Amino acid dipepetide frequency for Microbacterium phage Hendrix

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.914AlaAla: 12.914 ± 1.352
0.641AlaCys: 0.641 ± 0.16
5.544AlaAsp: 5.544 ± 0.488
8.428AlaGlu: 8.428 ± 0.702
2.948AlaPhe: 2.948 ± 0.456
7.242AlaGly: 7.242 ± 0.625
2.179AlaHis: 2.179 ± 0.277
4.903AlaIle: 4.903 ± 0.419
3.813AlaLys: 3.813 ± 0.547
9.197AlaLeu: 9.197 ± 0.585
2.852AlaMet: 2.852 ± 0.328
2.243AlaAsn: 2.243 ± 0.27
4.935AlaPro: 4.935 ± 0.421
4.326AlaGln: 4.326 ± 0.448
6.345AlaArg: 6.345 ± 0.593
5.672AlaSer: 5.672 ± 0.53
7.05AlaThr: 7.05 ± 0.66
7.819AlaVal: 7.819 ± 0.99
1.73AlaTrp: 1.73 ± 0.298
2.628AlaTyr: 2.628 ± 0.26
0.0AlaXaa: 0.0 ± 0.0
Cys
0.481CysAla: 0.481 ± 0.133
0.032CysCys: 0.032 ± 0.033
0.577CysAsp: 0.577 ± 0.146
0.513CysGlu: 0.513 ± 0.124
0.224CysPhe: 0.224 ± 0.089
1.442CysGly: 1.442 ± 0.234
0.352CysHis: 0.352 ± 0.104
0.256CysIle: 0.256 ± 0.086
0.128CysLys: 0.128 ± 0.066
0.641CysLeu: 0.641 ± 0.154
0.096CysMet: 0.096 ± 0.051
0.256CysAsn: 0.256 ± 0.095
0.801CysPro: 0.801 ± 0.137
0.256CysGln: 0.256 ± 0.116
0.417CysArg: 0.417 ± 0.118
0.481CysSer: 0.481 ± 0.131
0.609CysThr: 0.609 ± 0.136
0.545CysVal: 0.545 ± 0.136
0.128CysTrp: 0.128 ± 0.06
0.16CysTyr: 0.16 ± 0.066
0.0CysXaa: 0.0 ± 0.0
Asp
5.832AspAla: 5.832 ± 0.594
0.513AspCys: 0.513 ± 0.12
4.871AspAsp: 4.871 ± 0.479
5.095AspGlu: 5.095 ± 0.513
1.955AspPhe: 1.955 ± 0.244
5.864AspGly: 5.864 ± 0.444
1.218AspHis: 1.218 ± 0.224
2.628AspIle: 2.628 ± 0.342
1.923AspLys: 1.923 ± 0.229
4.903AspLeu: 4.903 ± 0.378
1.314AspMet: 1.314 ± 0.187
1.698AspAsn: 1.698 ± 0.193
4.262AspPro: 4.262 ± 0.493
2.467AspGln: 2.467 ± 0.259
3.749AspArg: 3.749 ± 0.377
3.653AspSer: 3.653 ± 0.271
2.98AspThr: 2.98 ± 0.288
4.967AspVal: 4.967 ± 0.39
1.186AspTrp: 1.186 ± 0.187
2.115AspTyr: 2.115 ± 0.392
0.0AspXaa: 0.0 ± 0.0
Glu
7.819GluAla: 7.819 ± 0.594
0.449GluCys: 0.449 ± 0.112
5.255GluAsp: 5.255 ± 0.406
6.505GluGlu: 6.505 ± 0.691
1.762GluPhe: 1.762 ± 0.238
4.614GluGly: 4.614 ± 0.494
1.73GluHis: 1.73 ± 0.241
3.333GluIle: 3.333 ± 0.382
3.461GluLys: 3.461 ± 0.418
6.313GluLeu: 6.313 ± 0.539
2.147GluMet: 2.147 ± 0.32
2.435GluAsn: 2.435 ± 0.263
3.621GluPro: 3.621 ± 0.459
3.621GluGln: 3.621 ± 0.387
5.191GluArg: 5.191 ± 0.447
3.237GluSer: 3.237 ± 0.27
3.333GluThr: 3.333 ± 0.345
5.544GluVal: 5.544 ± 0.514
1.25GluTrp: 1.25 ± 0.219
1.73GluTyr: 1.73 ± 0.239
0.0GluXaa: 0.0 ± 0.0
Phe
2.66PheAla: 2.66 ± 0.257
0.192PheCys: 0.192 ± 0.073
2.339PheAsp: 2.339 ± 0.277
1.795PheGlu: 1.795 ± 0.245
0.801PhePhe: 0.801 ± 0.175
2.532PheGly: 2.532 ± 0.305
0.449PheHis: 0.449 ± 0.11
1.666PheIle: 1.666 ± 0.305
1.025PheLys: 1.025 ± 0.18
2.211PheLeu: 2.211 ± 0.267
0.641PheMet: 0.641 ± 0.139
0.929PheAsn: 0.929 ± 0.171
1.057PhePro: 1.057 ± 0.197
1.218PheGln: 1.218 ± 0.187
1.73PheArg: 1.73 ± 0.241
2.019PheSer: 2.019 ± 0.314
2.115PheThr: 2.115 ± 0.26
2.115PheVal: 2.115 ± 0.301
0.801PheTrp: 0.801 ± 0.146
0.352PheTyr: 0.352 ± 0.091
0.0PheXaa: 0.0 ± 0.0
Gly
6.858GlyAla: 6.858 ± 0.53
0.673GlyCys: 0.673 ± 0.169
4.294GlyAsp: 4.294 ± 0.336
4.743GlyGlu: 4.743 ± 0.448
2.916GlyPhe: 2.916 ± 0.321
6.345GlyGly: 6.345 ± 0.734
1.698GlyHis: 1.698 ± 0.22
3.205GlyIle: 3.205 ± 0.446
3.269GlyLys: 3.269 ± 0.446
6.217GlyLeu: 6.217 ± 0.492
2.243GlyMet: 2.243 ± 0.209
2.564GlyAsn: 2.564 ± 0.304
3.205GlyPro: 3.205 ± 0.301
2.66GlyGln: 2.66 ± 0.276
4.358GlyArg: 4.358 ± 0.476
4.935GlySer: 4.935 ± 0.36
6.089GlyThr: 6.089 ± 1.055
5.896GlyVal: 5.896 ± 0.382
2.243GlyTrp: 2.243 ± 0.306
2.596GlyTyr: 2.596 ± 0.328
0.0GlyXaa: 0.0 ± 0.0
His
1.762HisAla: 1.762 ± 0.263
0.385HisCys: 0.385 ± 0.116
1.57HisAsp: 1.57 ± 0.206
1.346HisGlu: 1.346 ± 0.216
0.577HisPhe: 0.577 ± 0.121
1.57HisGly: 1.57 ± 0.278
0.385HisHis: 0.385 ± 0.122
1.122HisIle: 1.122 ± 0.174
0.385HisLys: 0.385 ± 0.113
1.795HisLeu: 1.795 ± 0.234
0.385HisMet: 0.385 ± 0.109
0.609HisAsn: 0.609 ± 0.131
1.378HisPro: 1.378 ± 0.291
0.609HisGln: 0.609 ± 0.149
1.795HisArg: 1.795 ± 0.319
1.314HisSer: 1.314 ± 0.22
0.993HisThr: 0.993 ± 0.183
1.698HisVal: 1.698 ± 0.272
0.32HisTrp: 0.32 ± 0.094
0.769HisTyr: 0.769 ± 0.18
0.0HisXaa: 0.0 ± 0.0
Ile
4.55IleAla: 4.55 ± 0.444
0.481IleCys: 0.481 ± 0.129
4.134IleAsp: 4.134 ± 0.353
4.422IleGlu: 4.422 ± 0.426
1.025IlePhe: 1.025 ± 0.188
4.006IleGly: 4.006 ± 0.513
1.057IleHis: 1.057 ± 0.196
2.403IleIle: 2.403 ± 0.304
1.538IleLys: 1.538 ± 0.233
2.756IleLeu: 2.756 ± 0.343
1.122IleMet: 1.122 ± 0.245
1.474IleAsn: 1.474 ± 0.195
2.948IlePro: 2.948 ± 0.358
1.538IleGln: 1.538 ± 0.231
2.628IleArg: 2.628 ± 0.239
2.371IleSer: 2.371 ± 0.382
3.269IleThr: 3.269 ± 0.33
4.518IleVal: 4.518 ± 0.384
0.897IleTrp: 0.897 ± 0.163
1.186IleTyr: 1.186 ± 0.215
0.0IleXaa: 0.0 ± 0.0
Lys
4.23LysAla: 4.23 ± 0.415
0.288LysCys: 0.288 ± 0.087
1.795LysAsp: 1.795 ± 0.218
2.467LysGlu: 2.467 ± 0.332
0.897LysPhe: 0.897 ± 0.18
2.403LysGly: 2.403 ± 0.306
0.865LysHis: 0.865 ± 0.194
1.987LysIle: 1.987 ± 0.252
2.147LysLys: 2.147 ± 0.329
3.365LysLeu: 3.365 ± 0.329
0.993LysMet: 0.993 ± 0.206
1.506LysAsn: 1.506 ± 0.191
2.435LysPro: 2.435 ± 0.385
1.378LysGln: 1.378 ± 0.208
2.98LysArg: 2.98 ± 0.343
1.923LysSer: 1.923 ± 0.258
2.211LysThr: 2.211 ± 0.296
2.467LysVal: 2.467 ± 0.282
0.641LysTrp: 0.641 ± 0.151
1.057LysTyr: 1.057 ± 0.188
0.0LysXaa: 0.0 ± 0.0
Leu
7.979LeuAla: 7.979 ± 0.605
0.737LeuCys: 0.737 ± 0.172
5.512LeuAsp: 5.512 ± 0.48
6.729LeuGlu: 6.729 ± 0.474
1.474LeuPhe: 1.474 ± 0.215
5.48LeuGly: 5.48 ± 0.402
1.474LeuHis: 1.474 ± 0.254
4.038LeuIle: 4.038 ± 0.43
3.14LeuLys: 3.14 ± 0.377
5.063LeuLeu: 5.063 ± 0.504
2.307LeuMet: 2.307 ± 0.246
2.564LeuAsn: 2.564 ± 0.287
4.006LeuPro: 4.006 ± 0.377
2.66LeuGln: 2.66 ± 0.319
5.319LeuArg: 5.319 ± 0.534
5.031LeuSer: 5.031 ± 0.507
5.992LeuThr: 5.992 ± 0.472
5.384LeuVal: 5.384 ± 0.499
1.474LeuTrp: 1.474 ± 0.188
1.762LeuTyr: 1.762 ± 0.24
0.0LeuXaa: 0.0 ± 0.0
Met
2.788MetAla: 2.788 ± 0.302
0.16MetCys: 0.16 ± 0.061
1.378MetAsp: 1.378 ± 0.209
1.314MetGlu: 1.314 ± 0.229
0.609MetPhe: 0.609 ± 0.15
1.666MetGly: 1.666 ± 0.306
0.481MetHis: 0.481 ± 0.154
1.282MetIle: 1.282 ± 0.196
0.865MetLys: 0.865 ± 0.172
1.73MetLeu: 1.73 ± 0.23
1.09MetMet: 1.09 ± 0.188
1.41MetAsn: 1.41 ± 0.245
1.602MetPro: 1.602 ± 0.214
1.025MetGln: 1.025 ± 0.152
1.762MetArg: 1.762 ± 0.291
2.275MetSer: 2.275 ± 0.272
2.628MetThr: 2.628 ± 0.269
1.538MetVal: 1.538 ± 0.241
0.288MetTrp: 0.288 ± 0.095
0.481MetTyr: 0.481 ± 0.109
0.0MetXaa: 0.0 ± 0.0
Asn
3.269AsnAla: 3.269 ± 0.463
0.352AsnCys: 0.352 ± 0.11
1.698AsnAsp: 1.698 ± 0.271
2.179AsnGlu: 2.179 ± 0.301
0.801AsnPhe: 0.801 ± 0.196
3.493AsnGly: 3.493 ± 0.47
0.513AsnHis: 0.513 ± 0.131
1.282AsnIle: 1.282 ± 0.236
0.769AsnLys: 0.769 ± 0.193
1.827AsnLeu: 1.827 ± 0.249
0.545AsnMet: 0.545 ± 0.142
0.929AsnAsn: 0.929 ± 0.268
2.82AsnPro: 2.82 ± 0.531
1.122AsnGln: 1.122 ± 0.183
1.987AsnArg: 1.987 ± 0.238
1.442AsnSer: 1.442 ± 0.221
2.243AsnThr: 2.243 ± 0.314
2.147AsnVal: 2.147 ± 0.248
0.737AsnTrp: 0.737 ± 0.184
0.769AsnTyr: 0.769 ± 0.197
0.0AsnXaa: 0.0 ± 0.0
Pro
6.986ProAla: 6.986 ± 1.344
0.449ProCys: 0.449 ± 0.133
4.006ProAsp: 4.006 ± 0.496
4.903ProGlu: 4.903 ± 0.693
1.506ProPhe: 1.506 ± 0.21
4.486ProGly: 4.486 ± 0.727
1.346ProHis: 1.346 ± 0.273
3.108ProIle: 3.108 ± 0.292
2.467ProLys: 2.467 ± 0.369
3.461ProLeu: 3.461 ± 0.306
1.25ProMet: 1.25 ± 0.212
1.666ProAsn: 1.666 ± 0.42
2.724ProPro: 2.724 ± 0.409
1.506ProGln: 1.506 ± 0.187
3.108ProArg: 3.108 ± 0.437
3.397ProSer: 3.397 ± 0.354
4.07ProThr: 4.07 ± 0.627
3.269ProVal: 3.269 ± 0.343
0.897ProTrp: 0.897 ± 0.19
1.506ProTyr: 1.506 ± 0.223
0.0ProXaa: 0.0 ± 0.0
Gln
4.262GlnAla: 4.262 ± 0.398
0.288GlnCys: 0.288 ± 0.087
1.891GlnAsp: 1.891 ± 0.22
2.403GlnGlu: 2.403 ± 0.273
0.929GlnPhe: 0.929 ± 0.168
2.756GlnGly: 2.756 ± 0.257
0.929GlnHis: 0.929 ± 0.189
2.211GlnIle: 2.211 ± 0.252
1.795GlnLys: 1.795 ± 0.281
3.685GlnLeu: 3.685 ± 0.459
1.154GlnMet: 1.154 ± 0.223
1.346GlnAsn: 1.346 ± 0.234
1.634GlnPro: 1.634 ± 0.211
1.506GlnGln: 1.506 ± 0.248
2.467GlnArg: 2.467 ± 0.259
1.698GlnSer: 1.698 ± 0.221
2.467GlnThr: 2.467 ± 0.289
2.467GlnVal: 2.467 ± 0.27
0.865GlnTrp: 0.865 ± 0.157
0.961GlnTyr: 0.961 ± 0.163
0.0GlnXaa: 0.0 ± 0.0
Arg
6.954ArgAla: 6.954 ± 0.524
0.513ArgCys: 0.513 ± 0.169
3.813ArgAsp: 3.813 ± 0.387
4.262ArgGlu: 4.262 ± 0.472
2.275ArgPhe: 2.275 ± 0.29
4.903ArgGly: 4.903 ± 0.506
1.602ArgHis: 1.602 ± 0.271
3.685ArgIle: 3.685 ± 0.4
2.339ArgLys: 2.339 ± 0.307
5.352ArgLeu: 5.352 ± 0.446
1.827ArgMet: 1.827 ± 0.265
2.179ArgAsn: 2.179 ± 0.231
2.884ArgPro: 2.884 ± 0.324
2.532ArgGln: 2.532 ± 0.329
5.8ArgArg: 5.8 ± 0.534
3.781ArgSer: 3.781 ± 0.422
4.07ArgThr: 4.07 ± 0.391
4.326ArgVal: 4.326 ± 0.402
1.987ArgTrp: 1.987 ± 0.264
1.538ArgTyr: 1.538 ± 0.31
0.0ArgXaa: 0.0 ± 0.0
Ser
5.992SerAla: 5.992 ± 0.658
0.352SerCys: 0.352 ± 0.124
3.781SerAsp: 3.781 ± 0.358
3.877SerGlu: 3.877 ± 0.366
2.307SerPhe: 2.307 ± 0.203
4.006SerGly: 4.006 ± 0.44
0.897SerHis: 0.897 ± 0.169
2.948SerIle: 2.948 ± 0.335
2.083SerLys: 2.083 ± 0.29
5.063SerLeu: 5.063 ± 0.408
1.762SerMet: 1.762 ± 0.238
1.378SerAsn: 1.378 ± 0.238
2.852SerPro: 2.852 ± 0.415
1.987SerGln: 1.987 ± 0.236
4.23SerArg: 4.23 ± 0.459
3.237SerSer: 3.237 ± 0.564
3.653SerThr: 3.653 ± 0.577
4.294SerVal: 4.294 ± 0.411
1.282SerTrp: 1.282 ± 0.308
1.538SerTyr: 1.538 ± 0.264
0.0SerXaa: 0.0 ± 0.0
Thr
7.467ThrAla: 7.467 ± 0.779
0.769ThrCys: 0.769 ± 0.186
3.589ThrAsp: 3.589 ± 0.399
4.518ThrGlu: 4.518 ± 0.363
1.955ThrPhe: 1.955 ± 0.262
5.448ThrGly: 5.448 ± 0.591
1.057ThrHis: 1.057 ± 0.212
2.692ThrIle: 2.692 ± 0.467
2.532ThrLys: 2.532 ± 0.292
5.64ThrLeu: 5.64 ± 0.434
1.891ThrMet: 1.891 ± 0.245
1.859ThrAsn: 1.859 ± 0.299
5.48ThrPro: 5.48 ± 1.085
2.051ThrGln: 2.051 ± 0.245
3.974ThrArg: 3.974 ± 0.351
3.91ThrSer: 3.91 ± 0.81
4.967ThrThr: 4.967 ± 0.54
4.647ThrVal: 4.647 ± 0.454
1.442ThrTrp: 1.442 ± 0.237
1.474ThrTyr: 1.474 ± 0.336
0.0ThrXaa: 0.0 ± 0.0
Val
6.665ValAla: 6.665 ± 0.701
0.705ValCys: 0.705 ± 0.164
4.422ValAsp: 4.422 ± 0.388
4.999ValGlu: 4.999 ± 0.426
2.051ValPhe: 2.051 ± 0.258
4.711ValGly: 4.711 ± 0.555
1.698ValHis: 1.698 ± 0.228
3.781ValIle: 3.781 ± 0.393
2.756ValLys: 2.756 ± 0.329
4.999ValLeu: 4.999 ± 0.494
1.698ValMet: 1.698 ± 0.237
2.243ValAsn: 2.243 ± 0.293
5.191ValPro: 5.191 ± 1.288
3.429ValGln: 3.429 ± 0.325
4.935ValArg: 4.935 ± 0.442
4.358ValSer: 4.358 ± 0.362
5.64ValThr: 5.64 ± 0.535
4.582ValVal: 4.582 ± 0.393
1.474ValTrp: 1.474 ± 0.183
1.57ValTyr: 1.57 ± 0.251
0.0ValXaa: 0.0 ± 0.0
Trp
1.891TrpAla: 1.891 ± 0.268
0.256TrpCys: 0.256 ± 0.076
1.314TrpAsp: 1.314 ± 0.234
1.218TrpGlu: 1.218 ± 0.204
0.929TrpPhe: 0.929 ± 0.159
1.346TrpGly: 1.346 ± 0.176
0.449TrpHis: 0.449 ± 0.114
1.025TrpIle: 1.025 ± 0.22
0.865TrpLys: 0.865 ± 0.143
1.73TrpLeu: 1.73 ± 0.218
0.609TrpMet: 0.609 ± 0.162
0.801TrpAsn: 0.801 ± 0.391
0.993TrpPro: 0.993 ± 0.218
0.961TrpGln: 0.961 ± 0.177
1.57TrpArg: 1.57 ± 0.3
1.09TrpSer: 1.09 ± 0.213
1.346TrpThr: 1.346 ± 0.277
1.474TrpVal: 1.474 ± 0.211
0.545TrpTrp: 0.545 ± 0.133
0.449TrpTyr: 0.449 ± 0.117
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.083TyrAla: 2.083 ± 0.262
0.16TyrCys: 0.16 ± 0.076
1.41TyrAsp: 1.41 ± 0.234
1.634TyrGlu: 1.634 ± 0.266
0.705TyrPhe: 0.705 ± 0.166
2.692TyrGly: 2.692 ± 0.355
0.385TyrHis: 0.385 ± 0.11
0.769TyrIle: 0.769 ± 0.156
0.737TyrLys: 0.737 ± 0.166
2.179TyrLeu: 2.179 ± 0.235
0.417TyrMet: 0.417 ± 0.11
0.833TyrAsn: 0.833 ± 0.159
1.282TyrPro: 1.282 ± 0.229
0.865TyrGln: 0.865 ± 0.166
2.275TyrArg: 2.275 ± 0.304
1.698TyrSer: 1.698 ± 0.231
1.634TyrThr: 1.634 ± 0.282
2.211TyrVal: 2.211 ± 0.631
0.673TyrTrp: 0.673 ± 0.208
0.705TyrTyr: 0.705 ± 0.237
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 155 proteins (31207 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski