Salmo trutta (Brown trout)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Protacanthopterygii; Salmoniformes; Salmonidae;

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 114752 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A673WCC1|A0A673WCC1_SALTR Uncharacterized protein OS=Salmo trutta OX=8032 PE=4 SV=1
MM1 pKa = 7.69KK2 pKa = 10.17IIILLIIMVVSMLILLVIIIMIAVVMVVSMLVMIIMIEE40 pKa = 4.08VVMVVSMLMLIMIAVVMEE58 pKa = 3.94VSADD62 pKa = 3.38VDD64 pKa = 4.36DD65 pKa = 5.1NDD67 pKa = 4.89SGGNGSKK74 pKa = 10.87YY75 pKa = 10.9ADD77 pKa = 4.19DD78 pKa = 5.75DD79 pKa = 4.79YY80 pKa = 11.85NDD82 pKa = 3.59SGGNGSKK89 pKa = 10.84YY90 pKa = 10.75VDD92 pKa = 5.3DD93 pKa = 6.08DD94 pKa = 5.0DD95 pKa = 6.6DD96 pKa = 5.5YY97 pKa = 12.06NDD99 pKa = 3.64SGGNGSKK106 pKa = 10.79YY107 pKa = 10.94ADD109 pKa = 3.79VDD111 pKa = 3.75DD112 pKa = 6.31DD113 pKa = 4.89GYY115 pKa = 11.66NDD117 pKa = 3.58SGGNGSKK124 pKa = 10.79YY125 pKa = 10.95ADD127 pKa = 3.37VDD129 pKa = 3.49DD130 pKa = 5.6DD131 pKa = 5.13YY132 pKa = 12.08NVSKK136 pKa = 11.04YY137 pKa = 11.24ADD139 pKa = 3.73VDD141 pKa = 3.81DD142 pKa = 6.45DD143 pKa = 5.25DD144 pKa = 6.26YY145 pKa = 12.03NDD147 pKa = 3.58SGGNGSKK154 pKa = 10.79YY155 pKa = 10.97ADD157 pKa = 4.02VDD159 pKa = 3.83DD160 pKa = 6.45DD161 pKa = 5.25DD162 pKa = 6.26YY163 pKa = 12.03NDD165 pKa = 3.58SGGNGSKK172 pKa = 10.79YY173 pKa = 10.95ADD175 pKa = 3.54VDD177 pKa = 3.82DD178 pKa = 5.93DD179 pKa = 4.88YY180 pKa = 11.94NDD182 pKa = 3.5SGGNGSKK189 pKa = 10.86YY190 pKa = 10.82VDD192 pKa = 4.18DD193 pKa = 6.01DD194 pKa = 4.79YY195 pKa = 11.99NDD197 pKa = 3.51SGGNGSKK204 pKa = 10.79YY205 pKa = 10.95ADD207 pKa = 3.54VDD209 pKa = 3.82DD210 pKa = 5.93DD211 pKa = 4.88YY212 pKa = 11.94NDD214 pKa = 3.5SGGNGSKK221 pKa = 10.79YY222 pKa = 10.97ADD224 pKa = 3.05VDD226 pKa = 4.14DD227 pKa = 5.19YY228 pKa = 11.92NDD230 pKa = 3.47SGGNGSKK237 pKa = 10.79YY238 pKa = 10.9ADD240 pKa = 3.29VDD242 pKa = 4.22DD243 pKa = 5.19YY244 pKa = 11.86SDD246 pKa = 3.57SGGNGSKK253 pKa = 10.79YY254 pKa = 10.95ADD256 pKa = 3.54VDD258 pKa = 3.82DD259 pKa = 5.93DD260 pKa = 4.88YY261 pKa = 11.94NDD263 pKa = 3.5SGGNGSKK270 pKa = 10.84YY271 pKa = 10.77VDD273 pKa = 5.43DD274 pKa = 6.82DD275 pKa = 4.95DD276 pKa = 7.34DD277 pKa = 5.69DD278 pKa = 5.8YY279 pKa = 12.09NDD281 pKa = 3.64SGGNGSKK288 pKa = 10.87YY289 pKa = 10.9ADD291 pKa = 4.19DD292 pKa = 5.75DD293 pKa = 4.79YY294 pKa = 11.85NDD296 pKa = 3.59SGGNGSKK303 pKa = 10.48YY304 pKa = 10.76AAVDD308 pKa = 3.78YY309 pKa = 11.05NDD311 pKa = 3.61SGGNGSKK318 pKa = 10.79YY319 pKa = 10.9ADD321 pKa = 3.29VDD323 pKa = 4.22DD324 pKa = 5.19YY325 pKa = 11.86SDD327 pKa = 3.57SGGNGSKK334 pKa = 10.79YY335 pKa = 10.95ADD337 pKa = 3.54VDD339 pKa = 3.82DD340 pKa = 5.93DD341 pKa = 4.88YY342 pKa = 11.94NDD344 pKa = 3.5SGGNGSKK351 pKa = 10.53YY352 pKa = 10.61AADD355 pKa = 3.3VDD357 pKa = 4.74DD358 pKa = 5.37YY359 pKa = 11.77NDD361 pKa = 3.42SSGNGSKK368 pKa = 10.58YY369 pKa = 10.64AADD372 pKa = 3.3VDD374 pKa = 4.69DD375 pKa = 5.21YY376 pKa = 11.73NDD378 pKa = 3.47SGGNGSKK385 pKa = 10.53YY386 pKa = 10.61AADD389 pKa = 3.3VDD391 pKa = 4.69DD392 pKa = 5.21YY393 pKa = 11.73NDD395 pKa = 3.47SGGNGSKK402 pKa = 10.53YY403 pKa = 10.61AADD406 pKa = 3.3VDD408 pKa = 4.69DD409 pKa = 5.21YY410 pKa = 11.73NDD412 pKa = 3.47SGGNGSKK419 pKa = 10.78YY420 pKa = 10.8ADD422 pKa = 3.53GDD424 pKa = 3.83GSQTVMAHH432 pKa = 6.25GCHH435 pKa = 6.12LTDD438 pKa = 4.62EE439 pKa = 4.32EE440 pKa = 4.59LKK442 pKa = 10.94LFRR445 pKa = 11.84EE446 pKa = 4.42KK447 pKa = 10.72GASVAHH453 pKa = 6.45CPNSNISLCSGMLDD467 pKa = 3.1ARR469 pKa = 11.84RR470 pKa = 11.84VLNHH474 pKa = 6.11KK475 pKa = 10.12VKK477 pKa = 10.81LGLGTDD483 pKa = 3.48VAGGYY488 pKa = 9.17SPSMLDD494 pKa = 3.18AVRR497 pKa = 11.84RR498 pKa = 11.84TLDD501 pKa = 3.04TSKK504 pKa = 11.19ALTIQDD510 pKa = 3.78PQYY513 pKa = 9.75QTLTFEE519 pKa = 4.37EE520 pKa = 4.89VFRR523 pKa = 11.84LATLGGSQALSLDD536 pKa = 3.35EE537 pKa = 4.16HH538 pKa = 6.42TGNFQVGKK546 pKa = 10.13DD547 pKa = 3.55FDD549 pKa = 4.05ALRR552 pKa = 11.84VNAAMEE558 pKa = 4.55GGPIDD563 pKa = 5.76LINHH567 pKa = 5.97GEE569 pKa = 4.23GPKK572 pKa = 10.38VLLEE576 pKa = 4.17KK577 pKa = 10.48FLNLGDD583 pKa = 4.19DD584 pKa = 3.68RR585 pKa = 11.84NIAEE589 pKa = 4.53VYY591 pKa = 9.15VAGKK595 pKa = 9.83RR596 pKa = 11.84VVPFVEE602 pKa = 4.69RR603 pKa = 11.84PP604 pKa = 3.17

Molecular weight: 63.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A674AW04|A0A674AW04_SALTR Leiomodin-2-like OS=Salmo trutta OX=8032 GN=LOC115198757 PE=4 SV=1
KKK2 pKa = 10.25VLCGPARR9 pKa = 11.84SSGSRR14 pKa = 11.84RR15 pKa = 11.84GPLALGAVLWLSARR29 pKa = 11.84PVVLRR34 pKa = 11.84PVVLRR39 pKa = 11.84PVVLRR44 pKa = 11.84PVVLRR49 pKa = 11.84PVVLRR54 pKa = 11.84PVVLRR59 pKa = 11.84PVVLRR64 pKa = 11.84PVVLRR69 pKa = 11.84PVVLRR74 pKa = 11.84PVVLRR79 pKa = 11.84PVVLRR84 pKa = 11.84PVVLRR89 pKa = 11.84PVVLRR94 pKa = 11.84PVVLRR99 pKa = 11.84PVVLRR104 pKa = 11.84PVVLRR109 pKa = 11.84PVVLRR114 pKa = 11.84PVVLRR119 pKa = 11.84PVVLRR124 pKa = 11.84PVVLRR129 pKa = 11.84PVVLRR134 pKa = 11.84PVVLM

Molecular weight: 15.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43230

71522

114752

79092270

18

8935

689.2

77.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.245 ± 0.006

2.327 ± 0.007

5.201 ± 0.004

6.787 ± 0.01

3.637 ± 0.005

6.531 ± 0.008

2.747 ± 0.004

4.465 ± 0.005

5.525 ± 0.008

9.645 ± 0.009

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.47 ± 0.003

3.872 ± 0.004

5.643 ± 0.009

4.703 ± 0.006

5.558 ± 0.006

8.306 ± 0.008

5.817 ± 0.008

6.474 ± 0.005

1.167 ± 0.002

2.875 ± 0.004
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski