Amino acid dipepetide frequency for Mycobacterium phage WillSterrel

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.53AlaAla: 15.53 ± 1.914
1.285AlaCys: 1.285 ± 0.304
6.694AlaAsp: 6.694 ± 0.625
7.122AlaGlu: 7.122 ± 0.6
3.427AlaPhe: 3.427 ± 0.431
10.014AlaGly: 10.014 ± 1.315
2.731AlaHis: 2.731 ± 0.402
4.445AlaIle: 4.445 ± 0.539
4.177AlaLys: 4.177 ± 0.478
7.658AlaLeu: 7.658 ± 0.801
2.57AlaMet: 2.57 ± 0.401
2.785AlaAsn: 2.785 ± 0.412
4.82AlaPro: 4.82 ± 0.578
3.267AlaGln: 3.267 ± 0.383
8.14AlaArg: 8.14 ± 0.753
4.927AlaSer: 4.927 ± 0.626
6.426AlaThr: 6.426 ± 0.566
7.069AlaVal: 7.069 ± 0.701
2.785AlaTrp: 2.785 ± 0.442
2.624AlaTyr: 2.624 ± 0.366
0.0AlaXaa: 0.0 ± 0.0
Cys
0.803CysAla: 0.803 ± 0.25
0.107CysCys: 0.107 ± 0.076
1.339CysAsp: 1.339 ± 0.323
0.75CysGlu: 0.75 ± 0.21
0.321CysPhe: 0.321 ± 0.118
2.088CysGly: 2.088 ± 0.483
0.268CysHis: 0.268 ± 0.115
0.375CysIle: 0.375 ± 0.143
0.482CysLys: 0.482 ± 0.193
0.536CysLeu: 0.536 ± 0.225
0.107CysMet: 0.107 ± 0.073
0.428CysAsn: 0.428 ± 0.153
0.964CysPro: 0.964 ± 0.255
0.321CysGln: 0.321 ± 0.123
1.071CysArg: 1.071 ± 0.289
1.071CysSer: 1.071 ± 0.288
0.857CysThr: 0.857 ± 0.207
0.91CysVal: 0.91 ± 0.203
0.268CysTrp: 0.268 ± 0.111
0.161CysTyr: 0.161 ± 0.096
0.0CysXaa: 0.0 ± 0.0
Asp
7.283AspAla: 7.283 ± 0.644
1.017AspCys: 1.017 ± 0.209
4.445AspAsp: 4.445 ± 0.627
2.731AspGlu: 2.731 ± 0.387
1.66AspPhe: 1.66 ± 0.253
6.854AspGly: 6.854 ± 0.553
1.392AspHis: 1.392 ± 0.286
2.249AspIle: 2.249 ± 0.361
1.607AspLys: 1.607 ± 0.29
6.158AspLeu: 6.158 ± 0.56
1.071AspMet: 1.071 ± 0.25
1.607AspAsn: 1.607 ± 0.336
4.659AspPro: 4.659 ± 0.638
2.035AspGln: 2.035 ± 0.282
4.98AspArg: 4.98 ± 0.723
3.267AspSer: 3.267 ± 0.492
3.909AspThr: 3.909 ± 0.506
4.07AspVal: 4.07 ± 0.512
1.553AspTrp: 1.553 ± 0.287
1.714AspTyr: 1.714 ± 0.301
0.0AspXaa: 0.0 ± 0.0
Glu
5.73GluAla: 5.73 ± 0.543
1.017GluCys: 1.017 ± 0.237
2.731GluAsp: 2.731 ± 0.32
2.731GluGlu: 2.731 ± 0.4
2.463GluPhe: 2.463 ± 0.411
2.945GluGly: 2.945 ± 0.422
1.339GluHis: 1.339 ± 0.317
1.874GluIle: 1.874 ± 0.296
1.981GluLys: 1.981 ± 0.306
4.873GluLeu: 4.873 ± 0.558
1.499GluMet: 1.499 ± 0.274
2.249GluAsn: 2.249 ± 0.304
3.052GluPro: 3.052 ± 0.487
3.213GluGln: 3.213 ± 0.398
4.873GluArg: 4.873 ± 0.53
3.052GluSer: 3.052 ± 0.474
3.749GluThr: 3.749 ± 0.51
3.856GluVal: 3.856 ± 0.55
1.553GluTrp: 1.553 ± 0.241
1.66GluTyr: 1.66 ± 0.321
0.0GluXaa: 0.0 ± 0.0
Phe
3.481PheAla: 3.481 ± 0.432
0.107PheCys: 0.107 ± 0.075
2.356PheAsp: 2.356 ± 0.416
1.767PheGlu: 1.767 ± 0.31
0.857PhePhe: 0.857 ± 0.235
2.945PheGly: 2.945 ± 0.597
0.321PheHis: 0.321 ± 0.126
1.446PheIle: 1.446 ± 0.342
0.803PheLys: 0.803 ± 0.211
2.303PheLeu: 2.303 ± 0.305
0.803PheMet: 0.803 ± 0.239
1.178PheAsn: 1.178 ± 0.323
1.66PhePro: 1.66 ± 0.348
0.964PheGln: 0.964 ± 0.281
1.66PheArg: 1.66 ± 0.279
1.446PheSer: 1.446 ± 0.266
2.731PheThr: 2.731 ± 0.379
2.356PheVal: 2.356 ± 0.288
0.696PheTrp: 0.696 ± 0.166
0.91PheTyr: 0.91 ± 0.226
0.0PheXaa: 0.0 ± 0.0
Gly
8.354GlyAla: 8.354 ± 1.077
1.392GlyCys: 1.392 ± 0.274
5.944GlyAsp: 5.944 ± 0.52
3.856GlyGlu: 3.856 ± 0.46
3.052GlyPhe: 3.052 ± 0.402
10.657GlyGly: 10.657 ± 1.908
2.196GlyHis: 2.196 ± 0.315
3.749GlyIle: 3.749 ± 0.577
2.41GlyLys: 2.41 ± 0.369
6.212GlyLeu: 6.212 ± 0.58
2.57GlyMet: 2.57 ± 0.449
3.106GlyAsn: 3.106 ± 0.425
4.284GlyPro: 4.284 ± 0.568
2.196GlyGln: 2.196 ± 0.573
5.194GlyArg: 5.194 ± 0.559
6.105GlySer: 6.105 ± 0.867
6.801GlyThr: 6.801 ± 0.786
5.409GlyVal: 5.409 ± 0.611
2.463GlyTrp: 2.463 ± 0.357
2.303GlyTyr: 2.303 ± 0.371
0.0GlyXaa: 0.0 ± 0.0
His
1.714HisAla: 1.714 ± 0.286
0.482HisCys: 0.482 ± 0.174
1.071HisAsp: 1.071 ± 0.24
1.392HisGlu: 1.392 ± 0.279
0.214HisPhe: 0.214 ± 0.099
1.874HisGly: 1.874 ± 0.268
0.696HisHis: 0.696 ± 0.191
1.339HisIle: 1.339 ± 0.259
0.643HisLys: 0.643 ± 0.195
1.607HisLeu: 1.607 ± 0.354
0.536HisMet: 0.536 ± 0.141
0.857HisAsn: 0.857 ± 0.2
1.446HisPro: 1.446 ± 0.222
1.071HisGln: 1.071 ± 0.258
2.303HisArg: 2.303 ± 0.375
1.071HisSer: 1.071 ± 0.216
1.339HisThr: 1.339 ± 0.373
1.392HisVal: 1.392 ± 0.364
0.589HisTrp: 0.589 ± 0.157
0.643HisTyr: 0.643 ± 0.158
0.0HisXaa: 0.0 ± 0.0
Ile
4.927IleAla: 4.927 ± 0.46
0.696IleCys: 0.696 ± 0.273
3.374IleAsp: 3.374 ± 0.394
3.534IleGlu: 3.534 ± 0.374
0.803IlePhe: 0.803 ± 0.211
3.856IleGly: 3.856 ± 0.487
1.339IleHis: 1.339 ± 0.235
1.499IleIle: 1.499 ± 0.3
1.392IleLys: 1.392 ± 0.274
2.196IleLeu: 2.196 ± 0.416
0.321IleMet: 0.321 ± 0.112
2.142IleAsn: 2.142 ± 0.287
2.785IlePro: 2.785 ± 0.298
1.499IleGln: 1.499 ± 0.249
2.41IleArg: 2.41 ± 0.302
2.356IleSer: 2.356 ± 0.434
3.588IleThr: 3.588 ± 0.392
2.838IleVal: 2.838 ± 0.395
0.857IleTrp: 0.857 ± 0.191
0.643IleTyr: 0.643 ± 0.177
0.0IleXaa: 0.0 ± 0.0
Lys
4.23LysAla: 4.23 ± 0.44
0.214LysCys: 0.214 ± 0.098
1.714LysAsp: 1.714 ± 0.255
1.232LysGlu: 1.232 ± 0.257
1.232LysPhe: 1.232 ± 0.226
2.785LysGly: 2.785 ± 0.315
0.91LysHis: 0.91 ± 0.252
1.125LysIle: 1.125 ± 0.242
1.392LysLys: 1.392 ± 0.375
2.249LysLeu: 2.249 ± 0.402
0.428LysMet: 0.428 ± 0.148
0.964LysAsn: 0.964 ± 0.253
1.928LysPro: 1.928 ± 0.317
1.66LysGln: 1.66 ± 0.25
2.41LysArg: 2.41 ± 0.392
2.088LysSer: 2.088 ± 0.337
1.981LysThr: 1.981 ± 0.384
2.41LysVal: 2.41 ± 0.396
0.696LysTrp: 0.696 ± 0.263
1.125LysTyr: 1.125 ± 0.264
0.0LysXaa: 0.0 ± 0.0
Leu
7.925LeuAla: 7.925 ± 0.852
0.803LeuCys: 0.803 ± 0.281
5.034LeuAsp: 5.034 ± 0.564
3.32LeuGlu: 3.32 ± 0.413
2.196LeuPhe: 2.196 ± 0.26
5.087LeuGly: 5.087 ± 0.472
1.125LeuHis: 1.125 ± 0.265
3.267LeuIle: 3.267 ± 0.448
1.821LeuLys: 1.821 ± 0.305
4.498LeuLeu: 4.498 ± 0.499
1.66LeuMet: 1.66 ± 0.337
2.463LeuAsn: 2.463 ± 0.36
5.409LeuPro: 5.409 ± 0.616
3.052LeuGln: 3.052 ± 0.425
5.248LeuArg: 5.248 ± 0.649
5.462LeuSer: 5.462 ± 0.486
5.623LeuThr: 5.623 ± 0.527
4.927LeuVal: 4.927 ± 0.561
1.339LeuTrp: 1.339 ± 0.36
2.249LeuTyr: 2.249 ± 0.397
0.0LeuXaa: 0.0 ± 0.0
Met
2.624MetAla: 2.624 ± 0.472
0.214MetCys: 0.214 ± 0.099
1.017MetAsp: 1.017 ± 0.238
1.017MetGlu: 1.017 ± 0.187
0.589MetPhe: 0.589 ± 0.154
1.874MetGly: 1.874 ± 0.314
0.107MetHis: 0.107 ± 0.069
0.964MetIle: 0.964 ± 0.245
0.857MetLys: 0.857 ± 0.216
1.928MetLeu: 1.928 ± 0.263
0.643MetMet: 0.643 ± 0.203
1.232MetAsn: 1.232 ± 0.26
0.964MetPro: 0.964 ± 0.191
0.214MetGln: 0.214 ± 0.098
1.607MetArg: 1.607 ± 0.285
2.838MetSer: 2.838 ± 0.377
1.821MetThr: 1.821 ± 0.288
1.178MetVal: 1.178 ± 0.295
0.268MetTrp: 0.268 ± 0.131
0.161MetTyr: 0.161 ± 0.089
0.0MetXaa: 0.0 ± 0.0
Asn
3.695AsnAla: 3.695 ± 0.346
0.321AsnCys: 0.321 ± 0.12
1.714AsnAsp: 1.714 ± 0.292
1.607AsnGlu: 1.607 ± 0.337
1.017AsnPhe: 1.017 ± 0.284
4.23AsnGly: 4.23 ± 0.741
1.017AsnHis: 1.017 ± 0.222
1.66AsnIle: 1.66 ± 0.443
0.964AsnLys: 0.964 ± 0.187
2.303AsnLeu: 2.303 ± 0.302
0.589AsnMet: 0.589 ± 0.16
1.553AsnAsn: 1.553 ± 0.359
2.624AsnPro: 2.624 ± 0.332
0.91AsnGln: 0.91 ± 0.312
2.624AsnArg: 2.624 ± 0.417
1.767AsnSer: 1.767 ± 0.306
1.714AsnThr: 1.714 ± 0.31
1.928AsnVal: 1.928 ± 0.369
0.643AsnTrp: 0.643 ± 0.197
1.071AsnTyr: 1.071 ± 0.212
0.0AsnXaa: 0.0 ± 0.0
Pro
5.462ProAla: 5.462 ± 0.567
0.75ProCys: 0.75 ± 0.19
4.07ProAsp: 4.07 ± 0.531
4.712ProGlu: 4.712 ± 0.517
1.767ProPhe: 1.767 ± 0.35
5.998ProGly: 5.998 ± 0.689
1.499ProHis: 1.499 ± 0.293
1.607ProIle: 1.607 ± 0.315
2.035ProLys: 2.035 ± 0.319
4.391ProLeu: 4.391 ± 0.481
1.607ProMet: 1.607 ± 0.31
1.981ProAsn: 1.981 ± 0.296
3.963ProPro: 3.963 ± 0.65
2.196ProGln: 2.196 ± 0.39
3.427ProArg: 3.427 ± 0.481
3.32ProSer: 3.32 ± 0.472
3.534ProThr: 3.534 ± 0.449
4.927ProVal: 4.927 ± 0.483
0.964ProTrp: 0.964 ± 0.25
1.125ProTyr: 1.125 ± 0.212
0.0ProXaa: 0.0 ± 0.0
Gln
4.177GlnAla: 4.177 ± 0.566
0.161GlnCys: 0.161 ± 0.088
1.553GlnAsp: 1.553 ± 0.268
1.392GlnGlu: 1.392 ± 0.273
1.178GlnPhe: 1.178 ± 0.255
2.517GlnGly: 2.517 ± 0.472
0.75GlnHis: 0.75 ± 0.194
1.874GlnIle: 1.874 ± 0.332
1.339GlnLys: 1.339 ± 0.249
2.892GlnLeu: 2.892 ± 0.325
0.696GlnMet: 0.696 ± 0.173
0.803GlnAsn: 0.803 ± 0.259
2.463GlnPro: 2.463 ± 0.377
1.392GlnGln: 1.392 ± 0.356
3.052GlnArg: 3.052 ± 0.444
2.303GlnSer: 2.303 ± 0.329
1.66GlnThr: 1.66 ± 0.279
2.57GlnVal: 2.57 ± 0.398
0.536GlnTrp: 0.536 ± 0.243
0.91GlnTyr: 0.91 ± 0.215
0.0GlnXaa: 0.0 ± 0.0
Arg
6.694ArgAla: 6.694 ± 0.667
1.499ArgCys: 1.499 ± 0.411
4.927ArgAsp: 4.927 ± 0.573
5.034ArgGlu: 5.034 ± 0.573
2.356ArgPhe: 2.356 ± 0.397
4.177ArgGly: 4.177 ± 0.417
1.446ArgHis: 1.446 ± 0.396
4.391ArgIle: 4.391 ± 0.565
2.624ArgLys: 2.624 ± 0.407
4.873ArgLeu: 4.873 ± 0.63
2.356ArgMet: 2.356 ± 0.402
2.303ArgAsn: 2.303 ± 0.367
3.641ArgPro: 3.641 ± 0.375
2.356ArgGln: 2.356 ± 0.482
6.962ArgArg: 6.962 ± 1.033
4.445ArgSer: 4.445 ± 0.597
3.267ArgThr: 3.267 ± 0.471
5.409ArgVal: 5.409 ± 0.674
1.981ArgTrp: 1.981 ± 0.347
2.142ArgTyr: 2.142 ± 0.326
0.0ArgXaa: 0.0 ± 0.0
Ser
7.497SerAla: 7.497 ± 1.186
0.375SerCys: 0.375 ± 0.117
4.177SerAsp: 4.177 ± 0.489
3.427SerGlu: 3.427 ± 0.452
2.088SerPhe: 2.088 ± 0.431
6.051SerGly: 6.051 ± 0.753
1.339SerHis: 1.339 ± 0.247
2.785SerIle: 2.785 ± 0.454
2.517SerLys: 2.517 ± 0.368
3.749SerLeu: 3.749 ± 0.442
1.339SerMet: 1.339 ± 0.249
2.196SerAsn: 2.196 ± 0.383
3.159SerPro: 3.159 ± 0.317
1.66SerGln: 1.66 ± 0.246
3.749SerArg: 3.749 ± 0.396
3.909SerSer: 3.909 ± 0.664
3.749SerThr: 3.749 ± 0.437
4.552SerVal: 4.552 ± 0.51
1.071SerTrp: 1.071 ± 0.229
1.339SerTyr: 1.339 ± 0.232
0.0SerXaa: 0.0 ± 0.0
Thr
6.64ThrAla: 6.64 ± 0.642
0.857ThrCys: 0.857 ± 0.25
4.07ThrAsp: 4.07 ± 0.54
3.749ThrGlu: 3.749 ± 0.437
1.874ThrPhe: 1.874 ± 0.271
6.319ThrGly: 6.319 ± 0.509
1.499ThrHis: 1.499 ± 0.246
3.32ThrIle: 3.32 ± 0.453
2.035ThrLys: 2.035 ± 0.344
4.605ThrLeu: 4.605 ± 0.542
1.285ThrMet: 1.285 ± 0.24
2.249ThrAsn: 2.249 ± 0.408
4.927ThrPro: 4.927 ± 0.581
2.196ThrGln: 2.196 ± 0.333
3.963ThrArg: 3.963 ± 0.477
3.909ThrSer: 3.909 ± 0.474
4.498ThrThr: 4.498 ± 0.627
5.676ThrVal: 5.676 ± 0.721
1.178ThrTrp: 1.178 ± 0.284
1.66ThrTyr: 1.66 ± 0.313
0.0ThrXaa: 0.0 ± 0.0
Val
7.551ValAla: 7.551 ± 0.566
1.339ValCys: 1.339 ± 0.285
5.194ValAsp: 5.194 ± 0.564
4.445ValGlu: 4.445 ± 0.487
2.142ValPhe: 2.142 ± 0.362
4.927ValGly: 4.927 ± 0.566
1.285ValHis: 1.285 ± 0.267
2.678ValIle: 2.678 ± 0.444
2.088ValLys: 2.088 ± 0.327
5.516ValLeu: 5.516 ± 0.74
0.964ValMet: 0.964 ± 0.172
2.196ValAsn: 2.196 ± 0.372
4.284ValPro: 4.284 ± 0.373
2.785ValGln: 2.785 ± 0.337
4.927ValArg: 4.927 ± 0.622
4.605ValSer: 4.605 ± 0.548
5.462ValThr: 5.462 ± 0.476
5.73ValVal: 5.73 ± 0.683
1.821ValTrp: 1.821 ± 0.29
1.125ValTyr: 1.125 ± 0.249
0.0ValXaa: 0.0 ± 0.0
Trp
2.035TrpAla: 2.035 ± 0.274
0.161TrpCys: 0.161 ± 0.098
1.339TrpAsp: 1.339 ± 0.208
1.071TrpGlu: 1.071 ± 0.272
0.803TrpPhe: 0.803 ± 0.224
1.232TrpGly: 1.232 ± 0.259
0.428TrpHis: 0.428 ± 0.136
1.125TrpIle: 1.125 ± 0.198
0.75TrpLys: 0.75 ± 0.184
1.874TrpLeu: 1.874 ± 0.337
0.857TrpMet: 0.857 ± 0.249
0.696TrpAsn: 0.696 ± 0.224
1.017TrpPro: 1.017 ± 0.258
0.857TrpGln: 0.857 ± 0.266
1.981TrpArg: 1.981 ± 0.361
1.607TrpSer: 1.607 ± 0.371
1.981TrpThr: 1.981 ± 0.316
1.553TrpVal: 1.553 ± 0.419
1.125TrpTrp: 1.125 ± 0.212
0.268TrpTyr: 0.268 ± 0.126
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.196TyrAla: 2.196 ± 0.387
0.428TyrCys: 0.428 ± 0.181
1.499TyrAsp: 1.499 ± 0.354
1.553TyrGlu: 1.553 ± 0.288
0.75TyrPhe: 0.75 ± 0.237
1.821TyrGly: 1.821 ± 0.382
0.589TyrHis: 0.589 ± 0.171
1.285TyrIle: 1.285 ± 0.277
0.857TyrLys: 0.857 ± 0.302
2.088TyrLeu: 2.088 ± 0.3
0.161TyrMet: 0.161 ± 0.096
0.964TyrAsn: 0.964 ± 0.244
1.285TyrPro: 1.285 ± 0.269
0.375TyrGln: 0.375 ± 0.131
2.303TyrArg: 2.303 ± 0.396
1.178TyrSer: 1.178 ± 0.259
1.821TyrThr: 1.821 ± 0.317
2.303TyrVal: 2.303 ± 0.307
0.375TyrTrp: 0.375 ± 0.14
0.536TyrTyr: 0.536 ± 0.156
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 104 proteins (18675 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski