Clostridium sp. CAG:265

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2325 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6KQ69|R6KQ69_9CLOT V-type ATP synthase beta chain OS=Clostridium sp. CAG:265 OX=1262787 GN=atpB PE=3 SV=1
MM1 pKa = 7.47YY2 pKa = 7.57STPSITTLPDD12 pKa = 3.45AGLVFLGAFITTCSMFIVFSAAVLKK37 pKa = 10.76DD38 pKa = 3.29AVSISEE44 pKa = 4.37SLVEE48 pKa = 3.81PAKK51 pKa = 10.96LIGILIGFSSDD62 pKa = 2.44ISGASSPSTVFVDD75 pKa = 3.41NDD77 pKa = 3.12IFEE80 pKa = 4.77PSPTKK85 pKa = 10.35IFFISCPSPIEE96 pKa = 3.97TLVSSNLTSTASGFIIGSVSTISAVLTVAPIFPCMM131 pKa = 3.89

Molecular weight: 13.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6KW20|R6KW20_9CLOT Pyruvate-flavodoxin oxidoreductase OS=Clostridium sp. CAG:265 OX=1262787 GN=BN573_00487 PE=3 SV=1
MM1 pKa = 7.99DD2 pKa = 6.77AIRR5 pKa = 11.84KK6 pKa = 8.48QEE8 pKa = 3.91IIKK11 pKa = 10.47EE12 pKa = 4.3FGRR15 pKa = 11.84HH16 pKa = 5.15EE17 pKa = 4.91GDD19 pKa = 2.98TGSPEE24 pKa = 3.87VQIALLTEE32 pKa = 5.3RR33 pKa = 11.84INSLTAHH40 pKa = 6.67LRR42 pKa = 11.84THH44 pKa = 6.65KK45 pKa = 10.02KK46 pKa = 9.42DD47 pKa = 3.03HH48 pKa = 6.28HH49 pKa = 6.11SRR51 pKa = 11.84RR52 pKa = 11.84GLLMMVGQRR61 pKa = 11.84RR62 pKa = 11.84GFLKK66 pKa = 10.78YY67 pKa = 10.07LAAQDD72 pKa = 3.38IEE74 pKa = 4.63RR75 pKa = 11.84YY76 pKa = 9.11RR77 pKa = 11.84AIISKK82 pKa = 10.5LGLRR86 pKa = 11.84RR87 pKa = 3.53

Molecular weight: 10.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2325

0

2325

726272

29

3421

312.4

35.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.737 ± 0.055

1.183 ± 0.022

5.563 ± 0.035

7.89 ± 0.053

4.188 ± 0.039

6.381 ± 0.05

1.297 ± 0.018

10.075 ± 0.064

8.855 ± 0.051

8.897 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.624 ± 0.027

6.492 ± 0.052

2.622 ± 0.026

2.152 ± 0.024

3.385 ± 0.03

6.287 ± 0.039

4.848 ± 0.038

6.664 ± 0.046

0.612 ± 0.013

4.249 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski