Streptococcus phage Javan362

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6B4N9|A0A4D6B4N9_9CAUD Terminase small subunit OS=Streptococcus phage Javan362 OX=2548125 GN=Javan362_0028 PE=4 SV=1
MM1 pKa = 7.56FDD3 pKa = 3.45YY4 pKa = 10.76DD5 pKa = 4.53RR6 pKa = 11.84DD7 pKa = 3.62MMQPPEE13 pKa = 4.2EE14 pKa = 4.49RR15 pKa = 11.84EE16 pKa = 3.79EE17 pKa = 4.45LDD19 pKa = 3.12PSEE22 pKa = 4.42YY23 pKa = 11.03VDD25 pKa = 3.17IGCGRR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84YY33 pKa = 10.43VGDD36 pKa = 3.54EE37 pKa = 3.95VV38 pKa = 3.65

Molecular weight: 4.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6B3W4|A0A4D6B3W4_9CAUD Uncharacterized protein OS=Streptococcus phage Javan362 OX=2548125 GN=Javan362_0053 PE=4 SV=1
MM1 pKa = 8.39IEE3 pKa = 4.15EE4 pKa = 4.07LHH6 pKa = 7.91AEE8 pKa = 3.86IDD10 pKa = 3.47RR11 pKa = 11.84WRR13 pKa = 11.84SDD15 pKa = 3.61YY16 pKa = 11.09IHH18 pKa = 7.38LGRR21 pKa = 11.84EE22 pKa = 3.52LGKK25 pKa = 10.36IINEE29 pKa = 3.98QQDD32 pKa = 3.94IILKK36 pKa = 9.22LQNKK40 pKa = 7.15NRR42 pKa = 11.84RR43 pKa = 11.84LKK45 pKa = 10.66RR46 pKa = 11.84EE47 pKa = 3.36NWNLKK52 pKa = 7.23KK53 pKa = 9.51TKK55 pKa = 9.55GRR57 pKa = 11.84KK58 pKa = 8.57KK59 pKa = 10.52

Molecular weight: 7.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

11888

38

1399

208.6

23.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.032 ± 0.696

0.698 ± 0.141

6.065 ± 0.33

8.302 ± 0.339

4.038 ± 0.281

6.376 ± 0.388

1.329 ± 0.156

6.519 ± 0.247

8.698 ± 0.318

8.328 ± 0.29

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.624 ± 0.168

5.375 ± 0.238

2.784 ± 0.217

4.416 ± 0.259

4.669 ± 0.293

6.141 ± 0.327

5.728 ± 0.281

6.031 ± 0.269

1.102 ± 0.146

3.743 ± 0.289
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski