Burkholderiaceae bacterium PBA

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; unclassified Burkholderiaceae

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3449 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4U8V619|A0A4U8V619_9BURK Uncharacterized protein OS=Burkholderiaceae bacterium PBA OX=795666 GN=MW7_017410 PE=4 SV=1
MM1 pKa = 6.51THH3 pKa = 6.0VVTEE7 pKa = 3.72ACIRR11 pKa = 11.84CKK13 pKa = 10.3YY14 pKa = 7.98TDD16 pKa = 4.19CVDD19 pKa = 3.61VCPVDD24 pKa = 4.18CFRR27 pKa = 11.84EE28 pKa = 4.9GPNFLVIDD36 pKa = 4.26PDD38 pKa = 3.65EE39 pKa = 5.79CIDD42 pKa = 3.99CAVCVAEE49 pKa = 4.75CPVNAIYY56 pKa = 10.77AEE58 pKa = 4.07EE59 pKa = 4.69DD60 pKa = 3.77VPGDD64 pKa = 3.53QQDD67 pKa = 4.1FLDD70 pKa = 4.83LNAKK74 pKa = 9.28LASSWPSITKK84 pKa = 8.94TKK86 pKa = 10.89SPLPDD91 pKa = 4.13ADD93 pKa = 3.45DD94 pKa = 4.01WKK96 pKa = 11.19DD97 pKa = 3.41VTAKK101 pKa = 10.18RR102 pKa = 11.84DD103 pKa = 3.46QLVQQ107 pKa = 3.24

Molecular weight: 11.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4U8V684|A0A4U8V684_9BURK VOC family protein OS=Burkholderiaceae bacterium PBA OX=795666 GN=MW7_013265 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 8.86TAGGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3449

0

3449

1125142

29

3228

326.2

35.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.687 ± 0.054

0.974 ± 0.014

5.367 ± 0.027

5.318 ± 0.034

3.414 ± 0.026

8.191 ± 0.045

2.354 ± 0.023

4.734 ± 0.029

3.098 ± 0.037

10.442 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.605 ± 0.018

2.69 ± 0.027

5.276 ± 0.031

3.919 ± 0.026

7.132 ± 0.045

5.177 ± 0.026

5.464 ± 0.038

7.473 ± 0.038

1.354 ± 0.019

2.333 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski