Clostridium sp. CAG:43

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2273 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7B5B9|R7B5B9_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:43 OX=1262805 GN=BN653_00114 PE=4 SV=1
MM1 pKa = 7.79EE2 pKa = 4.78NQFLPIGTVVRR13 pKa = 11.84LKK15 pKa = 10.92EE16 pKa = 3.9STADD20 pKa = 3.54VMIAGYY26 pKa = 10.43LPINPSQPNYY36 pKa = 8.63VWDD39 pKa = 3.81YY40 pKa = 11.65SGFKK44 pKa = 10.62YY45 pKa = 10.37PIGYY49 pKa = 9.49VDD51 pKa = 3.85NNEE54 pKa = 4.18VYY56 pKa = 10.74CFDD59 pKa = 3.92GEE61 pKa = 4.36QIEE64 pKa = 4.6EE65 pKa = 4.07IQAIGYY71 pKa = 7.72QDD73 pKa = 3.72KK74 pKa = 10.53EE75 pKa = 4.1QLAFAAEE82 pKa = 4.2LEE84 pKa = 4.43EE85 pKa = 4.08MAEE88 pKa = 4.19EE89 pKa = 4.16VRR91 pKa = 11.84IEE93 pKa = 3.86AQMDD97 pKa = 3.52DD98 pKa = 3.63VEE100 pKa = 4.98YY101 pKa = 10.29PDD103 pKa = 3.46EE104 pKa = 4.41TEE106 pKa = 4.29EE107 pKa = 4.3EE108 pKa = 4.31EE109 pKa = 4.65KK110 pKa = 11.16

Molecular weight: 12.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7A1V7|R7A1V7_9CLOT RNA methyltransferase TrmH family OS=Clostridium sp. CAG:43 OX=1262805 GN=BN653_00647 PE=4 SV=1
MM1 pKa = 7.22AQIFLDD7 pKa = 3.72HH8 pKa = 6.29RR9 pKa = 11.84QGRR12 pKa = 11.84HH13 pKa = 4.63RR14 pKa = 11.84SRR16 pKa = 11.84QVRR19 pKa = 11.84HH20 pKa = 5.83RR21 pKa = 11.84SPRR24 pKa = 11.84LLADD28 pKa = 4.16LPRR31 pKa = 11.84LPKK34 pKa = 10.47LYY36 pKa = 10.59FPLLRR41 pKa = 11.84LQYY44 pKa = 10.95NITQKK49 pKa = 10.46EE50 pKa = 4.15LHH52 pKa = 6.66PLCLLQLSLFGQEE65 pKa = 4.04QLLTAFFIPRR75 pKa = 11.84IRR77 pKa = 11.84PRR79 pKa = 11.84VTLGEE84 pKa = 4.18IVDD87 pKa = 3.77LHH89 pKa = 6.81HH90 pKa = 7.11ILGNSTVRR98 pKa = 11.84LARR101 pKa = 11.84RR102 pKa = 11.84IRR104 pKa = 11.84VHH106 pKa = 6.58KK107 pKa = 10.45LLIPFPAVAVIIFQQPIHH125 pKa = 6.88HH126 pKa = 6.79ISAHH130 pKa = 4.9QKK132 pKa = 9.9HH133 pKa = 6.22FSLIADD139 pKa = 4.07PEE141 pKa = 4.26CRR143 pKa = 11.84IDD145 pKa = 3.69VQPVKK150 pKa = 10.23MFPHH154 pKa = 7.19DD155 pKa = 3.8MQAEE159 pKa = 4.19AVDD162 pKa = 3.96RR163 pKa = 11.84RR164 pKa = 11.84DD165 pKa = 4.48LGKK168 pKa = 10.83LNQNCLLLEE177 pKa = 3.9MFIFRR182 pKa = 11.84LRR184 pKa = 11.84FQFFLNRR191 pKa = 11.84RR192 pKa = 11.84LDD194 pKa = 3.94PLPHH198 pKa = 6.89LRR200 pKa = 11.84RR201 pKa = 11.84CRR203 pKa = 11.84PGKK206 pKa = 10.21SHH208 pKa = 7.66DD209 pKa = 3.64KK210 pKa = 9.45QAVYY214 pKa = 10.43VLRR217 pKa = 11.84VLPLRR222 pKa = 11.84DD223 pKa = 4.12HH224 pKa = 7.29LDD226 pKa = 3.72DD227 pKa = 5.81PLNQHH232 pKa = 6.72CGLAASGRR240 pKa = 11.84CRR242 pKa = 11.84HH243 pKa = 5.52QEE245 pKa = 3.65AFLPPLNYY253 pKa = 10.51LLLFPGPIYY262 pKa = 10.56CHH264 pKa = 6.54FSPFPFSS271 pKa = 3.3

Molecular weight: 31.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2273

0

2273

693025

30

1862

304.9

34.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.312 ± 0.054

1.49 ± 0.022

5.418 ± 0.04

7.792 ± 0.066

3.938 ± 0.039

7.617 ± 0.046

1.818 ± 0.023

6.731 ± 0.048

6.238 ± 0.046

9.088 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.167 ± 0.026

3.83 ± 0.037

3.528 ± 0.028

3.323 ± 0.029

4.96 ± 0.039

5.677 ± 0.042

5.274 ± 0.04

6.957 ± 0.042

0.955 ± 0.019

3.87 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski