568 pKa = 4.83SEE570 pKa = 4.28PDD572 pKa = 3.58DD573 pKa = 4.95QKK575 pKa = 12.09DD576 pKa = 4.04DD577 pKa = 4.3DD578 pKa = 5.19DD579 pKa = 5.11EE580 pKa = 5.51SLTSLDD586 pKa = 3.68SEE588 pKa = 4.17GDD590 pKa = 3.33EE591 pKa = 4.62DD592 pKa = 4.27VEE594 pKa = 5.08SVSGEE599 pKa = 4.09NNPTVAPPAPVYY611 pKa = 10.83KK612 pKa = 9.99DD613 pKa = 3.05TGVDD617 pKa = 3.41TNQQNGPSNAVDD629 pKa = 3.92GQGSEE634 pKa = 4.97SEE636 pKa = 4.36ALPINPEE643 pKa = 3.8KK644 pKa = 10.91GSALEE649 pKa = 3.66EE650 pKa = 4.34TYY652 pKa = 11.48YY653 pKa = 11.12HH654 pKa = 6.95LLKK657 pKa = 9.66TQGPFEE663 pKa = 5.18AINYY667 pKa = 6.26YY668 pKa = 10.72HH669 pKa = 7.33LMSDD673 pKa = 3.58EE674 pKa = 5.12PIAFSTEE681 pKa = 3.68SGKK684 pKa = 10.92EE685 pKa = 3.83YY686 pKa = 10.35IFPDD690 pKa = 3.9SLEE693 pKa = 3.89EE694 pKa = 5.37AYY696 pKa = 9.88PPWLSEE702 pKa = 3.97KK703 pKa = 10.36EE704 pKa = 4.02ALEE707 pKa = 4.08KK708 pKa = 10.47EE709 pKa = 3.92NRR711 pKa = 11.84YY712 pKa = 9.47LVIDD716 pKa = 4.4GQQFLWPVMSLQDD729 pKa = 3.24KK730 pKa = 9.91FLAVLQHH737 pKa = 6.89DD738 pKa = 4.01

Molecular weight: 81.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q5XX03|VP30_EBOSU Transcriptional activator VP30 OS=Sudan ebolavirus (strain Human/Uganda/Gulu/2000) OX=386033 GN=VP30 PE=3 SV=1
MM1 pKa = 7.54AKK3 pKa = 9.15ATGRR7 pKa = 11.84YY8 pKa = 9.49NLVTPKK14 pKa = 10.4RR15 pKa = 11.84EE16 pKa = 4.0LEE18 pKa = 4.06QGVVFSDD25 pKa = 3.8LCNFLVTPTVQGWKK39 pKa = 10.15VYY41 pKa = 8.73WAGLEE46 pKa = 3.79FDD48 pKa = 4.64VNQKK52 pKa = 10.9GITLLNRR59 pKa = 11.84LKK61 pKa = 11.35VNDD64 pKa = 4.78FAPAWAMTRR73 pKa = 11.84NLFPHH78 pKa = 7.07LFKK81 pKa = 10.95NQQSEE86 pKa = 4.46VQTPIWALRR95 pKa = 11.84VILAAGILDD104 pKa = 3.39QLMDD108 pKa = 3.85HH109 pKa = 6.52SLIEE113 pKa = 4.25PLSGALNLIADD124 pKa = 4.44WLLTTSTNHH133 pKa = 5.83FNMRR137 pKa = 11.84TQRR140 pKa = 11.84VKK142 pKa = 10.97DD143 pKa = 3.69QLSMRR148 pKa = 11.84MLSLIRR154 pKa = 11.84SNIINFINKK163 pKa = 9.7LEE165 pKa = 4.11TLHH168 pKa = 5.52VVNYY172 pKa = 9.94KK173 pKa = 10.76GLLSSVEE180 pKa = 4.01IGTPSYY186 pKa = 11.0AIIITRR192 pKa = 11.84TNMGYY197 pKa = 10.06LVEE200 pKa = 4.14VQEE203 pKa = 4.43PDD205 pKa = 3.28KK206 pKa = 11.26SAMDD210 pKa = 3.41IRR212 pKa = 11.84HH213 pKa = 6.38PGPVKK218 pKa = 10.56FSLLHH223 pKa = 6.18EE224 pKa = 4.59STLKK228 pKa = 10.58PVATPKK234 pKa = 10.25PSSITSLIMEE244 pKa = 5.1FNSSLAII251 pKa = 4.01

Molecular weight: 28.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

5509

251

2210

612.1

68.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.208 ± 0.395

1.597 ± 0.286

5.137 ± 0.579

5.391 ± 0.512

4.084 ± 0.322

5.791 ± 0.474

2.814 ± 0.259

6.172 ± 0.66

5.046 ± 0.376

10.601 ± 0.587

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.634 ± 0.235

4.847 ± 0.269

5.899 ± 0.553

4.611 ± 0.379

5.319 ± 0.432

7.769 ± 0.369

7.152 ± 0.597

5.373 ± 0.325

1.38 ± 0.207

3.177 ± 0.415
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski