Ramularia collo-cygni

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Dothideomycetidae; Mycosphaerellales; Mycosphaerellaceae; Ramularia

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11621 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D3VBC9|A0A2D3VBC9_9PEZI Deoxyuridine 5'-triphosphate nucleotidohydrolase OS=Ramularia collo-cygni OX=112498 GN=RCC_03609 PE=3 SV=1
MM1 pKa = 7.86ADD3 pKa = 4.66FKK5 pKa = 10.86PTLDD9 pKa = 4.33DD10 pKa = 4.28LLEE13 pKa = 4.94HH14 pKa = 6.76GLGSGTPPPDD24 pKa = 4.62LPDD27 pKa = 4.47CPVCMEE33 pKa = 4.75PFRR36 pKa = 11.84DD37 pKa = 3.5LAAVITPCLHH47 pKa = 5.86WFCRR51 pKa = 11.84EE52 pKa = 3.9CLTALVKK59 pKa = 10.63SGHH62 pKa = 6.13KK63 pKa = 9.53NAHH66 pKa = 6.06KK67 pKa = 10.52CPSCRR72 pKa = 11.84YY73 pKa = 8.67EE74 pKa = 3.81MCEE77 pKa = 3.94DD78 pKa = 3.83PNKK81 pKa = 10.42KK82 pKa = 10.15VEE84 pKa = 4.27DD85 pKa = 3.49AHH87 pKa = 6.86NAEE90 pKa = 4.23EE91 pKa = 4.59DD92 pKa = 3.72APEE95 pKa = 3.95QEE97 pKa = 4.6AGVGLATIYY106 pKa = 10.97EE107 pKa = 4.56NIDD110 pKa = 3.24VAIGPDD116 pKa = 3.82GAALDD121 pKa = 4.76DD122 pKa = 5.65SDD124 pKa = 6.16DD125 pKa = 3.57EE126 pKa = 6.09DD127 pKa = 4.22YY128 pKa = 11.54ASSSDD133 pKa = 4.86DD134 pKa = 4.53GDD136 pKa = 4.12DD137 pKa = 4.06SDD139 pKa = 5.92MNMTDD144 pKa = 3.68IDD146 pKa = 5.15DD147 pKa = 4.92DD148 pKa = 5.22SEE150 pKa = 6.24DD151 pKa = 4.17DD152 pKa = 4.65GPNEE156 pKa = 3.99EE157 pKa = 5.59DD158 pKa = 5.15KK159 pKa = 10.82MDD161 pKa = 4.83SEE163 pKa = 5.73NEE165 pKa = 3.91DD166 pKa = 4.71DD167 pKa = 4.66PDD169 pKa = 4.92GDD171 pKa = 3.87YY172 pKa = 11.78EE173 pKa = 4.18EE174 pKa = 4.97

Molecular weight: 19.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D3V1Z6|A0A2D3V1Z6_9PEZI Probable homoserine O-acetyltransferase OS=Ramularia collo-cygni OX=112498 GN=RCC_10060 PE=3 SV=1
MM1 pKa = 7.88PSHH4 pKa = 6.91KK5 pKa = 10.39SFRR8 pKa = 11.84TKK10 pKa = 10.45QKK12 pKa = 9.84LAKK15 pKa = 9.55AQKK18 pKa = 8.59QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TNNTIRR36 pKa = 11.84YY37 pKa = 5.73NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.15WRR45 pKa = 11.84KK46 pKa = 7.38TRR48 pKa = 11.84IGII51 pKa = 4.0

Molecular weight: 6.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11501

120

11621

5390580

15

7005

463.9

51.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.989 ± 0.021

1.229 ± 0.009

5.778 ± 0.017

6.309 ± 0.022

3.573 ± 0.014

7.019 ± 0.022

2.425 ± 0.011

4.694 ± 0.016

4.882 ± 0.023

8.602 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.288 ± 0.009

3.621 ± 0.013

5.882 ± 0.02

4.157 ± 0.018

6.107 ± 0.02

8.182 ± 0.025

5.972 ± 0.017

6.057 ± 0.016

1.415 ± 0.008

2.652 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski