Pseudoalteromonas phage C5a

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Catalunyavirus; Pseudoalteromonas virus C5a

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L5C2C1|A0A1L5C2C1_9CAUD Portal protein OS=Pseudoalteromonas phage C5a OX=1916107 GN=C5a_24 PE=3 SV=1
MM1 pKa = 7.46TSIKK5 pKa = 10.86DD6 pKa = 3.32QDD8 pKa = 3.55LSKK11 pKa = 11.31NQLLVKK17 pKa = 10.56NIVEE21 pKa = 4.18HH22 pKa = 7.38AIDD25 pKa = 3.55QANFTIKK32 pKa = 10.43NLSKK36 pKa = 10.63RR37 pKa = 11.84PTVAMLMEE45 pKa = 4.97CEE47 pKa = 3.98NCLTDD52 pKa = 4.54FMPVVKK58 pKa = 10.45LIADD62 pKa = 4.25DD63 pKa = 4.36YY64 pKa = 11.49IEE66 pKa = 4.28YY67 pKa = 10.87APIYY71 pKa = 10.22DD72 pKa = 3.93QMCAAIDD79 pKa = 3.66AVQMGEE85 pKa = 4.07DD86 pKa = 3.29LVEE89 pKa = 4.38IEE91 pKa = 4.61FAA93 pKa = 4.22

Molecular weight: 10.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L5C2C5|A0A1L5C2C5_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage C5a OX=1916107 GN=C5a_43 PE=4 SV=1
MM1 pKa = 7.32SLVITPNSKK10 pKa = 9.43QALSAKK16 pKa = 10.08HH17 pKa = 5.41QLQLLALPAGKK28 pKa = 9.55RR29 pKa = 11.84VRR31 pKa = 11.84VLKK34 pKa = 9.9TLGRR38 pKa = 11.84YY39 pKa = 7.06EE40 pKa = 4.1RR41 pKa = 11.84KK42 pKa = 9.52LARR45 pKa = 11.84QRR47 pKa = 11.84IRR49 pKa = 11.84AQNTVEE55 pKa = 4.08GNKK58 pKa = 9.42FASRR62 pKa = 11.84TDD64 pKa = 3.34GKK66 pKa = 8.48KK67 pKa = 8.7TKK69 pKa = 8.54MLKK72 pKa = 10.5RR73 pKa = 11.84MGKK76 pKa = 6.8TLEE79 pKa = 4.45PYY81 pKa = 10.14VKK83 pKa = 10.24GGKK86 pKa = 9.39RR87 pKa = 11.84LEE89 pKa = 4.47LKK91 pKa = 10.49HH92 pKa = 6.46KK93 pKa = 10.68AGLTGRR99 pKa = 11.84IAALHH104 pKa = 5.28QEE106 pKa = 4.5GGSEE110 pKa = 4.09QMTSTRR116 pKa = 11.84MSRR119 pKa = 11.84IHH121 pKa = 6.87GKK123 pKa = 9.18PDD125 pKa = 3.07YY126 pKa = 10.07KK127 pKa = 11.01APCTRR132 pKa = 11.84SQAKK136 pKa = 9.73ALAAEE141 pKa = 5.51GYY143 pKa = 9.16KK144 pKa = 10.13VRR146 pKa = 11.84RR147 pKa = 11.84AKK149 pKa = 10.82GKK151 pKa = 8.34GYY153 pKa = 10.23RR154 pKa = 11.84RR155 pKa = 11.84ATIKK159 pKa = 10.57EE160 pKa = 3.84IAGSLTHH167 pKa = 6.91GKK169 pKa = 8.23ATLVLSKK176 pKa = 10.91LRR178 pKa = 11.84GQKK181 pKa = 9.15SRR183 pKa = 11.84KK184 pKa = 8.41SWPIPVKK191 pKa = 10.52ARR193 pKa = 11.84PFLGDD198 pKa = 2.97TTANVQAQLATILNQLNKK216 pKa = 10.33RR217 pKa = 11.84GG218 pKa = 3.54

Molecular weight: 24.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

10259

39

1046

223.0

24.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.202 ± 0.458

0.877 ± 0.147

6.092 ± 0.258

5.8 ± 0.202

4.074 ± 0.277

6.531 ± 0.326

2.115 ± 0.197

6.209 ± 0.31

6.57 ± 0.363

8.539 ± 0.284

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.398 ± 0.24

5.585 ± 0.341

3.802 ± 0.263

4.494 ± 0.277

4.396 ± 0.37

6.472 ± 0.311

5.975 ± 0.245

6.492 ± 0.295

1.374 ± 0.101

3.002 ± 0.156
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski