Handroanthus impetiginosus

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Lamiales; Bignoniaceae; Crescentiina; Tabebuia alliance;

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 29726 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2G9HGW1|A0A2G9HGW1_9LAMI AP2/ERF domain-containing protein OS=Handroanthus impetiginosus OX=429701 GN=CDL12_10560 PE=4 SV=1
MM1 pKa = 7.47ASSMVAEE8 pKa = 4.21NWDD11 pKa = 3.67YY12 pKa = 12.1ADD14 pKa = 3.62GHH16 pKa = 6.85DD17 pKa = 4.07GSEE20 pKa = 4.19SLEE23 pKa = 3.72LSQINQNLLMSLLDD37 pKa = 3.39EE38 pKa = 4.43TQIDD42 pKa = 3.82DD43 pKa = 4.58CDD45 pKa = 4.06DD46 pKa = 3.37EE47 pKa = 5.69RR48 pKa = 11.84LINVIRR54 pKa = 11.84SLEE57 pKa = 3.94AEE59 pKa = 3.8IDD61 pKa = 3.36FDD63 pKa = 4.04GQHH66 pKa = 7.06AIDD69 pKa = 3.56NAMWEE74 pKa = 4.31SDD76 pKa = 4.38LVDD79 pKa = 4.0CQSSNDD85 pKa = 3.75EE86 pKa = 4.64LNGQDD91 pKa = 4.29RR92 pKa = 11.84SLPHH96 pKa = 7.52DD97 pKa = 4.08NLDD100 pKa = 3.8LHH102 pKa = 6.27WMDD105 pKa = 4.46MEE107 pKa = 5.23TIPSSPSGAMGNWYY121 pKa = 8.85MDD123 pKa = 3.79HH124 pKa = 6.84NGQGIMGSGNEE135 pKa = 3.93FGGAKK140 pKa = 10.0NYY142 pKa = 10.86SNFWNGTPLEE152 pKa = 4.21EE153 pKa = 4.42QDD155 pKa = 5.04YY156 pKa = 10.83GSLWHH161 pKa = 6.05EE162 pKa = 4.43TNVIASDD169 pKa = 3.33

Molecular weight: 18.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2G9GHS7|A0A2G9GHS7_9LAMI Glutathione S-transferase OS=Handroanthus impetiginosus OX=429701 GN=CDL12_22715 PE=3 SV=1
MM1 pKa = 7.13QLRR4 pKa = 11.84RR5 pKa = 11.84LPRR8 pKa = 11.84HH9 pKa = 5.79PLRR12 pKa = 11.84PLSPTILPRR21 pKa = 11.84MRR23 pKa = 11.84HH24 pKa = 4.68LRR26 pKa = 11.84NNRR29 pKa = 11.84FHH31 pKa = 5.87FRR33 pKa = 11.84KK34 pKa = 9.45RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84LPIQQSLRR46 pKa = 11.84PPTKK50 pKa = 10.28RR51 pKa = 11.84KK52 pKa = 5.89PTKK55 pKa = 9.44HH56 pKa = 4.78FHH58 pKa = 6.3RR59 pKa = 11.84PRR61 pKa = 11.84TRR63 pKa = 11.84RR64 pKa = 11.84SKK66 pKa = 9.69SEE68 pKa = 3.46HH69 pKa = 6.55RR70 pKa = 11.84NFHH73 pKa = 6.72PKK75 pKa = 8.95LQSRR79 pKa = 11.84RR80 pKa = 11.84RR81 pKa = 11.84HH82 pKa = 5.18GFNSCFVPVV91 pKa = 3.48

Molecular weight: 11.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

29726

0

29726

11135101

26

4252

374.6

41.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.658 ± 0.014

1.877 ± 0.007

5.236 ± 0.009

6.457 ± 0.017

4.343 ± 0.01

6.454 ± 0.014

2.357 ± 0.007

5.582 ± 0.012

6.209 ± 0.014

9.789 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.445 ± 0.006

4.676 ± 0.009

4.943 ± 0.015

3.582 ± 0.01

5.27 ± 0.01

8.765 ± 0.017

4.788 ± 0.006

6.37 ± 0.01

1.284 ± 0.004

2.912 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski