Tomato infectious chlorosis virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Closteroviridae; Crinivirus

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C0K277|C0K277_9CLOS p4 OS=Tomato infectious chlorosis virus OX=52135 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.89DD2 pKa = 4.37FEE4 pKa = 4.7ILIKK8 pKa = 10.43EE9 pKa = 3.98KK10 pKa = 10.71SPEE13 pKa = 3.78IFSVYY18 pKa = 9.94VNYY21 pKa = 10.29IINSYY26 pKa = 10.84YY27 pKa = 10.76NGLGPTSDD35 pKa = 5.34LLDD38 pKa = 4.97LINKK42 pKa = 8.83NFLTFSSRR50 pKa = 11.84EE51 pKa = 4.04CEE53 pKa = 3.46IDD55 pKa = 3.43SCIEE59 pKa = 4.1DD60 pKa = 4.11FDD62 pKa = 3.48IKK64 pKa = 11.25YY65 pKa = 10.45YY66 pKa = 10.15FRR68 pKa = 11.84CLEE71 pKa = 3.78YY72 pKa = 10.75CKK74 pKa = 10.37NGKK77 pKa = 9.49LIWW80 pKa = 4.15

Molecular weight: 9.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C0K284|C0K284_9CLOS p26 OS=Tomato infectious chlorosis virus OX=52135 GN=ORF8 PE=4 SV=1
MM1 pKa = 7.39AVVFYY6 pKa = 8.98LLCLFIIIILFTCRR20 pKa = 11.84ISIKK24 pKa = 10.28NIHH27 pKa = 5.71SVQHH31 pKa = 6.23RR32 pKa = 11.84FF33 pKa = 3.15

Molecular weight: 3.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

6899

33

2394

627.2

71.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.914 ± 0.362

1.87 ± 0.126

6.595 ± 0.186

5.523 ± 0.139

5.001 ± 0.154

4.914 ± 0.162

2.189 ± 0.225

6.247 ± 0.423

8.117 ± 0.231

9.552 ± 0.304

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.073 ± 0.259

6.972 ± 0.316

3.015 ± 0.204

2.406 ± 0.206

4.667 ± 0.216

8.219 ± 0.581

5.189 ± 0.136

7.276 ± 0.285

0.754 ± 0.082

4.508 ± 0.245
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski