Methanobacterium virus PhiF3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 30 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9J4N5|A0A6B9J4N5_9CAUD Putative redox protein OS=Methanobacterium virus PhiF3 OX=2686281 PE=4 SV=1
MM1 pKa = 7.36APEE4 pKa = 4.75GVRR7 pKa = 11.84MVLYY11 pKa = 10.13NSRR14 pKa = 11.84VKK16 pKa = 10.81LYY18 pKa = 10.64DD19 pKa = 3.57FNVLDD24 pKa = 4.21SRR26 pKa = 11.84VTLEE30 pKa = 3.55AEE32 pKa = 4.46YY33 pKa = 10.98YY34 pKa = 10.15IEE36 pKa = 4.39TVDD39 pKa = 3.31EE40 pKa = 4.24ALVVKK45 pKa = 10.6RR46 pKa = 11.84RR47 pKa = 11.84LIEE50 pKa = 3.62SHH52 pKa = 6.62RR53 pKa = 11.84FFFKK57 pKa = 11.02DD58 pKa = 3.06EE59 pKa = 4.04FTEE62 pKa = 4.41TVVGEE67 pKa = 4.31EE68 pKa = 4.37VVSEE72 pKa = 4.36LNVEE76 pKa = 4.29DD77 pKa = 4.97EE78 pKa = 4.55FMSYY82 pKa = 11.04LNEE85 pKa = 4.11KK86 pKa = 9.94VGGIIDD92 pKa = 5.11LDD94 pKa = 3.88ASDD97 pKa = 4.45VKK99 pKa = 11.18SEE101 pKa = 6.04LITQYY106 pKa = 11.01PWLQDD111 pKa = 3.24KK112 pKa = 10.95LGEE115 pKa = 4.4PEE117 pKa = 4.59SEE119 pKa = 4.28PEE121 pKa = 4.06PEE123 pKa = 4.72LEE125 pKa = 4.55SEE127 pKa = 4.68PEE129 pKa = 4.07PEE131 pKa = 4.29SEE133 pKa = 3.88PDD135 pKa = 3.12AGEE138 pKa = 4.4GEE140 pKa = 4.71GVDD143 pKa = 4.68DD144 pKa = 3.75VHH146 pKa = 7.32KK147 pKa = 11.07

Molecular weight: 16.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9J446|A0A6B9J446_9CAUD Putative tail sheath protein OS=Methanobacterium virus PhiF3 OX=2686281 PE=4 SV=1
MM1 pKa = 7.71RR2 pKa = 11.84SEE4 pKa = 3.94STLTIKK10 pKa = 10.25IDD12 pKa = 3.14ITGYY16 pKa = 11.35KK17 pKa = 9.14MDD19 pKa = 4.07GLEE22 pKa = 3.99GKK24 pKa = 8.84IRR26 pKa = 11.84KK27 pKa = 7.72WAEE30 pKa = 3.46AGTQLSVRR38 pKa = 11.84HH39 pKa = 5.59FEE41 pKa = 4.18SLLVKK46 pKa = 7.77YY47 pKa = 8.87TPARR51 pKa = 11.84KK52 pKa = 9.5GRR54 pKa = 11.84GGGGQLRR61 pKa = 11.84ASLHH65 pKa = 5.01FRR67 pKa = 11.84KK68 pKa = 9.71QGRR71 pKa = 11.84LQFKK75 pKa = 8.16STPKK79 pKa = 10.82YY80 pKa = 10.18YY81 pKa = 9.38FTYY84 pKa = 10.17LDD86 pKa = 4.29KK87 pKa = 11.11GTGVYY92 pKa = 10.71GRR94 pKa = 11.84GSPIVPVRR102 pKa = 11.84KK103 pKa = 9.39KK104 pKa = 10.49ALAWHH109 pKa = 6.12PQNPLRR115 pKa = 11.84ACGRR119 pKa = 11.84GTGCIVRR126 pKa = 11.84KK127 pKa = 7.84SVRR130 pKa = 11.84GIRR133 pKa = 11.84PFRR136 pKa = 11.84IVDD139 pKa = 3.11KK140 pKa = 10.21ATRR143 pKa = 11.84GLQRR147 pKa = 11.84DD148 pKa = 3.3IEE150 pKa = 4.6RR151 pKa = 11.84IYY153 pKa = 11.34EE154 pKa = 4.01MVAEE158 pKa = 4.73KK159 pKa = 10.37IIGGG163 pKa = 3.81

Molecular weight: 18.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

30

0

30

9931

83

1537

331.0

37.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.881 ± 0.727

1.249 ± 0.396

5.941 ± 0.587

7.129 ± 0.783

3.776 ± 0.161

8.227 ± 0.643

1.581 ± 0.281

5.659 ± 0.337

6.465 ± 0.398

8.841 ± 0.274

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.437 ± 0.241

3.645 ± 0.239

4.582 ± 0.453

2.759 ± 0.247

5.86 ± 0.447

6.918 ± 0.59

6.203 ± 0.406

7.23 ± 0.397

1.43 ± 0.144

4.189 ± 0.489
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski