Tamus red mosaic virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Potexvirus

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|G3LHV5|G3LHV5_9VIRU Tgb1 OS=Tamus red mosaic virus OX=1081702 PE=4 SV=1
MM1 pKa = 7.3SRR3 pKa = 11.84LRR5 pKa = 11.84GALEE9 pKa = 3.9QFTDD13 pKa = 3.93PAQKK17 pKa = 10.15IVIQNEE23 pKa = 4.32QYY25 pKa = 9.54QHH27 pKa = 5.35LRR29 pKa = 11.84KK30 pKa = 7.83TLSHH34 pKa = 6.58TATTNPYY41 pKa = 10.4AIAEE45 pKa = 4.28SAANVLEE52 pKa = 4.19DD53 pKa = 5.01LGINSNPFATTAHH66 pKa = 5.31THH68 pKa = 5.42SAAKK72 pKa = 10.35AIEE75 pKa = 3.91NDD77 pKa = 3.82LYY79 pKa = 11.25FSASSLLPKK88 pKa = 10.03EE89 pKa = 4.37NPVSFLFMKK98 pKa = 9.83PAKK101 pKa = 10.18LRR103 pKa = 11.84FFHH106 pKa = 7.34RR107 pKa = 11.84GPQHH111 pKa = 6.45NDD113 pKa = 2.24HH114 pKa = 7.32FINKK118 pKa = 8.22VVEE121 pKa = 4.44PKK123 pKa = 10.6DD124 pKa = 3.51VARR127 pKa = 11.84YY128 pKa = 10.1DD129 pKa = 3.71PDD131 pKa = 3.84TLVSSLPTLSTSHH144 pKa = 7.23AFMGDD149 pKa = 3.22SLHH152 pKa = 6.5FLPFSFLVRR161 pKa = 11.84LFSDD165 pKa = 3.71NVQLKK170 pKa = 8.08TLVATMVLPAEE181 pKa = 4.46AKK183 pKa = 10.26FNLASIYY190 pKa = 10.18PNIYY194 pKa = 7.66TLQYY198 pKa = 10.01HH199 pKa = 5.85RR200 pKa = 11.84CKK202 pKa = 10.18KK203 pKa = 10.63GKK205 pKa = 8.13VTHH208 pKa = 6.66FSYY211 pKa = 11.18APGGHH216 pKa = 6.66KK217 pKa = 10.13GAEE220 pKa = 4.14YY221 pKa = 10.53VHH223 pKa = 6.38EE224 pKa = 4.7LKK226 pKa = 10.74QLDD229 pKa = 3.83WLSVGRR235 pKa = 11.84LTDD238 pKa = 3.58GVTTITAQKK247 pKa = 10.26IEE249 pKa = 4.36TKK251 pKa = 10.32AANHH255 pKa = 6.15LFIFQRR261 pKa = 11.84GSLMTPCLRR270 pKa = 11.84NFSTEE275 pKa = 4.08TKK277 pKa = 10.21FVTLPLIFLPEE288 pKa = 4.11KK289 pKa = 10.49FNARR293 pKa = 11.84QPIRR297 pKa = 11.84KK298 pKa = 6.84TLVMQMFLYY307 pKa = 10.66VKK309 pKa = 10.02GVKK312 pKa = 9.25QVSEE316 pKa = 3.94RR317 pKa = 11.84DD318 pKa = 2.79IWAKK322 pKa = 8.77WRR324 pKa = 11.84QLIKK328 pKa = 10.22TEE330 pKa = 4.12DD331 pKa = 3.73LQDD334 pKa = 4.28FSAIEE339 pKa = 4.02LLHH342 pKa = 5.39LTNYY346 pKa = 9.75FVFVGKK352 pKa = 10.23QSSINCFDD360 pKa = 4.39DD361 pKa = 3.52VLKK364 pKa = 11.2GGFLTRR370 pKa = 11.84WFKK373 pKa = 10.54PIKK376 pKa = 9.87NWFEE380 pKa = 3.82MVRR383 pKa = 11.84QKK385 pKa = 10.84IFGEE389 pKa = 4.27AEE391 pKa = 3.9FVRR394 pKa = 11.84LVRR397 pKa = 11.84ALEE400 pKa = 3.97WTEE403 pKa = 3.68VDD405 pKa = 5.29LKK407 pKa = 11.31LDD409 pKa = 3.48TKK411 pKa = 11.24NYY413 pKa = 9.53DD414 pKa = 3.35FKK416 pKa = 11.13RR417 pKa = 11.84RR418 pKa = 11.84EE419 pKa = 4.12GNGFLSLSKK428 pKa = 10.66VAAGPDD434 pKa = 3.31DD435 pKa = 4.58SEE437 pKa = 5.6DD438 pKa = 3.74VFHH441 pKa = 7.3LPQEE445 pKa = 4.26EE446 pKa = 4.85SLFSQMEE453 pKa = 4.03WLGEE457 pKa = 3.6FWNFIKK463 pKa = 10.96GEE465 pKa = 4.06NGKK468 pKa = 9.61KK469 pKa = 9.63VAEE472 pKa = 4.28PCPNEE477 pKa = 4.18PVCEE481 pKa = 4.16TNVDD485 pKa = 3.71EE486 pKa = 5.31DD487 pKa = 3.85QSAHH491 pKa = 4.74EE492 pKa = 4.29WLLKK496 pKa = 10.54LEE498 pKa = 4.07QTRR501 pKa = 11.84VVEE504 pKa = 4.38EE505 pKa = 4.47TPLEE509 pKa = 4.14QPTEE513 pKa = 3.99VSTTLDD519 pKa = 3.03ATLTMLPDD527 pKa = 3.74NSQMSEE533 pKa = 4.03DD534 pKa = 3.71EE535 pKa = 4.2KK536 pKa = 11.47VVAEE540 pKa = 4.18GAITEE545 pKa = 4.35SEE547 pKa = 4.06QAPEE551 pKa = 3.96EE552 pKa = 4.14KK553 pKa = 10.51AEE555 pKa = 4.16EE556 pKa = 4.37LEE558 pKa = 4.39QVDD561 pKa = 4.35SSPLQAEE568 pKa = 4.48VAQIRR573 pKa = 11.84FGTAPTKK580 pKa = 11.05AEE582 pKa = 3.81VAALIRR588 pKa = 11.84TAMKK592 pKa = 9.97QKK594 pKa = 10.47QISLEE599 pKa = 4.01PLEE602 pKa = 4.45ATAGCEE608 pKa = 3.82QEE610 pKa = 4.15MMEE613 pKa = 4.74GSSSAAVLEE622 pKa = 4.88DD623 pKa = 4.1PPATTSDD630 pKa = 3.62DD631 pKa = 3.73TAEE634 pKa = 4.8DD635 pKa = 4.15DD636 pKa = 4.71SGLTSDD642 pKa = 3.69EE643 pKa = 4.65RR644 pKa = 11.84EE645 pKa = 3.99MKK647 pKa = 10.45KK648 pKa = 10.39FADD651 pKa = 3.83EE652 pKa = 4.02ATQQVIDD659 pKa = 3.65MSKK662 pKa = 11.27GEE664 pKa = 4.13FEE666 pKa = 4.34KK667 pKa = 11.08KK668 pKa = 9.59PWAKK672 pKa = 9.59WMSILEE678 pKa = 4.05NAGFKK683 pKa = 9.79GTTCLLDD690 pKa = 3.72ANNYY694 pKa = 9.45LIQPILGVSKK704 pKa = 9.24LTARR708 pKa = 11.84AWPRR712 pKa = 11.84EE713 pKa = 4.07LTPEE717 pKa = 3.53VDD719 pKa = 3.84LFDD722 pKa = 4.04RR723 pKa = 11.84LKK725 pKa = 10.72AIHH728 pKa = 6.58RR729 pKa = 11.84HH730 pKa = 4.66PCAYY734 pKa = 9.46TISNLRR740 pKa = 11.84ARR742 pKa = 11.84AYY744 pKa = 10.6ASDD747 pKa = 3.78VKK749 pKa = 10.96NDD751 pKa = 3.34RR752 pKa = 11.84TGSFTRR758 pKa = 11.84SQDD761 pKa = 3.9LAWKK765 pKa = 9.51EE766 pKa = 4.14AFTMICEE773 pKa = 4.41SPRR776 pKa = 11.84QRR778 pKa = 11.84EE779 pKa = 3.85VALTVIHH786 pKa = 6.45GAGGSGKK793 pKa = 10.31SFALQKK799 pKa = 10.79YY800 pKa = 10.26LEE802 pKa = 4.99DD803 pKa = 4.58CPDD806 pKa = 3.75AEE808 pKa = 4.59VKK810 pKa = 10.29IVLPTNGLRR819 pKa = 11.84MDD821 pKa = 4.57WLAKK825 pKa = 10.16LPKK828 pKa = 10.04FPLTSVQTFEE838 pKa = 4.84KK839 pKa = 10.77ALXSQTAKK847 pKa = 10.54VVIFDD852 pKa = 5.68DD853 pKa = 4.12YY854 pKa = 11.44TKK856 pKa = 10.98LPNGYY861 pKa = 8.72IDD863 pKa = 5.19AYY865 pKa = 10.75LSLRR869 pKa = 11.84TTVVWAILTGDD880 pKa = 3.39SRR882 pKa = 11.84QSSFHH887 pKa = 6.2EE888 pKa = 4.25MNEE891 pKa = 3.74QALINGLTPAADD903 pKa = 3.41HH904 pKa = 6.43FSDD907 pKa = 3.84YY908 pKa = 11.3ARR910 pKa = 11.84YY911 pKa = 9.33YY912 pKa = 11.33LNATHH917 pKa = 7.57RR918 pKa = 11.84NKK920 pKa = 10.15QDD922 pKa = 3.49LARR925 pKa = 11.84MLGVYY930 pKa = 8.09SEE932 pKa = 4.41RR933 pKa = 11.84TGLTTVSMSSRR944 pKa = 11.84PLKK947 pKa = 9.21EE948 pKa = 3.45VHH950 pKa = 6.39MLVPSLIQKK959 pKa = 7.87KK960 pKa = 7.88TYY962 pKa = 10.02TEE964 pKa = 4.06LGYY967 pKa = 8.7KK968 pKa = 8.76TSTYY972 pKa = 10.65AGCQGITAPKK982 pKa = 9.78VQVLLNSDD990 pKa = 3.51TTLCTDD996 pKa = 3.14KK997 pKa = 11.18VLYY1000 pKa = 8.68TALSRR1005 pKa = 11.84AVHH1008 pKa = 6.57SIHH1011 pKa = 7.45FINTSASSAAFWDD1024 pKa = 3.89KK1025 pKa = 11.56LNATPYY1031 pKa = 10.8LKK1033 pKa = 9.91TFLSTVRR1040 pKa = 11.84EE1041 pKa = 3.95QSLASAKK1048 pKa = 9.88PRR1050 pKa = 11.84EE1051 pKa = 4.3EE1052 pKa = 4.46GPTPVAAPKK1061 pKa = 8.84THH1063 pKa = 6.03VPVEE1067 pKa = 3.98NSNLCLEE1074 pKa = 4.52GAVEE1078 pKa = 4.65KK1079 pKa = 10.84LLEE1082 pKa = 4.12KK1083 pKa = 10.18HH1084 pKa = 6.3DD1085 pKa = 4.3RR1086 pKa = 11.84EE1087 pKa = 4.75IFRR1090 pKa = 11.84ADD1092 pKa = 3.47HH1093 pKa = 5.6GHH1095 pKa = 5.92SNCVQTDD1102 pKa = 3.16NPTIQLSSHH1111 pKa = 5.27QQAKK1115 pKa = 10.41DD1116 pKa = 3.39QTLLWATIEE1125 pKa = 3.96ARR1127 pKa = 11.84LKK1129 pKa = 10.57ISTPEE1134 pKa = 3.86RR1135 pKa = 11.84NLAEE1139 pKa = 4.0YY1140 pKa = 10.8LMKK1143 pKa = 10.59RR1144 pKa = 11.84EE1145 pKa = 4.1VGDD1148 pKa = 4.1VLWLHH1153 pKa = 6.41YY1154 pKa = 10.09KK1155 pKa = 10.41NAMNLPEE1162 pKa = 4.24EE1163 pKa = 5.04PIPFEE1168 pKa = 3.6QTLWDD1173 pKa = 3.6TCAEE1177 pKa = 4.27EE1178 pKa = 4.16VQKK1181 pKa = 9.91TYY1183 pKa = 10.87LSKK1186 pKa = 10.58PMHH1189 pKa = 5.62MLKK1192 pKa = 10.46NGEE1195 pKa = 4.27LRR1197 pKa = 11.84QSPDD1201 pKa = 2.83FHH1203 pKa = 7.62KK1204 pKa = 10.68NTIALFLKK1212 pKa = 9.3SQWVKK1217 pKa = 11.13KK1218 pKa = 7.9MEE1220 pKa = 4.25KK1221 pKa = 10.35LGADD1225 pKa = 3.86KK1226 pKa = 10.45IKK1228 pKa = 10.44PGQTIASFQQAAVMLYY1244 pKa = 8.76GTMARR1249 pKa = 11.84YY1250 pKa = 7.15MRR1252 pKa = 11.84RR1253 pKa = 11.84MRR1255 pKa = 11.84EE1256 pKa = 4.07VFQPTHH1262 pKa = 5.78VFINCEE1268 pKa = 3.74QSPEE1272 pKa = 4.21GMSDD1276 pKa = 3.54WIKK1279 pKa = 11.18NEE1281 pKa = 3.8WNFKK1285 pKa = 9.36GNAYY1289 pKa = 10.52ANDD1292 pKa = 3.62FTQFDD1297 pKa = 3.87QSQDD1301 pKa = 2.96GAMLQFEE1308 pKa = 4.37ILKK1311 pKa = 9.93AKK1313 pKa = 9.62HH1314 pKa = 5.9HH1315 pKa = 6.67SIPEE1319 pKa = 4.15WVLEE1323 pKa = 4.41GYY1325 pKa = 11.04LDD1327 pKa = 3.45IKK1329 pKa = 9.94TNAHH1333 pKa = 5.84VFTGTLGIMRR1343 pKa = 11.84LTGEE1347 pKa = 4.77GPTFDD1352 pKa = 6.01ANTEE1356 pKa = 4.01CNIAYY1361 pKa = 7.09THH1363 pKa = 6.8LRR1365 pKa = 11.84FNVPKK1370 pKa = 9.79GTAQAYY1376 pKa = 10.26AGDD1379 pKa = 4.44DD1380 pKa = 3.65SVLACIPEE1388 pKa = 3.98EE1389 pKa = 4.06RR1390 pKa = 11.84EE1391 pKa = 4.12SFKK1394 pKa = 9.99MWKK1397 pKa = 10.0NKK1399 pKa = 10.47LEE1401 pKa = 4.09LTAKK1405 pKa = 10.35PEE1407 pKa = 4.18YY1408 pKa = 9.25FPQMQGEE1415 pKa = 4.32WAQFCGFLFTPLGALKK1431 pKa = 10.5EE1432 pKa = 4.12PLKK1435 pKa = 10.24MLAVVEE1441 pKa = 4.12LAKK1444 pKa = 10.58QRR1446 pKa = 11.84GEE1448 pKa = 3.86LKK1450 pKa = 10.82KK1451 pKa = 10.79NMRR1454 pKa = 11.84SYY1456 pKa = 11.68ALDD1459 pKa = 3.21VLLNYY1464 pKa = 9.65QHH1466 pKa = 7.07GDD1468 pKa = 3.14ALQHH1472 pKa = 5.68VFSEE1476 pKa = 4.61KK1477 pKa = 10.03QLEE1480 pKa = 4.05AHH1482 pKa = 6.41QITVRR1487 pKa = 11.84NLIKK1491 pKa = 10.74FGGSSILEE1499 pKa = 4.1NCC1501 pKa = 4.35

Molecular weight:
169.64 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|G3LHV7|G3LHV7_9VIRU Movement protein TGBp3 OS=Tamus red mosaic virus OX=1081702 PE=3 SV=1
MM1 pKa = 7.78SGRR4 pKa = 11.84QVHH7 pKa = 5.99LTPPVDD13 pKa = 3.45HH14 pKa = 7.02SNSVLALVVGVSIALVVFSITRR36 pKa = 11.84NTSPHH41 pKa = 6.35VGDD44 pKa = 5.1NIHH47 pKa = 6.37SLPHH51 pKa = 5.77GGCYY55 pKa = 9.65KK56 pKa = 10.88DD57 pKa = 3.65GTKK60 pKa = 10.19SIYY63 pKa = 9.53YY64 pKa = 8.28GKK66 pKa = 9.34PGNSVRR72 pKa = 11.84QPARR76 pKa = 11.84GAALVLVIFLPILIYY91 pKa = 10.44AIHH94 pKa = 6.61KK95 pKa = 9.64FEE97 pKa = 4.16SRR99 pKa = 11.84RR100 pKa = 11.84TRR102 pKa = 11.84VSACGHH108 pKa = 6.15IACPSGGATQHH119 pKa = 6.06

Molecular weight:
12.65 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2135

64

1501

427.0

47.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.244 ± 0.383

1.358 ± 0.241

4.496 ± 0.549

7.026 ± 1.141

4.871 ± 0.469

5.386 ± 1.108

3.232 ± 0.837

4.356 ± 0.595

6.511 ± 0.787

9.836 ± 0.754

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.248 ± 0.537

4.309 ± 0.228

5.574 ± 0.957

4.169 ± 0.887

4.309 ± 0.348

7.119 ± 1.055

6.792 ± 0.658

6.37 ± 1.371

1.311 ± 0.49

2.436 ± 0.304

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski