Microbacterium phage Efeko

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 28 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A386KP29|A0A386KP29_9CAUD HNH endonuclease OS=Microbacterium phage Efeko OX=2315704 GN=27 PE=4 SV=1
MM1 pKa = 6.75TTFLRR6 pKa = 11.84IDD8 pKa = 3.85PEE10 pKa = 4.16TGQLIEE16 pKa = 4.62CGSRR20 pKa = 11.84TVVCEE25 pKa = 3.62TAGCEE30 pKa = 3.79NVGISIEE37 pKa = 4.39VADD40 pKa = 5.0DD41 pKa = 3.69PNGAVVCGPCGRR53 pKa = 11.84WIIAPPTEE61 pKa = 4.23QEE63 pKa = 4.17TPAA66 pKa = 5.02

Molecular weight: 6.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A386KLN1|A0A386KLN1_9CAUD Minor tail protein OS=Microbacterium phage Efeko OX=2315704 GN=14 PE=4 SV=1
MM1 pKa = 7.92TDD3 pKa = 3.88PKK5 pKa = 10.7PLTRR9 pKa = 11.84RR10 pKa = 11.84EE11 pKa = 3.58RR12 pKa = 11.84RR13 pKa = 11.84LQLRR17 pKa = 11.84AKK19 pKa = 8.04TEE21 pKa = 4.48RR22 pKa = 11.84IATPALRR29 pKa = 11.84RR30 pKa = 11.84WAYY33 pKa = 9.83GVAAAAVAAGVGFGWLPAGSAALLLPLLGALFYY66 pKa = 11.14VDD68 pKa = 5.2DD69 pKa = 5.06AGEE72 pKa = 3.96PRR74 pKa = 11.84AA75 pKa = 4.23

Molecular weight: 8.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

28

0

28

5499

36

699

196.4

21.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.184 ± 0.48

0.491 ± 0.194

5.619 ± 0.396

5.383 ± 0.478

2.855 ± 0.243

9.238 ± 0.426

1.4 ± 0.211

4.455 ± 0.358

2.164 ± 0.243

9.493 ± 0.813

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.146 ± 0.262

2.873 ± 0.24

5.055 ± 0.372

3.964 ± 0.328

7.092 ± 0.704

6.219 ± 0.55

6.947 ± 0.362

7.674 ± 0.485

1.418 ± 0.226

2.328 ± 0.281
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski