Thermoanaerobaculum aquaticum

Taxonomy: cellular organisms; Bacteria; Acidobacteria; Thermoanaerobaculia; Thermoanaerobaculales; Thermoanaerobaculaceae; Thermoanaerobaculum

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2252 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A062XM57|A0A062XM57_9BACT ABC transmembrane type-1 domain-containing protein OS=Thermoanaerobaculum aquaticum OX=1312852 GN=EG19_05510 PE=3 SV=1
MM1 pKa = 7.76ADD3 pKa = 3.42KK4 pKa = 10.55TNKK7 pKa = 9.53VPLNVPGKK15 pKa = 8.57WYY17 pKa = 10.97VDD19 pKa = 3.75TSCIDD24 pKa = 3.49CDD26 pKa = 3.95VCRR29 pKa = 11.84TTAPNNFKK37 pKa = 11.01ANEE40 pKa = 4.08DD41 pKa = 3.25EE42 pKa = 4.89GYY44 pKa = 10.57SYY46 pKa = 11.38VYY48 pKa = 9.78KK49 pKa = 10.63QPEE52 pKa = 4.27TPEE55 pKa = 4.61EE56 pKa = 3.8EE57 pKa = 4.51AQCQEE62 pKa = 4.42AKK64 pKa = 10.52ASCPVEE70 pKa = 5.03AIGDD74 pKa = 4.17DD75 pKa = 3.79GDD77 pKa = 3.75EE78 pKa = 4.08

Molecular weight: 8.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A062XXP5|A0A062XXP5_9BACT Uncharacterized protein OS=Thermoanaerobaculum aquaticum OX=1312852 GN=EG19_09350 PE=4 SV=1
MM1 pKa = 7.5SFRR4 pKa = 11.84HH5 pKa = 5.57AFSLAVLPGLALVLASFAGWVPAARR30 pKa = 11.84DD31 pKa = 3.48NPTYY35 pKa = 10.19FVPLRR40 pKa = 11.84AQLARR45 pKa = 11.84VFTGEE50 pKa = 3.76ASPWLNPQVGCGEE63 pKa = 4.18PFFANPQSALLYY75 pKa = 8.84PPAWGALLLPPEE87 pKa = 3.99QAIGVEE93 pKa = 4.37VGLHH97 pKa = 6.2LMLLALGVARR107 pKa = 11.84LARR110 pKa = 11.84RR111 pKa = 11.84LGGTPTGSLAAAWGAALSGPVLSSAGMLNNLEE143 pKa = 4.06TAAWLPWVWDD153 pKa = 3.56AALSGRR159 pKa = 11.84FGVLALTVTGSFLAAEE175 pKa = 4.43PVLALLGAAGAVWLLPRR192 pKa = 11.84WQAVRR197 pKa = 11.84AVLLGFALCSVQALPMAFWIAGGDD221 pKa = 3.6RR222 pKa = 11.84GPDD225 pKa = 3.21KK226 pKa = 10.74PLEE229 pKa = 4.25AVSLGGVSLGEE240 pKa = 4.06LPALVVPGFPLPPVEE255 pKa = 5.03VRR257 pKa = 11.84FLPIIALPLWLFLALGSLRR276 pKa = 11.84RR277 pKa = 11.84GEE279 pKa = 4.14TARR282 pKa = 11.84TRR284 pKa = 11.84LASLAAVFTFLAVLPALPWGDD305 pKa = 4.04ALWAGLSFGLVRR317 pKa = 11.84LPGRR321 pKa = 11.84FLIPATVALAAVAGSGKK338 pKa = 10.17LPQSRR343 pKa = 11.84KK344 pKa = 7.14WVATALGLGAVGAVVSRR361 pKa = 11.84EE362 pKa = 3.59PWLAAGQGVLAALAPWGMGWAAAGSLFLATYY393 pKa = 7.4TVPVLQLQKK402 pKa = 10.15WKK404 pKa = 9.96PEE406 pKa = 3.8PVLCLSAQSAGRR418 pKa = 11.84LYY420 pKa = 9.77PLPVDD425 pKa = 4.43GVQLRR430 pKa = 11.84WTLEE434 pKa = 3.61RR435 pKa = 11.84GTRR438 pKa = 11.84GAASLGWGYY447 pKa = 11.33SVLLDD452 pKa = 3.53GRR454 pKa = 11.84SLARR458 pKa = 11.84SFAPVTNRR466 pKa = 11.84ALAQHH471 pKa = 7.25LAQADD476 pKa = 3.8RR477 pKa = 11.84GFSEE481 pKa = 4.4GWWWVSALGAKK492 pKa = 9.14TMVGLHH498 pKa = 7.18PIPGYY503 pKa = 9.96PPLCQRR509 pKa = 11.84DD510 pKa = 4.09KK511 pKa = 11.37LWVFRR516 pKa = 11.84NPSAFPLWAVVSHH529 pKa = 6.77LPTPGEE535 pKa = 4.0LPVPAGEE542 pKa = 4.74AVLKK546 pKa = 10.45GQGRR550 pKa = 11.84TSWQFQVRR558 pKa = 11.84AQTNGVFLWLFAPDD572 pKa = 3.96PGWRR576 pKa = 11.84FVVDD580 pKa = 4.12GKK582 pKa = 10.46KK583 pKa = 10.26VKK585 pKa = 9.85PIRR588 pKa = 11.84GAGILQGVPVAAGDD602 pKa = 3.64HH603 pKa = 5.49EE604 pKa = 4.61VRR606 pKa = 11.84VVYY609 pKa = 10.3RR610 pKa = 11.84PPGLMAGLVVSLASLFLLGVLWRR633 pKa = 11.84RR634 pKa = 3.6

Molecular weight: 67.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2252

0

2252

781730

49

1332

347.1

37.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.564 ± 0.063

0.939 ± 0.02

3.995 ± 0.035

6.877 ± 0.058

4.051 ± 0.037

8.378 ± 0.044

1.979 ± 0.024

3.183 ± 0.035

3.404 ± 0.035

12.106 ± 0.075

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.631 ± 0.019

2.102 ± 0.029

6.09 ± 0.042

3.807 ± 0.029

7.379 ± 0.047

5.046 ± 0.04

4.641 ± 0.04

8.976 ± 0.045

1.716 ± 0.029

2.135 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski