Bacillus sp. AFS006103

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4840 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A8IEV9|A0A2A8IEV9_9BACI Uncharacterized protein OS=Bacillus sp. AFS006103 OX=2033483 GN=CN481_15035 PE=4 SV=1
MM1 pKa = 7.5SLEE4 pKa = 3.92WFDD7 pKa = 4.01RR8 pKa = 11.84VCGEE12 pKa = 4.07LQEE15 pKa = 4.49HH16 pKa = 6.69LEE18 pKa = 4.58SICEE22 pKa = 4.09EE23 pKa = 4.21YY24 pKa = 11.03DD25 pKa = 3.13QVGQMTVEE33 pKa = 4.33RR34 pKa = 11.84AAKK37 pKa = 9.24HH38 pKa = 5.09PRR40 pKa = 11.84IEE42 pKa = 4.68FYY44 pKa = 11.41VDD46 pKa = 2.79TADD49 pKa = 3.6VDD51 pKa = 4.09TEE53 pKa = 4.13EE54 pKa = 5.17LEE56 pKa = 4.19RR57 pKa = 11.84DD58 pKa = 3.96YY59 pKa = 11.3FCSLFFDD66 pKa = 4.14PQNEE70 pKa = 3.98EE71 pKa = 4.38FYY73 pKa = 10.64IDD75 pKa = 3.79TFDD78 pKa = 3.5VDD80 pKa = 3.7SGHH83 pKa = 5.15TAKK86 pKa = 10.89VILSDD91 pKa = 3.3IEE93 pKa = 5.03DD94 pKa = 4.03IIEE97 pKa = 4.01EE98 pKa = 4.26VHH100 pKa = 7.07ASLHH104 pKa = 7.05DD105 pKa = 3.91YY106 pKa = 10.93MNGDD110 pKa = 3.64DD111 pKa = 5.48HH112 pKa = 6.4YY113 pKa = 9.46TDD115 pKa = 4.67DD116 pKa = 4.74GVYY119 pKa = 10.52LQTTEE124 pKa = 4.76VSDD127 pKa = 4.83EE128 pKa = 4.12DD129 pKa = 5.49DD130 pKa = 3.44EE131 pKa = 5.59DD132 pKa = 5.2NEE134 pKa = 4.33VEE136 pKa = 4.37TVYY139 pKa = 11.13VEE141 pKa = 4.55DD142 pKa = 6.0DD143 pKa = 3.5EE144 pKa = 6.45DD145 pKa = 3.85EE146 pKa = 4.62EE147 pKa = 5.04YY148 pKa = 11.52YY149 pKa = 10.68EE150 pKa = 4.27AAQVNSDD157 pKa = 4.91DD158 pKa = 3.62IFEE161 pKa = 5.14EE162 pKa = 4.61IDD164 pKa = 3.7VDD166 pKa = 3.35WSTPEE171 pKa = 3.51VTAFKK176 pKa = 10.83HH177 pKa = 5.1EE178 pKa = 4.6DD179 pKa = 3.76EE180 pKa = 5.29VEE182 pKa = 3.93VTYY185 pKa = 10.99QFGLVQATGDD195 pKa = 3.65GVLKK199 pKa = 10.42RR200 pKa = 11.84INRR203 pKa = 11.84IWTTDD208 pKa = 3.36DD209 pKa = 3.95EE210 pKa = 4.93LIKK213 pKa = 11.03DD214 pKa = 3.59EE215 pKa = 4.46SHH217 pKa = 7.38FIFSKK222 pKa = 11.09EE223 pKa = 3.67EE224 pKa = 3.68ATTIIAMIASHH235 pKa = 6.76MDD237 pKa = 3.18QLSEE241 pKa = 4.65FEE243 pKa = 4.27FF244 pKa = 5.59

Molecular weight: 28.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A8IMB0|A0A2A8IMB0_9BACI Phosphate-specific transport system accessory protein PhoU OS=Bacillus sp. AFS006103 OX=2033483 GN=phoU PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.38RR3 pKa = 11.84TYY5 pKa = 10.17QPNSRR10 pKa = 11.84KK11 pKa = 9.39HH12 pKa = 5.91SKK14 pKa = 9.08VHH16 pKa = 5.89GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSANGRR28 pKa = 11.84KK29 pKa = 8.77VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.43GRR39 pKa = 11.84KK40 pKa = 8.78VLSAA44 pKa = 4.05

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4840

0

4840

1451607

27

2706

299.9

33.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.8 ± 0.041

0.781 ± 0.012

4.89 ± 0.026

7.183 ± 0.039

4.683 ± 0.027

6.94 ± 0.037

2.018 ± 0.016

8.073 ± 0.038

7.227 ± 0.038

9.876 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.714 ± 0.018

4.686 ± 0.026

3.638 ± 0.022

3.65 ± 0.022

3.923 ± 0.025

6.05 ± 0.025

5.414 ± 0.031

6.877 ± 0.028

1.044 ± 0.013

3.533 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski