Rheinheimera sp. A13L

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Chromatiaceae; Rheinheimera; unclassified Rheinheimera

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3941 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F7NTZ5|F7NTZ5_9GAMM Metalloendopeptidase-like membrane protein OS=Rheinheimera sp. A13L OX=506534 GN=Rhein_1236 PE=4 SV=1
MM1 pKa = 7.81KK2 pKa = 10.19IGLFYY7 pKa = 11.11GSTTCYY13 pKa = 10.16TEE15 pKa = 3.93IVAEE19 pKa = 4.54KK20 pKa = 10.33IQVLLSADD28 pKa = 3.46PSLPDD33 pKa = 3.66GSSVTLHH40 pKa = 6.47NIKK43 pKa = 10.29DD44 pKa = 3.59QPLAMMADD52 pKa = 3.85YY53 pKa = 11.2DD54 pKa = 4.55LLILGISTWDD64 pKa = 3.44FGEE67 pKa = 4.43LQEE70 pKa = 5.27DD71 pKa = 4.61WEE73 pKa = 4.39AHH75 pKa = 5.59WDD77 pKa = 4.16DD78 pKa = 3.64IAEE81 pKa = 4.08VDD83 pKa = 3.84LSGKK87 pKa = 9.57IVAIYY92 pKa = 10.73GMGDD96 pKa = 3.17QLGYY100 pKa = 11.12AEE102 pKa = 4.64WFQDD106 pKa = 3.53AVGMLHH112 pKa = 6.42QAIAQQDD119 pKa = 4.3CIRR122 pKa = 11.84IGFWPVEE129 pKa = 4.16GYY131 pKa = 11.16DD132 pKa = 4.36FIASKK137 pKa = 10.9AVTEE141 pKa = 4.45DD142 pKa = 4.0GEE144 pKa = 4.5WFYY147 pKa = 11.9GLALDD152 pKa = 5.72EE153 pKa = 4.62EE154 pKa = 4.54NQYY157 pKa = 11.83DD158 pKa = 4.64LTDD161 pKa = 3.25QRR163 pKa = 11.84LNQWLTQLMNEE174 pKa = 5.09LIALL178 pKa = 4.38

Molecular weight: 20.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F7NQT2|F7NQT2_9GAMM Putative hemoglobin-like oxygen-binding protein OS=Rheinheimera sp. A13L OX=506534 GN=Rhein_0300 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.3RR12 pKa = 11.84ARR14 pKa = 11.84VHH16 pKa = 6.23GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.42NGRR28 pKa = 11.84QVIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.69GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3941

0

3941

1348315

27

5933

342.1

37.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.684 ± 0.043

0.984 ± 0.013

5.223 ± 0.031

5.551 ± 0.032

4.128 ± 0.026

6.568 ± 0.039

2.207 ± 0.018

5.276 ± 0.029

4.856 ± 0.032

11.782 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.318 ± 0.02

3.645 ± 0.03

4.102 ± 0.025

6.154 ± 0.058

4.455 ± 0.032

6.575 ± 0.033

5.143 ± 0.041

6.905 ± 0.037

1.393 ± 0.016

3.052 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski