Mycobacterium phage Gancho

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Gilesvirus; unclassified Gilesvirus

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385UD33|A0A385UD33_9CAUD Uncharacterized protein OS=Mycobacterium phage Gancho OX=2301613 GN=36 PE=4 SV=1
MM1 pKa = 7.33TMRR4 pKa = 11.84VQQVDD9 pKa = 2.84VDD11 pKa = 3.73MSGWCPVTRR20 pKa = 11.84LYY22 pKa = 9.99RR23 pKa = 11.84TEE25 pKa = 4.47DD26 pKa = 3.61GEE28 pKa = 4.6HH29 pKa = 6.35IAVTVHH35 pKa = 7.78DD36 pKa = 4.46YY37 pKa = 7.74MTANGSVAAFPATAEE52 pKa = 4.17GFAVDD57 pKa = 5.35LTPLWSTADD66 pKa = 3.6AVTHH70 pKa = 5.78EE71 pKa = 5.05AALAMHH77 pKa = 7.06GYY79 pKa = 9.68ILEE82 pKa = 4.49DD83 pKa = 3.18

Molecular weight: 9.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385UD72|A0A385UD72_9CAUD Uncharacterized protein OS=Mycobacterium phage Gancho OX=2301613 GN=77 PE=4 SV=1
MM1 pKa = 7.86KK2 pKa = 10.63YY3 pKa = 10.93GDD5 pKa = 3.89GTVKK9 pKa = 10.2GHH11 pKa = 6.2RR12 pKa = 11.84LEE14 pKa = 4.87NRR16 pKa = 11.84TDD18 pKa = 3.53PAVRR22 pKa = 11.84DD23 pKa = 3.74ALTRR27 pKa = 11.84PCPLCGAAPRR37 pKa = 11.84VLCIRR42 pKa = 11.84PRR44 pKa = 11.84WCRR47 pKa = 11.84RR48 pKa = 11.84LVHH51 pKa = 6.49FARR54 pKa = 11.84CRR56 pKa = 11.84FRR58 pKa = 11.84EE59 pKa = 4.24DD60 pKa = 3.03VSS62 pKa = 3.52

Molecular weight: 7.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

86

0

86

17383

47

1361

202.1

21.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.709 ± 0.462

1.047 ± 0.161

7.289 ± 0.259

4.936 ± 0.252

2.52 ± 0.151

9.279 ± 0.538

2.163 ± 0.207

4.361 ± 0.243

2.617 ± 0.207

6.478 ± 0.261

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.244 ± 0.132

3.124 ± 0.184

6.961 ± 0.294

2.75 ± 0.21

7.887 ± 0.466

4.107 ± 0.197

7.07 ± 0.273

7.352 ± 0.249

1.835 ± 0.13

2.272 ± 0.116
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski