Klebsiella phage KMI7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; unclassified Tevenvirinae

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 187 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B9NHD1|A0A5B9NHD1_9CAUD Uncharacterized protein OS=Klebsiella phage KMI7 OX=2601618 GN=KMI7_117 PE=4 SV=1
MM1 pKa = 7.61NIDD4 pKa = 3.47FCKK7 pKa = 10.91AEE9 pKa = 4.32IKK11 pKa = 10.77ADD13 pKa = 3.42MDD15 pKa = 3.85EE16 pKa = 4.29EE17 pKa = 4.46PKK19 pKa = 10.41FDD21 pKa = 4.04SPHH24 pKa = 5.84SSRR27 pKa = 11.84WIVAVDD33 pKa = 3.36DD34 pKa = 4.18EE35 pKa = 5.63GYY37 pKa = 8.7VTVLSRR43 pKa = 11.84PFIHH47 pKa = 7.68DD48 pKa = 3.38AFFEE52 pKa = 4.47CGRR55 pKa = 11.84SAEE58 pKa = 4.86DD59 pKa = 3.0IGLPDD64 pKa = 4.94SIEE67 pKa = 4.16MPPGVYY73 pKa = 9.55EE74 pKa = 4.23WICGFEE80 pKa = 4.0QTTDD84 pKa = 2.87WEE86 pKa = 4.3TGYY89 pKa = 11.05VDD91 pKa = 3.11GWEE94 pKa = 4.47FYY96 pKa = 10.33PNEE99 pKa = 4.04STLLYY104 pKa = 10.44SWDD107 pKa = 3.46NGKK110 pKa = 10.21VNEE113 pKa = 4.72SGNQEE118 pKa = 4.11TQSDD122 pKa = 4.0NGSGSLL128 pKa = 4.04

Molecular weight: 14.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B9NBH6|A0A5B9NBH6_9CAUD Site-specific DNA-methyltransferase (adenine-specific) OS=Klebsiella phage KMI7 OX=2601618 GN=KMI7_39 PE=3 SV=1
MM1 pKa = 7.47KK2 pKa = 9.89IVHH5 pKa = 6.48RR6 pKa = 11.84SISEE10 pKa = 4.02LSQAEE15 pKa = 4.06KK16 pKa = 10.8VQIHH20 pKa = 6.27FKK22 pKa = 10.99FNDD25 pKa = 3.13LRR27 pKa = 11.84KK28 pKa = 9.89GYY30 pKa = 10.97AEE32 pKa = 3.98IARR35 pKa = 11.84EE36 pKa = 3.91HH37 pKa = 6.52GLDD40 pKa = 3.61NGDD43 pKa = 3.37IYY45 pKa = 11.3QVVQEE50 pKa = 4.32IEE52 pKa = 4.13RR53 pKa = 11.84IRR55 pKa = 11.84AFRR58 pKa = 11.84SANLPVKK65 pKa = 10.3NAMRR69 pKa = 11.84RR70 pKa = 11.84EE71 pKa = 4.19AIEE74 pKa = 3.6IHH76 pKa = 6.62RR77 pKa = 11.84KK78 pKa = 8.91RR79 pKa = 11.84VYY81 pKa = 10.08NLEE84 pKa = 3.92RR85 pKa = 11.84AKK87 pKa = 10.85RR88 pKa = 11.84PMKK91 pKa = 10.27KK92 pKa = 9.45EE93 pKa = 3.66KK94 pKa = 10.22KK95 pKa = 8.3PRR97 pKa = 11.84PFKK100 pKa = 10.65RR101 pKa = 11.84EE102 pKa = 3.75HH103 pKa = 6.82LNTSMALAFQDD114 pKa = 3.65AGIII118 pKa = 3.76

Molecular weight: 13.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

187

0

187

41015

44

1394

219.3

24.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.839 ± 0.211

1.136 ± 0.101

6.598 ± 0.114

6.827 ± 0.217

4.101 ± 0.139

6.517 ± 0.294

1.702 ± 0.1

6.698 ± 0.133

7.29 ± 0.268

7.234 ± 0.172

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.979 ± 0.107

5.632 ± 0.174

3.535 ± 0.091

3.167 ± 0.132

4.803 ± 0.14

6.095 ± 0.147

6.183 ± 0.322

6.866 ± 0.152

1.358 ± 0.068

4.44 ± 0.129
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski