Hortaea werneckii EXF-2000

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Dothideomycetidae; Mycosphaerellales; Teratosphaeriaceae; Hortaea; Hortaea werneckii

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15464 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Z5T4S0|A0A1Z5T4S0_HORWE Uncharacterized protein OS=Hortaea werneckii EXF-2000 OX=1157616 GN=BTJ68_08764 PE=3 SV=1
MM1 pKa = 7.36PRR3 pKa = 11.84SHH5 pKa = 6.56GRR7 pKa = 11.84RR8 pKa = 11.84TTAVLLTAAAAAATTVSALPEE29 pKa = 3.58IAITKK34 pKa = 10.04RR35 pKa = 11.84DD36 pKa = 3.7GEE38 pKa = 4.41LKK40 pKa = 10.14ASEE43 pKa = 4.14RR44 pKa = 11.84ARR46 pKa = 11.84GVEE49 pKa = 3.99KK50 pKa = 10.56RR51 pKa = 11.84AADD54 pKa = 3.55GNGTVEE60 pKa = 4.25TNVFDD65 pKa = 3.62VRR67 pKa = 11.84TWSTGGAYY75 pKa = 9.44YY76 pKa = 11.29ANVTVGTPPQPQVVILDD93 pKa = 3.84TGSSDD98 pKa = 4.8LYY100 pKa = 11.05FDD102 pKa = 4.79AADD105 pKa = 4.62APTCSLTGKK114 pKa = 8.35YY115 pKa = 9.41ACKK118 pKa = 10.43GGTFSPDD125 pKa = 2.75NSSTYY130 pKa = 10.34QVVDD134 pKa = 4.01PFPAFDD140 pKa = 3.8TLFGDD145 pKa = 4.74GSSATGEE152 pKa = 4.16YY153 pKa = 11.18GEE155 pKa = 4.52DD156 pKa = 3.16TVGIGDD162 pKa = 3.57VRR164 pKa = 11.84IEE166 pKa = 3.93NVQFGLANSLYY177 pKa = 8.45ITTGYY182 pKa = 11.18AVGLMGLGYY191 pKa = 10.34SYY193 pKa = 11.08IEE195 pKa = 5.15AIDD198 pKa = 4.02DD199 pKa = 4.39TEE201 pKa = 4.28DD202 pKa = 3.6LYY204 pKa = 10.93PNIPEE209 pKa = 4.05VLRR212 pKa = 11.84ATGVINSRR220 pKa = 11.84LYY222 pKa = 10.61SVYY225 pKa = 10.9LNDD228 pKa = 4.98LSDD231 pKa = 3.33ISGTILFGGIDD242 pKa = 3.61PSKK245 pKa = 9.79YY246 pKa = 9.3TGDD249 pKa = 3.53LTTLNILPDD258 pKa = 4.11VISGGISMFLTTVTDD273 pKa = 4.33LSITTDD279 pKa = 3.39GEE281 pKa = 4.35TADD284 pKa = 4.46LFSGGSTGLDD294 pKa = 2.85AYY296 pKa = 10.55DD297 pKa = 4.31AYY299 pKa = 10.88DD300 pKa = 3.4YY301 pKa = 11.25SLPVLLDD308 pKa = 3.19TGAAAWQVPSSRR320 pKa = 11.84YY321 pKa = 7.4TDD323 pKa = 3.95YY324 pKa = 10.84IAPAFPFVDD333 pKa = 3.3PLGYY337 pKa = 9.95CGCSHH342 pKa = 6.73RR343 pKa = 11.84QDD345 pKa = 5.6DD346 pKa = 3.9IQLTVTFGGEE356 pKa = 3.51IPIRR360 pKa = 11.84VPITDD365 pKa = 4.22FLVPIYY371 pKa = 9.62NTTTRR376 pKa = 11.84EE377 pKa = 4.2PIPYY381 pKa = 9.16TEE383 pKa = 5.93DD384 pKa = 3.38EE385 pKa = 4.55DD386 pKa = 4.27TCALLIVPAEE396 pKa = 3.81ATGYY400 pKa = 9.93GFQVLGDD407 pKa = 4.49AILRR411 pKa = 11.84SMYY414 pKa = 10.83VVFDD418 pKa = 4.61LDD420 pKa = 3.69NGQLSIAQAAEE431 pKa = 4.26DD432 pKa = 3.91NDD434 pKa = 4.12AGAGTSNPIPVPRR447 pKa = 11.84GPDD450 pKa = 3.74GIAAALSSASVGAAAEE466 pKa = 4.6DD467 pKa = 3.97YY468 pKa = 10.72RR469 pKa = 11.84SAEE472 pKa = 4.24STQTWSIAPLVTAASATGDD491 pKa = 3.87LSATTASRR499 pKa = 11.84TVGTATGSQAVPAGARR515 pKa = 11.84VSDD518 pKa = 4.14DD519 pKa = 3.67GVPGGDD525 pKa = 3.89DD526 pKa = 4.66GIGEE530 pKa = 4.96LIGVDD535 pKa = 5.59DD536 pKa = 6.72DD537 pKa = 6.85DD538 pKa = 7.88DD539 pKa = 7.42DD540 pKa = 7.55DD541 pKa = 7.05DD542 pKa = 7.16DD543 pKa = 7.12GDD545 pKa = 4.19GGSFGDD551 pKa = 4.51GGGEE555 pKa = 4.09ADD557 pKa = 4.45GSTSDD562 pKa = 4.06GSSSSSSSSGDD573 pKa = 3.29SSSTSSGAAAAVKK586 pKa = 10.4GGLSLGPDD594 pKa = 2.84WRR596 pKa = 11.84GWWVSGIVVLGVWVGMGVMLL616 pKa = 5.44

Molecular weight: 63.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Z5T3I2|A0A1Z5T3I2_HORWE MFS domain-containing protein OS=Hortaea werneckii EXF-2000 OX=1157616 GN=BTJ68_12399 PE=3 SV=1
MM1 pKa = 7.88PSHH4 pKa = 6.89KK5 pKa = 10.44SFRR8 pKa = 11.84TKK10 pKa = 10.65VKK12 pKa = 9.82LAKK15 pKa = 9.79AQKK18 pKa = 8.73QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.15WRR45 pKa = 11.84KK46 pKa = 7.38TRR48 pKa = 11.84IGII51 pKa = 4.0

Molecular weight: 6.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15464

0

15464

8020772

50

4939

518.7

57.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.125 ± 0.015

1.125 ± 0.006

5.735 ± 0.015

6.461 ± 0.019

3.539 ± 0.013

7.48 ± 0.021

2.378 ± 0.008

4.318 ± 0.013

4.859 ± 0.018

8.43 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.307 ± 0.008

3.646 ± 0.009

6.092 ± 0.021

4.475 ± 0.016

6.071 ± 0.015

8.055 ± 0.023

5.857 ± 0.016

5.874 ± 0.015

1.415 ± 0.007

2.756 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski